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The map label for this gene is metK

Identifier: 160902829

GI number: 160902829

Start: 1490368

End: 1491573

Strand: Direct

Name: metK

Synonym: Pmob_1383

Alternate gene names: 160902829

Gene position: 1490368-1491573 (Clockwise)

Preceding gene: 160902828

Following gene: 160902830

Centisome position: 68.69

GC content: 38.97

Gene sequence:

>1206_bases
GTGAAAAAAATGCTCTTTACCAGTGAAAGTGTAACTGAAGGTCACCCAGATAAAATCTGTGATCAAATTTCTGATACTAT
ACTTGATGCTATACTGGAAAAAGAACCTGAAGAAAATAAGATGAATGCCCGATGTGCTGTGGAAACTTTGGTGACAAGGG
GGCTAGTTGTAGTTACAGGTGAAGTTAGAACCTCAGCATATATAGATGTTCCAACGCTTGTCAGGAATACTATTTTAGAT
ATAGGTTATAACAGAGCTAAATTTGGATTTGATGGCGAAACATGCGCTGTAATTACTTCTATTGAAGAGCAATCACCTGA
TATAGCCCTGGGGGTTGACAGATCCTTCGAAGTTAAATCAAAAGAAAAGGAAGATCCTTTTGAGAAGATTGGAGCAGGAG
ACCAGGGCATTATGTTCGGATATGCCACAAACGAAACGGATGCTTATATGCCTTTACCCATTCTCTTAGCTCACAGACTT
GCAAAAAGATTGGCTGATGTTCGAAAATCGAACACCTTGGATTTTTTAAGGCCCGATGGTAAAACCCAAGTAACGGTAGA
ATACGATGAAAATAATAACCCGGTGGGCGTTGAAACTGTACTTATTTCTGCTCAGCATTCTCCTGATATAAGTAGACAAG
AGCTAGAAGAGGCAATAAAAGAACATGTAATAACCCCGGTGATACCCGAAAATCTTGTTACAAAAGACACAAAAATTCTT
GTCAATCCTACAGGAAGATTTGTGATTGGAGGTCCTCAAGCTGATACAGGTCTCACAGGAAGGAAGATAATCGTTGATAC
ATATGGTGGCTGGGCACCACACGGTGGAGGAGCCTTTTCAGGAAAAGATCCTACCAAAGTAGATAGATCCGCTACTTATA
TGGCAAGATATGTAGCCAAAAATTTGGTTGCAAGTGGTGCTGCAGATGAAGTTTTAATTCAACTGTCTTACGCCATTGGT
GTTGCTCAACCTGTTTCAATAAATATTGACACAAAAGGTACTGCAAAAGTGGACGAAGAAAAGATATATAAAGTTGTAAA
AGAGATTTTTGATTTTAGGCCTGCCGCTATAATTAGTAACTTGAATCTTTTACAACCTATTTACCAAAAGACTGCTGCTT
ACGGACATTTCGGGAGAAAAGATGTAGAATTTCCCTGGGAGAGATTGGATAAAGTTAAGGAATTGAAAGCAGCATTAGGT
TTGTAA

Upstream 100 bases:

>100_bases
ACTTGAGAAATTTGATTTTTTTGATATAATATAGCTCGTGGAATTCTAAAGATAATCTGTAATTCGTCGAAAAACTCGAA
GAAATTAAGGAGGCTAAAAA

Downstream 100 bases:

>100_bases
TTTACTAAAAGGCAAAAGATTTTGATTTTAAAAGGGTTACAGGACGAAAAATAAAAGTTGAGGAGGTATTTAAAAAATTG
CCAAATAAAAAATCAGCAGA

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKMLFTSESVTEGHPDKICDQISDTILDAILEKEPEENKMNARCAVETLVTRGLVVVTGEVRTSAYIDVPTLVRNTILD
IGYNRAKFGFDGETCAVITSIEEQSPDIALGVDRSFEVKSKEKEDPFEKIGAGDQGIMFGYATNETDAYMPLPILLAHRL
AKRLADVRKSNTLDFLRPDGKTQVTVEYDENNNPVGVETVLISAQHSPDISRQELEEAIKEHVITPVIPENLVTKDTKIL
VNPTGRFVIGGPQADTGLTGRKIIVDTYGGWAPHGGGAFSGKDPTKVDRSATYMARYVAKNLVASGAADEVLIQLSYAIG
VAQPVSINIDTKGTAKVDEEKIYKVVKEIFDFRPAAIISNLNLLQPIYQKTAAYGHFGRKDVEFPWERLDKVKELKAALG
L

Sequences:

>Translated_401_residues
MKKMLFTSESVTEGHPDKICDQISDTILDAILEKEPEENKMNARCAVETLVTRGLVVVTGEVRTSAYIDVPTLVRNTILD
IGYNRAKFGFDGETCAVITSIEEQSPDIALGVDRSFEVKSKEKEDPFEKIGAGDQGIMFGYATNETDAYMPLPILLAHRL
AKRLADVRKSNTLDFLRPDGKTQVTVEYDENNNPVGVETVLISAQHSPDISRQELEEAIKEHVITPVIPENLVTKDTKIL
VNPTGRFVIGGPQADTGLTGRKIIVDTYGGWAPHGGGAFSGKDPTKVDRSATYMARYVAKNLVASGAADEVLIQLSYAIG
VAQPVSINIDTKGTAKVDEEKIYKVVKEIFDFRPAAIISNLNLLQPIYQKTAAYGHFGRKDVEFPWERLDKVKELKAALG
L
>Mature_401_residues
MKKMLFTSESVTEGHPDKICDQISDTILDAILEKEPEENKMNARCAVETLVTRGLVVVTGEVRTSAYIDVPTLVRNTILD
IGYNRAKFGFDGETCAVITSIEEQSPDIALGVDRSFEVKSKEKEDPFEKIGAGDQGIMFGYATNETDAYMPLPILLAHRL
AKRLADVRKSNTLDFLRPDGKTQVTVEYDENNNPVGVETVLISAQHSPDISRQELEEAIKEHVITPVIPENLVTKDTKIL
VNPTGRFVIGGPQADTGLTGRKIIVDTYGGWAPHGGGAFSGKDPTKVDRSATYMARYVAKNLVASGAADEVLIQLSYAIG
VAQPVSINIDTKGTAKVDEEKIYKVVKEIFDFRPAAIISNLNLLQPIYQKTAAYGHFGRKDVEFPWERLDKVKELKAALG
L

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=387, Percent_Identity=55.0387596899225, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI5174529, Length=393, Percent_Identity=52.9262086513995, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1789311, Length=401, Percent_Identity=58.354114713217, Blast_Score=475, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25145633, Length=394, Percent_Identity=52.5380710659898, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=53.2133676092545, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=394, Percent_Identity=52.5380710659898, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17551082, Length=394, Percent_Identity=50.761421319797, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=48.586118251928, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI25145635, Length=342, Percent_Identity=51.7543859649123, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI32565911, Length=342, Percent_Identity=51.7543859649123, Blast_Score=331, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI32565903, Length=310, Percent_Identity=53.5483870967742, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI32565909, Length=88, Percent_Identity=48.8636363636364, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320710, Length=395, Percent_Identity=51.1392405063291, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323209, Length=388, Percent_Identity=51.8041237113402, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45552159, Length=387, Percent_Identity=50.9043927648579, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=387, Percent_Identity=50.9043927648579, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=387, Percent_Identity=50.9043927648579, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=387, Percent_Identity=50.9043927648579, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=387, Percent_Identity=50.9043927648579, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=387, Percent_Identity=50.9043927648579, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=387, Percent_Identity=50.6459948320413, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580525, Length=387, Percent_Identity=50.6459948320413, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=49.4652406417112, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580539, Length=132, Percent_Identity=46.969696969697, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PETMO (A9BHX2)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568410.1
- ProteinModelPortal:   A9BHX2
- SMR:   A9BHX2
- GeneID:   5756570
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1383
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- BioCyc:   PMOB403833:PMOB_1383-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44056; Mature: 44056

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMLFTSESVTEGHPDKICDQISDTILDAILEKEPEENKMNARCAVETLVTRGLVVVTG
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEE
EVRTSAYIDVPTLVRNTILDIGYNRAKFGFDGETCAVITSIEEQSPDIALGVDRSFEVKS
CCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHCCCCEEEECCCCCCCCC
KEKEDPFEKIGAGDQGIMFGYATNETDAYMPLPILLAHRLAKRLADVRKSNTLDFLRPDG
CCCCCCHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCC
KTQVTVEYDENNNPVGVETVLISAQHSPDISRQELEEAIKEHVITPVIPENLVTKDTKIL
CEEEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VNPTGRFVIGGPQADTGLTGRKIIVDTYGGWAPHGGGAFSGKDPTKVDRSATYMARYVAK
ECCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NLVASGAADEVLIQLSYAIGVAQPVSINIDTKGTAKVDEEKIYKVVKEIFDFRPAAIISN
HHHHCCCHHHHHEEEHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHC
LNLLQPIYQKTAAYGHFGRKDVEFPWERLDKVKELKAALGL
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKMLFTSESVTEGHPDKICDQISDTILDAILEKEPEENKMNARCAVETLVTRGLVVVTG
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEE
EVRTSAYIDVPTLVRNTILDIGYNRAKFGFDGETCAVITSIEEQSPDIALGVDRSFEVKS
CCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHCCCCEEEECCCCCCCCC
KEKEDPFEKIGAGDQGIMFGYATNETDAYMPLPILLAHRLAKRLADVRKSNTLDFLRPDG
CCCCCCHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCC
KTQVTVEYDENNNPVGVETVLISAQHSPDISRQELEEAIKEHVITPVIPENLVTKDTKIL
CEEEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VNPTGRFVIGGPQADTGLTGRKIIVDTYGGWAPHGGGAFSGKDPTKVDRSATYMARYVAK
ECCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
NLVASGAADEVLIQLSYAIGVAQPVSINIDTKGTAKVDEEKIYKVVKEIFDFRPAAIISN
HHHHCCCHHHHHEEEHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHC
LNLLQPIYQKTAAYGHFGRKDVEFPWERLDKVKELKAALGL
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA