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The map label for this gene is polB2

Identifier: 16120259

GI number: 16120259

Start: 289084

End: 291252

Strand: Direct

Name: polB2

Synonym: VNG6362G

Alternate gene names: 16120259

Gene position: 289084-291252 (Clockwise)

Preceding gene: 16120258

Following gene: 16120267

Centisome position: 79.11

GC content: 64.41

Gene sequence:

>2169_bases
ATGCCGTTCAGTATCGATTTCCTGGACGACGGCCGCGTCCTGGAGTGGGAGGCGACCGCTGACGGTGCCGTCGCAACAGA
ACGGGATAACTACACACCGCGATTCTACGTCGCCGCTCGCGACCCTGATGCCGACCTCGACCTCACGACACTCCAGTCGG
TGTACGACCAGCACCCGGATGTCGTCGCGACCGAGATGGTTGCGCGACGCCCCGGCTTTCGACGAGACGAGGAGGCCGTT
CTCGCAGTCGACGTTGCCCACATCGATCGCGTCACTCCACTCGCCCGGCAGGCGCGTCAGCTGTCGGCCTATCCAGTCGG
GGATCTCGCCTGTTTCAACGTGGACTTTTCGCGAGAGTTCCGATACTGTCTAGAGACCGGCGCCGATCCGACGCCAGCGA
GCGAGCTGTCGACGCTCCGGCTCAGTGTCCCGGTGACCGAAACGAGCAACGACATCTACGAGGAACTGTCCGTCGCCGGC
GACGCCGTCACCGGCTCGTCGACGGATATCCTGGCCGCCGTCCAGGGGGCGCTCGAAGCACACGATCCGGACGTCTTGGT
CTGCTCGACGAGCGAGATCGTCCCGACCCTTCACGAGATGGCGACGGACGCCGGCGTCGACGACTTCTCGCTGAGTCGGT
GGCCGGACGTCGACTACCAGCAGCTCGCGAGCCGGTCGACGTACTCGAGCTACGGCCGCGTCGGCCACTCGCCGGCGCGG
TACAACGTGCCCGGCCGGGCGATTATCGACGAGTCGAACACGTTCTTCTACGGGGAGACGAACCTCGACGGCGTCCTCGA
CCTCGTGTCGCGCTCGAAAAATCCCGTTCAGGAGCTCGCGTGGGCATCGATCGGGAACGTTCTCACGGCAATCCAGATCT
GCGAGGCCAACGACCGCGGCGTCCTCGTCCCATGGAACTCCTGGCGCCACGAGTTCTACAAGCCGATGGGGACACTCCAC
GACGCCGACCGCGGCGGGTTCATCTTCGCCCCCGAGGTCGGCCTCCACGAGAACGTTCACGAACTCGACTTCTCCTCGTT
GTATCCGAACATCATCTGTACCCGGAACGTCTCGCCGGACGTCATCCGGTGTGACTGCCACAGCGACCGCGACGACGTCC
CCGGTCTCGGATACTCGATCTGCGACGACCGGGGCTACCTCGTTGACGTGCTACAGCCGATCATCGACGCGCGCGACGAG
ATCAAGGCGGCCATCCGTCGCGAGACGGATCGAGACGACCCCGATGAGGACCGACTGGCAGAACTCGAGGGACGGTCGGG
AGCGCTGAAGTGGATCCTCGTCGCCTGCTTCGGCTATCAAGGGTTCAGCAACGCGAAGTTCGGCCGCATCGAGTGCCACG
AAGCGATCAACGCGTTCGCTCGCGAGATTCTGCTGATGGCGAAACAGCGACTGGAAGCCGGCGGCTGGCGGGTCGTCCAC
GGCATCGTCGACTCCATCTGGGTGACCCCGGACCCTGACGTCGACGAGGAGGACCGCGAAGACCTCCAGACGCTCGCGAC
GGAGATCACGGAACGTGTCGAGATTCGGCTCGAACACGAGGCGCACTACGACTGGGTGGCGTTCGTGCCGCAGCGCGAGA
GCGACGCCGGCGCGCTGACGAAGTACTTCGGGAAGGTCGCCGGCGACGACGACTTCAAGGTCAGAGGTATCGAAGCCCGG
CAGCGCTCGACCCCGCCGTTCATTGAGGACGTCCAGCGGGACTGTCTTGACCGGCTCGATGCCACGCGGTCACCGGACGC
GGTGCTTGGACGGCTCGAACGAGCAATCGACGAACTGCAGGCGGGCAACGTGGCAGTGGAGCGGCTCGTCGAACGGAACC
GTGTCTCGAAGCCCCTGGAGGGCTACTCACAGAATACCCAGAACGTGGCCGCCTTGAAGCGAGCCCGCGAGCAGGACCTA
GCAGTCCACCCAGGCCAGGATATCGAGTACGTGGTCGTCGACGACGAGAAATCCTCACGAGACCGGGTCGCCCTCGCCCA
CGAAGCGATCGAGACCTACGACACCTCGTACTACGAAACGCAGCTGGTCAGAGCGGTCGAGAGCGTGCTTTCACCGCTCG
GGTGGGATCGGTCCAACATTCATCGAGAGCTCTCCGGTGAGAGGGACGCCGTTTTGACCTCGTTCGGAGCCTCCAACGAA
CTGTCGTAG

Upstream 100 bases:

>100_bases
CGCCGTGACCGAGATGGTTGCGAGAGAGACCATCGTTCCCTGAAATCCGGAAGTGACACGCCATCGATTATCCGGAACCC
CACTGTTGGTTCTGTAGGGA

Downstream 100 bases:

>100_bases
TGGACCCCACCCCTGGTTTCCGTCGTTTCGATCCAACCCCACGTTTCCGACGTAATTTTCGACCCCCTCGTTTCCGTCGT
TTCCTCACGCCCCACCCCAG

Product: DNA polymerase B2

Products: NA

Alternate protein names: Pfu polymerase [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MPFSIDFLDDGRVLEWEATADGAVATERDNYTPRFYVAARDPDADLDLTTLQSVYDQHPDVVATEMVARRPGFRRDEEAV
LAVDVAHIDRVTPLARQARQLSAYPVGDLACFNVDFSREFRYCLETGADPTPASELSTLRLSVPVTETSNDIYEELSVAG
DAVTGSSTDILAAVQGALEAHDPDVLVCSTSEIVPTLHEMATDAGVDDFSLSRWPDVDYQQLASRSTYSSYGRVGHSPAR
YNVPGRAIIDESNTFFYGETNLDGVLDLVSRSKNPVQELAWASIGNVLTAIQICEANDRGVLVPWNSWRHEFYKPMGTLH
DADRGGFIFAPEVGLHENVHELDFSSLYPNIICTRNVSPDVIRCDCHSDRDDVPGLGYSICDDRGYLVDVLQPIIDARDE
IKAAIRRETDRDDPDEDRLAELEGRSGALKWILVACFGYQGFSNAKFGRIECHEAINAFAREILLMAKQRLEAGGWRVVH
GIVDSIWVTPDPDVDEEDREDLQTLATEITERVEIRLEHEAHYDWVAFVPQRESDAGALTKYFGKVAGDDDFKVRGIEAR
QRSTPPFIEDVQRDCLDRLDATRSPDAVLGRLERAIDELQAGNVAVERLVERNRVSKPLEGYSQNTQNVAALKRAREQDL
AVHPGQDIEYVVVDDEKSSRDRVALAHEAIETYDTSYYETQLVRAVESVLSPLGWDRSNIHRELSGERDAVLTSFGASNE
LS

Sequences:

