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The map label for this gene is punA [H]

Identifier: 160902817

GI number: 160902817

Start: 1476766

End: 1477593

Strand: Direct

Name: punA [H]

Synonym: Pmob_1371

Alternate gene names: 160902817

Gene position: 1476766-1477593 (Clockwise)

Preceding gene: 160902816

Following gene: 160902818

Centisome position: 68.07

GC content: 38.53

Gene sequence:

>828_bases
ATGGATATTGAACAGTACGTATCAAAAGTGAGAGAAGCAGCTAAGTATATTCAAGAAAAAACCACGAAAAAGCCCAGAAT
TGCGATCGTTTTAGGGTCTGGATTAGGGAAAATTTCACAAAATTTAGAAGATGCCCTAGCAATCCCTTATTCTGATATTC
CTAACTTCCCCCGCTCTACCGCTCCAGGCCATAAAGGCGAATTAATGATAGGGAGTTTAAAGGGTAAAGATACGTTACTT
ATGAATGGAAGATTTCATTACTACGAAGGGTACACTATGAAAGAGGTTACCTTTCCAATACGTGTAATGCAAGAATTAGG
TATTGAAACTCTTGTATTGACTAACGCAGCAGGAACATTAAATCCTGATTTTGAAGTGGGTGTACCGTGTATAATCACGG
ATCACATCAACTTTTTTGGTGACAACCCCTTGATTGGACCAAATTTCGATGATTGGGGTCCTAGATTTCCAGATATGACG
GAAGTTTACTCGAAATCTTTGGTGCAAGAAGCCTTTAAATCTGCTAAAAAACTTAATATTAGAGTATATTCTGGTGTATA
TTTAGGTTTAAGCGGTCCTACATTTGAAACACCTGCTGAAATGGCTATGATGAGAAATTTTGGCGCCGATTTGGTCGGGA
TGTCGACTGTACCAGAGGCTATAGTTGCAAAACATGCTGGTATGGAGATTTTGGGTATCACCGCTATAACTGATAAAGCT
GTTCCTGAGCAATTGAAAGAAGTTAGTGCAGAAGAAGTTTTGAAGATTGCAGAAAAAACAGGCGAAAATATAGCTGAGAT
CATCATGGACCTAGTAGATATCTTTTAG

Upstream 100 bases:

>100_bases
AATTGGAGATAAATTTATAGTAGGGTACGGATTGGATTACAATGAAAAATATAGAAATTTACCTTACATTGGATATATAG
AATAAGGAGGCTAATATTAC

Downstream 100 bases:

>100_bases
AAGGTGATCTTTTGAAGTCTTTTGTTTTGTTGGCAATTTTTATATTTTTGCCAATTTTGTTCTTTTCTTATTATGAAGTT
TACGATTACTTTTTTATGAA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MDIEQYVSKVREAAKYIQEKTTKKPRIAIVLGSGLGKISQNLEDALAIPYSDIPNFPRSTAPGHKGELMIGSLKGKDTLL
MNGRFHYYEGYTMKEVTFPIRVMQELGIETLVLTNAAGTLNPDFEVGVPCIITDHINFFGDNPLIGPNFDDWGPRFPDMT
EVYSKSLVQEAFKSAKKLNIRVYSGVYLGLSGPTFETPAEMAMMRNFGADLVGMSTVPEAIVAKHAGMEILGITAITDKA
VPEQLKEVSAEEVLKIAEKTGENIAEIIMDLVDIF

Sequences:

>Translated_275_residues
MDIEQYVSKVREAAKYIQEKTTKKPRIAIVLGSGLGKISQNLEDALAIPYSDIPNFPRSTAPGHKGELMIGSLKGKDTLL
MNGRFHYYEGYTMKEVTFPIRVMQELGIETLVLTNAAGTLNPDFEVGVPCIITDHINFFGDNPLIGPNFDDWGPRFPDMT
EVYSKSLVQEAFKSAKKLNIRVYSGVYLGLSGPTFETPAEMAMMRNFGADLVGMSTVPEAIVAKHAGMEILGITAITDKA
VPEQLKEVSAEEVLKIAEKTGENIAEIIMDLVDIF
>Mature_275_residues
MDIEQYVSKVREAAKYIQEKTTKKPRIAIVLGSGLGKISQNLEDALAIPYSDIPNFPRSTAPGHKGELMIGSLKGKDTLL
MNGRFHYYEGYTMKEVTFPIRVMQELGIETLVLTNAAGTLNPDFEVGVPCIITDHINFFGDNPLIGPNFDDWGPRFPDMT
EVYSKSLVQEAFKSAKKLNIRVYSGVYLGLSGPTFETPAEMAMMRNFGADLVGMSTVPEAIVAKHAGMEILGITAITDKA
VPEQLKEVSAEEVLKIAEKTGENIAEIIMDLVDIF

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=265, Percent_Identity=44.5283018867925, Blast_Score=246, Evalue=1e-65,
Organism=Homo sapiens, GI47132622, Length=261, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788746, Length=251, Percent_Identity=39.8406374501992, Blast_Score=187, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17541190, Length=272, Percent_Identity=37.5, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323238, Length=260, Percent_Identity=43.8461538461538, Blast_Score=208, Evalue=8e-55,
Organism=Drosophila melanogaster, GI45552885, Length=272, Percent_Identity=40.0735294117647, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24656093, Length=272, Percent_Identity=40.0735294117647, Blast_Score=220, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24656090, Length=272, Percent_Identity=40.0735294117647, Blast_Score=220, Evalue=7e-58,
Organism=Drosophila melanogaster, GI45552887, Length=272, Percent_Identity=40.0735294117647, Blast_Score=220, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24762376, Length=244, Percent_Identity=33.1967213114754, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI221459247, Length=265, Percent_Identity=24.5283018867925, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 30253; Mature: 30253

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEQYVSKVREAAKYIQEKTTKKPRIAIVLGSGLGKISQNLEDALAIPYSDIPNFPRST
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCC
APGHKGELMIGSLKGKDTLLMNGRFHYYEGYTMKEVTFPIRVMQELGIETLVLTNAAGTL
CCCCCCCEEEEECCCCCEEEECCEEEEECCEEEHHHHHHHHHHHHCCCEEEEEECCCCCC
NPDFEVGVPCIITDHINFFGDNPLIGPNFDDWGPRFPDMTEVYSKSLVQEAFKSAKKLNI
CCCCCCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
RVYSGVYLGLSGPTFETPAEMAMMRNFGADLVGMSTVPEAIVAKHAGMEILGITAITDKA
EEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEHHHHH
VPEQLKEVSAEEVLKIAEKTGENIAEIIMDLVDIF
HHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MDIEQYVSKVREAAKYIQEKTTKKPRIAIVLGSGLGKISQNLEDALAIPYSDIPNFPRST
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCC
APGHKGELMIGSLKGKDTLLMNGRFHYYEGYTMKEVTFPIRVMQELGIETLVLTNAAGTL
CCCCCCCEEEEECCCCCEEEECCEEEEECCEEEHHHHHHHHHHHHCCCEEEEEECCCCCC
NPDFEVGVPCIITDHINFFGDNPLIGPNFDDWGPRFPDMTEVYSKSLVQEAFKSAKKLNI
CCCCCCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
RVYSGVYLGLSGPTFETPAEMAMMRNFGADLVGMSTVPEAIVAKHAGMEILGITAITDKA
EEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEHHHHH
VPEQLKEVSAEEVLKIAEKTGENIAEIIMDLVDIF
HHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]