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The map label for this gene is proA [H]

Identifier: 160876647

GI number: 160876647

Start: 4162619

End: 4163896

Strand: Reverse

Name: proA [H]

Synonym: Sbal195_3541

Alternate gene names: 160876647

Gene position: 4163896-4162619 (Counterclockwise)

Preceding gene: 160876648

Following gene: 160876643

Centisome position: 77.87

GC content: 49.61

Gene sequence:

>1278_bases
TTGAGTCAAATTAATCAAGATCAGTATTTACAGCAACTTGGTAATAATGCCAAACAGGCAAGTTATGCCTTAGCTAACTT
AACGGCGAGCCAAAAGGCCGGTTTGCTTGAAGCCATCGCCGATGCGCTGACGGAAAACGCCGCCGCCATTTTAGCCGCGA
ATGCTAAGGATGTTGCCGCTGCTAAGGCCGATGGATTAACGGATGCGATGATCGACAGATTGTTGCTCGATGATGCGCGT
CTGGCGAGCATTATTGGCGATATTAGCGATGTGATCCGCCTTGCTGACCCAGTAGGCGAAGAATTTGGCAGTCAATTATT
AGATAACGGTTTAAGGCTGACTCGCCGCCGAGTACCACTTGGGGTTATCGGGGTGATTTACGAAGCTCGGCCCAATGTGA
CTGTTGATATCGCGGTACTTGCCCTTAAAACCGGTAATGCGGTGATCCTCCGTGGCGGTAAAGAAACCCTAGCATCGAAT
AAACTCATTAGTGACGTGATCCGCGGCGCGATTGTCGCTCAAGGTTTACCGATAGATGCGGTGCAATTTATTGATTCTTC
AGACAGAGCTTTAGTGACAGGTTTACTTAAGCTCGATCAATATGTCGACATGATAGTGCCCCGTGGCGGTCAAGCACTGC
AGCGCTTATGTGCTGAGCAGGCGACCATTCCGGTGATCTTAGGCGGCATTGGTATTTGTCATTTATATGTCGATAAAAAT
GCCAACCTTGAGCGTGCCTTAGCCGTGATCGCTAACGCCAAAGTGCAGCGTCCAACTGTATGTAATGCGCTTGATACCTT
GCTGGTGGATGAGCGCGTGGCGAGTACATTTGTGCCGCAAATTGCCGAGTATCTACATCGTTTAGGCGTGCGTTTTTCAG
TTTGTGACACAAGCTTTAGTCTGCTTGATGGTTTAGGGTTTGATGTGACGCTCGCCAAGGCTGAGGATTTTGGCATCGAA
TGGTTGTCGTTAACCTTAGGCATTAAAGTGGTGAGCGATATCGATGCCGCCGTTAGTCATATTCGCCAATATTCTAGCGG
CCATTCGGAAGCGATTTTGACCGATGACATTCACGCTGCGGCGAACTTTATGAACGAAGTGAACTCGGCCGCCGTGTATG
TGAATGCCAGCACGCGTTTTACCGATGGCGGTCAGTTTGGTCTTGGCGCTGAAGTGGCTGTGAGCACGCAAAAACTGCAC
GCCCGCGGCCCAATGGGGCTTGAGGCATTAACCACATATAAGTGGTTAGCTTGGGGTGATTACACTAGCCGTGTTTAG

Upstream 100 bases:

>100_bases
GGGCAAGCATTCCGACGAGATCGAGTCTTTACTCGGTTACGATTATGGTGACGCAATCGTGCACCGCAACGACATGGTTG
TTTTATAAGCGAGGTCTGTT

Downstream 100 bases:

>100_bases
CGGTTAAAAACCATAAACTTAAAGCCGTTAACACACTGTGTTGACGGTTTTTTTGTTTGCAGTGTTTGATTGTAAGTGCC
AATTTGCAGAGTCTATTTAT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAAAKADGLTDAMIDRLLLDDAR
LASIIGDISDVIRLADPVGEEFGSQLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASN
KLISDVIRGAIVAQGLPIDAVQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFSLLDGLGFDVTLAKAEDFGIE
WLSLTLGIKVVSDIDAAVSHIRQYSSGHSEAILTDDIHAAANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
ARGPMGLEALTTYKWLAWGDYTSRV

Sequences:

>Translated_425_residues
MSQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAAAKADGLTDAMIDRLLLDDAR
LASIIGDISDVIRLADPVGEEFGSQLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASN
KLISDVIRGAIVAQGLPIDAVQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFSLLDGLGFDVTLAKAEDFGIE
WLSLTLGIKVVSDIDAAVSHIRQYSSGHSEAILTDDIHAAANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
ARGPMGLEALTTYKWLAWGDYTSRV
>Mature_424_residues
SQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAAAKADGLTDAMIDRLLLDDARL
ASIIGDISDVIRLADPVGEEFGSQLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASNK
LISDVIRGAIVAQGLPIDAVQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKNA
NLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFSLLDGLGFDVTLAKAEDFGIEW
LSLTLGIKVVSDIDAAVSHIRQYSSGHSEAILTDDIHAAANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHA
RGPMGLEALTTYKWLAWGDYTSRV

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=36.319612590799, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=36.319612590799, Blast_Score=247, Evalue=1e-65,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=58.3132530120482, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=33.4939759036145, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=33.4939759036145, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324899, Length=431, Percent_Identity=37.8190255220418, Blast_Score=279, Evalue=7e-76,
Organism=Drosophila melanogaster, GI21357643, Length=410, Percent_Identity=36.5853658536585, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45265; Mature: 45134

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAA
CCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
AKADGLTDAMIDRLLLDDARLASIIGDISDVIRLADPVGEEFGSQLLDNGLRLTRRRVPL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCE
GVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASNKLISDVIRGAIVAQGLPIDA
EEEEEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCC
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFS
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHH
LLDGLGFDVTLAKAEDFGIEWLSLTLGIKVVSDIDAAVSHIRQYSSGHSEAILTDDIHAA
HHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
ANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGD
HHHHHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHEEECC
YTSRV
HHCCC
>Mature Secondary Structure 
SQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAA
CCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
AKADGLTDAMIDRLLLDDARLASIIGDISDVIRLADPVGEEFGSQLLDNGLRLTRRRVPL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCE
GVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASNKLISDVIRGAIVAQGLPIDA
EEEEEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCC
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFS
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHH
LLDGLGFDVTLAKAEDFGIEWLSLTLGIKVVSDIDAAVSHIRQYSSGHSEAILTDDIHAA
HHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
ANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGD
HHHHHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHEEECC
YTSRV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]