>Translated_722_residues
MPFSIDFLDDGRVLEWEATADGAVATERDNYTPRFYVAARDPDADLDLTTLQSVYDQHPDVVATEMVARRPGFRRDEEAV
LAVDVAHIDRVTPLARQARQLSAYPVGDLACFNVDFSREFRYCLETGADPTPASELSTLRLSVPVTETSNDIYEELSVAG
DAVTGSSTDILAAVQGALEAHDPDVLVCSTSEIVPTLHEMATDAGVDDFSLSRWPDVDYQQLASRSTYSSYGRVGHSPAR
YNVPGRAIIDESNTFFYGETNLDGVLDLVSRSKNPVQELAWASIGNVLTAIQICEANDRGVLVPWNSWRHEFYKPMGTLH
DADRGGFIFAPEVGLHENVHELDFSSLYPNIICTRNVSPDVIRCDCHSDRDDVPGLGYSICDDRGYLVDVLQPIIDARDE
IKAAIRRETDRDDPDEDRLAELEGRSGALKWILVACFGYQGFSNAKFGRIECHEAINAFAREILLMAKQRLEAGGWRVVH
GIVDSIWVTPDPDVDEEDREDLQTLATEITERVEIRLEHEAHYDWVAFVPQRESDAGALTKYFGKVAGDDDFKVRGIEAR
QRSTPPFIEDVQRDCLDRLDATRSPDAVLGRLERAIDELQAGNVAVERLVERNRVSKPLEGYSQNTQNVAALKRAREQDL
AVHPGQDIEYVVVDDEKSSRDRVALAHEAIETYDTSYYETQLVRAVESVLSPLGWDRSNIHRELSGERDAVLTSFGASNE
LS
>Mature_721_residues
PFSIDFLDDGRVLEWEATADGAVATERDNYTPRFYVAARDPDADLDLTTLQSVYDQHPDVVATEMVARRPGFRRDEEAVL
AVDVAHIDRVTPLARQARQLSAYPVGDLACFNVDFSREFRYCLETGADPTPASELSTLRLSVPVTETSNDIYEELSVAGD
AVTGSSTDILAAVQGALEAHDPDVLVCSTSEIVPTLHEMATDAGVDDFSLSRWPDVDYQQLASRSTYSSYGRVGHSPARY
NVPGRAIIDESNTFFYGETNLDGVLDLVSRSKNPVQELAWASIGNVLTAIQICEANDRGVLVPWNSWRHEFYKPMGTLHD
ADRGGFIFAPEVGLHENVHELDFSSLYPNIICTRNVSPDVIRCDCHSDRDDVPGLGYSICDDRGYLVDVLQPIIDARDEI
KAAIRRETDRDDPDEDRLAELEGRSGALKWILVACFGYQGFSNAKFGRIECHEAINAFAREILLMAKQRLEAGGWRVVHG
IVDSIWVTPDPDVDEEDREDLQTLATEITERVEIRLEHEAHYDWVAFVPQRESDAGALTKYFGKVAGDDDFKVRGIEARQ
RSTPPFIEDVQRDCLDRLDATRSPDAVLGRLERAIDELQAGNVAVERLVERNRVSKPLEGYSQNTQNVAALKRAREQDLA
VHPGQDIEYVVVDDEKSSRDRVALAHEAIETYDTSYYETQLVRAVESVLSPLGWDRSNIHRELSGERDAVLTSFGASNEL
S

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=402, Percent_Identity=23.3830845771144, Blast_Score=82, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559488, Length=426, Percent_Identity=23.4741784037559, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32565317, Length=169, Percent_Identity=28.9940828402367, Blast_Score=71, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324227, Length=400, Percent_Identity=25.5, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320101, Length=410, Percent_Identity=23.4146341463415, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24664937, Length=425, Percent_Identity=24, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 80563; Mature: 80432

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSIDFLDDGRVLEWEATADGAVATERDNYTPRFYVAARDPDADLDLTTLQSVYDQHPD
CCEEEEECCCCCEEEEECCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCC
VVATEMVARRPGFRRDEEAVLAVDVAHIDRVTPLARQARQLSAYPVGDLACFNVDFSREF
HHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCHHHHH
RYCLETGADPTPASELSTLRLSVPVTETSNDIYEELSVAGDAVTGSSTDILAAVQGALEA
HHHHHCCCCCCCCHHHCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
HDPDVLVCSTSEIVPTLHEMATDAGVDDFSLSRWPDVDYQQLASRSTYSSYGRVGHSPAR
CCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCC
YNVPGRAIIDESNTFFYGETNLDGVLDLVSRSKNPVQELAWASIGNVLTAIQICEANDRG
CCCCCCEEEECCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCE
VLVPWNSWRHEFYKPMGTLHDADRGGFIFAPEVGLHENVHELDFSSLYPNIICTRNVSPD
EEECCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHCCHHHHCCCEEEECCCCCC
VIRCDCHSDRDDVPGLGYSICDDRGYLVDVLQPIIDARDEIKAAIRRETDRDDPDEDRLA
EEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHH
ELEGRSGALKWILVACFGYQGFSNAKFGRIECHEAINAFAREILLMAKQRLEAGGWRVVH
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
GIVDSIWVTPDPDVDEEDREDLQTLATEITERVEIRLEHEAHYDWVAFVPQRESDAGALT
HHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHH
KYFGKVAGDDDFKVRGIEARQRSTPPFIEDVQRDCLDRLDATRSPDAVLGRLERAIDELQ
HHHHHHCCCCCCEEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AGNVAVERLVERNRVSKPLEGYSQNTQNVAALKRAREQDLAVHPGQDIEYVVVDDEKSSR
CCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
DRVALAHEAIETYDTSYYETQLVRAVESVLSPLGWDRSNIHRELSGERDAVLTSFGASNE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCCCC
LS
CC
>Mature Secondary Structure 
PFSIDFLDDGRVLEWEATADGAVATERDNYTPRFYVAARDPDADLDLTTLQSVYDQHPD
CEEEEECCCCCEEEEECCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCC
VVATEMVARRPGFRRDEEAVLAVDVAHIDRVTPLARQARQLSAYPVGDLACFNVDFSREF
HHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCHHHHH
RYCLETGADPTPASELSTLRLSVPVTETSNDIYEELSVAGDAVTGSSTDILAAVQGALEA
HHHHHCCCCCCCCHHHCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
HDPDVLVCSTSEIVPTLHEMATDAGVDDFSLSRWPDVDYQQLASRSTYSSYGRVGHSPAR
CCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCC
YNVPGRAIIDESNTFFYGETNLDGVLDLVSRSKNPVQELAWASIGNVLTAIQICEANDRG
CCCCCCEEEECCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCE
VLVPWNSWRHEFYKPMGTLHDADRGGFIFAPEVGLHENVHELDFSSLYPNIICTRNVSPD
EEECCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHCCHHHHCCCEEEECCCCCC
VIRCDCHSDRDDVPGLGYSICDDRGYLVDVLQPIIDARDEIKAAIRRETDRDDPDEDRLA
EEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHH
ELEGRSGALKWILVACFGYQGFSNAKFGRIECHEAINAFAREILLMAKQRLEAGGWRVVH
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
GIVDSIWVTPDPDVDEEDREDLQTLATEITERVEIRLEHEAHYDWVAFVPQRESDAGALT
HHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHH
KYFGKVAGDDDFKVRGIEARQRSTPPFIEDVQRDCLDRLDATRSPDAVLGRLERAIDELQ
HHHHHHCCCCCCEEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AGNVAVERLVERNRVSKPLEGYSQNTQNVAALKRAREQDLAVHPGQDIEYVVVDDEKSSR
CCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
DRVALAHEAIETYDTSYYETQLVRAVESVLSPLGWDRSNIHRELSGERDAVLTSFGASNE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCCCC
LS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8441634; 1762925; 1579479 [H]