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The map label for this gene is bcr [H]

Identifier: 160875600

GI number: 160875600

Start: 2941977

End: 2943200

Strand: Direct

Name: bcr [H]

Synonym: Sbal195_2488

Alternate gene names: 160875600

Gene position: 2941977-2943200 (Clockwise)

Preceding gene: 160875598

Following gene: 160875601

Centisome position: 55.02

GC content: 49.02

Gene sequence:

>1224_bases
GTGGACCCACAATCACAACCTTCTGCCCATAACGGCAGACGTATACCGAATTTAGCGCTGCTTATTCCCATGCTTGCGGC
GATTGTCGCGATCACGCCATTGGCCATCGACATGTACTTACCTGCGATGTCGACATTGGCCCTCAGTTTTCATACCGATG
TCACGACAGTGCAGCAGTCCCTGAGTATCTACTTAGCGGGTTACGCGCTGGGCATGTTATGTTTCGGCCCCCTAGCCGAC
CGCATTGGTCGTCGCCCTATGGTGATATTGGGACTGAGTGGATTTATGCTCACCAGCTTACTGATAGGGTTTGCGGGTCA
AGTCGAAGTCTTTCTTGGACTACGTTTTTTGCAGGCTTTTATCGGCGCGGCTGCGACTGTGGTGGTACCGGGATACATCA
AAGAAGTCTATGGTGATAATACCGCCAAGGGTATGTCATACGTGAGTTTAATCATGATGTTAGCGCCATTACTCGCACCT
AGCATTGGCAGCGTGATCCTCGAACTCGGTGATTGGCATTTAATCTTCTTCATTCAATCCGCCTATGCCGCTACCCTGTT
ATTACTGGTGCTGTTTGCGCTGAAAATGCCCAGCGATAAAGATATGGATACCCGCAGCCAAAAATCGTTTCTTGGCGCCT
ATGCGACCGTATTTTCCCGCCCTGGCGTTAAGCTAAACATTGCCAGCGGCGTGTTAACGTCTTTTTCATTTTTCTGTTAT
CTCACCGCATCGCCCTTCGTTTACATGGAAGTCTTTGCCTTAGATAAATCCTATTTTGCCATCTTGTTTAGCACTAACGT
CGGCGCTTTGATGTTGGCCAATATCATTAATACGCGCATCGTGGGCCGTTATGGTTCGCTACGTATGTTGCATGTGTCGA
CCTTTTTCGGGGCGATTGCTGGTGTCGGTTTATTAACCGTAAACCTGCTGGATATGAGCTATCACTTTACCGTGATCATG
CTTATTCCTTTGATGGGTAGCCTAGGGGTGATGTCAGTCAATGCCGATGCGATAGTACTGATGAAGTTCAAGCAAGAAAC
GGGAACCGCAACCGCAGTTATTGGCACCTTAAGATTTGGCTGTGGCGCACTGGCGGGCCCGTTATTGGCGGTGTTTTACG
ATGGCACAGCCGTGCCCTTCTCTGCACTCATGTTAGGTTCTATTGTGTTAGTCGGTATTTGCCAATTCAACCGTCCAAAG
CATCAAATACCGCCGACGGTTTAA

Upstream 100 bases:

>100_bases
CAAAATGGCGGATTAATTGTGAATTGTTCTAGTACAATTTAAACCACACCGCATAAACCTGTGAAATACGCTTTCAATTC
TTTCGCCACCCGAGGTAACA

Downstream 100 bases:

>100_bases
GGATCTATTTTTAAGGGGCTATTTTTAAAAGCCATGGATACCAATGCCACATCGGCAACACATGTTTGATCGCGTGAAAA
CAGCGCATTGGCTTTCACAG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MDPQSQPSAHNGRRIPNLALLIPMLAAIVAITPLAIDMYLPAMSTLALSFHTDVTTVQQSLSIYLAGYALGMLCFGPLAD
RIGRRPMVILGLSGFMLTSLLIGFAGQVEVFLGLRFLQAFIGAAATVVVPGYIKEVYGDNTAKGMSYVSLIMMLAPLLAP
SIGSVILELGDWHLIFFIQSAYAATLLLLVLFALKMPSDKDMDTRSQKSFLGAYATVFSRPGVKLNIASGVLTSFSFFCY
LTASPFVYMEVFALDKSYFAILFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGAIAGVGLLTVNLLDMSYHFTVIM
LIPLMGSLGVMSVNADAIVLMKFKQETGTATAVIGTLRFGCGALAGPLLAVFYDGTAVPFSALMLGSIVLVGICQFNRPK
HQIPPTV

Sequences:

>Translated_407_residues
MDPQSQPSAHNGRRIPNLALLIPMLAAIVAITPLAIDMYLPAMSTLALSFHTDVTTVQQSLSIYLAGYALGMLCFGPLAD
RIGRRPMVILGLSGFMLTSLLIGFAGQVEVFLGLRFLQAFIGAAATVVVPGYIKEVYGDNTAKGMSYVSLIMMLAPLLAP
SIGSVILELGDWHLIFFIQSAYAATLLLLVLFALKMPSDKDMDTRSQKSFLGAYATVFSRPGVKLNIASGVLTSFSFFCY
LTASPFVYMEVFALDKSYFAILFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGAIAGVGLLTVNLLDMSYHFTVIM
LIPLMGSLGVMSVNADAIVLMKFKQETGTATAVIGTLRFGCGALAGPLLAVFYDGTAVPFSALMLGSIVLVGICQFNRPK
HQIPPTV
>Mature_407_residues
MDPQSQPSAHNGRRIPNLALLIPMLAAIVAITPLAIDMYLPAMSTLALSFHTDVTTVQQSLSIYLAGYALGMLCFGPLAD
RIGRRPMVILGLSGFMLTSLLIGFAGQVEVFLGLRFLQAFIGAAATVVVPGYIKEVYGDNTAKGMSYVSLIMMLAPLLAP
SIGSVILELGDWHLIFFIQSAYAATLLLLVLFALKMPSDKDMDTRSQKSFLGAYATVFSRPGVKLNIASGVLTSFSFFCY
LTASPFVYMEVFALDKSYFAILFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGAIAGVGLLTVNLLDMSYHFTVIM
LIPLMGSLGVMSVNADAIVLMKFKQETGTATAVIGTLRFGCGALAGPLLAVFYDGTAVPFSALMLGSIVLVGICQFNRPK
HQIPPTV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=392, Percent_Identity=31.1224489795918, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI48994889, Length=220, Percent_Identity=33.1818181818182, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790146, Length=251, Percent_Identity=28.6852589641434, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787065, Length=283, Percent_Identity=27.208480565371, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790794, Length=253, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082312, Length=197, Percent_Identity=26.9035532994924, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43651; Mature: 43651

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPQSQPSAHNGRRIPNLALLIPMLAAIVAITPLAIDMYLPAMSTLALSFHTDVTTVQQS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LSIYLAGYALGMLCFGPLADRIGRRPMVILGLSGFMLTSLLIGFAGQVEVFLGLRFLQAF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHH
IGAAATVVVPGYIKEVYGDNTAKGMSYVSLIMMLAPLLAPSIGSVILELGDWHLIFFIQS
HCCHHEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEHHHH
AYAATLLLLVLFALKMPSDKDMDTRSQKSFLGAYATVFSRPGVKLNIASGVLTSFSFFCY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
LTASPFVYMEVFALDKSYFAILFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGAIA
HHCCCCEEHHHEEECCCEEEEEEECCCHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHH
GVGLLTVNLLDMSYHFTVIMLIPLMGSLGVMSVNADAIVLMKFKQETGTATAVIGTLRFG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCCCHHHHHHHHHHH
CGALAGPLLAVFYDGTAVPFSALMLGSIVLVGICQFNRPKHQIPPTV
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDPQSQPSAHNGRRIPNLALLIPMLAAIVAITPLAIDMYLPAMSTLALSFHTDVTTVQQS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LSIYLAGYALGMLCFGPLADRIGRRPMVILGLSGFMLTSLLIGFAGQVEVFLGLRFLQAF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHH
IGAAATVVVPGYIKEVYGDNTAKGMSYVSLIMMLAPLLAPSIGSVILELGDWHLIFFIQS
HCCHHEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEHHHH
AYAATLLLLVLFALKMPSDKDMDTRSQKSFLGAYATVFSRPGVKLNIASGVLTSFSFFCY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
LTASPFVYMEVFALDKSYFAILFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGAIA
HHCCCCEEHHHEEECCCEEEEEEECCCHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHH
GVGLLTVNLLDMSYHFTVIMLIPLMGSLGVMSVNADAIVLMKFKQETGTATAVIGTLRFG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCCCHHHHHHHHHHH
CGALAGPLLAVFYDGTAVPFSALMLGSIVLVGICQFNRPKHQIPPTV
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]