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The map label for this gene is trmB [H]

Identifier: 160902831

GI number: 160902831

Start: 1492012

End: 1492956

Strand: Direct

Name: trmB [H]

Synonym: Pmob_1385

Alternate gene names: 160902831

Gene position: 1492012-1492956 (Clockwise)

Preceding gene: 160902830

Following gene: 160902832

Centisome position: 68.77

GC content: 30.26

Gene sequence:

>945_bases
TTGAATTCGTATTCTTTTTTACAAAAGTCTCAAATAAATACAAAAGATGTAAAAAGTTATCCTATCGAATGGAATAAGGT
TTTTGGAAACAGTAATAAGTTAATAGTTGAAATTGGATTCGGTGGTGGAGAGTTTTTAGTAAATTTAGCTAAAGAGAACA
ACAAGTATAATTATATAGGAATAGAAACATCCCTAACTTCTTGTAGCAAAATAAAAAGCAAAATCTTTCAAAATAATTTA
AACAACATACAAATAATTCTTGAAGATGCAAAATTCGCTCTTAGGGAATTTTTTTGTGATAACTCTGTTAATAAAGTAAT
AGTTAATTTCCCATGCCCATGGCCAAAAAGCAAACATTCAAAAAACCGTCTTTTTGACGAAAAATTCATAGACACATTAT
CGTCGGTATTAGAAGTAGATGGTGAATTAACTTTAACAACCGACGATTTTGTCTATGCTTCTGACGTTGAAGAAAAATTC
ACAAGCAACGGATGCTTTGAAGTAAAAAATATTGCCGAAGTTCAGCAATTGCCTTTCAAAACTAGATATGAAAAAAAATG
GGAAAGTGAAGGTAGAAAAAAGTATTTATTAATAGCACGAAAAAGGTTCAAAAAAAACATAAAAAGATTGCTAGAAGGGG
AATTAGATTTGCCTCATGAGAAAATTAAGAAAGTAGATTTTGATAAATTACAATCTCTGGTTGGACTAAAAAAATCCATT
AGTAACATGGTTATAGTTATAAAAGATGTATACACTAAAATAGATGGTTTGGAATATTTAGTGAAGGTTTATTCTGAAGA
CGAGGGGTATATTCAAAGTTACTTTGTGAATGTCATCAGAATGAGAGAAGGATGGTTAGTAAAACTAGACGATATAGCAA
AAGCTTACAGAACCCCTTCTGTTAAAGAAGCTGTGAATACAATCGCCAAGGAGCTGGAAAATTAA

Upstream 100 bases:

>100_bases
TTAGGCGAAATGGCACCAGAAAGTTCACCAGTAAACGAGTGATCCATAAATTTTATAATCAAAAAGATTATAACCCCCTT
TTTTGGGAGGTATTGAAATT

Downstream 100 bases:

>100_bases
TGCCTCATATAACGGTTTTGGGTGCGGGTAGTTGGGGAACCGCTATATCGAAGCATTTAGTCGATAATGATCAAAAAGTA
ACAATTTGGGATAGGAACAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNSYSFLQKSQINTKDVKSYPIEWNKVFGNSNKLIVEIGFGGGEFLVNLAKENNKYNYIGIETSLTSCSKIKSKIFQNNL
NNIQIILEDAKFALREFFCDNSVNKVIVNFPCPWPKSKHSKNRLFDEKFIDTLSSVLEVDGELTLTTDDFVYASDVEEKF
TSNGCFEVKNIAEVQQLPFKTRYEKKWESEGRKKYLLIARKRFKKNIKRLLEGELDLPHEKIKKVDFDKLQSLVGLKKSI
SNMVIVIKDVYTKIDGLEYLVKVYSEDEGYIQSYFVNVIRMREGWLVKLDDIAKAYRTPSVKEAVNTIAKELEN

Sequences:

>Translated_314_residues
MNSYSFLQKSQINTKDVKSYPIEWNKVFGNSNKLIVEIGFGGGEFLVNLAKENNKYNYIGIETSLTSCSKIKSKIFQNNL
NNIQIILEDAKFALREFFCDNSVNKVIVNFPCPWPKSKHSKNRLFDEKFIDTLSSVLEVDGELTLTTDDFVYASDVEEKF
TSNGCFEVKNIAEVQQLPFKTRYEKKWESEGRKKYLLIARKRFKKNIKRLLEGELDLPHEKIKKVDFDKLQSLVGLKKSI
SNMVIVIKDVYTKIDGLEYLVKVYSEDEGYIQSYFVNVIRMREGWLVKLDDIAKAYRTPSVKEAVNTIAKELEN
>Mature_314_residues
MNSYSFLQKSQINTKDVKSYPIEWNKVFGNSNKLIVEIGFGGGEFLVNLAKENNKYNYIGIETSLTSCSKIKSKIFQNNL
NNIQIILEDAKFALREFFCDNSVNKVIVNFPCPWPKSKHSKNRLFDEKFIDTLSSVLEVDGELTLTTDDFVYASDVEEKF
TSNGCFEVKNIAEVQQLPFKTRYEKKWESEGRKKYLLIARKRFKKNIKRLLEGELDLPHEKIKKVDFDKLQSLVGLKKSI
SNMVIVIKDVYTKIDGLEYLVKVYSEDEGYIQSYFVNVIRMREGWLVKLDDIAKAYRTPSVKEAVNTIAKELEN

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=152, Percent_Identity=29.6052631578947, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 36390; Mature: 36390

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYSFLQKSQINTKDVKSYPIEWNKVFGNSNKLIVEIGFGGGEFLVNLAKENNKYNYIG
CCCHHHHHHHCCCHHHHHCCCCCCEEEECCCCEEEEEEECCCHHHHEEEECCCCCEEEEE
IETSLTSCSKIKSKIFQNNLNNIQIILEDAKFALREFFCDNSVNKVIVNFPCPWPKSKHS
EEHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
KNRLFDEKFIDTLSSVLEVDGELTLTTDDFVYASDVEEKFTSNGCFEVKNIAEVQQLPFK
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHHCCCCCEEHHHHHHHHHCCCH
TRYEKKWESEGRKKYLLIARKRFKKNIKRLLEGELDLPHEKIKKVDFDKLQSLVGLKKSI
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
SNMVIVIKDVYTKIDGLEYLVKVYSEDEGYIQSYFVNVIRMREGWLVKLDDIAKAYRTPS
CCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCC
VKEAVNTIAKELEN
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNSYSFLQKSQINTKDVKSYPIEWNKVFGNSNKLIVEIGFGGGEFLVNLAKENNKYNYIG
CCCHHHHHHHCCCHHHHHCCCCCCEEEECCCCEEEEEEECCCHHHHEEEECCCCCEEEEE
IETSLTSCSKIKSKIFQNNLNNIQIILEDAKFALREFFCDNSVNKVIVNFPCPWPKSKHS
EEHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
KNRLFDEKFIDTLSSVLEVDGELTLTTDDFVYASDVEEKFTSNGCFEVKNIAEVQQLPFK
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHHCCCCCEEHHHHHHHHHCCCH
TRYEKKWESEGRKKYLLIARKRFKKNIKRLLEGELDLPHEKIKKVDFDKLQSLVGLKKSI
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
SNMVIVIKDVYTKIDGLEYLVKVYSEDEGYIQSYFVNVIRMREGWLVKLDDIAKAYRTPS
CCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCC
VKEAVNTIAKELEN
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]