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The map label for this gene is grpE

Identifier: 16124409

GI number: 16124409

Start: 162439

End: 163035

Strand: Direct

Name: grpE

Synonym: CC_0154

Alternate gene names: 16124409

Gene position: 162439-163035 (Clockwise)

Preceding gene: 16124408

Following gene: 16124411

Centisome position: 4.04

GC content: 70.18

Gene sequence:

>597_bases
ATGCCGTTCGAAGCCGATGACGCCGCGCAGGAGATCGAGGCGCTGAAGCTGGAAGTGGCGCAACTGAAGGAGCAGGCGCT
CCGCTACGCCGCCGAGGCCGAGAACACCAAGCGCCGCGCCGAGCGCGAGATGAACGATGCGCGCGCCTATGCGATCCAGA
AGTTCGCCCGCGACCTCCTGGGCGCCGCCGACAATCTGGGCCGCGCCACCGCGCACAGCCCCAAGGACTCGACCGACCCG
GCGGTGAAGAACTTCATCATCGGCGTCGAGATGACCGAGAAGGAGCTGCAGAGCGCCTTCGAACGCAACGGCCTGAAGAA
GATCGATCCGGCCAAGGGCGACAAGTTCGACCCGCACCTGCACCAGGCCGTCACCGAGCAGCCCTCGACCGAGGTCGCCG
CCGGCGGCGTGCTGATGGTGATGCAGGCCGGCTATGAGCTGATGGGCCGCCTGGTTCGTCCCGCCATGGTCGCCGTGGCG
GCCAAGGGCTCGACGGGGCCGGCCTCGCCGGATGCGCCCGCAGCCTCGGCCAACCCCTACGCCGGCGCGGCCGCCGAGGG
CGACAGCACGGGCGGCGCGTTCGACGCCAAGGCCTGA

Upstream 100 bases:

>100_bases
GGTCATTCCGTTGGTGGACTATACCGCCCGGGTGCTCGGGCGGATGATGGATGGATAAGGACTTTACCCAATGACCGACG
AGCAAACGCCGGCGGAAGAG

Downstream 100 bases:

>100_bases
TCGCCGGATCGGTCGCGCGCTCGCTGGTCGGCGCGCGACCACAGCCGCTCATGGATGTTGAAGGCCACGGTCTGGACCAT
CGGTTCGACCAGACCGACCG

Product: grpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MPFEADDAAQEIEALKLEVAQLKEQALRYAAEAENTKRRAEREMNDARAYAIQKFARDLLGAADNLGRATAHSPKDSTDP
AVKNFIIGVEMTEKELQSAFERNGLKKIDPAKGDKFDPHLHQAVTEQPSTEVAAGGVLMVMQAGYELMGRLVRPAMVAVA
AKGSTGPASPDAPAASANPYAGAAAEGDSTGGAFDAKA

Sequences:

>Translated_198_residues
MPFEADDAAQEIEALKLEVAQLKEQALRYAAEAENTKRRAEREMNDARAYAIQKFARDLLGAADNLGRATAHSPKDSTDP
AVKNFIIGVEMTEKELQSAFERNGLKKIDPAKGDKFDPHLHQAVTEQPSTEVAAGGVLMVMQAGYELMGRLVRPAMVAVA
AKGSTGPASPDAPAASANPYAGAAAEGDSTGGAFDAKA
>Mature_197_residues
PFEADDAAQEIEALKLEVAQLKEQALRYAAEAENTKRRAEREMNDARAYAIQKFARDLLGAADNLGRATAHSPKDSTDPA
VKNFIIGVEMTEKELQSAFERNGLKKIDPAKGDKFDPHLHQAVTEQPSTEVAAGGVLMVMQAGYELMGRLVRPAMVAVAA
KGSTGPASPDAPAASANPYAGAAAEGDSTGGAFDAKA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=154, Percent_Identity=40.2597402597403, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI40255109, Length=165, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1788967, Length=143, Percent_Identity=37.7622377622378, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552458, Length=162, Percent_Identity=36.4197530864198, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=145, Percent_Identity=33.7931034482759, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653432, Length=159, Percent_Identity=37.1069182389937, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CAUCN (B8GXP4)

Other databases:

- EMBL:   U33324
- EMBL:   CP001340
- RefSeq:   YP_002515528.1
- GeneID:   7332407
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_00153
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14155
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20885; Mature: 20753

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFEADDAAQEIEALKLEVAQLKEQALRYAAEAENTKRRAEREMNDARAYAIQKFARDLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAADNLGRATAHSPKDSTDPAVKNFIIGVEMTEKELQSAFERNGLKKIDPAKGDKFDPHL
HHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHH
HQAVTEQPSTEVAAGGVLMVMQAGYELMGRLVRPAMVAVAAKGSTGPASPDAPAASANPY
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AGAAAEGDSTGGAFDAKA
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PFEADDAAQEIEALKLEVAQLKEQALRYAAEAENTKRRAEREMNDARAYAIQKFARDLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAADNLGRATAHSPKDSTDPAVKNFIIGVEMTEKELQSAFERNGLKKIDPAKGDKFDPHL
HHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHH
HQAVTEQPSTEVAAGGVLMVMQAGYELMGRLVRPAMVAVAAKGSTGPASPDAPAASANPY
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AGAAAEGDSTGGAFDAKA
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606155