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The map label for this gene is virB4

Identifier: 16119786

GI number: 16119786

Start: 185305

End: 187674

Strand: Direct

Name: virB4

Synonym: Atu6170

Alternate gene names: 16119786

Gene position: 185305-187674 (Clockwise)

Preceding gene: 16119784

Following gene: 16119787

Centisome position: 86.5

GC content: 55.23

Gene sequence:

>2370_bases
ATGCTCGGAGCAAGTGGCACAACCGAAAGGTCTGGCGAGGTCTATCTACCCTACGTCGGGCACGTCAGCGACCATATTGT
CCTTCTAGAAGATGGATCGATCATGACGATGGCGCACGTAAGTGGCATGGCCTTCGAACTCGAAGATGCCGAAATGCGCA
ATGCACGTTGCCGTGCATTTAATACGCTCTTGCGCAATATCGCTGATGATCATGTGTCAATATATGCTCACCTCGTACGT
CATGACGATGTGCCGCCGTCACCCGCGCGACATTTCCGCAGCGCTTTTTCCGCCAGTCTGAGCGAAGCTTTTGAGGAGCG
CGTTCTCTCCGGCAAACTCCTTCGCAATGACCACTTCCTTACGCTGATCGTGTCTCCCCGGGCCGCGCTTGGCAAAGTGA
GGAGAAGGTTCACCAAACGCTACAGACAAAAAGAAAACGATCTCACAGCTCAAACCAGGAACCTGGAAGATCTCTGGCAT
CTTGTCGCTGGCGCTCTCGAAGCGTACGGCCTGCGTCGTCTTGGTATTCGTGAGAAGCAAGATGTGCTTTTTACGGAGGT
TGGAGAAGCTCTGCGGCTGATAATGACTGGTCGATTCACGCCGGTTCCCGTCGTTAGCGGTTCGCTCGGCGCCTCGATCT
ATACCGACCGAGTTATTTGCGGCAAGCGGGGACTCGAGATCCGAACACCAAAAGATAGTTATGTGGGATCTATTTACTCG
TTTCGCGAATACCCCGCAACGACGCGACCGGGTATGCTCAACGTGCTACTGTCTCTCGATTTTCCGCTTGTTCTGACGCA
GAGCTTCTCGTTTCTGACTCGCTCGCAAGCCCACTCGAAGCTCAGCCTCAAGTCCAGCCAAATGTTGAGTTCTGGCGACA
AAGCCGTCACCCAAATCAGCAAGTTATCCGAGGCGGAGGACGCACTAGCGAGCAACGAATTCGTATTGGGGGCGCATCAT
GTGAGTCTTTGCATATATGCAAATGATCTCAATAATCTTGCAGATAGAGGTGCCCGCGCCCGGACGCGATTGGCGGATGC
GGGAGCTGTTGTTGTCCAAGAGGGCATCGGCATGGAGGCGGCTTATTGGTCGCAGCTGCCAGGCAACTATAAGTGGCGCA
CGCGTCCGGGAGCGATCACATCGCGCAACTTCGCTGGTTTAGTCTCATTCGAGAATTTTCCCGAGGGATCCGGCTCAGGT
CACTGGGGCAACGCGATTGCGCGCTTTCGTACCAATGGTGGAACCCCTTTCGACTACATCCCGCACGAGCACGATGTCGG
CATGACGGCGATATTCGGTCCCATCGGGAGGGGTAAAACGACGCTCATGACCTTTATCCTCGCGATGCTCGAGCAGAGCA
TGGTCGACCGCGCGGGTGCGGTTGTCCTCTTCGACAAGGACCGCGGCAGTGAGCTGCTGGTTCGCGCCACCGGGGGAACA
TATTTGGCGCTCCGTAGAGGAGCGCCGAGCGGATTGGCGCCATTGCGTGGCCTGGAAAATACAGCGGCTTCACATGATTT
TCTGCGCGAATGGATCGTGGCGCTCATTGAGAGCGATGGCCGTGGAGGAATATCCCCCGAGGAAAATCGCCGTCTGGTGC
GGGGTATCCATCGGCAGCTCTCGTTTGATCCCCACATGCGCTCAATCGCGGGGTTACGCGAATTTTTGTTGCATGGACCC
GCCGAAGGGGCGGGAGCGAGACTCCAACGCTGGTGCCGTGGCAATGCACTAGGCTGGGCGTTCGACGGCGAGCTCGACGA
AGTAAAGTTGGATCCTTCGATTACTGGTTTCGACATGACGCATCTTCTCGAATATGAGGAAGTATGCGCTGCCGCCGCAG
CATATCTTCTGCACCGCATTGGAGCCATGGTTGACGGCCGTCGGTTTGTGATGAGTTGCGATGAGTTTCGCGCCTATTTG
CTAAATCCTAAATTTGCGGCGGTCGTCGACAAGTTCCTGCTTACTGTCCGCAAAAACAATGGGATGCTGATACTGGCAAC
GCAGCAACCTGAGCATGTCCTGGAATCGCAGCTAGGCGCCAGTCTCGTCGCGCAATGTATGACGAAGATTTTCTATCCTT
CACCCACGGCAGATCGATCGGCTTACATCGATGGACTGAAATGTACTGAAAAGGAATTTCAGGCGATCCGTGAAGACATG
GCAGTAGGTAGCCGGAAGTTTCTGCTTAAACGAGAAAGCGGAAGCGTCGTCTGCGAATTCGATCTGCGGGAAATGCGCGA
ATATGTCGCCGTACTTTCGGGGCGCGCCAACACGGTGCGCTTCGCGGATCAGCTTCGCAAAGTACAGGGGGACAACCCAT
CAGCCTGGCTCAGCGAATTTATGGCTCGTTACCACGAGGCAAAAGATTGA

Upstream 100 bases:

>100_bases
TGCAGACGGCGGGAAGGAGCGTTGATGGTCTGATTTGGGGCGGCGCAAGCGTTAGCCCAAATCCAATCAAGGTTCCCGCG
CGAGGGAGAGGAATGGCGTA

Downstream 100 bases:

>100_bases
TCAAAGGTGGGGAACTATGAAGATCATGCAACTTGTTGCTGCGGCCATGGCCGTCAGCCTTCTTTCGGTCGGGCCCGCGC
GGGCGCAGTTCGTTGTCAGC

Product: type IV secretion system protein VirB4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MLGASGTTERSGEVYLPYVGHVSDHIVLLEDGSIMTMAHVSGMAFELEDAEMRNARCRAFNTLLRNIADDHVSIYAHLVR
HDDVPPSPARHFRSAFSASLSEAFEERVLSGKLLRNDHFLTLIVSPRAALGKVRRRFTKRYRQKENDLTAQTRNLEDLWH
LVAGALEAYGLRRLGIREKQDVLFTEVGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGKRGLEIRTPKDSYVGSIYS
FREYPATTRPGMLNVLLSLDFPLVLTQSFSFLTRSQAHSKLSLKSSQMLSSGDKAVTQISKLSEAEDALASNEFVLGAHH
VSLCIYANDLNNLADRGARARTRLADAGAVVVQEGIGMEAAYWSQLPGNYKWRTRPGAITSRNFAGLVSFENFPEGSGSG
HWGNAIARFRTNGGTPFDYIPHEHDVGMTAIFGPIGRGKTTLMTFILAMLEQSMVDRAGAVVLFDKDRGSELLVRATGGT
YLALRRGAPSGLAPLRGLENTAASHDFLREWIVALIESDGRGGISPEENRRLVRGIHRQLSFDPHMRSIAGLREFLLHGP
AEGAGARLQRWCRGNALGWAFDGELDEVKLDPSITGFDMTHLLEYEEVCAAAAAYLLHRIGAMVDGRRFVMSCDEFRAYL
LNPKFAAVVDKFLLTVRKNNGMLILATQQPEHVLESQLGASLVAQCMTKIFYPSPTADRSAYIDGLKCTEKEFQAIREDM
AVGSRKFLLKRESGSVVCEFDLREMREYVAVLSGRANTVRFADQLRKVQGDNPSAWLSEFMARYHEAKD

Sequences:

>Translated_789_residues
MLGASGTTERSGEVYLPYVGHVSDHIVLLEDGSIMTMAHVSGMAFELEDAEMRNARCRAFNTLLRNIADDHVSIYAHLVR
HDDVPPSPARHFRSAFSASLSEAFEERVLSGKLLRNDHFLTLIVSPRAALGKVRRRFTKRYRQKENDLTAQTRNLEDLWH
LVAGALEAYGLRRLGIREKQDVLFTEVGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGKRGLEIRTPKDSYVGSIYS
FREYPATTRPGMLNVLLSLDFPLVLTQSFSFLTRSQAHSKLSLKSSQMLSSGDKAVTQISKLSEAEDALASNEFVLGAHH
VSLCIYANDLNNLADRGARARTRLADAGAVVVQEGIGMEAAYWSQLPGNYKWRTRPGAITSRNFAGLVSFENFPEGSGSG
HWGNAIARFRTNGGTPFDYIPHEHDVGMTAIFGPIGRGKTTLMTFILAMLEQSMVDRAGAVVLFDKDRGSELLVRATGGT
YLALRRGAPSGLAPLRGLENTAASHDFLREWIVALIESDGRGGISPEENRRLVRGIHRQLSFDPHMRSIAGLREFLLHGP
AEGAGARLQRWCRGNALGWAFDGELDEVKLDPSITGFDMTHLLEYEEVCAAAAAYLLHRIGAMVDGRRFVMSCDEFRAYL
LNPKFAAVVDKFLLTVRKNNGMLILATQQPEHVLESQLGASLVAQCMTKIFYPSPTADRSAYIDGLKCTEKEFQAIREDM
AVGSRKFLLKRESGSVVCEFDLREMREYVAVLSGRANTVRFADQLRKVQGDNPSAWLSEFMARYHEAKD
>Mature_789_residues
MLGASGTTERSGEVYLPYVGHVSDHIVLLEDGSIMTMAHVSGMAFELEDAEMRNARCRAFNTLLRNIADDHVSIYAHLVR
HDDVPPSPARHFRSAFSASLSEAFEERVLSGKLLRNDHFLTLIVSPRAALGKVRRRFTKRYRQKENDLTAQTRNLEDLWH
LVAGALEAYGLRRLGIREKQDVLFTEVGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGKRGLEIRTPKDSYVGSIYS
FREYPATTRPGMLNVLLSLDFPLVLTQSFSFLTRSQAHSKLSLKSSQMLSSGDKAVTQISKLSEAEDALASNEFVLGAHH
VSLCIYANDLNNLADRGARARTRLADAGAVVVQEGIGMEAAYWSQLPGNYKWRTRPGAITSRNFAGLVSFENFPEGSGSG
HWGNAIARFRTNGGTPFDYIPHEHDVGMTAIFGPIGRGKTTLMTFILAMLEQSMVDRAGAVVLFDKDRGSELLVRATGGT
YLALRRGAPSGLAPLRGLENTAASHDFLREWIVALIESDGRGGISPEENRRLVRGIHRQLSFDPHMRSIAGLREFLLHGP
AEGAGARLQRWCRGNALGWAFDGELDEVKLDPSITGFDMTHLLEYEEVCAAAAAYLLHRIGAMVDGRRFVMSCDEFRAYL
LNPKFAAVVDKFLLTVRKNNGMLILATQQPEHVLESQLGASLVAQCMTKIFYPSPTADRSAYIDGLKCTEKEFQAIREDM
AVGSRKFLLKRESGSVVCEFDLREMREYVAVLSGRANTVRFADQLRKVQGDNPSAWLSEFMARYHEAKD

Specific function: A possible function of virB4 might be to provide the energy, via hydrolysis of ATP, for translocation of virulence proteins of the transfer of a T-DNA-protein complex across the agrobacterium membrane

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIRB4_AGRT5 (P17794)

Other databases:

- EMBL:   X53264
- EMBL:   J03320
- EMBL:   AE007871
- PIR:   AH3248
- PIR:   S12344
- RefSeq:   NP_396491.1
- ProteinModelPortal:   P17794
- STRING:   P17794
- GeneID:   1137493
- GenomeReviews:   AE007871_GR
- KEGG:   atu:Atu6170
- eggNOG:   COG3451
- HOGENOM:   HBG395345
- OMA:   LSGRANT
- ProtClustDB:   PRK13853
- BioCyc:   ATUM176299-1:ATU6170-MONOMER
- InterPro:   IPR004346
- InterPro:   IPR018145
- TIGRFAMs:   TIGR00929

Pfam domain/function: PF03135 CagE_TrbE_VirB

EC number: NA

Molecular weight: Translated: 87478; Mature: 87478

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGASGTTERSGEVYLPYVGHVSDHIVLLEDGSIMTMAHVSGMAFELEDAEMRNARCRAF
CCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCEEEECCHHHHHHHHHHH
NTLLRNIADDHVSIYAHLVRHDDVPPSPARHFRSAFSASLSEAFEERVLSGKLLRNDHFL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEE
TLIVSPRAALGKVRRRFTKRYRQKENDLTAQTRNLEDLWHLVAGALEAYGLRRLGIREKQ
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCHHHCCCCCHH
DVLFTEVGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGKRGLEIRTPKDSYVGSIYS
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHCEEEECCCCCEEECCCHHHHHHHHH
FREYPATTRPGMLNVLLSLDFPLVLTQSFSFLTRSQAHSKLSLKSSQMLSSGDKAVTQIS
HHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KLSEAEDALASNEFVLGAHHVSLCIYANDLNNLADRGARARTRLADAGAVVVQEGIGMEA
HHHHHHHHHCCCCEEEEEEEEEEEEEECCHHHHHHCCHHHHHHHHCCCCEEEECCCCCCH
AYWSQLPGNYKWRTRPGAITSRNFAGLVSFENFPEGSGSGHWGNAIARFRTNGGTPFDYI
HHHHCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHEEECCCCCCCCCC
PHEHDVGMTAIFGPIGRGKTTLMTFILAMLEQSMVDRAGAVVLFDKDRGSELLVRATGGT
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCE
YLALRRGAPSGLAPLRGLENTAASHDFLREWIVALIESDGRGGISPEENRRLVRGIHRQL
EEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
SFDPHMRSIAGLREFLLHGPAEGAGARLQRWCRGNALGWAFDGELDEVKLDPSITGFDMT
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCCCHH
HLLEYEEVCAAAAAYLLHRIGAMVDGRRFVMSCDEFRAYLLNPKFAAVVDKFLLTVRKNN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHCCCHHHHHHHHHHHHEECCC
GMLILATQQPEHVLESQLGASLVAQCMTKIFYPSPTADRSAYIDGLKCTEKEFQAIREDM
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHH
AVGSRKFLLKRESGSVVCEFDLREMREYVAVLSGRANTVRFADQLRKVQGDNPSAWLSEF
HCCCHHEEEEECCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
MARYHEAKD
HHHHHCCCC
>Mature Secondary Structure
MLGASGTTERSGEVYLPYVGHVSDHIVLLEDGSIMTMAHVSGMAFELEDAEMRNARCRAF
CCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCEEEECCHHHHHHHHHHH
NTLLRNIADDHVSIYAHLVRHDDVPPSPARHFRSAFSASLSEAFEERVLSGKLLRNDHFL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEE
TLIVSPRAALGKVRRRFTKRYRQKENDLTAQTRNLEDLWHLVAGALEAYGLRRLGIREKQ
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCHHHCCCCCHH
DVLFTEVGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGKRGLEIRTPKDSYVGSIYS
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHCEEEECCCCCEEECCCHHHHHHHHH
FREYPATTRPGMLNVLLSLDFPLVLTQSFSFLTRSQAHSKLSLKSSQMLSSGDKAVTQIS
HHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KLSEAEDALASNEFVLGAHHVSLCIYANDLNNLADRGARARTRLADAGAVVVQEGIGMEA
HHHHHHHHHCCCCEEEEEEEEEEEEEECCHHHHHHCCHHHHHHHHCCCCEEEECCCCCCH
AYWSQLPGNYKWRTRPGAITSRNFAGLVSFENFPEGSGSGHWGNAIARFRTNGGTPFDYI
HHHHCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHEEECCCCCCCCCC
PHEHDVGMTAIFGPIGRGKTTLMTFILAMLEQSMVDRAGAVVLFDKDRGSELLVRATGGT
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCE
YLALRRGAPSGLAPLRGLENTAASHDFLREWIVALIESDGRGGISPEENRRLVRGIHRQL
EEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
SFDPHMRSIAGLREFLLHGPAEGAGARLQRWCRGNALGWAFDGELDEVKLDPSITGFDMT
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCCCHH
HLLEYEEVCAAAAAYLLHRIGAMVDGRRFVMSCDEFRAYLLNPKFAAVVDKFLLTVRKNN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHCCCHHHHHHHHHHHHEECCC
GMLILATQQPEHVLESQLGASLVAQCMTKIFYPSPTADRSAYIDGLKCTEKEFQAIREDM
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHH
AVGSRKFLLKRESGSVVCEFDLREMREYVAVLSGRANTVRFADQLRKVQGDNPSAWLSEF
HCCCHHEEEEECCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
MARYHEAKD
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2370849; 2194232; 2233252; 11743193; 11743194