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The map label for this gene is lpdA [H]

Identifier: 160877156

GI number: 160877156

Start: 4781389

End: 4782816

Strand: Reverse

Name: lpdA [H]

Synonym: Sbal195_4052

Alternate gene names: 160877156

Gene position: 4782816-4781389 (Counterclockwise)

Preceding gene: 160877157

Following gene: 160877155

Centisome position: 89.44

GC content: 46.5

Gene sequence:

>1428_bases
ATGAGTAACGAAATCAAAACTCAGGTAGTGGTATTAGGTGCAGGTCCTGCAGGTTATTCTGCGGCTTTCCGTGCAGCGGA
CTTAGGTCTAGAAACCATTATCGTTGAACGTTTTAGCACTTTAGGCGGCGTGTGTCTTAATGTGGGTTGTATCCCATCTA
AAGCCCTATTACATGTTGCTAAAGTTATCGAAGAAGCCAAAGCCGTTGCTGCTCACGGTGTGGTTTTCGGCGAGCCAACT
ATCGATTTAGATAAGTTACGTGGCTTTAAAGAAAAAGTCATTGGCCAATTAACTGGCGGATTGGGCGGTATGTCCAAAAT
GCGTAAAGTTAACGTGGTTAACGGTTTTGGTAAATTCACTGGCCCTAACACGCTAGAAGTGACTGCTGAAGATGGCACTG
TCAAAGTGGTTCAATTCGAGCAAGCTATCATTGCTGCGGGTTCTCGCCCAATCAAACTGCCATTCATTCCGCATGAAGAT
CCACGTATTTGGGATTCGACTGACGCATTAGAACTGAAAGAAGTTCCAGGCAAATTGTTAGTGATGGGCGGCGGTATTAT
CGGCCTAGAAATGGGTACTGTGTACGCTTCTTTAGGCAGCGAGATCGACGTGGTTGAAATGTTCGACCAAGTGATCCCAG
CGGCTGATAAAGACGTTGTTCGCGTATTCACTAAGCAAATCAAGAAGAAATTCAACCTGATCCTTGAAACTAAAGTCACA
GCGGTAGAAGCCCGTGAAGACGGTATCTATGTTTCTATGGAAGGTAAGAGCGCACCAACTGAAGCAGTCCGTTACGATGC
CGTATTGGTAGCAATTGGCCGTGCACCTAATGGCAAGTCGTTAGATGCTGAAAAAGCAGGTGTTAACGTTGATGAGCGTG
GCTTTATCAAGGTTGATAAGCAACTGCGTACTAACGTACCGCACATCTATGCAATTGGTGACATCGTTGGTCAACCTATG
TTGGCTCACAAAGGCGTGCACGAAGGCCACGTAGCGGCTGAAGTTATCGCTGGCATGAAGCACTACTTCGATCCAAAAGT
GATCCCATCAATTGCTTACACTGACCCAGAAGTAGCTTGGGTTGGTTTAACTGAGAAAGAAGCGAAAGAGCAAGGTATTG
CTTACGAAACCGCAACTTTCCCATGGGCAGCAAGTGGCCGCGCTATCGCATCTGATGCGAGCGAAGGCATGACTAAGCTG
ATTTTCGACAAAGACACTCATCGCGTAATCGGTGGTGCGATTGTTGGCGTTAACGGTGGCGAGCTGTTAGGCGAAATCGG
TTTAGCGATTGAAATGGGTTGTGATGCTGAAGATTTAGCATTAACCATTCACGCTCACCCAACACTGCACGAATCAGTGG
GCTTAGCCGCTGAAATGTACGAAGGTTCTATTACTGATTTGCCAAACCCAAAGGCAAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
GTATCAAACACAGGTTAAAATGCGCCCACCTTACGCGCTGGCGCATTTTCGGTTGGCAGGATAGCTCCTCCAACGGATTG
AATGAGAATTAGAGGAAAAC

Downstream 100 bases:

>100_bases
TTTAGCTGTCGATTAAAAAAGCGCTCATTTGAGCGCTTTTTTATTGCCTGTATTTTTTTGTAACTTTTATGTAAACAAGC
GGATGAATTGATAGGATTGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRGFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK

Sequences:

>Translated_475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRGFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
>Mature_474_residues
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPTI
DLDKLRGFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHEDP
RIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVTA
VEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDKQLRTNVPHIYAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKLI
FDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=42.7312775330396, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=27.6923076923077, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI148277071, Length=429, Percent_Identity=30.0699300699301, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI148277065, Length=429, Percent_Identity=30.0699300699301, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI33519430, Length=429, Percent_Identity=30.0699300699301, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33519428, Length=429, Percent_Identity=30.0699300699301, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33519426, Length=429, Percent_Identity=30.0699300699301, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=28.9351851851852, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=28.6026200873362, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=28.3372365339578, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=85.6842105263158, Blast_Score=833, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=27.3504273504274, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=28.7280701754386, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=28.6681715575621, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=40.3083700440529, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=28.9029535864979, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=444, Percent_Identity=27.2522522522523, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=444, Percent_Identity=27.2522522522523, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=451, Percent_Identity=26.8292682926829, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.6666666666667, Blast_Score=315, Evalue=9e-87,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=27.1367521367521, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=454, Percent_Identity=27.0925110132159, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=41.1378555798687, Blast_Score=344, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24640553, Length=463, Percent_Identity=30.2375809935205, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24640549, Length=463, Percent_Identity=30.2375809935205, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24640551, Length=463, Percent_Identity=30.2375809935205, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=27.2151898734177, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50465; Mature: 50334

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRGFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLL
CCCEEEEEECCCEEEEEEEEEHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEE
VMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDK
EEEECCCCEEEEECCCCCCCHHHEEEEEEEEEECCCCCCCCCHHHCCCCCCCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCEECCCHHCCCEEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRGFKEKVIGQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLL
CCCEEEEEECCCEEEEEEEEEHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEE
VMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDK
EEEECCCCEEEEECCCCCCCHHHEEEEEEEEEECCCCCCCCCHHHCCCCCCCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCEECCCHHCCCEEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]