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The map label for this gene is ldhA [H]

Identifier: 160876735

GI number: 160876735

Start: 4277526

End: 4278515

Strand: Direct

Name: ldhA [H]

Synonym: Sbal195_3629

Alternate gene names: 160876735

Gene position: 4277526-4278515 (Clockwise)

Preceding gene: 160876734

Following gene: 160876736

Centisome position: 79.99

GC content: 48.28

Gene sequence:

>990_bases
ATGAGAATTGGATTCTTTAGCGCAAAACATTACGACATGCAGCATTTTAACCGCACAAATGCCGCGTTTGGTGCACAAAT
TGAGTATTTCGATTATCGCCTCTGCATGCAAACAGTGAAACTTGCTGAAGGGTTTGAAATCGTTTGTGCCTTCGTCAACG
ATTCACTCTGTGAAGAAGTCCTTGTCGAACTCGCCAAGGGCGGCACAAAAATTATCGCCATGCGCTGCGCAGGCTTTAAC
AATGTCGATTTAGTCGCCGCTAAACGCTTAGGCATGCAAGTCGTCAACGTGCCCGCTTATTCCCCAGAATCCGTTGCCGA
ACACACAGTCGCGCTGATGTTGACGCTGAATCGCAAAATCCACAAAGCCTACCAACGCACCCGCGATGCCAACTTCTCCC
TCGAAGGTTTAGTCGGTTTTAATATGTTCGGCAAAACCGTCGGCGTAATTGGTACGGGTAAAATCGGCGTGGCCACCATT
AAAGTCTTGCTCGGTTTTGGCTGTAAAGTCATCGCCTACGATCCCTTTATCAATCCAGCGGTTGTCGCGCTGAATGTGGA
ATATCAAGATCTCGACGCGATTTATGCCAACAGCGATATCATCAGCTTGCATTGCCCGCTCACGGCCGATAACCGCCATT
TGCTCAACCAAGCCAGCTTCGCCAAGATGAAACCCGGCGTCATGGTGATCAACACAAGCCGCGGTGGCTTACTCAATGCC
TTCGATGCGATGGAAGCCTTAAAACTCGGCCAAATAGGTTCACTTGGACTCGATGTGTACGAAAACGAAAAAGGCTTATT
CTTCGAAGACAAATCGAATGAGATCATTCAAGACGATGTATTCCGTCGATTATCTGCCTGCCATAACGTGATTTTTACCG
GTCACCAAGCCTTCCTCACCGAAGAAGCCTTAGGCGCCATTGCCCAAACAACCTTGAGCAATGTAAAAGCGCTATGTGCA
GGGGAACGCTCAGGTAACGAATTGTTTTAA

Upstream 100 bases:

>100_bases
ACTCTTTTAATAGCCAATTTTCTAAGGCTGAGGACAAAAAGCCCTAACTCACTTACACTCTTTGGCTGATAACTACATCA
AAATCCGTTAGGTGACAGAA

Downstream 100 bases:

>100_bases
CTGACAGATAAAATCCTGACTGCTAAAGTGAGGCTTCAACGTTTGAAGCCTCACAGAGAGAACTAAATATGTTAGTCACT
CAGGAAACTCAGGATATCTC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEVLVELAKGGTKIIAMRCAGFN
NVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATI
KVLLGFGCKVIAYDPFINPAVVALNVEYQDLDAIYANSDIISLHCPLTADNRHLLNQASFAKMKPGVMVINTSRGGLLNA
FDAMEALKLGQIGSLGLDVYENEKGLFFEDKSNEIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAQTTLSNVKALCA
GERSGNELF

Sequences:

>Translated_329_residues
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEVLVELAKGGTKIIAMRCAGFN
NVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATI
KVLLGFGCKVIAYDPFINPAVVALNVEYQDLDAIYANSDIISLHCPLTADNRHLLNQASFAKMKPGVMVINTSRGGLLNA
FDAMEALKLGQIGSLGLDVYENEKGLFFEDKSNEIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAQTTLSNVKALCA
GERSGNELF
>Mature_329_residues
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEVLVELAKGGTKIIAMRCAGFN
NVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATI
KVLLGFGCKVIAYDPFINPAVVALNVEYQDLDAIYANSDIISLHCPLTADNRHLLNQASFAKMKPGVMVINTSRGGLLNA
FDAMEALKLGQIGSLGLDVYENEKGLFFEDKSNEIIQDDVFRRLSACHNVIFTGHQAFLTEEALGAIAQTTLSNVKALCA
GERSGNELF

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=196, Percent_Identity=35.7142857142857, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI61743967, Length=243, Percent_Identity=32.9218106995885, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI4557497, Length=243, Percent_Identity=32.9218106995885, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI145580578, Length=262, Percent_Identity=30.9160305343511, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4557499, Length=262, Percent_Identity=30.9160305343511, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI145580575, Length=244, Percent_Identity=32.7868852459016, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI6912396, Length=249, Percent_Identity=28.5140562248996, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=61.890243902439, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI87082289, Length=195, Percent_Identity=30.2564102564103, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789279, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=210, Percent_Identity=32.8571428571429, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=233, Percent_Identity=33.9055793991416, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=234, Percent_Identity=31.6239316239316, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=199, Percent_Identity=32.1608040201005, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=234, Percent_Identity=31.1965811965812, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=27.9411764705882, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.1428571428571, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646446, Length=262, Percent_Identity=31.6793893129771, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24646448, Length=262, Percent_Identity=31.6793893129771, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24646452, Length=262, Percent_Identity=31.6793893129771, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24646450, Length=262, Percent_Identity=31.6793893129771, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI62472511, Length=262, Percent_Identity=32.0610687022901, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19921140, Length=238, Percent_Identity=28.9915966386555, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574286, Length=278, Percent_Identity=29.4964028776978, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45552429, Length=259, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24585514, Length=259, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28574282, Length=259, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28574284, Length=259, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45551003, Length=259, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571528, Length=292, Percent_Identity=26.7123287671233, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585516, Length=208, Percent_Identity=24.0384615384615, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36027; Mature: 36027

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEV
CEEEEECCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHH
LVELAKGGTKIIAMRCAGFNNVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHEEEHHHHHH
HKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATIKVLLGFGCKVIAYDPFINPA
HHHHHHHCCCCCEEEEEEEHHHHCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCE
VVALNVEYQDLDAIYANSDIISLHCPLTADNRHLLNQASFAKMKPGVMVINTSRGGLLNA
EEEEEEEHHHCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHH
FDAMEALKLGQIGSLGLDVYENEKGLFFEDKSNEIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHEECCCHHHHH
EEALGAIAQTTLSNVKALCAGERSGNELF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEV
CEEEEECCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHH
LVELAKGGTKIIAMRCAGFNNVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHEEEHHHHHH
HKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATIKVLLGFGCKVIAYDPFINPA
HHHHHHHCCCCCEEEEEEEHHHHCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCE
VVALNVEYQDLDAIYANSDIISLHCPLTADNRHLLNQASFAKMKPGVMVINTSRGGLLNA
EEEEEEEHHHCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHH
FDAMEALKLGQIGSLGLDVYENEKGLFFEDKSNEIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHEECCCHHHHH
EEALGAIAQTTLSNVKALCAGERSGNELF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]