Click here to switch to the map view.

The map label for this gene is mutB [H]

Identifier: 160902025

GI number: 160902025

Start: 603761

End: 605440

Strand: Direct

Name: mutB [H]

Synonym: Pmob_0549

Alternate gene names: 160902025

Gene position: 603761-605440 (Clockwise)

Preceding gene: 160902016

Following gene: 160902026

Centisome position: 27.83

GC content: 35.54

Gene sequence:

>1680_bases
ATGTTTGAAAGAGAAAAAATTCATGAAATAGAAAAGTCAAAAAAAGAATGGGAAGGGAAAAAATTAGAACCTACCTTAAG
ACGTTTCCCAGAGAGAAAAAAGGAATTTGTTACCTCATATGAAGATGAGATTGAAAGGCTTTATACACCTTTAGATATAG
AAAATTTAGATTACCTTAAAGATTTAGGCTTTCCTGGTGAATACCCTTTCACCAGAGGTGTACAACCAACCATGTACCGA
GGTAAGTTGTGGACCATGCGTCAATATGCAGGATTCGGGTCCGCAGAAGAATCCAACAAAAGATATAAATACTTATTAGA
ACAAGGCCAAACAGGTTTATCTATAGCCTTTGACCTGCCAACACAAATAGGTTTTGACTCAGACGATCCCATGTCCGAGG
GGGAAGTAGGAAAGGTTGGAGTAGCAATAGATTCACTAAAAGATATGGAGATTCTTTTCGACGGCATCCCTCTTGATAAG
GTGAGTGTTTCAATGACTATCAACTCAACTGCGATGATTTTATTGGCAATGTTAATGACTATTGCAAAAAAACAAGGTGT
TCCTTATGAAAAACTTAGAGGCACTATTCAGAACGATATTTTAAAAGAATACATAGCAAGGGGCACTTATATCTTTCCAC
CAAAAGAATCTATGAAGTTGATAGTAGATATTTTTGATTATGGAAGCAAAAACCTTCCAAAATTTAACTTAATAAGTATC
AGTGGCTATCATATTAGGGAAGCAGGAGCCAATGCTGTTCAAGAAGTAGCTTTCACATTGGCAGATGGAATAGCCTATGT
CGAGGCAGCAATTAAAGCCGGATTAGATCCAAATGAATTTGGAAGGAACCTTTCATTCTTTTTTAATGCCCATAACAACT
TTTTAGAAGAAGTGGCGAAATTTAGGGCAGCCAGAAAATTATGGGCGAAAATTATGAAAGAACGCTTCGGTGTGACGGAT
GAAGACGCAATGAAATTGAAATTTCATACCCAGACTGCCGGTTCCACATTAACCGCACAACAACCTTTGAATAACATAAT
AAGGGTAACAATTCAAGCTTTGGCTGCAGTTTTAGGTGGTACCCAATCACTTCACACTAATTCTTATGATGAGGCTTTAG
GACTGCCTACAGAACAATCCGTCACCGTCGCTTTAAGAACACAACAAATCATAGCCCACGAAATCGGAGTAAGCGAAACC
GTAGATCCTCTAGCGGGATCGTTCGCTATAGAAAAATTAACTTTAGACATAGAAAAAGAAGCTCAAAAATATTTAGAAAA
AATAGATGAGATAGGCGGAATGGTAAAAGCCATAGAGGACGGATACCCTCAAAAAGAAATATTAAATAGTGCTTATAAAA
CACAAAAAAGGATAGAAAATAAAGAACAGATCATAGTGGGGATTAATGAATACAATTCTGACAAAAAAGAAAAGATAGAT
ATCCTAAAAGTTGATGAAGAACAAGAAGAAAAGCAAATAGAAAAGTTAAAGAATTTAAAAAGGCAAAGAGATACCACAAA
AGTAGAACAATCTTTGAATAGATTGAAAAAGGCTGCAATAAACGAGGAGAATTTATTCCCTTATGTAATTGAATGTGTAG
AAAACTATTCAACAATAGGAGAAATAACAAAAATTTTAAAAGAAGTTTATGGGGAGTATCGTGAAAGTACTACAATCTAA

