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The map label for this gene is hisF

Identifier: 160875654

GI number: 160875654

Start: 3005397

End: 3006170

Strand: Direct

Name: hisF

Synonym: Sbal195_2542

Alternate gene names: 160875654

Gene position: 3005397-3006170 (Clockwise)

Preceding gene: 160875653

Following gene: 160875655

Centisome position: 56.2

GC content: 51.29

Gene sequence:

>774_bases
ATGCTGGCCAAACGACTAGTCCCCTGTTTAGATGTGAAAGACGGTAAAGTAGTGAAAGGCGTGCAGTTCCGTAACCACGA
AATTGTTGGTGATATCGTGCCCTTAGCCGCGCGTTATGCCGAAGAAGGCGCCGATGAGCTGGTGTTTTATGACATTACCG
CCAGCGCCCATGAACGTGTGGTCGATAAATCTTGGGTGAGCCGCGTGGCGGAGCAGATTGATATTCCCTTCTGCGTGGCA
GGTGGCATTAAAACCATTAGCCAAGCCCGTGAATTATTAGCCTTTGGGGCGGATAAGATTTCGATTAACTCGCCCGCCTT
AACCGATCCAAGTTTAATCTCACGACTGCAGGACGAGTTCGGCCGCCAGTGTATCGTGATTGGCATCGACTCCTTTTTCG
ATGCCACCAGCAACAGCTATAAGGTAAAGCAATTTACCGGCGATGAAGCGGCAACTAAGGATACGCAGTGGTTTACCCAA
GACTGGGTTGAAGAAGTGCAAAAACGAGGTTGTGGTGAAATCGTGCTCAATGTGATGAACCAAGACGGCGTGCGCGGCGG
CTACGATATCAAGCAGTTAAGTCTTGTTCGCGCCATCTGCGATGTGCCTTTAATTGCCTCGGGCGGCGCTGGGACTATGG
CGCATTTTCGCGATGTGTTTATTGAAGCCAAGGTCGATGCGGCGCTGGCAGCGAGCGTATTCCACAAGGCGATTATCAAC
ATTGGTGAGTTAAAAGCCTATTTAGCGGCCGAAGGCATTGCGATTAGGCGCTAG

Upstream 100 bases:

>100_bases
CCCTTGATGATGTTGCCGCCGTGCGTGACAGCGGCGCTGCGGGCATCATCATAGGCAAAGCCTTGCTTATCAATCAGTTT
AACGTGGTGGAGGCAATCCA

Downstream 100 bases:

>100_bases
CTATTGCAATTAGCCGCTCGCGGTTAATCGTGAATAGCGAGCTAATGGCGCCTACAGCTACAGTAAAAGCATGAGTAAGA
GCTAGCAACCACAATACGTC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERVVDKSWVSRVAEQIDIPFCVA
GGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEFGRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
IGELKAYLAAEGIAIRR

Sequences:

>Translated_257_residues
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERVVDKSWVSRVAEQIDIPFCVA
GGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEFGRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
IGELKAYLAAEGIAIRR
>Mature_257_residues
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERVVDKSWVSRVAEQIDIPFCVA
GGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEFGRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
IGELKAYLAAEGIAIRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=74.609375, Blast_Score=403, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=28.0254777070064, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SHEB2 (B8E999)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357855.1
- ProteinModelPortal:   B8E999
- SMR:   B8E999
- GeneID:   7090094
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1927
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28048; Mature: 28048

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
VDKSWVSRVAEQIDIPFCVAGGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQDWVEEVQKRGCGEIVLNVMN
CCEEEEEEEHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKAYLAAEGIAIRR
HHHHHHHHHHCCEECCC
>Mature Secondary Structure
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
VDKSWVSRVAEQIDIPFCVAGGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQDWVEEVQKRGCGEIVLNVMN
CCEEEEEEEHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKAYLAAEGIAIRR
HHHHHHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA