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The map label for this gene is mdeA [H]

Identifier: 160876677

GI number: 160876677

Start: 4198649

End: 4199947

Strand: Reverse

Name: mdeA [H]

Synonym: Sbal195_3571

Alternate gene names: 160876677

Gene position: 4199947-4198649 (Counterclockwise)

Preceding gene: 160876678

Following gene: 160876674

Centisome position: 78.54

GC content: 50.65

Gene sequence:

>1299_bases
ATGAAGCTTGAATCACTGGCATTACACTACGGTTATGAGTCAGAAGCGACCACAAAAGCGGCCGCGGTACCTATCTATCA
AACCACCTCTTATACCTTCGATGATACCCAGCATGGTGCTGATTTATTTGACTTAAAAGTCGCAGGTAACATTTATACGC
GGATCATGAATCCTACTACTAGCGTGCTAGAGCAGCGTCTCGCAGCTATAGAAGGCGGCATAGGTGCACTGGCCGTGGCA
TCGGGTATGGCGGCGATTACCTATGCGATTCAAGCCCTGACTCAAGTTGGCGATAATATTGTCAGCACCAGTCAGCTCTA
TGGCGGCACTTATAATCTGTTTGCCCATACGCTGCCGCGCCAAGGCGTTGAAGTGCGTATGGCGGCCTTCGATGATTTTG
AAGGCCTCGATGAGCTGATCGACGACAAAACTAAGGCCTTGTTCTGCGAATCAATTGGTAATCCCGCGGGCAATATTGTC
GATTTACAGCGTTTAGCCGAGATTGCCCATAAGCATGGCGTACCTTTAATTGTCGATAATACGGTCGCAACCCCAGTGTT
ATGTCGCCCGTTTGAGCATGGCGCCGATATCGTTATCCACTCTTTAACCAAATACATAGGTGGCCATGGCACGACGATTG
GCGGGGTGATTATCGATTCAGGCAAGTTTGATTGGGTCGCCAATAAGGAAAGATTTGCCTTACTCAACCAAGCCGATCCT
TCTTACCATGGCGTGGTTTATACCGAAGCCTTTGGCCCTGCGGCGTTTATTGGTCGTTGCCGTGTGGTACCGCTACGTAA
TACCGGCGCCGCGCTTTCACCGCACAGCGCATTCTTACTGTTGCAAGGCCTTGAAACTCTAAGCCTTAGAATGGAGCGCC
ATTGCTCAAATGCGCTAGCATTAGCCGAATATTTGGTGTTGCATCCGGCGGTGAGTTGGGTGAACTACGGCGCGTTGCCA
AGCAGCCCTTATCAGGATAATTGCCACAAGATCACTGGCGGAAAAGCCTCTGGGATCATTAGCTTTGGGATTAAAGCGGC
CACGCCTGAAGAAGGTAAAATTGCCGGTGGACGTTTTATCGATGCGCTGCAGATGATCCTGCGTTTAGTGAACATCGGCG
ATGCGAAATCCTTAGCCTGTCACCCTGCGAGTACCACGCACAGACAGCTCGATGCCGATGAACTGGCAAGGGCGGGCGTG
TCAGAAGATCTTATCCGTATCTCGGTTGGGATTGAGCATATCGACGACATCATTAATGATGTGGCGCAGGCGCTCGATAA
GGCACTGGTGAGCAAATAG

Upstream 100 bases:

>100_bases
AGACTTCCAGATGTCTATCTGTTAGCTTGTTGAACATGCACAAATCTTGTGCAATCCTTTGGGTTCGAACAAGGATAGCA
CTCTGTTGAAGGAAGGCAAA

Downstream 100 bases:

>100_bases
TTCTTATTGAATAGCCCATATCGAATAGCTGATACCCCATCGATTTATTAAATCGTTAACAGTAAAAAGCCGTCATTGAT
TTAATTCGATGGCGGCTTTT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTTSVLEQRLAAIEGGIGALAVA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALALAEYLVLHPAVSWVNYGALP
SSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGV
SEDLIRISVGIEHIDDIINDVAQALDKALVSK

Sequences:

>Translated_432_residues
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTTSVLEQRLAAIEGGIGALAVA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALALAEYLVLHPAVSWVNYGALP
SSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGV
SEDLIRISVGIEHIDDIINDVAQALDKALVSK
>Mature_432_residues
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTTSVLEQRLAAIEGGIGALAVA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALALAEYLVLHPAVSWVNYGALP
SSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGV
SEDLIRISVGIEHIDDIINDVAQALDKALVSK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=32.5471698113208, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI299473759, Length=424, Percent_Identity=29.0094339622642, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25453487, Length=423, Percent_Identity=28.8416075650118, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1790375, Length=430, Percent_Identity=30.6976744186047, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=25.5813953488372, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=33.4939759036145, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=429, Percent_Identity=34.032634032634, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=429, Percent_Identity=33.5664335664336, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=47.945205479452, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=33.5064935064935, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=449, Percent_Identity=26.5033407572383, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=311, Percent_Identity=29.2604501607717, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=26.5588914549654, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922554, Length=432, Percent_Identity=32.4074074074074, Blast_Score=174, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46249; Mature: 46249

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTT
CCCHHEEEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHCCCHH
SVLEQRLAAIEGGIGALAVASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIVDLQRLAEIAHKHGVPLIVDN
CCCEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCEEECCCCEEEEECCCC
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALA
CCCEEEEECCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLVLHPAVSWVNYGALPSSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECEEECCCCCCCCCCHHHH
DALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGVSEDLIRISVGIEHIDDIIND
HHHHHHHHHHCCCCCCCEEECCCCCCHHCCCHHHHHHCCCCHHHEEEECCHHHHHHHHHH
VAQALDKALVSK
HHHHHHHHHHCC
>Mature Secondary Structure
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTT
CCCHHEEEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHCCCHH
SVLEQRLAAIEGGIGALAVASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIVDLQRLAEIAHKHGVPLIVDN
CCCEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCEEECCCCEEEEECCCC
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALA
CCCEEEEECCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLVLHPAVSWVNYGALPSSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECEEECCCCCCCCCCHHHH
DALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGVSEDLIRISVGIEHIDDIIND
HHHHHHHHHHCCCCCCCEEECCCCCCHHCCCHHHHHHCCCCHHHEEEECCHHHHHHHHHH
VAQALDKALVSK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]