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The map label for this gene is aph

Identifier: 16120213

GI number: 16120213

Start: 232702

End: 234123

Strand: Reverse

Name: aph

Synonym: VNG6301G

Alternate gene names: 16120213

Gene position: 234123-232702 (Counterclockwise)

Preceding gene: 16120215

Following gene: 16120212

Centisome position: 64.07

GC content: 68.28

Gene sequence:

>1422_bases
ATGCCAACACCGCACACCACAGAGTCACCCAGTGTCGATCGGCGCACGTTCCTGGCGGGCCTCAGCGGGGCCGTCGCCGG
GGGCGCAGTCGCGTCACAGTTCCAGACAACGGCGCGCGCACAGGCCACGGCGTCCCCGGCTGCGAACGCCATCGCGTACA
TCGTCGATGGGATGGGGCAGACACAGATCTCCGCCGCGCGCTACCTCAACGCCTACAAGACAGCGCCCGAGCGCTTCCCG
CTGAACGTGAGTCCGGCCGAGACGCCGACCGGGTTCGACGCGTTCTCGTCCCGCGGTTCGATGACCACGTTCCCCGATGA
CCCCTACGAGACGACCACCGACTCGGCGGCCGCCGCAACCGCGTTCGCCAGCGGCGTCAAAACCTACAACGGAGCGATCG
GTGGCGTCCAAACCTCAGGCGGCGGGTTTCAGCGCGTCGATACGGTCCTCGAACGGGCCAGCGCCCAGGGGTACGCGACC
GGCCTCATCACGACGACCGAGGCGACACACGCCACACCCGCAGCGTTCGCGGCACACGTCGAGGATCGCGGCAACCAGAC
CGAGATCGCGCGCCAGTACATCGAAGAGACCCAGCCGGACGTGATTCTCGGGGGGCAGCGCCGCGACTTCGAGGCGGACG
CGTCCAACGGCGGCACGCTGGTGGATGCGGCCAGGGACAACGGCTACACGATCGCCGAAACGGCGGCTGAACTGGACGCG
GTCGACGACCCACCCGTACTCGGGCTGTTCAGCCAGGAGAGCCACCTCGACTACTACCTGGACCGCAAAAACGACCCCGA
GAACACACAACCCAACCTCGATGCGATGGTCGACGCGGGGGTTGACCTGCTCTCGTCGGCCGGCGACCCCGACAAGGGGT
TTTTCCTGCTGGTGGAGTCCGGGCGCGTCGATCACGCCGGCCACGCGAACTACCCCGCCCAGGTCGCCGAACAGTACGAA
GCCACGCAGGTGGCGGGCCAGTTGGTGGAGTACGCCGAGACGACCGCCGAGCCGACGTTCCTCGTTTCGACCGGCGACCA
CGAGTGTGGCGGTCTCACGCTGGGTCGCGACTCGCCCTACGAGGTCGAATACGACGTGCTCGCCGCGCAGAAAGCGACCA
CCAGCCGGCTTCGGGATCTCCTCGCCGGGGTCCGGAGCGCTGACGAACTGGAGTCGATCGTCGCCGCTCACACGGGCATC
ACCGCACTGACCGACCGAGAGGTGGCAAAGCTCCGCGACGCTCCGGGTTCGATCAGCACGATTCTCGCGGAGCGAGCCGG
GATCGCGTTCACCACCGACGGACACACGGGCACCGACGTGCCGGTGTTCGCACACGGCCCGAACGCGGCGCGGTTCGACG
CCGCACGCGACAACACTGCGGTCGCCGACGCGCTCGCTGCGGCGCTCGGTGTGTCCCTGTAA

Upstream 100 bases:

>100_bases
CGAAGGGCGCTGGGGTGACGCCACCGCATATATAGGCGTCCGGACGACTATTATAAGTGGCTTTGAGCGCAGTGGTGGTA
CAGCCGTGTAGAGCGATCAG

Downstream 100 bases:

>100_bases
GCCGCGGCCGCATCCGGTGTTTTTTACTGGTGGGTGTGCGGACGGAGCAGTATGTCACGCCCGTCCTGTGAGCCCTTGAC
AGCGAACGACGAGTTGTTCG

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MPTPHTTESPSVDRRTFLAGLSGAVAGGAVASQFQTTARAQATASPAANAIAYIVDGMGQTQISAARYLNAYKTAPERFP
LNVSPAETPTGFDAFSSRGSMTTFPDDPYETTTDSAAAATAFASGVKTYNGAIGGVQTSGGGFQRVDTVLERASAQGYAT
GLITTTEATHATPAAFAAHVEDRGNQTEIARQYIEETQPDVILGGQRRDFEADASNGGTLVDAARDNGYTIAETAAELDA
VDDPPVLGLFSQESHLDYYLDRKNDPENTQPNLDAMVDAGVDLLSSAGDPDKGFFLLVESGRVDHAGHANYPAQVAEQYE
ATQVAGQLVEYAETTAEPTFLVSTGDHECGGLTLGRDSPYEVEYDVLAAQKATTSRLRDLLAGVRSADELESIVAAHTGI
TALTDREVAKLRDAPGSISTILAERAGIAFTTDGHTGTDVPVFAHGPNAARFDAARDNTAVADALAAALGVSL

Sequences:

>Translated_473_residues
MPTPHTTESPSVDRRTFLAGLSGAVAGGAVASQFQTTARAQATASPAANAIAYIVDGMGQTQISAARYLNAYKTAPERFP
LNVSPAETPTGFDAFSSRGSMTTFPDDPYETTTDSAAAATAFASGVKTYNGAIGGVQTSGGGFQRVDTVLERASAQGYAT
GLITTTEATHATPAAFAAHVEDRGNQTEIARQYIEETQPDVILGGQRRDFEADASNGGTLVDAARDNGYTIAETAAELDA
VDDPPVLGLFSQESHLDYYLDRKNDPENTQPNLDAMVDAGVDLLSSAGDPDKGFFLLVESGRVDHAGHANYPAQVAEQYE
ATQVAGQLVEYAETTAEPTFLVSTGDHECGGLTLGRDSPYEVEYDVLAAQKATTSRLRDLLAGVRSADELESIVAAHTGI
TALTDREVAKLRDAPGSISTILAERAGIAFTTDGHTGTDVPVFAHGPNAARFDAARDNTAVADALAAALGVSL
>Mature_472_residues
PTPHTTESPSVDRRTFLAGLSGAVAGGAVASQFQTTARAQATASPAANAIAYIVDGMGQTQISAARYLNAYKTAPERFPL
NVSPAETPTGFDAFSSRGSMTTFPDDPYETTTDSAAAATAFASGVKTYNGAIGGVQTSGGGFQRVDTVLERASAQGYATG
LITTTEATHATPAAFAAHVEDRGNQTEIARQYIEETQPDVILGGQRRDFEADASNGGTLVDAARDNGYTIAETAAELDAV
DDPPVLGLFSQESHLDYYLDRKNDPENTQPNLDAMVDAGVDLLSSAGDPDKGFFLLVESGRVDHAGHANYPAQVAEQYEA
TQVAGQLVEYAETTAEPTFLVSTGDHECGGLTLGRDSPYEVEYDVLAAQKATTSRLRDLLAGVRSADELESIVAAHTGIT
ALTDREVAKLRDAPGSISTILAERAGIAFTTDGHTGTDVPVFAHGPNAARFDAARDNTAVADALAAALGVSL

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=488, Percent_Identity=28.2786885245902, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI94721246, Length=487, Percent_Identity=28.3367556468172, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI157266296, Length=487, Percent_Identity=28.7474332648871, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI116734717, Length=356, Percent_Identity=30.6179775280899, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI294660770, Length=478, Percent_Identity=27.4058577405858, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI294660772, Length=441, Percent_Identity=27.891156462585, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI48994877, Length=359, Percent_Identity=31.7548746518106, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320689, Length=504, Percent_Identity=29.7619047619048, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18859923, Length=329, Percent_Identity=33.1306990881459, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24651554, Length=352, Percent_Identity=33.2386363636364, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355151, Length=473, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21355981, Length=357, Percent_Identity=30.2521008403361, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355149, Length=476, Percent_Identity=27.7310924369748, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585213, Length=370, Percent_Identity=28.6486486486486, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24651556, Length=307, Percent_Identity=33.2247557003257, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921912, Length=357, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI85725200, Length=495, Percent_Identity=26.4646464646465, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI85815833, Length=495, Percent_Identity=26.4646464646465, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24657835, Length=353, Percent_Identity=25.2124645892351, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 49305; Mature: 49174

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPHTTESPSVDRRTFLAGLSGAVAGGAVASQFQTTARAQATASPAANAIAYIVDGMGQ
CCCCCCCCCCCCHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
TQISAARYLNAYKTAPERFPLNVSPAETPTGFDAFSSRGSMTTFPDDPYETTTDSAAAAT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHH
AFASGVKTYNGAIGGVQTSGGGFQRVDTVLERASAQGYATGLITTTEATHATPAAFAAHV
HHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHH
EDRGNQTEIARQYIEETQPDVILGGQRRDFEADASNGGTLVDAARDNGYTIAETAAELDA
HCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHCC
VDDPPVLGLFSQESHLDYYLDRKNDPENTQPNLDAMVDAGVDLLSSAGDPDKGFFLLVES
CCCCCEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEC
GRVDHAGHANYPAQVAEQYEATQVAGQLVEYAETTAEPTFLVSTGDHECGGLTLGRDSPY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCC
EVEYDVLAAQKATTSRLRDLLAGVRSADELESIVAAHTGITALTDREVAKLRDAPGSIST
EEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCHHHHHHHHCCCCHHHH
ILAERAGIAFTTDGHTGTDVPVFAHGPNAARFDAARDNTAVADALAAALGVSL
HHHHHCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTPHTTESPSVDRRTFLAGLSGAVAGGAVASQFQTTARAQATASPAANAIAYIVDGMGQ
CCCCCCCCCCCHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
TQISAARYLNAYKTAPERFPLNVSPAETPTGFDAFSSRGSMTTFPDDPYETTTDSAAAAT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHH
AFASGVKTYNGAIGGVQTSGGGFQRVDTVLERASAQGYATGLITTTEATHATPAAFAAHV
HHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHH
EDRGNQTEIARQYIEETQPDVILGGQRRDFEADASNGGTLVDAARDNGYTIAETAAELDA
HCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHCC
VDDPPVLGLFSQESHLDYYLDRKNDPENTQPNLDAMVDAGVDLLSSAGDPDKGFFLLVES
CCCCCEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEC
GRVDHAGHANYPAQVAEQYEATQVAGQLVEYAETTAEPTFLVSTGDHECGGLTLGRDSPY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCC
EVEYDVLAAQKATTSRLRDLLAGVRSADELESIVAAHTGITALTDREVAKLRDAPGSIST
EEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCHHHHHHHHCCCCHHHH
ILAERAGIAFTTDGHTGTDVPVFAHGPNAARFDAARDNTAVADALAAALGVSL
HHHHHCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]