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The map label for this gene is dnaN

Identifier: 16124411

GI number: 16124411

Start: 163413

End: 164531

Strand: Direct

Name: dnaN

Synonym: CC_0156

Alternate gene names: 16124411

Gene position: 163413-164531 (Clockwise)

Preceding gene: 16124409

Following gene: 16124412

Centisome position: 4.07

GC content: 67.47

Gene sequence:

>1119_bases
ATGAAGCTTACGATCGAACGGGCGGCGCTCCTGAAGGCGCTGGGGCATGTGCAGAGCGTCGTCGAGCGCCGCAACACCAT
CCCGATCCTGTCGAACATCCTGCTGTCGGCTGAAGGTGACCGGCTGTCGTTCTCGGCCACGGACCTGGACATGGAGATCA
TCGACGAGGGCTTCGCCCAGATCGACGTTCCCGGCCAGATCACCGCGCCGGCCCACACCCTCTATGAGATCGTCCGCAAG
CTGCCCGACGGCGCGGACGTGTCGCTGAGCTTCAGCGGCGATGATCCGCGCCTGGTCATCCAGGCCGGCCGCTCGCGCTT
CAACCTGCCGGTGCTGCCGGCCGGCGACTTCCCGGTGATGAGCTCGGACGGCCTGTCCAGCCGCATCGCGGTCGACACCA
ACGAACTGATCCGCCTGATCGACAAGACCCGGTTCGCCATCTCGACCGAAGAGACGCGCTACTACCTCAACGGCCTCTAT
GTGCACACGGTCAATGAGGGCGGCGAGACCAAGCTTCGCGCCGTCGCCACCGACGGCCACCGTCTGGCCCTGGCCGAGAT
GCCCGCGCCCGAGGGCGCGGTCGGCATCCCGGGCGTGATCGTGCCGCGCAAGACCATCGCCGAAGCCCGTCGCCTGATGG
AATCGGCCGGCGAGACGGTCGACCTGCAGGTCTCCCCGCAGAAGGTCCGCTTCGAGTTCGGCGCGGCCGCCCTGACCTCC
AAGGTCATCGACGGCGCCTTCCCCGACTACATGCGGGTGATCCCGCGCGACAACGCCAAGATCCTGACCCTGGACAACGA
TCTCTTCGCCAAGGCCGTGGACCGGGTGGCCACCATCTCGGCCGAGAAGAGCCGCTCGGTGAAGCTGGCCGTCGAGCCTG
GCCGCATCACCCTGACCGTCCGCAACATGGAAGCCGGCCAGGCCGTCGAAGAGGTCGAGGTCGACTATGACGGCGAGCCC
TTCGAGATCGGCTTCAACGCCCGCTACCTGCTGGACGTCTGCGGTCAGATCGCCGGTCCGCAGGCGGAGTTCCGCTTCGC
CGACCCGGCCAGCCCGACCCTGGTGGTCGATCCCGTCGATCCGGGCGTGAAGTACGTGCTGATGCCGCTGCGGGTCTGA

Upstream 100 bases:

>100_bases
CGCGCGCGTCTTTTCGCTAATGTCGGCGGTCCCTTCTCCACGGGTTCGCGCGGGGCTGATTCTCCCCGGCGGCGGCTGAA
CCCGACATCTGGACCGGACT

Downstream 100 bases:

>100_bases
TCCGCAGCCGGGTCATCGGACCATTAAGAAACGGACGCCCTCGGGCGTCCGTTTTTGCTTTCAGGGTTCGCCGGGCTGCT
CTCCAAGGCTCAAGCTGTTG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKLTIERAALLKALGHVQSVVERRNTIPILSNILLSAEGDRLSFSATDLDMEIIDEGFAQIDVPGQITAPAHTLYEIVRK
LPDGADVSLSFSGDDPRLVIQAGRSRFNLPVLPAGDFPVMSSDGLSSRIAVDTNELIRLIDKTRFAISTEETRYYLNGLY
VHTVNEGGETKLRAVATDGHRLALAEMPAPEGAVGIPGVIVPRKTIAEARRLMESAGETVDLQVSPQKVRFEFGAAALTS
KVIDGAFPDYMRVIPRDNAKILTLDNDLFAKAVDRVATISAEKSRSVKLAVEPGRITLTVRNMEAGQAVEEVEVDYDGEP
FEIGFNARYLLDVCGQIAGPQAEFRFADPASPTLVVDPVDPGVKYVLMPLRV

Sequences:

>Translated_372_residues
MKLTIERAALLKALGHVQSVVERRNTIPILSNILLSAEGDRLSFSATDLDMEIIDEGFAQIDVPGQITAPAHTLYEIVRK
LPDGADVSLSFSGDDPRLVIQAGRSRFNLPVLPAGDFPVMSSDGLSSRIAVDTNELIRLIDKTRFAISTEETRYYLNGLY
VHTVNEGGETKLRAVATDGHRLALAEMPAPEGAVGIPGVIVPRKTIAEARRLMESAGETVDLQVSPQKVRFEFGAAALTS
KVIDGAFPDYMRVIPRDNAKILTLDNDLFAKAVDRVATISAEKSRSVKLAVEPGRITLTVRNMEAGQAVEEVEVDYDGEP
FEIGFNARYLLDVCGQIAGPQAEFRFADPASPTLVVDPVDPGVKYVLMPLRV
>Mature_372_residues
MKLTIERAALLKALGHVQSVVERRNTIPILSNILLSAEGDRLSFSATDLDMEIIDEGFAQIDVPGQITAPAHTLYEIVRK
LPDGADVSLSFSGDDPRLVIQAGRSRFNLPVLPAGDFPVMSSDGLSSRIAVDTNELIRLIDKTRFAISTEETRYYLNGLY
VHTVNEGGETKLRAVATDGHRLALAEMPAPEGAVGIPGVIVPRKTIAEARRLMESAGETVDLQVSPQKVRFEFGAAALTS
KVIDGAFPDYMRVIPRDNAKILTLDNDLFAKAVDRVATISAEKSRSVKLAVEPGRITLTVRNMEAGQAVEEVEVDYDGEP
FEIGFNARYLLDVCGQIAGPQAEFRFADPASPTLVVDPVDPGVKYVLMPLRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=37.8016085790885, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_CAUCN (B8GXP6)

Other databases:

- EMBL:   U37793
- EMBL:   CP001340
- EMBL:   U00591
- PIR:   A49347
- RefSeq:   NP_418975.1
- RefSeq:   YP_002515530.1
- ProteinModelPortal:   B8GXP6
- SMR:   B8GXP6
- GeneID:   7332409
- GeneID:   944215
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_00155
- HOGENOM:   HBG586514
- OMA:   DYNRVIP
- ProtClustDB:   PRK05643
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 40426; Mature: 40426

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTIERAALLKALGHVQSVVERRNTIPILSNILLSAEGDRLSFSATDLDMEIIDEGFAQ
CEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEECCCCHHHHHCCCEE
IDVPGQITAPAHTLYEIVRKLPDGADVSLSFSGDDPRLVIQAGRSRFNLPVLPAGDFPVM
ECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCEEECCCCCCCC
SSDGLSSRIAVDTNELIRLIDKTRFAISTEETRYYLNGLYVHTVNEGGETKLRAVATDGH
CCCCCCCEEEECHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLALAEMPAPEGAVGIPGVIVPRKTIAEARRLMESAGETVDLQVSPQKVRFEFGAAALTS
EEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEEECCHHEEEEHHHHHHHH
KVIDGAFPDYMRVIPRDNAKILTLDNDLFAKAVDRVATISAEKSRSVKLAVEPGRITLTV
HHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHEECCCCCCCEEEEEECCEEEEEE
RNMEAGQAVEEVEVDYDGEPFEIGFNARYLLDVCGQIAGPQAEFRFADPASPTLVVDPVD
ECCCCCCCHHHEECCCCCCCEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEECCCC
PGVKYVLMPLRV
CCCEEEEEEECC
>Mature Secondary Structure
MKLTIERAALLKALGHVQSVVERRNTIPILSNILLSAEGDRLSFSATDLDMEIIDEGFAQ
CEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEECCCCHHHHHCCCEE
IDVPGQITAPAHTLYEIVRKLPDGADVSLSFSGDDPRLVIQAGRSRFNLPVLPAGDFPVM
ECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCEEECCCCCCCC
SSDGLSSRIAVDTNELIRLIDKTRFAISTEETRYYLNGLYVHTVNEGGETKLRAVATDGH
CCCCCCCEEEECHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLALAEMPAPEGAVGIPGVIVPRKTIAEARRLMESAGETVDLQVSPQKVRFEFGAAALTS
EEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEEECCHHEEEEHHHHHHHH
KVIDGAFPDYMRVIPRDNAKILTLDNDLFAKAVDRVATISAEKSRSVKLAVEPGRITLTV
HHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHEECCCCCCCEEEEEECCEEEEEE
RNMEAGQAVEEVEVDYDGEPFEIGFNARYLLDVCGQIAGPQAEFRFADPASPTLVVDPVD
ECCCCCCCHHHEECCCCCCCEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEECCCC
PGVKYVLMPLRV
CCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640