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The map label for this gene is gldA

Identifier: 16120191

GI number: 16120191

Start: 208894

End: 209991

Strand: Direct

Name: gldA

Synonym: VNG6270G

Alternate gene names: 16120191

Gene position: 208894-209991 (Clockwise)

Preceding gene: 16120190

Following gene: 16120195

Centisome position: 57.16

GC content: 64.12

Gene sequence:

>1098_bases
ATGACGAGCGTATTCAAATCGCCATCGACGTACGTACAGGGACGCAACGTGACGACCGACATCGGCACGCACGCGGAATC
ACTCGGTGACACAGCATTGCTAGTCGCCGACGAGATCGTGATGGACATGATCGAGAGCGACGTGCACGAAAGCCTCGCGG
AAGCCGGACTCGACGGCTCATCGGTCGTGTTCAACGGGGAGTCCTCGGAGGACGAGATCGAGCGGATCGCGTCCGTTGCT
GTCGACGAGGGTGCCGACATCGTTATCGGGGCCGGAGGCGGCAAGGCCCTGGACACCGCGAAAGCAGTCCGCGAGGAGGT
CGGCGGCGCAATGGTGTCGATGCCGACGATCGCATCGATGGACGCGCCGACGAGTTCGCTGTCAGTGATCTACAGCGAGC
ACGGCGAGTTCGAGGATTACTGGTACTACGAGCAGCACCCGGACTTGGTGATCGTTGACACCGAAGTGGTCGCTGCAGCG
CCGGCTCGGTTCCTGCGCTCGGGGATCGCAGACGGGCTTGCAACGTGGTTCGAGGCGGACGCAGTCGCACAGTCCGGCGG
CGACAACGAGGTCGGTGGGAAACCAACCCGCGCGGGACACAAGCTCGCCGAGCTGTGCTATGAGACGCTCCGTGAGCACG
GTGCCGGCGCGCTGGACGCCGTCGAGCACGATGCCGTGACCGAGAGCGTCGACGCCGTGATCGAGGCGAACACGCTGCTC
AGCGGGCTGGGGTTCGAAAGCGGCGGGCTGGCGGCTGCCCACTCCGTGCACAACGGCCTCACGCAGCTCGCGGAAACGCA
CGACGCGACCCACGGTGAAAAGGTGAACATCGGTACGATCACGCAGCTCGTGCTGGAGGGGCATTCCGACGCGAGGATCG
AGGAGTTCATCGAGTTCTCGGTCGAACTCGGGTTGCCTGTCACCCTCGGTGAGATCGGGATCACGAACCCTGAACAGGTA
GACCTGGACGTGGTCGCGGAGGCGGCCTGTGACGAAGCCGAGACGATCCACGACGAGCCGTTTGACGTTACACCAGCAAT
GGTGCGTGATGCGTTGCTGACCGCCGACGAGATGGGGCGGCGGGTTCGGGATCGATAG

Upstream 100 bases:

>100_bases
GCACAGTATAATGGATGTATGACGTGGTTTCTCCGGAGATAATGCGTGTTCTCGTAAAGTATATGCGGCTCCCCGGTGAA
TTTTTAGTACGGCCTAACTC

Downstream 100 bases:

>100_bases
CGGGGTCCCGTACCACCGTTCCTGCTGGGCGGCCCGCCGAGATTCATGCGGGAGCTGGACGTGTCGGCGGCGAAAAGGGA
GGGGGGACTCCCTCCCCCCG

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH; GlyDH [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTSVFKSPSTYVQGRNVTTDIGTHAESLGDTALLVADEIVMDMIESDVHESLAEAGLDGSSVVFNGESSEDEIERIASVA
VDEGADIVIGAGGGKALDTAKAVREEVGGAMVSMPTIASMDAPTSSLSVIYSEHGEFEDYWYYEQHPDLVIVDTEVVAAA
PARFLRSGIADGLATWFEADAVAQSGGDNEVGGKPTRAGHKLAELCYETLREHGAGALDAVEHDAVTESVDAVIEANTLL
SGLGFESGGLAAAHSVHNGLTQLAETHDATHGEKVNIGTITQLVLEGHSDARIEEFIEFSVELGLPVTLGEIGITNPEQV
DLDVVAEAACDEAETIHDEPFDVTPAMVRDALLTADEMGRRVRDR