Upstream 100 bases:

>100_bases
ATTTGTAAATTTTAAAATAATAGGGTAAAATAATAATGACCCCGGGTAATGAAATTTTTTTCATAGTTCGTATAAAAATT
TATTGTGGGAGGAAATTCCT

Downstream 100 bases:

>100_bases
TTATTTTTTTATATTAACCGAACCTTTGCCATTTTAGAAACTTTATACAATGAGGGTGGGGAGCGGGGCAAAGGGGCGCT
GACAGAAATTTTAGAGTTTT

Product: methylmalonyl-CoA mutase, large subunit

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MFEREKIHEIEKSKKEWEGKKLEPTLRRFPERKKEFVTSYEDEIERLYTPLDIENLDYLKDLGFPGEYPFTRGVQPTMYR
GKLWTMRQYAGFGSAEESNKRYKYLLEQGQTGLSIAFDLPTQIGFDSDDPMSEGEVGKVGVAIDSLKDMEILFDGIPLDK
VSVSMTINSTAMILLAMLMTIAKKQGVPYEKLRGTIQNDILKEYIARGTYIFPPKESMKLIVDIFDYGSKNLPKFNLISI
SGYHIREAGANAVQEVAFTLADGIAYVEAAIKAGLDPNEFGRNLSFFFNAHNNFLEEVAKFRAARKLWAKIMKERFGVTD
EDAMKLKFHTQTAGSTLTAQQPLNNIIRVTIQALAAVLGGTQSLHTNSYDEALGLPTEQSVTVALRTQQIIAHEIGVSET
VDPLAGSFAIEKLTLDIEKEAQKYLEKIDEIGGMVKAIEDGYPQKEILNSAYKTQKRIENKEQIIVGINEYNSDKKEKID
ILKVDEEQEEKQIEKLKNLKRQRDTTKVEQSLNRLKKAAINEENLFPYVIECVENYSTIGEITKILKEVYGEYRESTTI

Sequences:

>Translated_559_residues
MFEREKIHEIEKSKKEWEGKKLEPTLRRFPERKKEFVTSYEDEIERLYTPLDIENLDYLKDLGFPGEYPFTRGVQPTMYR
GKLWTMRQYAGFGSAEESNKRYKYLLEQGQTGLSIAFDLPTQIGFDSDDPMSEGEVGKVGVAIDSLKDMEILFDGIPLDK
VSVSMTINSTAMILLAMLMTIAKKQGVPYEKLRGTIQNDILKEYIARGTYIFPPKESMKLIVDIFDYGSKNLPKFNLISI
SGYHIREAGANAVQEVAFTLADGIAYVEAAIKAGLDPNEFGRNLSFFFNAHNNFLEEVAKFRAARKLWAKIMKERFGVTD
EDAMKLKFHTQTAGSTLTAQQPLNNIIRVTIQALAAVLGGTQSLHTNSYDEALGLPTEQSVTVALRTQQIIAHEIGVSET
VDPLAGSFAIEKLTLDIEKEAQKYLEKIDEIGGMVKAIEDGYPQKEILNSAYKTQKRIENKEQIIVGINEYNSDKKEKID
ILKVDEEQEEKQIEKLKNLKRQRDTTKVEQSLNRLKKAAINEENLFPYVIECVENYSTIGEITKILKEVYGEYRESTTI
>Mature_559_residues
MFEREKIHEIEKSKKEWEGKKLEPTLRRFPERKKEFVTSYEDEIERLYTPLDIENLDYLKDLGFPGEYPFTRGVQPTMYR
GKLWTMRQYAGFGSAEESNKRYKYLLEQGQTGLSIAFDLPTQIGFDSDDPMSEGEVGKVGVAIDSLKDMEILFDGIPLDK
VSVSMTINSTAMILLAMLMTIAKKQGVPYEKLRGTIQNDILKEYIARGTYIFPPKESMKLIVDIFDYGSKNLPKFNLISI
SGYHIREAGANAVQEVAFTLADGIAYVEAAIKAGLDPNEFGRNLSFFFNAHNNFLEEVAKFRAARKLWAKIMKERFGVTD
EDAMKLKFHTQTAGSTLTAQQPLNNIIRVTIQALAAVLGGTQSLHTNSYDEALGLPTEQSVTVALRTQQIIAHEIGVSET
VDPLAGSFAIEKLTLDIEKEAQKYLEKIDEIGGMVKAIEDGYPQKEILNSAYKTQKRIENKEQIIVGINEYNSDKKEKID
ILKVDEEQEEKQIEKLKNLKRQRDTTKVEQSLNRLKKAAINEENLFPYVIECVENYSTIGEITKILKEVYGEYRESTTI

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=544, Percent_Identity=47.6102941176471, Blast_Score=509, Evalue=1e-144,
Organism=Escherichia coli, GI1789284, Length=511, Percent_Identity=50.0978473581213, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25144500, Length=511, Percent_Identity=48.53228962818, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 63542; Mature: 63542

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEREKIHEIEKSKKEWEGKKLEPTLRRFPERKKEFVTSYEDEIERLYTPLDIENLDYLK
CCCHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DLGFPGEYPFTRGVQPTMYRGKLWTMRQYAGFGSAEESNKRYKYLLEQGQTGLSIAFDLP
HCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC
TQIGFDSDDPMSEGEVGKVGVAIDSLKDMEILFDGIPLDKVSVSMTINSTAMILLAMLMT
HHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHH
IAKKQGVPYEKLRGTIQNDILKEYIARGTYIFPPKESMKLIVDIFDYGSKNLPKFNLISI
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCCCCCEEEEEE
SGYHIREAGANAVQEVAFTLADGIAYVEAAIKAGLDPNEFGRNLSFFFNAHNNFLEEVAK
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECHHHHHHHHHH
FRAARKLWAKIMKERFGVTDEDAMKLKFHTQTAGSTLTAQQPLNNIIRVTIQALAAVLGG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
TQSLHTNSYDEALGLPTEQSVTVALRTQQIIAHEIGVSETVDPLAGSFAIEKLTLDIEKE
CCCCCCCCCHHHCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AQKYLEKIDEIGGMVKAIEDGYPQKEILNSAYKTQKRIENKEQIIVGINEYNSDKKEKID
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC
ILKVDEEQEEKQIEKLKNLKRQRDTTKVEQSLNRLKKAAINEENLFPYVIECVENYSTIG
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EITKILKEVYGEYRESTTI
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFEREKIHEIEKSKKEWEGKKLEPTLRRFPERKKEFVTSYEDEIERLYTPLDIENLDYLK
CCCHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DLGFPGEYPFTRGVQPTMYRGKLWTMRQYAGFGSAEESNKRYKYLLEQGQTGLSIAFDLP
HCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECC
TQIGFDSDDPMSEGEVGKVGVAIDSLKDMEILFDGIPLDKVSVSMTINSTAMILLAMLMT
HHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHH
IAKKQGVPYEKLRGTIQNDILKEYIARGTYIFPPKESMKLIVDIFDYGSKNLPKFNLISI
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCCCCCEEEEEE
SGYHIREAGANAVQEVAFTLADGIAYVEAAIKAGLDPNEFGRNLSFFFNAHNNFLEEVAK
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECHHHHHHHHHH
FRAARKLWAKIMKERFGVTDEDAMKLKFHTQTAGSTLTAQQPLNNIIRVTIQALAAVLGG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
TQSLHTNSYDEALGLPTEQSVTVALRTQQIIAHEIGVSETVDPLAGSFAIEKLTLDIEKE
CCCCCCCCCHHHCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AQKYLEKIDEIGGMVKAIEDGYPQKEILNSAYKTQKRIENKEQIIVGINEYNSDKKEKID
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC
ILKVDEEQEEKQIEKLKNLKRQRDTTKVEQSLNRLKKAAINEENLFPYVIECVENYSTIG
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EITKILKEVYGEYRESTTI
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]