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The map label for this gene is cca [H]

Identifier: 16124663

GI number: 16124663

Start: 426391

End: 427632

Strand: Direct

Name: cca [H]

Synonym: CC_0408

Alternate gene names: 16124663

Gene position: 426391-427632 (Clockwise)

Preceding gene: 16124662

Following gene: 16124664

Centisome position: 10.61

GC content: 69.16

Gene sequence:

>1242_bases
ATGATCGGCAACGCGCCCTGGATCGCGCTTCCGGAGACCAAGGCGGTCATCGCCGCGCTGGAAGCGCGCGGCTATGCGGG
CTGCGCGCGATTCGTCGGCGGATGCGTGCGCAACACGCTGATGGGCAAGCCTGTCGACGACATCGACATCGCCACGACCC
TGACGCCCGATCTGGTCATCGACGCTTTGGAGCAGGCAGGCTTGCGCGCCATTCCGACCGGCGTCGACCACGGCACGATC
ACGGCGGTGTCGGGCGGTCGCCCCTACGAGATCACCACCCTGCGCAAGGACGTCTCGACCGACGGCCGCCGGGCGGTGGT
GGCCTTCACCCAGGACTGGAACCAGGACGCCGAGCGGCGCGACTTCCGCTTCAACGCGCTTTATGCGGATACGGACGGTC
GGGTCTATGATCCCACCGGCGAGGGGATCCGCGACGCCCATGAGGGCCGGGTGGTGTTCGTCGGCGATCCGATGACCCGC
ATCCGCGAGGACTATCTGCGTATCCTGCGCTTCTTCCGCTTCCAGGCCTGGTACGGCCGGGGCGAGCCGGACCAGAAGGG
CCTGGCGGCGTGCAAGGCGCTGAAGGGCATGGTCTCGGGCCGCGCGGCCGAGCGCACTCAGAAGGAGCTGATGAAGATGC
TGGCGGCGGACGATCCGCGTCCGGCCTTCCGTCTGATGGCCGCCACCAGCGTGCTGTCCTCGATCCTGCCGTCGGTGAAG
TCGCTGGCGCGCTTCGAGGCCCTGGTCGGCATCGAGACCGAGCAGCTGTTCGAGAACGACCCCGACCTGCGCCTGGCGGC
GCTGATTCCCGACGACCCCAAGGTCGCCGAGCAGCTGGCCGAGCGCCTGCGCCTGCCCAACAATGTCCGTGATCGCCTGG
TCGAGGCCGTCGGCAAGAACCCGCGCGTCGTCTCGTGGATGAGCCCGCGCGAGGCGCGCCGGGCGGTCTATGGCCTGGGG
ACCCGCACCTTCTGCGATCGGATCAAGCTGGCCTGGGCCGGCTCTGGCCGTGACTCCACCGCGCCCCAGTGGCGCGCCCT
GCTGGCCCTGGCCGAGACCTGGAACCCGCCGGCCTTCCCGCTGTCGGGCGAGGAGGTGATGAAGGCCGGCGTGCCCAAGG
GGCCGATGGTCGGCGAGGTCATGCGCGAGATCGAGCTCTGGTGGATCGACCAGGACTTCATCGAGGACAAGTTCAGCGCC
ATCGAGCGCCTGAAGGCCGTCGCCCAGGGCATGGTCTACTGA

Upstream 100 bases:

>100_bases
TTTCTTGTCTGGCGAAGCCGGGGTCTAGTGGCCTAACAATGTCGCCCCTTCGTTGGAGGGGATCGGACGCGTGAGTGGGA
GTCAGGCGTTGAGCAGTGAA

Downstream 100 bases:

>100_bases
TCGCCGTGGCGACGCGCGTCTTGAAGATCGGCCTGCTGGAAACGGGCGAGCCGCCGGGCCCGCTGAAGGCGACCTATGGC
GGCTATGGATCGATGTTCGA

Product: poly A polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MIGNAPWIALPETKAVIAALEARGYAGCARFVGGCVRNTLMGKPVDDIDIATTLTPDLVIDALEQAGLRAIPTGVDHGTI
TAVSGGRPYEITTLRKDVSTDGRRAVVAFTQDWNQDAERRDFRFNALYADTDGRVYDPTGEGIRDAHEGRVVFVGDPMTR
IREDYLRILRFFRFQAWYGRGEPDQKGLAACKALKGMVSGRAAERTQKELMKMLAADDPRPAFRLMAATSVLSSILPSVK
SLARFEALVGIETEQLFENDPDLRLAALIPDDPKVAEQLAERLRLPNNVRDRLVEAVGKNPRVVSWMSPREARRAVYGLG
TRTFCDRIKLAWAGSGRDSTAPQWRALLALAETWNPPAFPLSGEEVMKAGVPKGPMVGEVMREIELWWIDQDFIEDKFSA
IERLKAVAQGMVY

Sequences:

>Translated_413_residues
MIGNAPWIALPETKAVIAALEARGYAGCARFVGGCVRNTLMGKPVDDIDIATTLTPDLVIDALEQAGLRAIPTGVDHGTI
TAVSGGRPYEITTLRKDVSTDGRRAVVAFTQDWNQDAERRDFRFNALYADTDGRVYDPTGEGIRDAHEGRVVFVGDPMTR
IREDYLRILRFFRFQAWYGRGEPDQKGLAACKALKGMVSGRAAERTQKELMKMLAADDPRPAFRLMAATSVLSSILPSVK
SLARFEALVGIETEQLFENDPDLRLAALIPDDPKVAEQLAERLRLPNNVRDRLVEAVGKNPRVVSWMSPREARRAVYGLG
TRTFCDRIKLAWAGSGRDSTAPQWRALLALAETWNPPAFPLSGEEVMKAGVPKGPMVGEVMREIELWWIDQDFIEDKFSA
IERLKAVAQGMVY
>Mature_413_residues
MIGNAPWIALPETKAVIAALEARGYAGCARFVGGCVRNTLMGKPVDDIDIATTLTPDLVIDALEQAGLRAIPTGVDHGTI
TAVSGGRPYEITTLRKDVSTDGRRAVVAFTQDWNQDAERRDFRFNALYADTDGRVYDPTGEGIRDAHEGRVVFVGDPMTR
IREDYLRILRFFRFQAWYGRGEPDQKGLAACKALKGMVSGRAAERTQKELMKMLAADDPRPAFRLMAATSVLSSILPSVK
SLARFEALVGIETEQLFENDPDLRLAALIPDDPKVAEQLAERLRLPNNVRDRLVEAVGKNPRVVSWMSPREARRAVYGLG
TRTFCDRIKLAWAGSGRDSTAPQWRALLALAETWNPPAFPLSGEEVMKAGVPKGPMVGEVMREIELWWIDQDFIEDKFSA
IERLKAVAQGMVY

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=374, Percent_Identity=32.620320855615, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI87081691, Length=238, Percent_Identity=28.9915966386555, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789436, Length=305, Percent_Identity=26.5573770491803, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=392, Percent_Identity=33.4183673469388, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI281360112, Length=396, Percent_Identity=33.8383838383838, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21357337, Length=396, Percent_Identity=33.8383838383838, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45763; Mature: 45763

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGNAPWIALPETKAVIAALEARGYAGCARFVGGCVRNTLMGKPVDDIDIATTLTPDLVI
CCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHH
DALEQAGLRAIPTGVDHGTITAVSGGRPYEITTLRKDVSTDGRRAVVAFTQDWNQDAERR
HHHHHCCCEECCCCCCCCEEEEECCCCCEEEEEHHHHCCCCCCEEEEEEECCCCCCHHHH
DFRFNALYADTDGRVYDPTGEGIRDAHEGRVVFVGDPMTRIREDYLRILRFFRFQAWYGR
CCEEEEEEECCCCEEECCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
GEPDQKGLAACKALKGMVSGRAAERTQKELMKMLAADDPRPAFRLMAATSVLSSILPSVK
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SLARFEALVGIETEQLFENDPDLRLAALIPDDPKVAEQLAERLRLPNNVRDRLVEAVGKN
HHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
PRVVSWMSPREARRAVYGLGTRTFCDRIKLAWAGSGRDSTAPQWRALLALAETWNPPAFP
CCEEEECCHHHHHHHHHCCCHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
LSGEEVMKAGVPKGPMVGEVMREIELWWIDQDFIEDKFSAIERLKAVAQGMVY
CCHHHHHHCCCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIGNAPWIALPETKAVIAALEARGYAGCARFVGGCVRNTLMGKPVDDIDIATTLTPDLVI
CCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHH
DALEQAGLRAIPTGVDHGTITAVSGGRPYEITTLRKDVSTDGRRAVVAFTQDWNQDAERR
HHHHHCCCEECCCCCCCCEEEEECCCCCEEEEEHHHHCCCCCCEEEEEEECCCCCCHHHH
DFRFNALYADTDGRVYDPTGEGIRDAHEGRVVFVGDPMTRIREDYLRILRFFRFQAWYGR
CCEEEEEEECCCCEEECCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
GEPDQKGLAACKALKGMVSGRAAERTQKELMKMLAADDPRPAFRLMAATSVLSSILPSVK
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SLARFEALVGIETEQLFENDPDLRLAALIPDDPKVAEQLAERLRLPNNVRDRLVEAVGKN
HHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
PRVVSWMSPREARRAVYGLGTRTFCDRIKLAWAGSGRDSTAPQWRALLALAETWNPPAFP
CCEEEECCHHHHHHHHHCCCHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
LSGEEVMKAGVPKGPMVGEVMREIELWWIDQDFIEDKFSAIERLKAVAQGMVY
CCHHHHHHCCCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA