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The map label for this gene is arcB

Identifier: 16120223

GI number: 16120223

Start: 244821

End: 245708

Strand: Reverse

Name: arcB

Synonym: VNG6315G

Alternate gene names: 16120223

Gene position: 245708-244821 (Counterclockwise)

Preceding gene: 16120224

Following gene: 16120222

Centisome position: 67.24

GC content: 61.94

Gene sequence:

>888_bases
ATGGAGCATCTAGTAGACATCAACGACGTCGAGAGCGAAGAGATCGAACAGCTGCTTGACCTGGCGGCGAGCATGAAGGA
GAACCCGGGCGAGTTCTCCGGCGTGATGGACAACAAGTCTCTCGTGATGTTGTTCGCGAAGCCGTCGACGCGAACACGGC
TGTCCTTCGAGACGGGAATGACCCAACTCGGTGGGCACGGCATCTTCTTCGAGATGGGGTCCTCGCAGCTCAGCCGCGGG
GAGCCGATCTCCGACGTGAGCCAGGTGATGTCCCGGTACGAGGACGCCATCATGGCTCGGTTGTTCGAGCACGACGAGAT
GATGGAGCTTGCTGAGAACGCGGACGTGCCAGTCGTCAACGGGCTGACCGACTTCCTGCACCCGTGTCAGGCGCTCACCG
ACATGTTCACGATGCAGGAGAAAGACCGCCTCGATACGCTTGCGTTCGTCGGGGACGGAAACAACGTCGCCCACTCCCTG
ATGCAGGCGTCCGCGAAGATGGGCGTCGACTGTCGGATCGCGACACCCGAGGGAATGGAGCCCGACGAGGAGATCCAGGA
CCGTGTCAGCGACGCCAACGTGACCGTCACCAACGATCCCTACGAGGCGGTTGATGGCGCGACCGCAGTCTACGGCGACG
TGTTCGTCAGCATGGGCGAAGAGGAGCAGCGCGAGGAGAAGCTCGCCGAGTTCGACGGGTTCCAGATCGATCAGGATCTC
ATGGACGCCGCCCGCGACGACGCGATCTTCATGCATTGTCTCCCGGCCCACCGTGGCGAAGAGGTCACGGCGGAGGTCGC
CGACGGTCCGCAGTCGGTGATCTTTGACCAGGCCGAAAACCGCATGCACGTGCAGAAGGCCATCGTCCACACGCTCGTCA
ACCAATAA

Upstream 100 bases:

>100_bases
TCGATGGGGCGGCCGGCACGACAGTCGTGCCAGCCGACGAGTGACCGCGAGTTGGGAACAGTCGAAGGATTAAATAGTCG
ACCCGGAGATACACAACACG

Downstream 100 bases:

>100_bases
CTGCATCGACCTCACTGCTTTTTTGTTGCCGTATGCGCTGTGTGAATGGCCAGCGTGAGCGGGACTTGTGGAACAAGCCG
CGGTAGTAGTACGGAGGCGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MEHLVDINDVESEEIEQLLDLAASMKENPGEFSGVMDNKSLVMLFAKPSTRTRLSFETGMTQLGGHGIFFEMGSSQLSRG
EPISDVSQVMSRYEDAIMARLFEHDEMMELAENADVPVVNGLTDFLHPCQALTDMFTMQEKDRLDTLAFVGDGNNVAHSL
MQASAKMGVDCRIATPEGMEPDEEIQDRVSDANVTVTNDPYEAVDGATAVYGDVFVSMGEEEQREEKLAEFDGFQIDQDL
MDAARDDAIFMHCLPAHRGEEVTAEVADGPQSVIFDQAENRMHVQKAIVHTLVNQ

Sequences:

>Translated_295_residues
MEHLVDINDVESEEIEQLLDLAASMKENPGEFSGVMDNKSLVMLFAKPSTRTRLSFETGMTQLGGHGIFFEMGSSQLSRG
EPISDVSQVMSRYEDAIMARLFEHDEMMELAENADVPVVNGLTDFLHPCQALTDMFTMQEKDRLDTLAFVGDGNNVAHSL
MQASAKMGVDCRIATPEGMEPDEEIQDRVSDANVTVTNDPYEAVDGATAVYGDVFVSMGEEEQREEKLAEFDGFQIDQDL
MDAARDDAIFMHCLPAHRGEEVTAEVADGPQSVIFDQAENRMHVQKAIVHTLVNQ
>Mature_295_residues
MEHLVDINDVESEEIEQLLDLAASMKENPGEFSGVMDNKSLVMLFAKPSTRTRLSFETGMTQLGGHGIFFEMGSSQLSRG
EPISDVSQVMSRYEDAIMARLFEHDEMMELAENADVPVVNGLTDFLHPCQALTDMFTMQEKDRLDTLAFVGDGNNVAHSL
MQASAKMGVDCRIATPEGMEPDEEIQDRVSDANVTVTNDPYEAVDGATAVYGDVFVSMGEEEQREEKLAEFDGFQIDQDL
MDAARDDAIFMHCLPAHRGEEVTAEVADGPQSVIFDQAENRMHVQKAIVHTLVNQ

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=302, Percent_Identity=37.7483443708609, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=26.0450160771704, Blast_Score=98, Evalue=7e-21,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=34.472049689441, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786469, Length=322, Percent_Identity=34.472049689441, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=29.6774193548387, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI48994908, Length=345, Percent_Identity=23.768115942029, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=27.2445820433437, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=321, Percent_Identity=32.7102803738318, Blast_Score=182, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6322331, Length=302, Percent_Identity=24.5033112582781, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=26.797385620915, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTCC_HALSA (Q48296)

Other databases:

- EMBL:   X81712
- EMBL:   X80931
- EMBL:   AE004438
- PIR:   S49261
- RefSeq:   NP_395811.1
- ProteinModelPortal:   Q48296
- SMR:   Q48296
- GeneID:   1449194
- GenomeReviews:   AE004438_GR
- KEGG:   hal:VNG6315G
- NMPDR:   fig|64091.1.peg.2495
- HOGENOM:   HBG579429
- OMA:   GATIHIS
- ProtClustDB:   PRK00779
- BioCyc:   HSP64091:VNG6315G-MONOMER
- BioCyc:   MetaCyc:MONOMER-662
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 32677; Mature: 32677

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 100-100 BINDING 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLVDINDVESEEIEQLLDLAASMKENPGEFSGVMDNKSLVMLFAKPSTRTRLSFETGM
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCCEEEHHHCH
TQLGGHGIFFEMGSSQLSRGEPISDVSQVMSRYEDAIMARLFEHDEMMELAENADVPVVN
HHHCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEH
GLTDFLHPCQALTDMFTMQEKDRLDTLAFVGDGNNVAHSLMQASAKMGVDCRIATPEGME
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCC
PDEEIQDRVSDANVTVTNDPYEAVDGATAVYGDVFVSMGEEEQREEKLAEFDGFQIDQDL
CHHHHHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
MDAARDDAIFMHCLPAHRGEEVTAEVADGPQSVIFDQAENRMHVQKAIVHTLVNQ
HHHHCCCEEEEEEECCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEHLVDINDVESEEIEQLLDLAASMKENPGEFSGVMDNKSLVMLFAKPSTRTRLSFETGM
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCCEEEHHHCH
TQLGGHGIFFEMGSSQLSRGEPISDVSQVMSRYEDAIMARLFEHDEMMELAENADVPVVN
HHHCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEH
GLTDFLHPCQALTDMFTMQEKDRLDTLAFVGDGNNVAHSLMQASAKMGVDCRIATPEGME
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCC
PDEEIQDRVSDANVTVTNDPYEAVDGATAVYGDVFVSMGEEEQREEKLAEFDGFQIDQDL
CHHHHHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
MDAARDDAIFMHCLPAHRGEEVTAEVADGPQSVIFDQAENRMHVQKAIVHTLVNQ
HHHHCCCEEEEEEECCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7868583; 8759859; 11016950