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The map label for this gene is rlmN

Identifier: 160876257

GI number: 160876257

Start: 3725632

End: 3726753

Strand: Reverse

Name: rlmN

Synonym: Sbal195_3151

Alternate gene names: 160876257

Gene position: 3726753-3725632 (Counterclockwise)

Preceding gene: 160876260

Following gene: 160876256

Centisome position: 69.69

GC content: 46.61

Gene sequence:

>1122_bases
ATGAGTGAAAAGAAGATCAATTTATTAGACCTTGATCGTAAGGCGATGCGCGCATTATTTGCGGACATGGGGGAGAAACC
CTTTCGTGCCGATCAGCTCATGAAATGGCTATATCATTTCGGCGTCAGTGACTTCGAAGAGATGACCAACATCAACAAAG
TGTTACGTCAAAAATTGGCGGCACGCTGCGAGATCGTTGCGCCGGAAATTTCGAGCTTCCAAAAATCGACCGATGGGACG
ATTAAATTCGCGATCAACGTTGGTCAAGGCCAAGAAGTTGAAACGGTTTATATTCCTGAGGATGACCGCGCGACCTTGTG
TGTCTCATCCCAAGTGGGTTGTGCCCTCGAATGTACTTTCTGCTCCACGGGTCAGCAAGGGTTTAACCGTAACTTAACGG
TTTCTGAAATCGTCGGCCAAATTTGGCGTGTATCGCACTTTTTAGGTTTTGCTAAAGACACGGGCGAGCGTCCTATCACT
AACGTCGTGATGATGGGTATGGGTGAGCCATTGCTCAACTTAGCCAATGTGATCCCTGCGATGGACATCATGCTCGATGA
TTTTGGTTTTAGCTTGTCAAAACGCCGCGTGACCTTATCAACATCAGGCGTAGTGCCTGCGTTAGATAAACTGGGCGATG
CCATTGATGTGGCACTTGCCGTCAGTATCCATGCGCCAAATGATGAGCTGCGTGACATTCTGGTCCCAATCAATAAAAAA
TATCCGCTGGATGAGTTTCTAGCGGGTATTCGTCGTTATATCGCCAAGTCCAATGCAAACCGTGGCCGTGTGACGGTTGA
ATACGTGATGCTAGATCATATCAACGACAGCACAGATCAAGCCCATGAGTTAGCTAAGTTGATGAAAGATACACCTTGTA
AGGTAAATCTGATCCCATTTAACCCGTATCCTGGCTCGCCTTACGGCCGTTCGTCTAACTCACGTATCGACCGTTTTTCT
AAGGTGCTAATGGAGTACGGTTTTACAGTGATAGTGCGTAAAACCCGTGGCGATGATATTGATGCCGCCTGTGGTCAGTT
AGCTGGTGATATTCGCGATAGAACGAAACGTTTAGCAAAAAAACGCATGCAAGAAAACCAGATTTCAGTCACAATGAATT
AA

Upstream 100 bases:

>100_bases
TTCTAGCATAGGTTAATTGAGTTACTGGCTAAAATTTATTTGCGTCTGTATAATTCGCCGCCCTGTTACAGGATCCGGTT
TTGGTGTTGAGGTTAGCTAT

Downstream 100 bases:

>100_bases
CTCTTTGTTTGCACAGATTTATAGGCTTGCCCATGAAGCATGGATTGCCAAGGTTTTCGTTGATTTTAACCCTGTCATTA
TTGATGACAGGGTGCGTCAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGT
IKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPIT
NVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
YPLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Sequences:

>Translated_373_residues
MSEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGT
IKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPIT
NVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
YPLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN
>Mature_372_residues
SEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSFQKSTDGTI
KFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPITN
VVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKKY
PLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFSK
VLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=75.6830601092896, Blast_Score=595, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SHEB2 (B8E9S4)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357301.1
- ProteinModelPortal:   B8E9S4
- GeneID:   7088795
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1370
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK11194
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41684; Mature: 41552

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLA
CCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ARCEIVAPEISSFQKSTDGTIKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPITNVVMMGMGEPLLNLANVIPA
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCEEEEEEEEEECCCHHHHHHHCCCCCC
YPLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQENQISVTMN
HHHHCCCEEEEEC
>Mature Secondary Structure 
SEKKINLLDLDRKAMRALFADMGEKPFRADQLMKWLYHFGVSDFEEMTNINKVLRQKLA
CCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ARCEIVAPEISSFQKSTDGTIKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTGQQGFNRNLTVSEIVGQIWRVSHFLGFAKDTGERPITNVVMMGMGEPLLNLANVIPA
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDAIDVALAVSIHAPNDELRDILVPINKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCEEEEEEEEEECCCHHHHHHHCCCCCC
YPLDEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQENQISVTMN
HHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA