Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 160903223

GI number: 160903223

Start: 1962253

End: 1964577

Strand: Direct

Name: metH [H]

Synonym: Pmob_1792

Alternate gene names: 160903223

Gene position: 1962253-1964577 (Clockwise)

Preceding gene: 160903222

Following gene: 160903224

Centisome position: 90.45

GC content: 34.32

Gene sequence:

>2325_bases
ATGAATAGAAAAGAGTTTGCAGAAATATTGAATGAAAAGATACTCATTTTAGACGGAGGATATGGAACAGAATTTATAAA
AAGAGGATATAAAAATATTCCTGCTGAGATTTTAAACATCGAATATCCAGAAGTAGTTTACAATCTACAAAGTGAATACG
TAAAATCTGGAGCAGACATTCTTTTAACTAATACTTTTAGTGGAAATCGACGAAAACTCAAAGAGCTAAATTATGAAGAA
GCATTCGAAAAGGTCAACGTCAAAGCAGTTGAAATTGCCAAGCAAGCATCAAAAGGCAAAGCTTTGGTCTTTGGCGATCT
ATCTTCTCTAGGAGAGTATCCAAAGCCTATGGGAGCTCTTGAAATGGACGAAGCTATTGAGGAGTATTATCAACAGGCAA
AAGTGTTGTTTGAAAGCGGTGTAGATGGATTTATCGTTGAAACAATGACAGATATAAAAGAGCTAAAAGCTGCTGTATAT
GGAATAAGAAAAGTTACAGAAGATTTACCTTTAATAGCTCATATGACGTTTGAAGAGAACGGCCGTTCCGTTACGGGGAC
TTCTGTGGAAATCTTTGCGAATGTTTTTAATGATTTAGATGTAGATGTACTAGGAATTAACTGTACTTTGGGTCCCGAGG
ATCTTTTAAAGGTTTTTGAGCGTCTATCACTCTCAACAAACAAGTTTTTATCAGTCGAACCAAACGCTGGTAAACCCATT
TTTGATGGAAAAAATTTGGAATACAAAATGACGCCCGAAATATTTGGAATGTTTGTTGAAGACTATTTAGATTTAGGAGT
AAATATTATTGGAGGCTGTTGTGGTACCTCTCCAGAACATATAAAGGTAATTAGGAATATGGTAAAAAGAAGACCTAAAA
AAATAGTTAAAGAAATACCTATTATGTACTCTTCAAGAACGATCTTAAACAAATTTCATCCCTTTACCGTTATCGGAGAG
AGAATAAATCCTGCGGGGAACAAAAAATTTCAAAATGAAATTGAAGAATTCAACTTCGATAATGTAATAAAAAGGGCAAA
CAATCAAAAAAATGCAAAAGCCCATGCAATAGACGTTAACTTGGGTATCGAAAAGATTTTGAACGAAGAGCATTTCAAAA
AAATTATCATAGAATTAGACAAACACTCTTCTCTTCCTATTTCTTTTGACATTCAAAATATTGGGTTTTTAGAGAGCGCT
TTAAAAGAGTATCCAGGAAGACCAATAATCAATTCTTCTAAATTGAGTAAAAAAGATCTAGACAGAAAACTAGAGTTGAT
TAAAAAATATGGTGGGTTATTAATAGTATTAGCGCTTGAGAAAGAAATCTTAGAATCTGCAGAAGAAAGACTACAACTAA
TGCTAAGAAAATGGCCATACATAGAAAGTAAAGGAATAAGTAAAGATAGAATTATAGTTGACCCATTGGTGCTATCCCTT
GCTGCCAATAACGATCCAAATATTACTTTGGAGACAGTAAAATTACTCTCGCAAAATGGGTTCAATACCACTATGGGTCT
TTCGAATCTTAGCTTCGGTTTACCAAACAGAAATTATATAAATGCAGCTTTTCTCTCAAGGGCAAAAAGTAAAGGCTTGA
CTTCAGCCATCTTAAATCCAGAAGATGAGTTTCTTATGAACACGTTGAATGGCAACCTATTGTTGGATAAAAATATTGTA
ATACCAACTAAGGTTGAAAAACAAGATGAATTAACCGAGAAAATCCTACAAGGTGAAGAAGGTGAAGTAAAACGAATCAT
TGAAAATCAGTTAAAAGAGAAAAGTCCATTAGAAGTTAGTCAAGATGTGCTGGGTAAAGCGATGGAAAAGATCGGAGACC
TCTACGCACAAGGGAACATATATCTTCCTGAGTTATTGTTAGCTGCCGAAACTGTCAAACCCATTTTTGATTACCTAAAC
AATTTGATTCCTGAATCTCAAAACGATAAAAAAGCTAAAGTCGTACTAGCCACGGTAGAAGGAGATATACACGATATTGG
TAAAAATATAGTAGCAGCAGTATTAAGAAGCGCTAACTTCAAAATTATAGATCTTGGCAAAGATGTGGAAACTTCTATAA
TAATCGACGCTGTACAAAAAGAAAAACCAAATATATTAGGGCTCTCGGCTATGATGACAACTACCGTGGGAAAAATAGAA
GATGTGGTAAATGAACTAAAAAAATTGAGTCTTAATGTGAAAGTTATTGCTGGTGGTGCTTCTATGAACAGAAAATTAGC
TGAAACTTTTGGATGTGATGCCTACGCTAAAGATGCAAGCGAAGGGCTCAAGATATGCAAAAGTTGGGTCAATTTGAATT
CGTAA

Upstream 100 bases:

>100_bases
ATCGTCTGTAATTTACTCAAAACAGAGATAGTAGTTGTCAACAAAGAAACTGGTATCATAACTCCAAGAAAAAGTACTGT
ATGTATGATAGGGTGGTATG

Downstream 100 bases:

>100_bases
TTGTGTATCTTCCGATTTTTATATATCAAAAATGTTAATAAAAAGAAAAAGCTTTATGTCGATAAAACATGATAAAATTT
ATCAATTTTTCTAAAATACT

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MNRKEFAEILNEKILILDGGYGTEFIKRGYKNIPAEILNIEYPEVVYNLQSEYVKSGADILLTNTFSGNRRKLKELNYEE
AFEKVNVKAVEIAKQASKGKALVFGDLSSLGEYPKPMGALEMDEAIEEYYQQAKVLFESGVDGFIVETMTDIKELKAAVY
GIRKVTEDLPLIAHMTFEENGRSVTGTSVEIFANVFNDLDVDVLGINCTLGPEDLLKVFERLSLSTNKFLSVEPNAGKPI
FDGKNLEYKMTPEIFGMFVEDYLDLGVNIIGGCCGTSPEHIKVIRNMVKRRPKKIVKEIPIMYSSRTILNKFHPFTVIGE
RINPAGNKKFQNEIEEFNFDNVIKRANNQKNAKAHAIDVNLGIEKILNEEHFKKIIIELDKHSSLPISFDIQNIGFLESA
LKEYPGRPIINSSKLSKKDLDRKLELIKKYGGLLIVLALEKEILESAEERLQLMLRKWPYIESKGISKDRIIVDPLVLSL
AANNDPNITLETVKLLSQNGFNTTMGLSNLSFGLPNRNYINAAFLSRAKSKGLTSAILNPEDEFLMNTLNGNLLLDKNIV
IPTKVEKQDELTEKILQGEEGEVKRIIENQLKEKSPLEVSQDVLGKAMEKIGDLYAQGNIYLPELLLAAETVKPIFDYLN
NLIPESQNDKKAKVVLATVEGDIHDIGKNIVAAVLRSANFKIIDLGKDVETSIIIDAVQKEKPNILGLSAMMTTTVGKIE
DVVNELKKLSLNVKVIAGGASMNRKLAETFGCDAYAKDASEGLKICKSWVNLNS

Sequences:

>Translated_774_residues
MNRKEFAEILNEKILILDGGYGTEFIKRGYKNIPAEILNIEYPEVVYNLQSEYVKSGADILLTNTFSGNRRKLKELNYEE
AFEKVNVKAVEIAKQASKGKALVFGDLSSLGEYPKPMGALEMDEAIEEYYQQAKVLFESGVDGFIVETMTDIKELKAAVY
GIRKVTEDLPLIAHMTFEENGRSVTGTSVEIFANVFNDLDVDVLGINCTLGPEDLLKVFERLSLSTNKFLSVEPNAGKPI
FDGKNLEYKMTPEIFGMFVEDYLDLGVNIIGGCCGTSPEHIKVIRNMVKRRPKKIVKEIPIMYSSRTILNKFHPFTVIGE
RINPAGNKKFQNEIEEFNFDNVIKRANNQKNAKAHAIDVNLGIEKILNEEHFKKIIIELDKHSSLPISFDIQNIGFLESA
LKEYPGRPIINSSKLSKKDLDRKLELIKKYGGLLIVLALEKEILESAEERLQLMLRKWPYIESKGISKDRIIVDPLVLSL
AANNDPNITLETVKLLSQNGFNTTMGLSNLSFGLPNRNYINAAFLSRAKSKGLTSAILNPEDEFLMNTLNGNLLLDKNIV
IPTKVEKQDELTEKILQGEEGEVKRIIENQLKEKSPLEVSQDVLGKAMEKIGDLYAQGNIYLPELLLAAETVKPIFDYLN
NLIPESQNDKKAKVVLATVEGDIHDIGKNIVAAVLRSANFKIIDLGKDVETSIIIDAVQKEKPNILGLSAMMTTTVGKIE
DVVNELKKLSLNVKVIAGGASMNRKLAETFGCDAYAKDASEGLKICKSWVNLNS
>Mature_774_residues
MNRKEFAEILNEKILILDGGYGTEFIKRGYKNIPAEILNIEYPEVVYNLQSEYVKSGADILLTNTFSGNRRKLKELNYEE
AFEKVNVKAVEIAKQASKGKALVFGDLSSLGEYPKPMGALEMDEAIEEYYQQAKVLFESGVDGFIVETMTDIKELKAAVY
GIRKVTEDLPLIAHMTFEENGRSVTGTSVEIFANVFNDLDVDVLGINCTLGPEDLLKVFERLSLSTNKFLSVEPNAGKPI
FDGKNLEYKMTPEIFGMFVEDYLDLGVNIIGGCCGTSPEHIKVIRNMVKRRPKKIVKEIPIMYSSRTILNKFHPFTVIGE
RINPAGNKKFQNEIEEFNFDNVIKRANNQKNAKAHAIDVNLGIEKILNEEHFKKIIIELDKHSSLPISFDIQNIGFLESA
LKEYPGRPIINSSKLSKKDLDRKLELIKKYGGLLIVLALEKEILESAEERLQLMLRKWPYIESKGISKDRIIVDPLVLSL
AANNDPNITLETVKLLSQNGFNTTMGLSNLSFGLPNRNYINAAFLSRAKSKGLTSAILNPEDEFLMNTLNGNLLLDKNIV
IPTKVEKQDELTEKILQGEEGEVKRIIENQLKEKSPLEVSQDVLGKAMEKIGDLYAQGNIYLPELLLAAETVKPIFDYLN
NLIPESQNDKKAKVVLATVEGDIHDIGKNIVAAVLRSANFKIIDLGKDVETSIIIDAVQKEKPNILGLSAMMTTTVGKIE
DVVNELKKLSLNVKVIAGGASMNRKLAETFGCDAYAKDASEGLKICKSWVNLNS

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=890, Percent_Identity=27.1910112359551, Blast_Score=292, Evalue=7e-79,
Organism=Homo sapiens, GI13162290, Length=297, Percent_Identity=28.2828282828283, Blast_Score=90, Evalue=9e-18,
Organism=Escherichia coli, GI1790450, Length=871, Percent_Identity=26.0619977037887, Blast_Score=266, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17535405, Length=899, Percent_Identity=25.8064516129032, Blast_Score=274, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324982, Length=321, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 86433; Mature: 86433

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKEFAEILNEKILILDGGYGTEFIKRGYKNIPAEILNIEYPEVVYNLQSEYVKSGADI
CCHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCE
LLTNTFSGNRRKLKELNYEEAFEKVNVKAVEIAKQASKGKALVFGDLSSLGEYPKPMGAL
EEEECCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCC
EMDEAIEEYYQQAKVLFESGVDGFIVETMTDIKELKAAVYGIRKVTEDLPLIAHMTFEEN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GRSVTGTSVEIFANVFNDLDVDVLGINCTLGPEDLLKVFERLSLSTNKFLSVEPNAGKPI
CCEECCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCC
FDGKNLEYKMTPEIFGMFVEDYLDLGVNIIGGCCGTSPEHIKVIRNMVKRRPKKIVKEIP
CCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
IMYSSRTILNKFHPFTVIGERINPAGNKKFQNEIEEFNFDNVIKRANNQKNAKAHAIDVN
CCCCCHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEC
LGIEKILNEEHFKKIIIELDKHSSLPISFDIQNIGFLESALKEYPGRPIINSSKLSKKDL
CCHHHHHCHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
DRKLELIKKYGGLLIVLALEKEILESAEERLQLMLRKWPYIESKGISKDRIIVDPLVLSL
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHE
AANNDPNITLETVKLLSQNGFNTTMGLSNLSFGLPNRNYINAAFLSRAKSKGLTSAILNP
ECCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCC
EDEFLMNTLNGNLLLDKNIVIPTKVEKQDELTEKILQGEEGEVKRIIENQLKEKSPLEVS
CHHHHHHHCCCCEEEECCEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHH
QDVLGKAMEKIGDLYAQGNIYLPELLLAAETVKPIFDYLNNLIPESQNDKKAKVVLATVE
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEC
GDIHDIGKNIVAAVLRSANFKIIDLGKDVETSIIIDAVQKEKPNILGLSAMMTTTVGKIE
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
DVVNELKKLSLNVKVIAGGASMNRKLAETFGCDAYAKDASEGLKICKSWVNLNS
HHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRKEFAEILNEKILILDGGYGTEFIKRGYKNIPAEILNIEYPEVVYNLQSEYVKSGADI
CCHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCE
LLTNTFSGNRRKLKELNYEEAFEKVNVKAVEIAKQASKGKALVFGDLSSLGEYPKPMGAL
EEEECCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCC
EMDEAIEEYYQQAKVLFESGVDGFIVETMTDIKELKAAVYGIRKVTEDLPLIAHMTFEEN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GRSVTGTSVEIFANVFNDLDVDVLGINCTLGPEDLLKVFERLSLSTNKFLSVEPNAGKPI
CCEECCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCC
FDGKNLEYKMTPEIFGMFVEDYLDLGVNIIGGCCGTSPEHIKVIRNMVKRRPKKIVKEIP
CCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
IMYSSRTILNKFHPFTVIGERINPAGNKKFQNEIEEFNFDNVIKRANNQKNAKAHAIDVN
CCCCCHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEC
LGIEKILNEEHFKKIIIELDKHSSLPISFDIQNIGFLESALKEYPGRPIINSSKLSKKDL
CCHHHHHCHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
DRKLELIKKYGGLLIVLALEKEILESAEERLQLMLRKWPYIESKGISKDRIIVDPLVLSL
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHE
AANNDPNITLETVKLLSQNGFNTTMGLSNLSFGLPNRNYINAAFLSRAKSKGLTSAILNP
ECCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCC
EDEFLMNTLNGNLLLDKNIVIPTKVEKQDELTEKILQGEEGEVKRIIENQLKEKSPLEVS
CHHHHHHHCCCCEEEECCEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHH
QDVLGKAMEKIGDLYAQGNIYLPELLLAAETVKPIFDYLNNLIPESQNDKKAKVVLATVE
HHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEC
GDIHDIGKNIVAAVLRSANFKIIDLGKDVETSIIIDAVQKEKPNILGLSAMMTTTVGKIE
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
DVVNELKKLSLNVKVIAGGASMNRKLAETFGCDAYAKDASEGLKICKSWVNLNS
HHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]