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The map label for this gene is nrdF

Identifier: 16124515

GI number: 16124515

Start: 273695

End: 274738

Strand: Direct

Name: nrdF

Synonym: CC_0260

Alternate gene names: 16124515

Gene position: 273695-274738 (Clockwise)

Preceding gene: 16124509

Following gene: 16124516

Centisome position: 6.81

GC content: 61.49

Gene sequence:

>1044_bases
ATGTCCGCTCCGTCATCGCTGATCCTGCCTGGCCTTATGACCCCTTCGGGCGGCTACAAGCCGTTCCGCTATCCGTGGGC
CTATGACTTCTGGAAGAAGCAGCAGCAGGTCCACTGGATGCCCGAAGAGGTGCCGCTGGGCGAGGACCTCAAGGACTGGG
CCGTCAAGCTGAACGACAAGGAACGGAACCTGCTGACGCAGATCTTCCGCTTCTTCACCCAGTCCGACGTCGAGGTGCAG
GACAACTACATGGAGCGCTACGGTCGGGTGTTCAAACCGACCGAAGTGAAGATGATGCTGGCCTCGTTCGCGAACATGGA
GACGATCCATATCGCGGCCTACGCCCTGCTCCTCGAGACCATCGGCATGCCCGAGACCGAGTTCTCGGCGTTCATGGAAT
ACGAGGCCATGAAGGCCAAGCACGACTACATGCAGACCTTCGGTGTCGACTCGAACGCCGACATCTGCCGCACCCTGGCT
ATGTTCGGCGGCTTCACCGAAGGCCTGCAGCTGTTCGCCTCGTTCGCGATGCTGATGAACTTCCCGCGCTTCAACAAGAT
GAAGGGCATGGGCCAGATCGTCTCGTGGTCGATCCGCGACGAGAGCCTGCACTGCGACGGCATCATCAAGCTGTACCACG
CCTTCAACAAGGAAACCGGCGCGGTGACCAAGGCCGTGGCCGACGACATCGTCGACTGCTGCAAGACCGTGGTCGGCATG
GAAGACAACTTCATCGACCTGGCCTTCGAGGCCGGCGACATCCAGGGCATGACGCCTGACGACATCAAGGCCTATATCCG
CTTCGTCGCCGACTGGCGCCTGCGCCAGCTACACCTGCCCGAAGTGTACGGCGTGAAGGAAAACCCGCTGCCCTGGCTGC
AGTCGCTGCTGTCGGGCGTGGAGCACGCCAACTTCTTCGAAGCCCGCGCGACCGAGTATTCCAAGGCCGCCACCAAGGGT
CAGTGGCACGGCTCGGACGGCGTCTGGACCAGCTTCGACGAGATGATGAAGAAGCGCTCGGCCGAAACGATCCCGGCGGA
ATGA

Upstream 100 bases:

>100_bases
TCTTGTGGGGGTGCGACGCTGGCGCCACTAGATGAACCGAGCGGCTTTGGTAGGGTAAAGCACCTTACCGCGTCCGCTCG
ATTCTGCCCGAGGCCCTGTC

Downstream 100 bases:

>100_bases
TGTTGGGCGTCTTCTCCCCCTTGCGGGAGAAGAGCTTGCCCCGGACTTGATGCGGGGTGTCAGCGAAGCTGACGGATGAG
GGGTTTCCCGGCGTCGCCCG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSAPSSLILPGLMTPSGGYKPFRYPWAYDFWKKQQQVHWMPEEVPLGEDLKDWAVKLNDKERNLLTQIFRFFTQSDVEVQ
DNYMERYGRVFKPTEVKMMLASFANMETIHIAAYALLLETIGMPETEFSAFMEYEAMKAKHDYMQTFGVDSNADICRTLA
MFGGFTEGLQLFASFAMLMNFPRFNKMKGMGQIVSWSIRDESLHCDGIIKLYHAFNKETGAVTKAVADDIVDCCKTVVGM
EDNFIDLAFEAGDIQGMTPDDIKAYIRFVADWRLRQLHLPEVYGVKENPLPWLQSLLSGVEHANFFEARATEYSKAATKG
QWHGSDGVWTSFDEMMKKRSAETIPAE

Sequences:

>Translated_347_residues
MSAPSSLILPGLMTPSGGYKPFRYPWAYDFWKKQQQVHWMPEEVPLGEDLKDWAVKLNDKERNLLTQIFRFFTQSDVEVQ
DNYMERYGRVFKPTEVKMMLASFANMETIHIAAYALLLETIGMPETEFSAFMEYEAMKAKHDYMQTFGVDSNADICRTLA
MFGGFTEGLQLFASFAMLMNFPRFNKMKGMGQIVSWSIRDESLHCDGIIKLYHAFNKETGAVTKAVADDIVDCCKTVVGM
EDNFIDLAFEAGDIQGMTPDDIKAYIRFVADWRLRQLHLPEVYGVKENPLPWLQSLLSGVEHANFFEARATEYSKAATKG
QWHGSDGVWTSFDEMMKKRSAETIPAE
>Mature_346_residues
SAPSSLILPGLMTPSGGYKPFRYPWAYDFWKKQQQVHWMPEEVPLGEDLKDWAVKLNDKERNLLTQIFRFFTQSDVEVQD
NYMERYGRVFKPTEVKMMLASFANMETIHIAAYALLLETIGMPETEFSAFMEYEAMKAKHDYMQTFGVDSNADICRTLAM
FGGFTEGLQLFASFAMLMNFPRFNKMKGMGQIVSWSIRDESLHCDGIIKLYHAFNKETGAVTKAVADDIVDCCKTVVGME
DNFIDLAFEAGDIQGMTPDDIKAYIRFVADWRLRQLHLPEVYGVKENPLPWLQSLLSGVEHANFFEARATEYSKAATKGQ
WHGSDGVWTSFDEMMKKRSAETIPAE

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=316, Percent_Identity=30.0632911392405, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI289177074, Length=316, Percent_Identity=30.379746835443, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI260064013, Length=300, Percent_Identity=29, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4557845, Length=300, Percent_Identity=29, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI289177076, Length=295, Percent_Identity=30.1694915254237, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17551968, Length=300, Percent_Identity=31.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI193206379, Length=285, Percent_Identity=25.6140350877193, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322434, Length=320, Percent_Identity=27.5, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI18079279, Length=299, Percent_Identity=29.0969899665552, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 39699; Mature: 39568

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSSLILPGLMTPSGGYKPFRYPWAYDFWKKQQQVHWMPEEVPLGEDLKDWAVKLNDK
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCH
ERNLLTQIFRFFTQSDVEVQDNYMERYGRVFKPTEVKMMLASFANMETIHIAAYALLLET
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
IGMPETEFSAFMEYEAMKAKHDYMQTFGVDSNADICRTLAMFGGFTEGLQLFASFAMLMN
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
FPRFNKMKGMGQIVSWSIRDESLHCDGIIKLYHAFNKETGAVTKAVADDIVDCCKTVVGM
CCCHHHHCCCCHHEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
EDNFIDLAFEAGDIQGMTPDDIKAYIRFVADWRLRQLHLPEVYGVKENPLPWLQSLLSGV
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHH
EHANFFEARATEYSKAATKGQWHGSDGVWTSFDEMMKKRSAETIPAE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAPSSLILPGLMTPSGGYKPFRYPWAYDFWKKQQQVHWMPEEVPLGEDLKDWAVKLNDK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCH
ERNLLTQIFRFFTQSDVEVQDNYMERYGRVFKPTEVKMMLASFANMETIHIAAYALLLET
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
IGMPETEFSAFMEYEAMKAKHDYMQTFGVDSNADICRTLAMFGGFTEGLQLFASFAMLMN
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
FPRFNKMKGMGQIVSWSIRDESLHCDGIIKLYHAFNKETGAVTKAVADDIVDCCKTVVGM
CCCHHHHCCCCHHEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
EDNFIDLAFEAGDIQGMTPDDIKAYIRFVADWRLRQLHLPEVYGVKENPLPWLQSLLSGV
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHH
EHANFFEARATEYSKAATKGQWHGSDGVWTSFDEMMKKRSAETIPAE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]