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The map label for this gene is mltF [H]

Identifier: 160876245

GI number: 160876245

Start: 3710440

End: 3711870

Strand: Direct

Name: mltF [H]

Synonym: Sbal195_3139

Alternate gene names: 160876245

Gene position: 3710440-3711870 (Clockwise)

Preceding gene: 160876240

Following gene: 160876249

Centisome position: 69.39

GC content: 49.62

Gene sequence:

>1431_bases
ATGACTCGATTCCTGTTCGCTCTTATCTTAGGTTTCTTACTGACTGCCTGTCAGCAGGTGACCGTGGACGAAACAGAATT
CGTGCCGAAAAAATTAACTGAGTTAAGAGTCGGCACACTCTACGGCCCACAGATTTATATGACATCTGGCCAAGGTGATA
GCGGTTTCGATTACGATATGGCGGTATTGTTCGCCGAATACTTAGATGTGCCCTTAAAAATGGTGCCCTACACTAACCGT
ACCGAGCTGTACGAAGCCCTTAAAAAGAATGAAATCGACCTCATTGCCGCGGGCATGACAGAAACGCCCGCAAGGCGTGA
ACAGTTCCGCCTAGGACCGCCGCTGTACCGCGTTAATCAAGTATTGGTGTATCGCGAAGGCATGCCAGCGCCCAAAGATA
TCAGCGACTTAAAGGGTAAGATCACTGTCATCGCCGACTCTTCCTTCGTCGAGACATTAACTCAATTGCAAAAGCATTAT
CCAACGCTGGTGTGGGATCAAATTACCGACAAAGACAGCGAAGAATTGCTGGCTATGATCGCCAACAAAGAAATTGACTA
CACCATAGCAGACTCCAGCAGCGTACAAATCAACCGCCGCTACTTACCCGATCTGCGCTCTGGCCCCGTACTTGAAGAAA
AGCTCGATGTAGTCTGGCTGTTGCCGCCGACCCGCAGTGATGAACTCATGAGTCAGTTACTGGCCTTTTGGCACCAAGAA
AAGCTGGCTGGCACGCTCGATCATCTCAACGAGAAATACTTTGGCCATGTGAAGCGCTTCGATTATGTCGATACTCGGGC
ATTTATCCGCGCTATTGAAACCGTTTTGCCCCGCTATCGTCAGTTATTTGAAACCCATGCGGGCAATTTAGATTGGCGTA
AGCTTGCGGCGACTAGCTATCAAGAGTCCCATTGGAATCCCCATGCTCGCTCGGCAACGGGGGTACGTGGCATGATGATG
TTAACTCAGCCTACGGCAAAAGAAATAGGCATCACCAACCGTTTGGATGCGGAAGAAAGCATCCGCGGCGGCGCAGCCTA
TTTGAACGATATGATCAATCGTTTGCCAGAATCCATTCCCGAGAGTCAACGTATGTGGTTTGCACTGGCGTCCTACAACA
TAGGATATGCCCATGTTGAAGATGCGCGAAAGCTGGCTGAATCGATGGAATTAAATCCCAATGCGTGGCGAGATTTAAAG
AAAGTGCTGCCACTGCTGCAAAAGCGTAAGTACTATCAAAAAACCCGCTACGGCTATGCCCGTGGCAGTGAAGCCGTCCA
TTACGTCGACAGTATTCGCCGCTACTACGACACATTAGTCTGGGTGGATAATCAGTCGAAACAGCAAAACCCCGAGGAAG
AACCGAGCGATCTTGCGTCGGAAGAACCCGCCATTCCCGCTGGAACATTAAGCCCAGAACAACCTAAGTAA

Upstream 100 bases:

>100_bases
AAACGGATATAATCCCACCAATGTCGGCCAGTTCACCACGCATGCCCCACTGTTAACTCGCTATCTATAATATTGATTTT
ATTTACTATAAGTACATTTA

Downstream 100 bases:

>100_bases
TTTGAATCGCCGGAGCCAAGCGTTAGAGCTAGGTTTCTAGGCACTGAGTTTAGAACCAGCTTCAAGACCTAAGCTCAGTG
ATCTCAGTGAGCTTAGGTTT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTRFLFALILGFLLTACQQVTVDETEFVPKKLTELRVGTLYGPQIYMTSGQGDSGFDYDMAVLFAEYLDVPLKMVPYTNR
TELYEALKKNEIDLIAAGMTETPARREQFRLGPPLYRVNQVLVYREGMPAPKDISDLKGKITVIADSSFVETLTQLQKHY
PTLVWDQITDKDSEELLAMIANKEIDYTIADSSSVQINRRYLPDLRSGPVLEEKLDVVWLLPPTRSDELMSQLLAFWHQE
KLAGTLDHLNEKYFGHVKRFDYVDTRAFIRAIETVLPRYRQLFETHAGNLDWRKLAATSYQESHWNPHARSATGVRGMMM
LTQPTAKEIGITNRLDAEESIRGGAAYLNDMINRLPESIPESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLK
KVLPLLQKRKYYQKTRYGYARGSEAVHYVDSIRRYYDTLVWVDNQSKQQNPEEEPSDLASEEPAIPAGTLSPEQPK

Sequences:

>Translated_476_residues
MTRFLFALILGFLLTACQQVTVDETEFVPKKLTELRVGTLYGPQIYMTSGQGDSGFDYDMAVLFAEYLDVPLKMVPYTNR
TELYEALKKNEIDLIAAGMTETPARREQFRLGPPLYRVNQVLVYREGMPAPKDISDLKGKITVIADSSFVETLTQLQKHY
PTLVWDQITDKDSEELLAMIANKEIDYTIADSSSVQINRRYLPDLRSGPVLEEKLDVVWLLPPTRSDELMSQLLAFWHQE
KLAGTLDHLNEKYFGHVKRFDYVDTRAFIRAIETVLPRYRQLFETHAGNLDWRKLAATSYQESHWNPHARSATGVRGMMM
LTQPTAKEIGITNRLDAEESIRGGAAYLNDMINRLPESIPESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLK
KVLPLLQKRKYYQKTRYGYARGSEAVHYVDSIRRYYDTLVWVDNQSKQQNPEEEPSDLASEEPAIPAGTLSPEQPK
>Mature_475_residues
TRFLFALILGFLLTACQQVTVDETEFVPKKLTELRVGTLYGPQIYMTSGQGDSGFDYDMAVLFAEYLDVPLKMVPYTNRT
ELYEALKKNEIDLIAAGMTETPARREQFRLGPPLYRVNQVLVYREGMPAPKDISDLKGKITVIADSSFVETLTQLQKHYP
TLVWDQITDKDSEELLAMIANKEIDYTIADSSSVQINRRYLPDLRSGPVLEEKLDVVWLLPPTRSDELMSQLLAFWHQEK
LAGTLDHLNEKYFGHVKRFDYVDTRAFIRAIETVLPRYRQLFETHAGNLDWRKLAATSYQESHWNPHARSATGVRGMMML
TQPTAKEIGITNRLDAEESIRGGAAYLNDMINRLPESIPESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLKK
VLPLLQKRKYYQKTRYGYARGSEAVHYVDSIRRYYDTLVWVDNQSKQQNPEEEPSDLASEEPAIPAGTLSPEQPK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=443, Percent_Identity=42.8893905191874, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 54653; Mature: 54522

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFLFALILGFLLTACQQVTVDETEFVPKKLTELRVGTLYGPQIYMTSGQGDSGFDYDM
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEECCEEEEECCCCCCCCCHHH
AVLFAEYLDVPLKMVPYTNRTELYEALKKNEIDLIAAGMTETPARREQFRLGPPLYRVNQ
HHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCCHHHHCE
VLVYREGMPAPKDISDLKGKITVIADSSFVETLTQLQKHYPTLVWDQITDKDSEELLAMI
EEEEECCCCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHHH
ANKEIDYTIADSSSVQINRRYLPDLRSGPVLEEKLDVVWLLPPTRSDELMSQLLAFWHQE
HCCCCCEEEECCCCEEECHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
KLAGTLDHLNEKYFGHVKRFDYVDTRAFIRAIETVLPRYRQLFETHAGNLDWRKLAATSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCH
QESHWNPHARSATGVRGMMMLTQPTAKEIGITNRLDAEESIRGGAAYLNDMINRLPESIP
HHHCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCC
ESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLKKVLPLLQKRKYYQKTRYGYA
CHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGSEAVHYVDSIRRYYDTLVWVDNQSKQQNPEEEPSDLASEEPAIPAGTLSPEQPK
CCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRFLFALILGFLLTACQQVTVDETEFVPKKLTELRVGTLYGPQIYMTSGQGDSGFDYDM
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEECCEEEEECCCCCCCCCHHH
AVLFAEYLDVPLKMVPYTNRTELYEALKKNEIDLIAAGMTETPARREQFRLGPPLYRVNQ
HHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCCHHHHCE
VLVYREGMPAPKDISDLKGKITVIADSSFVETLTQLQKHYPTLVWDQITDKDSEELLAMI
EEEEECCCCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHHH
ANKEIDYTIADSSSVQINRRYLPDLRSGPVLEEKLDVVWLLPPTRSDELMSQLLAFWHQE
HCCCCCEEEECCCCEEECHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
KLAGTLDHLNEKYFGHVKRFDYVDTRAFIRAIETVLPRYRQLFETHAGNLDWRKLAATSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCH
QESHWNPHARSATGVRGMMMLTQPTAKEIGITNRLDAEESIRGGAAYLNDMINRLPESIP
HHHCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCC
ESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLKKVLPLLQKRKYYQKTRYGYA
CHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGSEAVHYVDSIRRYYDTLVWVDNQSKQQNPEEEPSDLASEEPAIPAGTLSPEQPK
CCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]