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The map label for this gene is purR [H]

Identifier: 160903035

GI number: 160903035

Start: 1742607

End: 1743647

Strand: Direct

Name: purR [H]

Synonym: Pmob_1599

Alternate gene names: 160903035

Gene position: 1742607-1743647 (Clockwise)

Preceding gene: 160903034

Following gene: 160903036

Centisome position: 80.32

GC content: 29.78

Gene sequence:

>1041_bases
ATGCAAAAAACAACTATAAAAGACATCGCAAAGGCTTTGAATATCTCTCCCTCTACAGTTTCAAGAGCTTTAAATGGGAA
AAATGGCGTAAGCGAAAAACTCAGTGTAACAATTAGAGAAAAAGCTGAAGAACTTGGTTATGTTGGCAATTTTGCCGCCC
GTTCTTTAAAAACATCGCAATCTAAAACTATTGGAGTTACTGTTTCAGATATAAGAAATCCTTTCTTTTTAGATTTTCTA
AAAGGGGTAGACAATATTTTATTTCAAAGAGGTTATAAATTCCTTTTAACAGATTCAGGAGAAAATGTTGAAAAAGAAAA
AATTTATTTAATGTGGCTTATCGAACACGGAGTAGATGGTATTTTAGCGTCGCCTGTGACTGAAAGAGATGGAAAAAATA
ATTTAGAACTTTACAAAAAAATTAAGAATATAAGTATTCCAATTGTGTTTTATGATAGAGTCTTCCCTGAGGAATCCCAA
TTTGATTCAGTCACGGTGGATAATAAAGATAGTATTATTCAAGCTTTAAAATATTTATATAATAAAGGCCATAGAAAAAT
TGGTATTTTTCTTTCAAAGAGTGGTATTTATACTATAGAACAAAGATTAGAAGGCTTTATAGAGGGGTGCGAACTTTTGG
GAATTAAAATAAATGAAGAATGGATAGCAAAAGATTTGTTTCCCGAAGAAAAAACTTACGATAAATTGAAAAAATTGAAG
AAAAATCATAATCTTCCAACTGCAATAATTGCCACAAACAATAAAATAACCAAACAGTTAATTAGTGCTTCAAAAAAATT
AAACATATTAATACCGAATACTCTTTCAATTATGGGATACGATGATTTGACGGAAAATGAAATAATTTCACCCCCCATAA
CGTGCATCAAACAACCAGTCTTTGAAATAGGTAGAATAGCATCGACTATATTGTTAGGAAAAATAGAAAATGATGAAACT
AAAACAACTAAGATCGTGTTGAAAACAGAGATTGTTGAAAGAAATTCGATCATTAATTTGAATAAAAATACACTTAATTG
A

Upstream 100 bases:

>100_bases
TACTCATCGAGAGTTAATTTTGATTTTATTGTTTATTAATTTATAATAAGAATTAGTAACGCAAACGATTGCTTACTAAA
TGCTTATGGAGGGTAACCCC

Downstream 100 bases:

>100_bases
ATTTATTCTGTTTAAATTTTCAACTTTCATATATTACAACTAGGAGGTGTTTGGTATGAAAAAGGGTCTAACTGTTTTGT
TGTTAGTTACTGTTTTATTG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MQKTTIKDIAKALNISPSTVSRALNGKNGVSEKLSVTIREKAEELGYVGNFAARSLKTSQSKTIGVTVSDIRNPFFLDFL
KGVDNILFQRGYKFLLTDSGENVEKEKIYLMWLIEHGVDGILASPVTERDGKNNLELYKKIKNISIPIVFYDRVFPEESQ
FDSVTVDNKDSIIQALKYLYNKGHRKIGIFLSKSGIYTIEQRLEGFIEGCELLGIKINEEWIAKDLFPEEKTYDKLKKLK
KNHNLPTAIIATNNKITKQLISASKKLNILIPNTLSIMGYDDLTENEIISPPITCIKQPVFEIGRIASTILLGKIENDET
KTTKIVLKTEIVERNSIINLNKNTLN

Sequences:

>Translated_346_residues
MQKTTIKDIAKALNISPSTVSRALNGKNGVSEKLSVTIREKAEELGYVGNFAARSLKTSQSKTIGVTVSDIRNPFFLDFL
KGVDNILFQRGYKFLLTDSGENVEKEKIYLMWLIEHGVDGILASPVTERDGKNNLELYKKIKNISIPIVFYDRVFPEESQ
FDSVTVDNKDSIIQALKYLYNKGHRKIGIFLSKSGIYTIEQRLEGFIEGCELLGIKINEEWIAKDLFPEEKTYDKLKKLK
KNHNLPTAIIATNNKITKQLISASKKLNILIPNTLSIMGYDDLTENEIISPPITCIKQPVFEIGRIASTILLGKIENDET
KTTKIVLKTEIVERNSIINLNKNTLN
>Mature_346_residues
MQKTTIKDIAKALNISPSTVSRALNGKNGVSEKLSVTIREKAEELGYVGNFAARSLKTSQSKTIGVTVSDIRNPFFLDFL
KGVDNILFQRGYKFLLTDSGENVEKEKIYLMWLIEHGVDGILASPVTERDGKNNLELYKKIKNISIPIVFYDRVFPEESQ
FDSVTVDNKDSIIQALKYLYNKGHRKIGIFLSKSGIYTIEQRLEGFIEGCELLGIKINEEWIAKDLFPEEKTYDKLKKLK
KNHNLPTAIIATNNKITKQLISASKKLNILIPNTLSIMGYDDLTENEIISPPITCIKQPVFEIGRIASTILLGKIENDET
KTTKIVLKTEIVERNSIINLNKNTLN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=27.8443113772455, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=27.299703264095, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=26.3473053892216, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=29.3768545994065, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789202, Length=339, Percent_Identity=24.7787610619469, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787906, Length=340, Percent_Identity=23.5294117647059, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=25.2941176470588, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.3492063492064, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790715, Length=339, Percent_Identity=23.3038348082596, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=22.6299694189602, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786540, Length=352, Percent_Identity=23.0113636363636, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=22.4550898203593, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39058; Mature: 39058

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTTIKDIAKALNISPSTVSRALNGKNGVSEKLSVTIREKAEELGYVGNFAARSLKTSQ
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHEEHHHHHHHHCCCHHHHHHHHHCCCC
SKTIGVTVSDIRNPFFLDFLKGVDNILFQRGYKFLLTDSGENVEKEKIYLMWLIEHGVDG
CCEEEEEHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCCC
ILASPVTERDGKNNLELYKKIKNISIPIVFYDRVFPEESQFDSVTVDNKDSIIQALKYLY
EECCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHH
NKGHRKIGIFLSKSGIYTIEQRLEGFIEGCELLGIKINEEWIAKDLFPEEKTYDKLKKLK
HCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHHH
KNHNLPTAIIATNNKITKQLISASKKLNILIPNTLSIMGYDDLTENEIISPPITCIKQPV
HCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHH
FEIGRIASTILLGKIENDETKTTKIVLKTEIVERNSIINLNKNTLN
HHHHHHHHHHHEEECCCCCCCEEEEEEEEEEECCCCEEECCCCCCC
>Mature Secondary Structure
MQKTTIKDIAKALNISPSTVSRALNGKNGVSEKLSVTIREKAEELGYVGNFAARSLKTSQ
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHEEHHHHHHHHCCCHHHHHHHHHCCCC
SKTIGVTVSDIRNPFFLDFLKGVDNILFQRGYKFLLTDSGENVEKEKIYLMWLIEHGVDG
CCEEEEEHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCCC
ILASPVTERDGKNNLELYKKIKNISIPIVFYDRVFPEESQFDSVTVDNKDSIIQALKYLY
EECCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHH
NKGHRKIGIFLSKSGIYTIEQRLEGFIEGCELLGIKINEEWIAKDLFPEEKTYDKLKKLK
HCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHHH
KNHNLPTAIIATNNKITKQLISASKKLNILIPNTLSIMGYDDLTENEIISPPITCIKQPV
HCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHH
FEIGRIASTILLGKIENDETKTTKIVLKTEIVERNSIINLNKNTLN
HHHHHHHHHHHEEECCCCCCCEEEEEEEEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA