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The map label for this gene is miaB

Identifier: 160903280

GI number: 160903280

Start: 2033661

End: 2034980

Strand: Direct

Name: miaB

Synonym: Pmob_1849

Alternate gene names: 160903280

Gene position: 2033661-2034980 (Clockwise)

Preceding gene: 160903279

Following gene: 160903281

Centisome position: 93.74

GC content: 33.03

Gene sequence:

>1320_bases
TTGAAATTTTACATACGGACATTTGGTTGTCAAATGAATATTAATGAAAGTGAAATAATGGCAGGTCTTTTGAAAGAAGA
AGGCTTCGAATGGACTGAAAATCCAAAAGAAGCCGACATAATATTGATAAATAGTTGTGCTGTTAGAGAAAAAGCAGAAA
ATAAAATGTATGGTGCCATAGGCGGTTACGGTAAACTAAAAGATGAAAATAAGAATTTAATCTTAGGTGTAGGTGGTTGC
TCTGCAGAAAAAGAAAGGGAGAATCTGCTTGAAAGATTTAAAAATATAGATTTTGTCTTTGGCACTAGAAATGTGGTAGA
CATAGGAAATTTGGTAAAGCGTGCTTTAAATGGAAAAAGATTTGCTGATTTTAGCGACAAATTGAATGATGTCAATTACG
ATATTCCAAAGATGCCAATAAGTAAACATCATGCTTGGATTACTATCATATACGGATGCAACAAATATTGCTCGTACTGT
ATAGTTCCATATACCAGAGGTTTTGAAAAAAGTAGACCTATGGAAGATATAATAAGAGAAGTTGAAAGCTATGCAAAAAA
GGGCTACAAAGAAATAACCTTTTTAGGTCAAAATGTTGATTCCTACGGTAAGGACTTTGGAGATAAAAAATCTAAATTAG
ACCTTCTCATACAAAAGGCAGCTGAATTTGATTCCATAAAACGAATTTGGTTTTTAACCTCATATCCATCGGATATAACC
GATTCTTTGATACAAACTGTTGCCAACGAAGAAAAAGCGGCAAATTATTTTCATCTTCCAGCTCAATCGGGAAGCAACAA
GATTCTTAAAGCGATGAATAGAAAATACACAAGAGAAGAGTTCATTGAACTTGTTAATAAGGTAAAGAAAGAGGTAGCTA
ACGTTACGGTATCCAGTGATTTTATAACGGGCTTTCCATCTGAAACAGATGAGGATTTTGAAGAAACGGTAGATTTGATT
AAGCAATGTAGATTTGAGAGAATAAATATCGCAGAATATTCCCCAAGAGAAGGGACCATAGCTTATAAATATCAAAATGA
CGATGTCCCTAAACATATCAAAAATAAAAGATTGCAGTATCTCATGGAGTTACAAAAAAGGATAAACCTTGAAGAAAATG
AAAAGTATCTTGAACAAGAAGTTGTTATAATTCAAGAGGGAAAAGCGGGTAAAAATGGTACTTATATGGGAAGAACTATG
AACAATAAATTAATAATCTTCGAAAGCAATGAAGAGTTGAACGGTGAATTTTTGAAAGTAAAGGTCAATAAAATTACACC
AGGTCCATTGTATGGTGAGGTTGTAAACAATCTTTACTGA

Upstream 100 bases:

>100_bases
TACCTTCAGAAATAGAAGACGAAGACGAAAAATTTTAATTTTTGGTTCTACATAATACACCGGGGGATAGCTCCCGGTTT
TTTTATAAGAGGTGATAATA

Downstream 100 bases:

>100_bases
CGGCCTAATAGATAGAAAAAAGGGGTGTTCACATTTGATACATGGAAAAGTATATGAATCTTTATCGTTTTATAGCCGAG
CACTGAAAAGTGATGTGAAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKFYIRTFGCQMNINESEIMAGLLKEEGFEWTENPKEADIILINSCAVREKAENKMYGAIGGYGKLKDENKNLILGVGGC
SAEKERENLLERFKNIDFVFGTRNVVDIGNLVKRALNGKRFADFSDKLNDVNYDIPKMPISKHHAWITIIYGCNKYCSYC
IVPYTRGFEKSRPMEDIIREVESYAKKGYKEITFLGQNVDSYGKDFGDKKSKLDLLIQKAAEFDSIKRIWFLTSYPSDIT
DSLIQTVANEEKAANYFHLPAQSGSNKILKAMNRKYTREEFIELVNKVKKEVANVTVSSDFITGFPSETDEDFEETVDLI
KQCRFERINIAEYSPREGTIAYKYQNDDVPKHIKNKRLQYLMELQKRINLEENEKYLEQEVVIIQEGKAGKNGTYMGRTM
NNKLIIFESNEELNGEFLKVKVNKITPGPLYGEVVNNLY

Sequences:

>Translated_439_residues
MKFYIRTFGCQMNINESEIMAGLLKEEGFEWTENPKEADIILINSCAVREKAENKMYGAIGGYGKLKDENKNLILGVGGC
SAEKERENLLERFKNIDFVFGTRNVVDIGNLVKRALNGKRFADFSDKLNDVNYDIPKMPISKHHAWITIIYGCNKYCSYC
IVPYTRGFEKSRPMEDIIREVESYAKKGYKEITFLGQNVDSYGKDFGDKKSKLDLLIQKAAEFDSIKRIWFLTSYPSDIT
DSLIQTVANEEKAANYFHLPAQSGSNKILKAMNRKYTREEFIELVNKVKKEVANVTVSSDFITGFPSETDEDFEETVDLI
KQCRFERINIAEYSPREGTIAYKYQNDDVPKHIKNKRLQYLMELQKRINLEENEKYLEQEVVIIQEGKAGKNGTYMGRTM
NNKLIIFESNEELNGEFLKVKVNKITPGPLYGEVVNNLY
>Mature_439_residues
MKFYIRTFGCQMNINESEIMAGLLKEEGFEWTENPKEADIILINSCAVREKAENKMYGAIGGYGKLKDENKNLILGVGGC
SAEKERENLLERFKNIDFVFGTRNVVDIGNLVKRALNGKRFADFSDKLNDVNYDIPKMPISKHHAWITIIYGCNKYCSYC
IVPYTRGFEKSRPMEDIIREVESYAKKGYKEITFLGQNVDSYGKDFGDKKSKLDLLIQKAAEFDSIKRIWFLTSYPSDIT
DSLIQTVANEEKAANYFHLPAQSGSNKILKAMNRKYTREEFIELVNKVKKEVANVTVSSDFITGFPSETDEDFEETVDLI
KQCRFERINIAEYSPREGTIAYKYQNDDVPKHIKNKRLQYLMELQKRINLEENEKYLEQEVVIIQEGKAGKNGTYMGRTM
NNKLIIFESNEELNGEFLKVKVNKITPGPLYGEVVNNLY

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=32.7044025157233, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=32.7044025157233, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI93277076, Length=431, Percent_Identity=27.6102088167053, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=36.7117117117117, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=27.2522522522523, Blast_Score=168, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=459, Percent_Identity=30.0653594771242, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71996771, Length=354, Percent_Identity=26.271186440678, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356207, Length=440, Percent_Identity=32.7272727272727, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19922432, Length=449, Percent_Identity=25.8351893095768, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PETMO (A9BGV7)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568861.1
- ProteinModelPortal:   A9BGV7
- SMR:   A9BGV7
- GeneID:   5756968
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1849
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14330
- BioCyc:   PMOB403833:PMOB_1849-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50597; Mature: 50597

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYIRTFGCQMNINESEIMAGLLKEEGFEWTENPKEADIILINSCAVREKAENKMYGAI
CEEEEEEECEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCCEECC
GGYGKLKDENKNLILGVGGCSAEKERENLLERFKNIDFVFGTRNVVDIGNLVKRALNGKR
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCC
FADFSDKLNDVNYDIPKMPISKHHAWITIIYGCNKYCSYCIVPYTRGFEKSRPMEDIIRE
CCCHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHCEEEEECCCCCCCCCCHHHHHHH
VESYAKKGYKEITFLGQNVDSYGKDFGDKKSKLDLLIQKAAEFDSIKRIWFLTSYPSDIT
HHHHHHCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHH
DSLIQTVANEEKAANYFHLPAQSGSNKILKAMNRKYTREEFIELVNKVKKEVANVTVSSD
HHHHHHHHCHHHCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEECC
FITGFPSETDEDFEETVDLIKQCRFERINIAEYSPREGTIAYKYQNDDVPKHIKNKRLQY
CEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LMELQKRINLEENEKYLEQEVVIIQEGKAGKNGTYMGRTMNNKLIIFESNEELNGEFLKV
HHHHHHHCCCCHHHHHHHHEEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEEE
KVNKITPGPLYGEVVNNLY
EEECCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKFYIRTFGCQMNINESEIMAGLLKEEGFEWTENPKEADIILINSCAVREKAENKMYGAI
CEEEEEEECEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCCEECC
GGYGKLKDENKNLILGVGGCSAEKERENLLERFKNIDFVFGTRNVVDIGNLVKRALNGKR
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCC
FADFSDKLNDVNYDIPKMPISKHHAWITIIYGCNKYCSYCIVPYTRGFEKSRPMEDIIRE
CCCHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHCEEEEECCCCCCCCCCHHHHHHH
VESYAKKGYKEITFLGQNVDSYGKDFGDKKSKLDLLIQKAAEFDSIKRIWFLTSYPSDIT
HHHHHHCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHH
DSLIQTVANEEKAANYFHLPAQSGSNKILKAMNRKYTREEFIELVNKVKKEVANVTVSSD
HHHHHHHHCHHHCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEECC
FITGFPSETDEDFEETVDLIKQCRFERINIAEYSPREGTIAYKYQNDDVPKHIKNKRLQY
CEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LMELQKRINLEENEKYLEQEVVIIQEGKAGKNGTYMGRTMNNKLIIFESNEELNGEFLKV
HHHHHHHCCCCHHHHHHHHEEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEEE
KVNKITPGPLYGEVVNNLY
EEECCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA