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The map label for this gene is rumA [H]

Identifier: 160876396

GI number: 160876396

Start: 3879575

End: 3880927

Strand: Reverse

Name: rumA [H]

Synonym: Sbal195_3290

Alternate gene names: 160876396

Gene position: 3880927-3879575 (Counterclockwise)

Preceding gene: 160876400

Following gene: 160876395

Centisome position: 72.58

GC content: 49.52

Gene sequence:

>1353_bases
ATGGCGCAATTTTTTAAAGCAAAACCAAATAGTTCCAAACAGTTGTCGGCTAAGTTATCGCTGAGCGTCAATCAGCTCGA
TCACTTAGGGGCAGGTATAGCACAACATCAAGGTAAAGTCGTGTTTATCCCCGGCGCACTGCCCGATGAAACCGTGACGG
TACAGCTCACCGAGCAAAAGAAAAATTATGCCCGCGCCAAGTTAATTAAAGTCGATACACCTAGCTCTGAGCGCGTGGAA
CCTGAGTGTCCACACTATCACACCTGCGGCGGCTGCGACTTACAGCATATGTCATTATCGGGCCAGCGTGAGCATAAAGA
AGCGGCGCTGCTGGATATTATGGCTAAGTTTGCCGGCGCCGAAGGCGGCGCTTTATCGCCCGCATTAACGGGTGAAGGGT
GGCATTATCGTCGCCGCGCCCGTCTTGCGACCTTATTTGATAAAAATACCAAGCATTTAAGCTTAGGTTTTCGTGCTGCC
AGTAGCAGTAATGTAGTGCCTATCAGCCAGTGTCAGGTGTTAGCTAAGCCGTTATCTGACTTGATCGTCCCCTTTGCTAA
GTTGCTGAATCAATTGTCGGCGAAAGCCAGCTTAGGCCATCTTGAGTTGATTGCCGCCGATAATGGTCACTTCGCCGTGC
TACGTATCACTAAGGCGCTAAACGATAAAGATCTCGCTAAGCTGTCAGCCTTTGCCGAGCAGCATCAAATTCACATTTGT
CTGCAGGACAATGAAGGCCAATTCCAAGGCGTTGGCGCTGAGTTAGTGTTACCGGTTTATCAGCTGTTAGATGACAAAGC
TGAGTCGGATGCGGTGAGTTTAAGCTTCACCCCAGGTAACTTTGTGCAAGTGAACGGACAGATTAACAAGGCCATGGTCG
CTCAAGCGATGGATTGGTTAGCGCCAGCGCCTGATGAGCGCATTTTGGATTTGTTCTGCGGCATGGGGAATTTTAGTTTG
CCCTTAGCGAAAATGGGCGCTGATGTGATTGGCGTCGAAGGGGTTGCCGAGATGGTAACTCAGGCACGGGTGAACGCGAA
AGCTAACAATCTTGATAAACTGACCTTTTATCACGGCGACTTGAGTGCGGATTTATCCCTTGAGCCTTGGATGGGCAAAA
TTGATAAATTACTGCTCGATCCCGCCCGAGCGGGTGCTTTTGAAAGCTTGCAATGGCTGAAGAAAATGAAGCCGCGCAAA
GTCGTGTATGTTTCTTGTAATCCTGCGAGCTTGGCCCGCGATAGCGCTGTACTGTTAGAGCGCGGTTACCGATTGCAGCA
ACTGGGACTTATCGACATGTTCCCACAGACACACCATATCGAAGCCATGGCACTGTTTGAATTAACCAAGTAA

Upstream 100 bases:

>100_bases
AGCAGCGGCGGTGTTTCCTGTGACAGAGACTAAGATAATTTTATAAGACTGCCAGAAAGAGGGCGGTCTTTGCCAGTATT
TTATTGATTTAGAGGCCGAA

Downstream 100 bases:

>100_bases
TTTGCGTTGGCAAAGTAAGCAAAGCGTTTGAATAACGTAAAAAATGTGATTATTACGTCGGTTGGGTTTGACAAGTTCGC
GCCAATGCTGAAGATAGGAG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQLTEQKKNYARAKLIKVDTPSSERVE
PECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGAEGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAA
SSSNVVPISQCQVLAKPLSDLIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWLAPAPDERILDLFCGMGNFSL
PLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFYHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRK
VVYVSCNPASLARDSAVLLERGYRLQQLGLIDMFPQTHHIEAMALFELTK

Sequences:

>Translated_450_residues
MAQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQLTEQKKNYARAKLIKVDTPSSERVE
PECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGAEGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAA
SSSNVVPISQCQVLAKPLSDLIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWLAPAPDERILDLFCGMGNFSL
PLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFYHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRK
VVYVSCNPASLARDSAVLLERGYRLQQLGLIDMFPQTHHIEAMALFELTK
>Mature_449_residues
AQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQLTEQKKNYARAKLIKVDTPSSERVEP
ECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGAEGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAAS
SSNVVPISQCQVLAKPLSDLIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHICL
QDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWLAPAPDERILDLFCGMGNFSLP
LAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFYHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKV
VYVSCNPASLARDSAVLLERGYRLQQLGLIDMFPQTHHIEAMALFELTK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=215, Percent_Identity=32.5581395348837, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI51173878, Length=215, Percent_Identity=32.5581395348837, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789148, Length=452, Percent_Identity=43.3628318584071, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787083, Length=177, Percent_Identity=32.7683615819209, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=27.8688524590164, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24666579, Length=198, Percent_Identity=32.3232323232323, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49107; Mature: 48976

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQLTEQK
CCCCCCCCCCCCCEEEEEEEEEHHHHHHHCCCHHHHCCCEEEECCCCCCCEEEEEEECCC
KNYARAKLIKVDTPSSERVEPECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
EGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAASSSNVVPISQCQVLAKPLSD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHH
LIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEE
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWL
EECCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
APAPDERILDLFCGMGNFSLPLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFYHGD
CCCCCHHHHHHHHCCCCCCCCHHHHCCCEECHHHHHHHHHHHHHCCCCCCCCEEEEEECC
LSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKVVYVSCNPASLARDSAVLLE
CCCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHH
RGYRLQQLGLIDMFPQTHHIEAMALFELTK
CCCCEECCCHHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
AQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQLTEQK
CCCCCCCCCCCCEEEEEEEEEHHHHHHHCCCHHHHCCCEEEECCCCCCCEEEEEEECCC
KNYARAKLIKVDTPSSERVEPECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
EGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAASSSNVVPISQCQVLAKPLSD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHH
LIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEE
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWL
EECCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
APAPDERILDLFCGMGNFSLPLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFYHGD
CCCCCHHHHHHHHCCCCCCCCHHHHCCCEECHHHHHHHHHHHHHCCCCCCCCEEEEEECC
LSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKVVYVSCNPASLARDSAVLLE
CCCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHH
RGYRLQQLGLIDMFPQTHHIEAMALFELTK
CCCCEECCCHHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA