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The map label for this gene is proB [H]

Identifier: 160876648

GI number: 160876648

Start: 4163909

End: 4165027

Strand: Reverse

Name: proB [H]

Synonym: Sbal195_3542

Alternate gene names: 160876648

Gene position: 4165027-4163909 (Counterclockwise)

Preceding gene: 160876649

Following gene: 160876647

Centisome position: 77.89

GC content: 51.12

Gene sequence:

>1119_bases
ATGAACTTAAGTGAGATTGCGTATCGCCGCGTAGTAGTGAAATTGGGGACGAGTGTGCTGACCTCTGGCAGTAAACAGCT
AGATAAAGCCCACATGGTTGAGCTTGCTCGTCAAATGGCCGCACTGATGAAGTCAGGGGTCGAAGTCGTATTATGCACCT
CTGGGGCCATTGCCGCTGGGCGTGAACATCTCCAATACCCGACACTGCCCGACACTATGGCCAATAAGCAATTGTTAGCC
GCTGTCGGGCAAAGTCAGTTGATCCTCGCTTGGTCACAACTCTTTAGTATTTATGGGCTGCATGTCGGCCAATTGCTGCT
GACCCGTGCCGATTTGCAGGATAGGGAGCGTTACCTTAACGCCCGCGACACGCTTAATGCCTTGCTGGCGAATAATATTG
TGCCGATCATCAATGAAAACGATGCCGTTGCCACTAATGAAATCAAAGTCGGTGATAACGACAATCTGTCGGCCCGTGCC
GCACTGCTGTGTGATGCTGACTTATTGATTTTATTGACGGATCAAAAGGGCTTATTTGATGCCGATCCGCGCACGAACCC
GAATGCTAAGTTGATCTCCCAAGTCGTCAAGATTGACGACAGCCTACGTATGTTGGCGGGCGGGTCTGTTTCTGGTTTAG
GTACGGGCGGAATGTCGACTAAATTGGAAGCGGCGGATATTGCTCGCCGTGCAGGCATCGAAGTGGTTATCGCCTCTGGC
CATCACCCAGATGTGATTAAAAAAGTGGTGAGCAAGGAATCCGTTGGCACGCATTTTAGTGCCATCGAAAACCCGCTCGA
AAGCCGTAAACAGTGGATTTTAGCCGGCCCTGCGACTCAAGGTTCTTTAGTGCTTGATGCGGGCGCAGTCAAAGCCGTGA
CAGACAAGGGCCGCAGCCTATTGTCCAAAGGTATCGTCGGCGTTAAAGGCCAGTTTGAGCGCGGCGCAACGCTGCAATTA
GTTGATCAACAAGGTAAAGTGATTGCTAAAGGGATGACACGATACTGCGGCCAAGCCCTCAGTTTGATCGCGGGCAAGCA
TTCCGACGAGATCGAGTCTTTACTCGGTTACGATTATGGTGACGCAATCGTGCACCGCAACGACATGGTTGTTTTATAA

Upstream 100 bases:

>100_bases
AAGCAAAATCGAAAATTTATCCCGAGTTAATAACTCCTTGCTACCAATTACCCAGCGGATTAGGTATAAGGATTGTTCGG
TTATTTTTTACAAATGAATA

Downstream 100 bases:

>100_bases
GCGAGGTCTGTTTTGAGTCAAATTAATCAAGATCAGTATTTACAGCAACTTGGTAATAATGCCAAACAGGCAAGTTATGC
CTTAGCTAACTTAACGGCGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMKSGVEVVLCTSGAIAAGREHLQYPTLPDTMANKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLQDRERYLNARDTLNALLANNIVPIINENDAVATNEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRTNPNAKLISQVVKIDDSLRMLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
HHPDVIKKVVSKESVGTHFSAIENPLESRKQWILAGPATQGSLVLDAGAVKAVTDKGRSLLSKGIVGVKGQFERGATLQL
VDQQGKVIAKGMTRYCGQALSLIAGKHSDEIESLLGYDYGDAIVHRNDMVVL

Sequences:

>Translated_372_residues
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMKSGVEVVLCTSGAIAAGREHLQYPTLPDTMANKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLQDRERYLNARDTLNALLANNIVPIINENDAVATNEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRTNPNAKLISQVVKIDDSLRMLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
HHPDVIKKVVSKESVGTHFSAIENPLESRKQWILAGPATQGSLVLDAGAVKAVTDKGRSLLSKGIVGVKGQFERGATLQL
VDQQGKVIAKGMTRYCGQALSLIAGKHSDEIESLLGYDYGDAIVHRNDMVVL
>Mature_372_residues
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMKSGVEVVLCTSGAIAAGREHLQYPTLPDTMANKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLQDRERYLNARDTLNALLANNIVPIINENDAVATNEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRTNPNAKLISQVVKIDDSLRMLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
HHPDVIKKVVSKESVGTHFSAIENPLESRKQWILAGPATQGSLVLDAGAVKAVTDKGRSLLSKGIVGVKGQFERGATLQL
VDQQGKVIAKGMTRYCGQALSLIAGKHSDEIESLLGYDYGDAIVHRNDMVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=35.8108108108108, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=35.7142857142857, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=63.3333333333333, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=34.4569288389513, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=34.4569288389513, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=35.1807228915663, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=411, Percent_Identity=33.8199513381995, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI21357643, Length=317, Percent_Identity=33.7539432176656, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39692; Mature: 39692

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMKSGVEVVLCTSGAIAAG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHC
REHLQYPTLPDTMANKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLQDRERYLN
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
ARDTLNALLANNIVPIINENDAVATNEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRTNPNAKLISQVVKIDDSLRMLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
CCCCCCCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECC
HHPDVIKKVVSKESVGTHFSAIENPLESRKQWILAGPATQGSLVLDAGAVKAVTDKGRSL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCHHHHHHHHHHH
LSKGIVGVKGQFERGATLQLVDQQGKVIAKGMTRYCGQALSLIAGKHSDEIESLLGYDYG
HHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
DAIVHRNDMVVL
CCEEECCCEEEC
>Mature Secondary Structure
MNLSEIAYRRVVVKLGTSVLTSGSKQLDKAHMVELARQMAALMKSGVEVVLCTSGAIAAG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHC
REHLQYPTLPDTMANKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLQDRERYLN
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
ARDTLNALLANNIVPIINENDAVATNEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRTNPNAKLISQVVKIDDSLRMLAGGSVSGLGTGGMSTKLEAADIARRAGIEVVIASG
CCCCCCCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECC
HHPDVIKKVVSKESVGTHFSAIENPLESRKQWILAGPATQGSLVLDAGAVKAVTDKGRSL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCHHHHHHHHHHH
LSKGIVGVKGQFERGATLQLVDQQGKVIAKGMTRYCGQALSLIAGKHSDEIESLLGYDYG
HHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
DAIVHRNDMVVL
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]