Sequences:

>Translated_365_residues
MTSVFKSPSTYVQGRNVTTDIGTHAESLGDTALLVADEIVMDMIESDVHESLAEAGLDGSSVVFNGESSEDEIERIASVA
VDEGADIVIGAGGGKALDTAKAVREEVGGAMVSMPTIASMDAPTSSLSVIYSEHGEFEDYWYYEQHPDLVIVDTEVVAAA
PARFLRSGIADGLATWFEADAVAQSGGDNEVGGKPTRAGHKLAELCYETLREHGAGALDAVEHDAVTESVDAVIEANTLL
SGLGFESGGLAAAHSVHNGLTQLAETHDATHGEKVNIGTITQLVLEGHSDARIEEFIEFSVELGLPVTLGEIGITNPEQV
DLDVVAEAACDEAETIHDEPFDVTPAMVRDALLTADEMGRRVRDR
>Mature_364_residues
TSVFKSPSTYVQGRNVTTDIGTHAESLGDTALLVADEIVMDMIESDVHESLAEAGLDGSSVVFNGESSEDEIERIASVAV
DEGADIVIGAGGGKALDTAKAVREEVGGAMVSMPTIASMDAPTSSLSVIYSEHGEFEDYWYYEQHPDLVIVDTEVVAAAP
ARFLRSGIADGLATWFEADAVAQSGGDNEVGGKPTRAGHKLAELCYETLREHGAGALDAVEHDAVTESVDAVIEANTLLS
GLGFESGGLAAAHSVHNGLTQLAETHDATHGEKVNIGTITQLVLEGHSDARIEEFIEFSVELGLPVTLGEIGITNPEQVD
LDVVAEAACDEAETIHDEPFDVTPAMVRDALLTADEMGRRVRDR

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Is also able to use various diols as substrates, such as propan-1,2-diol, butan-

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=361, Percent_Identity=48.1994459833795, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1786815, Length=302, Percent_Identity=33.7748344370861, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38476; Mature: 38344

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU ; PS00180 GLNA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVFKSPSTYVQGRNVTTDIGTHAESLGDTALLVADEIVMDMIESDVHESLAEAGLDGS
CCCCCCCCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVVFNGESSEDEIERIASVAVDEGADIVIGAGGGKALDTAKAVREEVGGAMVSMPTIASM
EEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEECCCHHCC
DAPTSSLSVIYSEHGEFEDYWYYEQHPDLVIVDTEVVAAAPARFLRSGIADGLATWFEAD
CCCCHHHEEEECCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAQSGGDNEVGGKPTRAGHKLAELCYETLREHGAGALDAVEHDAVTESVDAVIEANTLL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SGLGFESGGLAAAHSVHNGLTQLAETHDATHGEKVNIGTITQLVLEGHSDARIEEFIEFS
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCCCHHHHHHHHHH
VELGLPVTLGEIGITNPEQVDLDVVAEAACDEAETIHDEPFDVTPAMVRDALLTADEMGR
HHCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RVRDR
HHCCC
>Mature Secondary Structure 
TSVFKSPSTYVQGRNVTTDIGTHAESLGDTALLVADEIVMDMIESDVHESLAEAGLDGS
CCCCCCCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVVFNGESSEDEIERIASVAVDEGADIVIGAGGGKALDTAKAVREEVGGAMVSMPTIASM
EEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEECCCHHCC
DAPTSSLSVIYSEHGEFEDYWYYEQHPDLVIVDTEVVAAAPARFLRSGIADGLATWFEAD
CCCCHHHEEEECCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAQSGGDNEVGGKPTRAGHKLAELCYETLREHGAGALDAVEHDAVTESVDAVIEANTLL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SGLGFESGGLAAAHSVHNGLTQLAETHDATHGEKVNIGTITQLVLEGHSDARIEEFIEFS
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCCCHHHHHHHHHH
VELGLPVTLGEIGITNPEQVDLDVVAEAACDEAETIHDEPFDVTPAMVRDALLTADEMGR
HHCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RVRDR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]