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The map label for this gene is arnB [H]

Identifier: 16124489

GI number: 16124489

Start: 249068

End: 250276

Strand: Direct

Name: arnB [H]

Synonym: CC_0234

Alternate gene names: 16124489

Gene position: 249068-250276 (Clockwise)

Preceding gene: 16124488

Following gene: 16124492

Centisome position: 6.2

GC content: 69.81

Gene sequence:

>1209_bases
TTGGCTCAGCCCCGAGGGTCTGCTGGCCATGCTGGAGGAGAAGGCCACGTGACGGGCGGCTTTCTCCCCTACGGCCGGCA
GACGATCGAGGAGGACGATATCGCCGCCGTGGCGGAGGCCCTGCGTGGCGATTTCCTGACCACCGGTCCGACGGTCGAGG
CCTTCGAGACCGCCTTCGCCGCCAAGGTCGGCGCCGATCACGCGATCGCCGTTTCCAACGGTACGGCGACCCTGCATCTG
GCGATGATGGCGCTGGGCATAGGAGAGGGGGATGTCTGCGTCGCCCCGTCCGTCACCTTTCTGGCCACCGCCAATTGCGC
CCGGTATGTGGGCGCCGAGGTCGTCTTCGCCGACGTCGATCCCGACAGTGGCCTGATGACGCCCGACACGCTGGCTCGGG
CCCTGGCCGGCGCGCGCGACAAGCGCGTCAAGGCGGTGCTGCCGGTGCACCTGCGTGGCGACGTCTGCGATCTGCCCGCG
CTCAAGGCCATGGCCAGCGCCTCGGGCGCGGTGCTCGTCGAGGACGCCCCGCACGCCCTGGGCTCGATCGCCACCTTCGA
CGGTGTCGCCCACCCGGTCGGCGACGGCGCCTATTCCAGCTTCGCCAGCTTCTCGTTTCACCCGGTCAAGACGCTCGCCA
CGGGTGAGGGCGGCATGCTGACCACCAACGATCCCGCCCTGGCCGCCAAGGCGCGCTTGCTGCGCTCACATGGCATGGTG
CGCCAGCCGGGCGGCGACCCGTGGTGGTACGAGATGCCGGAGCTGGGCTTCAACTACCGCATCCCCGACGTGCTCTGCGC
GCTGGGCCTGTCGCAGCTTGCGAAACTGGACCGGTTCGTTGCGCGACGTCGCGACCTGACGGCGCTTTATGCGCGCCTGC
TGGCGGAGCGCGCGCCGCGCGCGCGGCTGGCGACCAGCCCCGACCATTCGGACGCCGCGCTGCACCTGTTGACCGTGCTG
ATCGATTTCGAGGCCGAGGGAATCTCGCGGCGCACCGTTGTCGAGAGCCTCAAGACCCAGGGCGTCGGGACCCAGGTCCA
CTACATTCCGGTCCATCGCCAGCCCTACTACGCCCAACGCTATGGCGTCGCGGACCTGCCCGGCGCGGACGCCTGGTACG
CGCGCTGCCTGACGCTGCCGCTCTATCCGGCCATGACCAACGGCGACGTCGAGCGCGTGGTGGGCGCCCTGGCCACAGTT
CTCGGTTAA

Upstream 100 bases:

>100_bases
AGCCCAACTTCGCCGAGTTCGGTCGTGAGCCCTACGCCGCCTCCGACGGCGCCAAGCCGGTCGCCGAGGATTTCTCGTAC
AGCAGCGACAACAACCACGA

Downstream 100 bases:

>100_bases
CCTCGTGACTTCGCGGGGCGGCCCTTGAAGTAGGCCAACGGAAACACCGTGCCGAAGCAGACGGTCATGGCGCCCAAAAC
CAGGACGAACGGCGGGACGC

Product: flagellin modification protein FlmB

Products: NA

Alternate protein names: UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase; UDP-Ara4O aminotransferase; UDP-4-amino-4-deoxy-L-arabinose aminotransferase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAQPRGSAGHAGGEGHVTGGFLPYGRQTIEEDDIAAVAEALRGDFLTTGPTVEAFETAFAAKVGADHAIAVSNGTATLHL
AMMALGIGEGDVCVAPSVTFLATANCARYVGAEVVFADVDPDSGLMTPDTLARALAGARDKRVKAVLPVHLRGDVCDLPA
LKAMASASGAVLVEDAPHALGSIATFDGVAHPVGDGAYSSFASFSFHPVKTLATGEGGMLTTNDPALAAKARLLRSHGMV
RQPGGDPWWYEMPELGFNYRIPDVLCALGLSQLAKLDRFVARRRDLTALYARLLAERAPRARLATSPDHSDAALHLLTVL
IDFEAEGISRRTVVESLKTQGVGTQVHYIPVHRQPYYAQRYGVADLPGADAWYARCLTLPLYPAMTNGDVERVVGALATV
LG

Sequences:

>Translated_402_residues
MAQPRGSAGHAGGEGHVTGGFLPYGRQTIEEDDIAAVAEALRGDFLTTGPTVEAFETAFAAKVGADHAIAVSNGTATLHL
AMMALGIGEGDVCVAPSVTFLATANCARYVGAEVVFADVDPDSGLMTPDTLARALAGARDKRVKAVLPVHLRGDVCDLPA
LKAMASASGAVLVEDAPHALGSIATFDGVAHPVGDGAYSSFASFSFHPVKTLATGEGGMLTTNDPALAAKARLLRSHGMV
RQPGGDPWWYEMPELGFNYRIPDVLCALGLSQLAKLDRFVARRRDLTALYARLLAERAPRARLATSPDHSDAALHLLTVL
IDFEAEGISRRTVVESLKTQGVGTQVHYIPVHRQPYYAQRYGVADLPGADAWYARCLTLPLYPAMTNGDVERVVGALATV
LG
>Mature_401_residues
AQPRGSAGHAGGEGHVTGGFLPYGRQTIEEDDIAAVAEALRGDFLTTGPTVEAFETAFAAKVGADHAIAVSNGTATLHLA
MMALGIGEGDVCVAPSVTFLATANCARYVGAEVVFADVDPDSGLMTPDTLARALAGARDKRVKAVLPVHLRGDVCDLPAL
KAMASASGAVLVEDAPHALGSIATFDGVAHPVGDGAYSSFASFSFHPVKTLATGEGGMLTTNDPALAAKARLLRSHGMVR
QPGGDPWWYEMPELGFNYRIPDVLCALGLSQLAKLDRFVARRRDLTALYARLLAERAPRARLATSPDHSDAALHLLTVLI
DFEAEGISRRTVVESLKTQGVGTQVHYIPVHRQPYYAQRYGVADLPGADAWYARCLTLPLYPAMTNGDVERVVGALATVL
G

Specific function: Catalyzes the conversion of UDP-4-keto-arabinose (UDP- Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family. ArnB subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=387, Percent_Identity=35.4005167958656, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI2367285, Length=346, Percent_Identity=30.3468208092486, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022850
- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: =2.6.1.87 [H]

Molecular weight: Translated: 42325; Mature: 42193

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPRGSAGHAGGEGHVTGGFLPYGRQTIEEDDIAAVAEALRGDFLTTGPTVEAFETAFA
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AKVGADHAIAVSNGTATLHLAMMALGIGEGDVCVAPSVTFLATANCARYVGAEVVFADVD
HHCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHCCCEEEEEECC
PDSGLMTPDTLARALAGARDKRVKAVLPVHLRGDVCDLPALKAMASASGAVLVEDAPHAL
CCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHH
GSIATFDGVAHPVGDGAYSSFASFSFHPVKTLATGEGGMLTTNDPALAAKARLLRSHGMV
HHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHCCCE
RQPGGDPWWYEMPELGFNYRIPDVLCALGLSQLAKLDRFVARRRDLTALYARLLAERAPR
ECCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARLATSPDHSDAALHLLTVLIDFEAEGISRRTVVESLKTQGVGTQVHYIPVHRQPYYAQR
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
YGVADLPGADAWYARCLTLPLYPAMTNGDVERVVGALATVLG
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AQPRGSAGHAGGEGHVTGGFLPYGRQTIEEDDIAAVAEALRGDFLTTGPTVEAFETAFA
CCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AKVGADHAIAVSNGTATLHLAMMALGIGEGDVCVAPSVTFLATANCARYVGAEVVFADVD
HHCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHCCCEEEEEECC
PDSGLMTPDTLARALAGARDKRVKAVLPVHLRGDVCDLPALKAMASASGAVLVEDAPHAL
CCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHH
GSIATFDGVAHPVGDGAYSSFASFSFHPVKTLATGEGGMLTTNDPALAAKARLLRSHGMV
HHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHCCCE
RQPGGDPWWYEMPELGFNYRIPDVLCALGLSQLAKLDRFVARRRDLTALYARLLAERAPR
ECCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARLATSPDHSDAALHLLTVLIDFEAEGISRRTVVESLKTQGVGTQVHYIPVHRQPYYAQR
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
YGVADLPGADAWYARCLTLPLYPAMTNGDVERVVGALATVLG
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA