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The map label for this gene is aceF [H]

Identifier: 160877157

GI number: 160877157

Start: 4782964

End: 4784961

Strand: Reverse

Name: aceF [H]

Synonym: Sbal195_4053

Alternate gene names: 160877157

Gene position: 4784961-4782964 (Counterclockwise)

Preceding gene: 160877158

Following gene: 160877156

Centisome position: 89.48

GC content: 50.95

Gene sequence:

>1998_bases
ATGGCTGAATTAAAAGAAGTTTTTGTTCCTGATATCGGCGGCGATGAAGTGCAAGTGATCGAAATTTGTGTGGCTGTGGG
TGATACCCTAGCGGCAGAAGAATCGATTCTTACCGTCGAAAGCGACAAGGCGACTATGGATATTCCAGCGCCTTTCGCCG
GTGTTTTAGCCGAATTGAAAGTGGCGGTGGGCGATAAAGTATCTGAAGGTACTTTGATTGCTATGATGCAAGCGGCGGGC
GCGGCAGCTGCCGCTCCGGCTCCAGTTGCAGCACCCGCTCAGGCGGCACCAGCATCTGCACCAGTTCAAGCGGCTCCAGC
CCCAGTGGCTGCTGCTCCTGCGACGGGCGCAACCAAAGTGGTTGAAGTGACTGTACCTGATATCGGTGGCGACACTGATG
TATCGGTTATCGAAGTCCTGGTTGCTGTCGGTGACAAGATTGAAGTTGATAGCGGTTTAATTACCCTAGAAACAGACAAA
GCGACGATGGACGTGCCATCACCTTTCGCGGGTGTGGTTAAAGACGTTAAAGTCGCAGTTGGCGATAAAGTGTCGCAAGG
CTCTTTAGTCATCATGCTTGAAGTTGGCGGCGCTGCACCTGCAGCCGCGCCAACGGTTGCTGCCCAAGCGGCTCCTGCCG
CTATGGTAGCGCCAGTGGCCGCAGCACCTGCTGCACCAGCGGCAAGTGTGGTTGCGGTAAAAGAAATTCAAGTGCCTGAT
ATTGGCGATGCCAGCAATGTCGATGTGATCGAAGTGCTCGTGTCTGTCGGTGATGAAATCACTGCTGACCAAGGTTTGAT
TACCCTTGAAACTGACAAAGCCACCATGGAAGTGCCTGCGCCATTCGCCGGTAAACTGCTGTCTTTGACTGTTAAAGTGG
GTGACAAGGTTTCTCAAGGTAGCGTTATTGCAACGATTGAAACTGTCACAGTGGGTGCAGCTCCAGCAGCGGTTGCTCAA
GCGGCAGCTCCTGCTCCAGTGAGCGCGGCGCCCGTTGCAGCCCCAACACCAGCAAGCCGTCCTCCAGTGCCACACCACCC
AAGCGCAGGTACGCCTTTGTCTACCGGTGCGGTACATGCGTCTCCTGCGGTACGTCGTTTAGCCCGTGAATTTGGTGTCG
ACTTGACCCAAGTTGCTGGTACTGGCCGTAAAGGTCGCATTATGAGAGAAGACGTTCAGGCGTATGTGAAATACGAACTG
TCTCGTCCGAAAGCAACTGCGGCAACCTCTGTGGGTTCAGGTAACGGCGGCGGTCTGCAAGTTATTGCAGCACCTAAAGT
TGATTTCAGTAAGTTTGGTGAAGTGGAAGAAATTCCATTAAGCCGTATCCAGAAGATTTCTGGCCCTAACTTACACCGCA
ACTGGGTAACTATTCCGCACGTGACTCAGTTCGATGAAGCTGATATCACTGAAATGGAAGAGTTCCGTAAGCAGCAAAAC
GATGTAGCGGCGAAGAAGAAAGCCGATTACAAGATCACGCCTTTAGTGTTTATGCTAAAAGCTGTGGCTAAAACGTTGCA
ACAGTTCCCAGTGTTCAACTCAAGCTTAAGTAGCGATGGCGAATCACTGATCCAGAAGAAGTATTTCCATATCGGTGTGG
CGGTTGATACGCCAAACGGTTTGGTTGTGCCAGTCGTGCGTGACGTGGATAAGAAAGGCATTATCGAGTTATCTCGTGAA
CTGGCTGATATCTCTATCCGTGCCCGTGATGGCAAGCTGAAATCTGCTGACATGCAAGGTAGCTGTTTTACTATTTCTAG
TTTAGGTGGCATTGGCGGTACAGCGTTTACGCCTATCGTTAACTACCCAGACGTGGCGATTTTAGGTGTGTCTAAATCTG
AAATTAAGCCTAAGTGGAATGGTAAAGAGTTCGAACCTAAATTGATGTTGCCATTGTCGCTGTCATACGATCACCGTGTG
ATCGATGGTGCTATGGCTGCACGCTTTAGTGTGACGCTGTCAGGAATTCTGTCCGATATTCGTACTTTGGTTCTGTAA

Upstream 100 bases:

>100_bases
TCGCAAAGAGCTGCCTGTCGATGTGTTAGCAAAAGCCATTAAAGAATATGGTATCGACGCTGACAAGATCAATCCACAGT
ACGCGTAAGAGGCAATGAAA

Downstream 100 bases:

>100_bases
GCATATAAGGCTGCTCATCTTGAGTGGCCTTTTGTTTATTGTGATCAGTATCAAACACAGGTTAAAATGCGCCCACCTTA
CGCGCTGGCGCATTTTCGGT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MAELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
AAAAAPAPVAAPAQAAPASAPVQAAPAPVAAAPATGATKVVEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDK
ATMDVPSPFAGVVKDVKVAVGDKVSQGSLVIMLEVGGAAPAAAPTVAAQAAPAAMVAPVAAAPAAPAASVVAVKEIQVPD
IGDASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTVGAAPAAVAQ
AAAPAPVSAAPVAAPTPASRPPVPHHPSAGTPLSTGAVHASPAVRRLAREFGVDLTQVAGTGRKGRIMREDVQAYVKYEL
SRPKATAATSVGSGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQN
DVAAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIELSRE
LADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRV
IDGAMAARFSVTLSGILSDIRTLVL

Sequences:

>Translated_665_residues
MAELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
AAAAAPAPVAAPAQAAPASAPVQAAPAPVAAAPATGATKVVEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDK
ATMDVPSPFAGVVKDVKVAVGDKVSQGSLVIMLEVGGAAPAAAPTVAAQAAPAAMVAPVAAAPAAPAASVVAVKEIQVPD
IGDASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTVGAAPAAVAQ
AAAPAPVSAAPVAAPTPASRPPVPHHPSAGTPLSTGAVHASPAVRRLAREFGVDLTQVAGTGRKGRIMREDVQAYVKYEL
SRPKATAATSVGSGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQN
DVAAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIELSRE
LADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRV
IDGAMAARFSVTLSGILSDIRTLVL
>Mature_664_residues
AELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAGA
AAAAPAPVAAPAQAAPASAPVQAAPAPVAAAPATGATKVVEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKA
TMDVPSPFAGVVKDVKVAVGDKVSQGSLVIMLEVGGAAPAAAPTVAAQAAPAAMVAPVAAAPAAPAASVVAVKEIQVPDI
GDASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTVGAAPAAVAQA
AAPAPVSAAPVAAPTPASRPPVPHHPSAGTPLSTGAVHASPAVRRLAREFGVDLTQVAGTGRKGRIMREDVQAYVKYELS
RPKATAATSVGSGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQND
VAAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIELSREL
ADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVI
DGAMAARFSVTLSGILSDIRTLVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=27.6497695852535, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI31711992, Length=328, Percent_Identity=32.9268292682927, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI203098816, Length=441, Percent_Identity=28.7981859410431, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.3612334801762, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI203098753, Length=441, Percent_Identity=28.7981859410431, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=36.0544217687075, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786305, Length=661, Percent_Identity=56.1270801815431, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=409, Percent_Identity=30.5623471882641, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=28.9411764705882, Blast_Score=159, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=30.715935334873, Blast_Score=145, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=214, Percent_Identity=38.785046728972, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=322, Percent_Identity=30.4347826086957, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=453, Percent_Identity=29.8013245033113, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=430, Percent_Identity=25.8139534883721, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=28.7703016241299, Blast_Score=167, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=35.5140186915888, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=29.7872340425532, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=29.7872340425532, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 68207; Mature: 68076

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCCHHHHCCCCCCCCCHHHHHHHHHCCCHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGAAAAAPAPVAAPAQAAPASAPVQAAPAPVAAAPATGATKV
HHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKATMDVPSPFAGVVKDVKVAV
EEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
GDKVSQGSLVIMLEVGGAAPAAAPTVAAQAAPAAMVAPVAAAPAAPAASVVAVKEIQVPD
CCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEECCCC
IGDASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQG
CCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEEEEEECCCCCCC
SVIATIETVTVGAAPAAVAQAAAPAPVSAAPVAAPTPASRPPVPHHPSAGTPLSTGAVHA
CEEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
SPAVRRLAREFGVDLTQVAGTGRKGRIMREDVQAYVKYELSRPKATAATSVGSGNGGGLQ
CHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEE
VIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQN
EEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHH
DVAAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNG
HHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEEEECCCC
LVVPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIV
EEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
NYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGILSDI
CCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RTLVL
HHHCC
>Mature Secondary Structure 
AELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCHHHHCCCCCCCCCHHHHHHHHHCCCHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGAAAAAPAPVAAPAQAAPASAPVQAAPAPVAAAPATGATKV
HHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKATMDVPSPFAGVVKDVKVAV
EEEEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
GDKVSQGSLVIMLEVGGAAPAAAPTVAAQAAPAAMVAPVAAAPAAPAASVVAVKEIQVPD
CCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEECCCC
IGDASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQG
CCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEEEEEECCCCCCC
SVIATIETVTVGAAPAAVAQAAAPAPVSAAPVAAPTPASRPPVPHHPSAGTPLSTGAVHA
CEEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
SPAVRRLAREFGVDLTQVAGTGRKGRIMREDVQAYVKYELSRPKATAATSVGSGNGGGLQ
CHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEE
VIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQN
EEEECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHH
DVAAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNG
HHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEEEECCCC
LVVPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIV
EEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
NYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGILSDI
CCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RTLVL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]