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The map label for this gene is lcrF [H]

Identifier: 160875374

GI number: 160875374

Start: 2685885

End: 2686583

Strand: Direct

Name: lcrF [H]

Synonym: Sbal195_2262

Alternate gene names: 160875374

Gene position: 2685885-2686583 (Clockwise)

Preceding gene: 160875373

Following gene: 160875375

Centisome position: 50.23

GC content: 40.2

Gene sequence:

>699_bases
ATGAGTGCAATACAAATAATTTTGTTTCGACAGCCAGGGAAAATCCACTATCAGGGTGAAAACCTAGCGGTCGGCAGTAA
TCAGTTAGTTGTAACCACGGAGGAGGCAACAGCTTCTGCTTTGAGTCGTGACAATTGGATGAGCATCTTTTGTTATCCAG
GTGAGCTACACGCATTATTTCACGAAATTGCCGATCTGATTGGTCCGAGTGCTGAGATGAATCGATTTCAACCTGAATCA
ACAAAAATACTCCCTGTATTTGATGAGTTAAGCAATGTCATTGAACAGTTGAAAGGTAATCATAATTTATTGATTAAGTT
TATTTTTATGTACTGTCTTGGCATGGATAACCGTTACTTTTCCGGTTTAATGCGTTACTTCTTAGCCCATAACGACAAGA
TGCTGAGCTATCTTGAGGGCAATTTTATGCAACCTTGGCCAGTCACACGCTTTGCCGAAAATTTAGGCATTGAAGTACGC
CGACTCAATTTCTTGTTTTATAAAAACTATGGTGTATCGGCAAAGCAATGGTTGTTAGAACGGCGCTTAAGTTATGCCCG
TCAGCAACTGATAGTGACTGAGAAAAAAGTCGCCGATATTGCCTTAGATAGTGGTTTTAGTAATCACGCACATTTTACCG
AATCGTTTAAGAAACGTTATTTATGCAGCCCAACCGTACTGCGTGCAACCTTTGTATAA

Upstream 100 bases:

>100_bases
GCTGCATTTAAGCGAATTCAGATCTCAGGTTAACACCTGATGTCAACCTATCCGCTCGCGTTTCACAATGAATCATAGTT
TGCAGTAAGGGTTAATCCAT

Downstream 100 bases:

>100_bases
GGAGGTCCCATGGACTTAAGTGCCATAGTTAATCAATTGTCTCAGCTATCGACGCGCTCAGCTCAAGAGGTACAGGCCAA
GATGTCTGCAGGCGATTTGA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSAIQIILFRQPGKIHYQGENLAVGSNQLVVTTEEATASALSRDNWMSIFCYPGELHALFHEIADLIGPSAEMNRFQPES
TKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNRYFSGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAENLGIEVR
RLNFLFYKNYGVSAKQWLLERRLSYARQQLIVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRATFV

Sequences:

>Translated_232_residues
MSAIQIILFRQPGKIHYQGENLAVGSNQLVVTTEEATASALSRDNWMSIFCYPGELHALFHEIADLIGPSAEMNRFQPES
TKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNRYFSGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAENLGIEVR
RLNFLFYKNYGVSAKQWLLERRLSYARQQLIVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRATFV
>Mature_231_residues
SAIQIILFRQPGKIHYQGENLAVGSNQLVVTTEEATASALSRDNWMSIFCYPGELHALFHEIADLIGPSAEMNRFQPEST
KILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNRYFSGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAENLGIEVRR
LNFLFYKNYGVSAKQWLLERRLSYARQQLIVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRATFV

Specific function: Transcriptional activator of the thermally regulated virulent yopE gene. LcrF activity could be modulated by the interaction with an inducer molecule serving as a temperature messenger. The availability of the messenger would in turn be controlled by a te

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 26822; Mature: 26691

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIQIILFRQPGKIHYQGENLAVGSNQLVVTTEEATASALSRDNWMSIFCYPGELHALF
CCCEEEEEEECCCEEEECCCEEEECCCEEEEEECHHHHHHHCCCCCEEEEEECCHHHHHH
HEIADLIGPSAEMNRFQPESTKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNRYF
HHHHHHHCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
SGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAENLGIEVRRLNFLFYKNYGVSAKQWLLE
HHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHH
RRLSYARQQLIVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRATFV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHEECC
>Mature Secondary Structure 
SAIQIILFRQPGKIHYQGENLAVGSNQLVVTTEEATASALSRDNWMSIFCYPGELHALF
CCEEEEEEECCCEEEECCCEEEECCCEEEEEECHHHHHHHCCCCCEEEEEECCHHHHHH
HEIADLIGPSAEMNRFQPESTKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNRYF
HHHHHHHCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
SGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAENLGIEVRRLNFLFYKNYGVSAKQWLLE
HHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHH
RRLSYARQQLIVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRATFV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624422; 9746557; 9748454; 11586360 [H]