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The map label for this gene is dmlR [H]

Identifier: 160876916

GI number: 160876916

Start: 4491986

End: 4492888

Strand: Direct

Name: dmlR [H]

Synonym: Sbal195_3812

Alternate gene names: 160876916

Gene position: 4491986-4492888 (Clockwise)

Preceding gene: 160876914

Following gene: 160876922

Centisome position: 84.01

GC content: 51.94

Gene sequence:

>903_bases
ATGGATAAAATTGCCGCAATACGCAGTTTCGTCGAAGTGGCAAACTGTGGCAGCTTTACCAAAGCTGCCGAGCATTTAGA
CCTCAGCCGCCTGCAGGTGTCCCGCCATATTCAAGAAGTGGAGCAGTGGTTGAGTTTACGCTTACTACACCGTACCACCC
GCAGCGTCAGCCTGACTTTGCAAGGCGAAGAAGCGCTGCAATTTTGCCAGCGGATATTAAGTGAAGTCGCCGCCATGGAA
AGCCGCGCCCACAGCCATAACAGTGAACTGGTGGGCAGTATTCGTATCGCCACACCAATTGGCCTTGGACAGCACAGCCT
GTTTGATGTTGTCGATCGCTTTATTAAAATTCACCCTAAGGTCAGCATTCAGCTGGTGATGTCAGACAGTTTTGCCCAAT
TGGTGGATGAGCGAGTCGATGTGGCGCTGCGCTACACCGAGCAGCCCGATGAGAATTTGATTGCCCGCCGTTTAATGACC
ATAGATGCCGTGATCTGCGCTGCGCCGAGTTACCTTGATAGCAACACACCACTTGTCGAGCCCATCGATCTACTGGCTCA
CAACTGCCTGATACACTCGGCGCAACAAAATTGGCCTTTCCTGAAAGAGCTGCAAGCCGAGCATGTAAAGGTCACAGGTA
ATCTTAAAGCCAACGATATGGGCGTATTGGTGAGTGCGGCGCTGCGGGGACATGGCATAGTGCGTTTGCCCTGTGACTTA
GCCAATCAATACCTTGCCAGTGGCCAGCTCAAAGAAGTGTTACCCAACTTTACGACGCCAGGCCAAACCCTGTGGGCAGT
GTACTTGTCCCGCAGTTATCAGCAAAATCTGGTGCGCGCCTTTATCGATTTCACTGCCCAGCAATGGCAACTCGATATCA
AAAAATGGCTCCCTGCGCATTAG

Upstream 100 bases:

>100_bases
AACTCTCTCTGATGATTTACATTGAAGCCAGTTTACTTGCAGTGGTTCCCGTGATAAACATCAATAAATGAGCATGATTG
TTTCAGTTTGAGGTACTTAT

Downstream 100 bases:

>100_bases
CATAAAAAACGCACTTAGGCGTTAGCGTAAGTGCGTGTTAATTGATGCTGAATCTGGGCAGATATCGAACGCTTATTCGT
CGAATTGCAACATAAAATCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDKIAAIRSFVEVANCGSFTKAAEHLDLSRLQVSRHIQEVEQWLSLRLLHRTTRSVSLTLQGEEALQFCQRILSEVAAME
SRAHSHNSELVGSIRIATPIGLGQHSLFDVVDRFIKIHPKVSIQLVMSDSFAQLVDERVDVALRYTEQPDENLIARRLMT
IDAVICAAPSYLDSNTPLVEPIDLLAHNCLIHSAQQNWPFLKELQAEHVKVTGNLKANDMGVLVSAALRGHGIVRLPCDL
ANQYLASGQLKEVLPNFTTPGQTLWAVYLSRSYQQNLVRAFIDFTAQQWQLDIKKWLPAH

Sequences:

>Translated_300_residues
MDKIAAIRSFVEVANCGSFTKAAEHLDLSRLQVSRHIQEVEQWLSLRLLHRTTRSVSLTLQGEEALQFCQRILSEVAAME
SRAHSHNSELVGSIRIATPIGLGQHSLFDVVDRFIKIHPKVSIQLVMSDSFAQLVDERVDVALRYTEQPDENLIARRLMT
IDAVICAAPSYLDSNTPLVEPIDLLAHNCLIHSAQQNWPFLKELQAEHVKVTGNLKANDMGVLVSAALRGHGIVRLPCDL
ANQYLASGQLKEVLPNFTTPGQTLWAVYLSRSYQQNLVRAFIDFTAQQWQLDIKKWLPAH
>Mature_300_residues
MDKIAAIRSFVEVANCGSFTKAAEHLDLSRLQVSRHIQEVEQWLSLRLLHRTTRSVSLTLQGEEALQFCQRILSEVAAME
SRAHSHNSELVGSIRIATPIGLGQHSLFDVVDRFIKIHPKVSIQLVMSDSFAQLVDERVDVALRYTEQPDENLIARRLMT
IDAVICAAPSYLDSNTPLVEPIDLLAHNCLIHSAQQNWPFLKELQAEHVKVTGNLKANDMGVLVSAALRGHGIVRLPCDL
ANQYLASGQLKEVLPNFTTPGQTLWAVYLSRSYQQNLVRAFIDFTAQQWQLDIKKWLPAH

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=32.5174825174825, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=33.8028169014084, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=29.6666666666667, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789639, Length=276, Percent_Identity=29.7101449275362, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=31.2714776632302, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=27.7397260273973, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787128, Length=281, Percent_Identity=27.7580071174377, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789173, Length=247, Percent_Identity=24.2914979757085, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787601, Length=144, Percent_Identity=31.9444444444444, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786448, Length=262, Percent_Identity=26.7175572519084, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=22.108843537415, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790011, Length=287, Percent_Identity=24.390243902439, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=31.9327731092437, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33718; Mature: 33718

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIAAIRSFVEVANCGSFTKAAEHLDLSRLQVSRHIQEVEQWLSLRLLHRTTRSVSLTL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
QGEEALQFCQRILSEVAAMESRAHSHNSELVGSIRIATPIGLGQHSLFDVVDRFIKIHPK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
VSIQLVMSDSFAQLVDERVDVALRYTEQPDENLIARRLMTIDAVICAAPSYLDSNTPLVE
EEEEEEECHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PIDLLAHNCLIHSAQQNWPFLKELQAEHVKVTGNLKANDMGVLVSAALRGHGIVRLPCDL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCEEEECHHH
ANQYLASGQLKEVLPNFTTPGQTLWAVYLSRSYQQNLVRAFIDFTAQQWQLDIKKWLPAH
HHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKIAAIRSFVEVANCGSFTKAAEHLDLSRLQVSRHIQEVEQWLSLRLLHRTTRSVSLTL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
QGEEALQFCQRILSEVAAMESRAHSHNSELVGSIRIATPIGLGQHSLFDVVDRFIKIHPK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
VSIQLVMSDSFAQLVDERVDVALRYTEQPDENLIARRLMTIDAVICAAPSYLDSNTPLVE
EEEEEEECHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PIDLLAHNCLIHSAQQNWPFLKELQAEHVKVTGNLKANDMGVLVSAALRGHGIVRLPCDL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCEEEECHHH
ANQYLASGQLKEVLPNFTTPGQTLWAVYLSRSYQQNLVRAFIDFTAQQWQLDIKKWLPAH
HHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]