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The map label for this gene is braB [H]

Identifier: 160876750

GI number: 160876750

Start: 4295428

End: 4296840

Strand: Reverse

Name: braB [H]

Synonym: Sbal195_3644

Alternate gene names: 160876750

Gene position: 4296840-4295428 (Counterclockwise)

Preceding gene: 160876757

Following gene: 160876749

Centisome position: 80.36

GC content: 47.2

Gene sequence:

>1413_bases
ATGGGGCTTCCAGTGCAAGACAATCAGATGAGTATCGCAGATACATTGGGCTTAGGTTTTATGACCTTCGCATTCTTTTT
AGGGGCAGGTAACCTAATTTTTCCACCGTTCGCGGGAATGTTAGCGGGCGGTAATATGCCCCTCGCCATGTTGGGTTTTT
TGATTACCGCCGTAGGTTTACCTTTAGTTGGACTGATTGCTGTCGCTAAGGCTCAAGGTAAGGTTATGGCTATGCTGCCA
GTATTTGCAGCAACGACATTGGCTATCGCGATTTATATCATTATCGGCCCTGCGTTTGCTGCGCCGCGTACTGGTTTAGT
GGCTTACGAGATTGGCGCTAAGCCCTTTATCGATGACACCACGGCCACATTTTTACTGGGCAGTTTAGAACTGAACCTGT
CGCAACTGCTATACACCATGTGTTTCTTCACTGTGACTATGTTATTGGCGCTTTTCCCTGGCAAGCTGCTCGATAGCGTA
GGTAAAGTATTAACGCCTATTTTATTGATCTTGTTAGTTGGATTGGCTGCATCTGTGATGTTTTTACCCGCATCCGAAGT
GGGCCAAGCCGTAGGCGATTATCAAAACCATCCGCTGACCAAAGGTATTTTGGAAGGCTACAACACTATGGATACGTTAG
CGTCCTTGATGTTTGGTATGCTGATTATCGATATCCTGCGCAAAAAGGGGATTGATCAACCCAGTGCTCAAACTAAATAT
CTGATCCGCGCCGCCTTTATTGCTGCCGCTGGTTTAGCCTTTGTGTATGTGTCGCTGTTCTTTTTAGGTGCGACCGCAGG
CGATATCGCTATCGGTGCTAAAAATGGCGGCGAGATCTTAACAAACTATGTGACCCGCGAATTTGGTGATTTAGGCTCAG
TCTTACTGTCATCGGTCGTGACTTTAGCTTGTTTAACCACAGCGGTTGGCCTTGTGAGTGCATGTTCTGAGTTCTTTAAT
GAGCTGATGCCTAAACTGTCTTACCGTTTCCTAGTAATCTTCTTAAGCGTGATTTGTGCTGTGATTGCTAACGTGGGTCT
TACGCAGCTTATCGCCATCAGTATTCCTGTGTTGATGACGATTTATCCTGTGGCAATCGCTTTAGTGGTCATCACTTTCT
TGACCGCTTATTTTGCAAATCCGCAGTTTGCACACCGTGCAGTACTGAGCGTGGCACTGTTTTTCGGCATCTTCGATGGC
CTTAAAGTGGCGGCGAGCAGTTTGACTGGGGCAGATGGTCAAGCGGTGAATGGCTTAGCGCAGGGCTTTATCCATGTCGT
GGATGTGATGTCTCCCGTGTTATCTAAGCTGCCACTCTATGAAGAAGGCATGGCTTGGTTACTGCCAACGATTGCAACCA
TAGTACTGTGTTTAGTGTTTGGTCGTGGCCGTGATCAAACGGCAGCTGCTTAA

Upstream 100 bases:

>100_bases
GAATTGTGTAAACAAAGATTTGCACTCCGGATGGGCTTTGTTTTAGCTATGGCTAATCGACACATAATCACTTAAGTTTG
TAAAAAAACAATAAGACAAG

Downstream 100 bases:

>100_bases
GCCTTGTACTCGCGTTTTTACGCGGGACTCAGTTAAGCTGATGCAGGCTTAAATGTAGTTAAGTTAATATCAACGGCGTA
TCATTTGATGACGCCGTTTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MGLPVQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLP
VFAATTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSV
GKVLTPILLILLVGLAASVMFLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKY
LIRAAFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLACLTTAVGLVSACSEFFN
ELMPKLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDG
LKVAASSLTGADGQAVNGLAQGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA

Sequences:

>Translated_470_residues
MGLPVQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLP
VFAATTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSV
GKVLTPILLILLVGLAASVMFLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKY
LIRAAFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLACLTTAVGLVSACSEFFN
ELMPKLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDG
LKVAASSLTGADGQAVNGLAQGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA
>Mature_469_residues
GLPVQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLPV
FAATTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVG
KVLTPILLILLVGLAASVMFLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKYL
IRAAFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLACLTTAVGLVSACSEFFNE
LMPKLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGL
KVAASSLTGADGQAVNGLAQGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=468, Percent_Identity=39.5299145299145, Blast_Score=332, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 49399; Mature: 49268

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLPVQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
PLVGLIAVAKAQGKVMAMLPVFAATTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEECCCCCCCCCH
TATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVGKVLTPILLILLVGLAASVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKY
HCCHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LIRAAFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLACLTTAVGLVSACSEFFNELMPKLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGLKVAASSLTGADGQAVNGLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
QGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA
HHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GLPVQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
PLVGLIAVAKAQGKVMAMLPVFAATTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEECCCCCCCCCH
TATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVGKVLTPILLILLVGLAASVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKY
HCCHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LIRAAFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLACLTTAVGLVSACSEFFNELMPKLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGLKVAASSLTGADGQAVNGLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
QGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA
HHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]