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The map label for this gene is nusA [H]

Identifier: 160902006

GI number: 160902006

Start: 576594

End: 577619

Strand: Direct

Name: nusA [H]

Synonym: Pmob_0530

Alternate gene names: 160902006

Gene position: 576594-577619 (Clockwise)

Preceding gene: 160902005

Following gene: 160902008

Centisome position: 26.58

GC content: 31.77

Gene sequence:

>1026_bases
TTGAACTTAAACCTATTAGAAGCTTTAGACGCCTTGGAAAGAGATAAAGGTATAAGTAGGGACTCGTTAGTTGATATTAT
AGCTAAATCTATTAAAAGTGCGTACAAAAAAAATTATGGAGCAAAAAACGTTGAAATTGAAATTGATAAAAATCTAACAA
AATTGAATGTTTATCAACTATGGAAAGTCGTTGAAACAGTTGAAAACCCTAAAGAAGAAATTTCTTTGGAAGAAGCTAAA
AAAATTAATCCAAAAATAGAAATAGGTGAAATGGTAAGAAAAAAGATCAATCTTAAAAAGGATTTTAGGCGAGTTGCCGC
TCAAACAGCTAAACAAGTGATACTTCAAAATTTGAAGGAATTGGAAAAGAAAAACTTGTTTGATAAATACGTAGCTTTAA
AAAATAGGATTTCAACCGCTGAAATAATTAAAGTTAGTGATGAATATATTGACATCAGAATAGGAAAATTAGAGACTAAA
CTCCCTATGAAAGAGACAATTCCTGGAGAAACTTTTAAAAGCGGAGAACTTGTAAAAGTCTACATAAAAAACATTCAAAA
CACATCAAAAGGCCCAAAAATTATGGTTTCAAGAACTGTACCTGAACTCATTACAGAACTACTATCATCTATCGTACCCG
AGATAGAAGAAGGTATCATAAAAATTGTAAAAATAGTCAGGGAACCCGGAATACGCACTAAAGTAGCTGTTGTCAGTTCC
ATGCCTGGAGTTGATCCTGTAGGTGCTTGTATCGGTGAAAAAGGTTCAAGAATCAGTGAGTTACTGAAAGAATTAAAAAA
TGAAAAAGTAGATATAATAGAATATTCAGAAGATCCTAAAATTTTTATAAAGCATGCTCTTGCCCCTGCTGAAGTAAAAA
ATATAATTCTTAACGAAGATGAAAAAACTGCTTTTGTTTACGTCCCAGAGAATCAACTTTCTTTAGCTGTGGGAAAGGGA
GGACAAACTGCTAGATTAGCAGCAAAGATTACTGGATGGAAAATTGATATTCACTCATTAAAATAA

Upstream 100 bases:

>100_bases
AAGATTCTGGAAAATTATTTAACATCGATTTAAACGAAATAGACAAAGCCAATTTAGAAATTGAATTGTAAAATTTGAAA
GATATGGGAGGTGTGGAACA

Downstream 100 bases:

>100_bases
GTGAAAAAAAATACCGCATCAGTCAAGTAGATGCGGTATTTTTTTAGGGGTGAGAAATACAAATAGGTCATAATTATTAC
TGTAAAAAAAAGGAAGGGGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNLNLLEALDALERDKGISRDSLVDIIAKSIKSAYKKNYGAKNVEIEIDKNLTKLNVYQLWKVVETVENPKEEISLEEAK
KINPKIEIGEMVRKKINLKKDFRRVAAQTAKQVILQNLKELEKKNLFDKYVALKNRISTAEIIKVSDEYIDIRIGKLETK
LPMKETIPGETFKSGELVKVYIKNIQNTSKGPKIMVSRTVPELITELLSSIVPEIEEGIIKIVKIVREPGIRTKVAVVSS
MPGVDPVGACIGEKGSRISELLKELKNEKVDIIEYSEDPKIFIKHALAPAEVKNIILNEDEKTAFVYVPENQLSLAVGKG
GQTARLAAKITGWKIDIHSLK

Sequences:

>Translated_341_residues
MNLNLLEALDALERDKGISRDSLVDIIAKSIKSAYKKNYGAKNVEIEIDKNLTKLNVYQLWKVVETVENPKEEISLEEAK
KINPKIEIGEMVRKKINLKKDFRRVAAQTAKQVILQNLKELEKKNLFDKYVALKNRISTAEIIKVSDEYIDIRIGKLETK
LPMKETIPGETFKSGELVKVYIKNIQNTSKGPKIMVSRTVPELITELLSSIVPEIEEGIIKIVKIVREPGIRTKVAVVSS
MPGVDPVGACIGEKGSRISELLKELKNEKVDIIEYSEDPKIFIKHALAPAEVKNIILNEDEKTAFVYVPENQLSLAVGKG
GQTARLAAKITGWKIDIHSLK
>Mature_341_residues
MNLNLLEALDALERDKGISRDSLVDIIAKSIKSAYKKNYGAKNVEIEIDKNLTKLNVYQLWKVVETVENPKEEISLEEAK
KINPKIEIGEMVRKKINLKKDFRRVAAQTAKQVILQNLKELEKKNLFDKYVALKNRISTAEIIKVSDEYIDIRIGKLETK
LPMKETIPGETFKSGELVKVYIKNIQNTSKGPKIMVSRTVPELITELLSSIVPEIEEGIIKIVKIVREPGIRTKVAVVSS
MPGVDPVGACIGEKGSRISELLKELKNEKVDIIEYSEDPKIFIKHALAPAEVKNIILNEDEKTAFVYVPENQLSLAVGKG
GQTARLAAKITGWKIDIHSLK

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=342, Percent_Identity=31.5789473684211, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 38292; Mature: 38292

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNLLEALDALERDKGISRDSLVDIIAKSIKSAYKKNYGAKNVEIEIDKNLTKLNVYQL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
WKVVETVENPKEEISLEEAKKINPKIEIGEMVRKKINLKKDFRRVAAQTAKQVILQNLKE
HHHHHHHHCCHHHCCHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LEKKNLFDKYVALKNRISTAEIIKVSDEYIDIRIGKLETKLPMKETIPGETFKSGELVKV
HHHHHHHHHHHHHHHCCCHHHEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCCCCHHH
YIKNIQNTSKGPKIMVSRTVPELITELLSSIVPEIEEGIIKIVKIVREPGIRTKVAVVSS
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHC
MPGVDPVGACIGEKGSRISELLKELKNEKVDIIEYSEDPKIFIKHALAPAEVKNIILNED
CCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCHHHHHHHHCCCC
EKTAFVYVPENQLSLAVGKGGQTARLAAKITGWKIDIHSLK
CCEEEEEECCCCEEEEECCCCCCEEEEEEEECEEEEEECCC
>Mature Secondary Structure
MNLNLLEALDALERDKGISRDSLVDIIAKSIKSAYKKNYGAKNVEIEIDKNLTKLNVYQL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
WKVVETVENPKEEISLEEAKKINPKIEIGEMVRKKINLKKDFRRVAAQTAKQVILQNLKE
HHHHHHHHCCHHHCCHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LEKKNLFDKYVALKNRISTAEIIKVSDEYIDIRIGKLETKLPMKETIPGETFKSGELVKV
HHHHHHHHHHHHHHHCCCHHHEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCCCCHHH
YIKNIQNTSKGPKIMVSRTVPELITELLSSIVPEIEEGIIKIVKIVREPGIRTKVAVVSS
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHC
MPGVDPVGACIGEKGSRISELLKELKNEKVDIIEYSEDPKIFIKHALAPAEVKNIILNED
CCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCHHHHHHHHCCCC
EKTAFVYVPENQLSLAVGKGGQTARLAAKITGWKIDIHSLK
CCEEEEEECCCCEEEEECCCCCCEEEEEEEECEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]