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The map label for this gene is pleD [H]

Identifier: 160875204

GI number: 160875204

Start: 2491396

End: 2492373

Strand: Reverse

Name: pleD [H]

Synonym: Sbal195_2091

Alternate gene names: 160875204

Gene position: 2492373-2491396 (Counterclockwise)

Preceding gene: 160875206

Following gene: 160875203

Centisome position: 46.61

GC content: 39.57

Gene sequence:

>978_bases
ATGGAACTAAAGGAACAGTATTCTATTCCTCGTATCCCAGCGATACTTTTTATGCTTGCCGCAATACTGACACTGTGTAC
CTTCGTTATTTACCGTTACATCCACCAAGACTACATCCTTACTTTTGTCTTTGGCATCGCCTTATTACCCTTATGCATTA
CATTAATCTTAGAGATAAGGCGTAAATCGAATTTATTCCAGAAGCAACTGACGCTACTCATAATCTGTGGGGCCATCATT
TTTAGCTGTTATGAAATAGGCTATAAAGGATTAATTTATATTTCTCCAACAGTATTTATCTTCTTTTTCCTTTTCAATCT
AAAATCAAGCCTAATATTGAGCACCCTCTATGCCTGTATCAGTTTAGTGAGTGCATTACAGGTAGAGTCAGTCGAGTTAG
TTCTGAGGTTCTCAGTCGCCACCTTTGATTGCATTATCTTTGCCGCTATTTTTGCGCATATCATTAATAAACAGAGACAA
GCTCTAGTCCATTTAGCCAATACGGATGAGCTCACAGGAGCACTCAACCGCAAACAACTCAAAACTAAGCTTGAGCAGAG
CGTCTATGAGTATCAGCAACAGCAAATTCCTGCTAGTTTACTCTTGATTGATATCGATTTTTTCAAAACGGTGAACGATA
ATTTTGGTCATATCATAGGCGACCAATTACTCAATAAATTCAGTCAACATATCCAAAAAAAGATCAGAAAAGACGACTTA
TTATTCCGTTTCGGCGGAGAAGAATTTCTGATCTTACTACCCAATACTCAATTTGAGTCAGCTGCCATTGTCGCCGAAGA
CCTTAGACGAAGTATTGAAGAACAACACTTTCTCGACCAACACACCCAAGTCACTTGCAGTATCGGAGTTGCTGATTTAC
GTCAAGCTGACACCTTAGACACTTGGCTTAAAACCTGCGATAACCTGCTGTATAGCGCTAAAAATGCAGGACGTAATTGT
GTTGTCGCTCGCGCGTAA

Upstream 100 bases:

>100_bases
TGAGTCGCTACAAGTTCTAGTAAAAACTGCCATTATATGCGCTGTAATAATGACTTGTGAGCTAAACTAGCCTCATCAAT
GCTTAATAACGGTAGTAAAT

Downstream 100 bases:

>100_bases
TCATCCGTCTGACTATAGCGACGAATGAGTGATGCAAAGAATCACTATCCAAGCCCACAAAAATCAAAATAAACTCGCCA
AATGCACAAGCCCTTATTTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIRRKSNLFQKQLTLLIICGAII
FSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACISLVSALQVESVELVLRFSVATFDCIIFAAIFAHIINKQRQ
ALVHLANTDELTGALNRKQLKTKLEQSVYEYQQQQIPASLLLIDIDFFKTVNDNFGHIIGDQLLNKFSQHIQKKIRKDDL
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHTQVTCSIGVADLRQADTLDTWLKTCDNLLYSAKNAGRNC
VVARA

Sequences:

>Translated_325_residues
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIRRKSNLFQKQLTLLIICGAII
FSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACISLVSALQVESVELVLRFSVATFDCIIFAAIFAHIINKQRQ
ALVHLANTDELTGALNRKQLKTKLEQSVYEYQQQQIPASLLLIDIDFFKTVNDNFGHIIGDQLLNKFSQHIQKKIRKDDL
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHTQVTCSIGVADLRQADTLDTWLKTCDNLLYSAKNAGRNC
VVARA
>Mature_325_residues
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIRRKSNLFQKQLTLLIICGAII
FSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACISLVSALQVESVELVLRFSVATFDCIIFAAIFAHIINKQRQ
ALVHLANTDELTGALNRKQLKTKLEQSVYEYQQQQIPASLLLIDIDFFKTVNDNFGHIIGDQLLNKFSQHIQKKIRKDDL
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHTQVTCSIGVADLRQADTLDTWLKTCDNLLYSAKNAGRNC
VVARA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=39.6551724137931, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787262, Length=205, Percent_Identity=36.0975609756098, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787802, Length=192, Percent_Identity=32.8125, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=34.9397590361446, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=33.5403726708075, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=36.25, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081974, Length=147, Percent_Identity=34.0136054421769, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=32.1637426900585, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=31.25, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=28.8461538461538, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787056, Length=236, Percent_Identity=26.271186440678, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.3170731707317, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 37151; Mature: 37151

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIR
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSNLFQKQLTLLIICGAIIFSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SLVSALQVESVELVLRFSVATFDCIIFAAIFAHIINKQRQALVHLANTDELTGALNRKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KTKLEQSVYEYQQQQIPASLLLIDIDFFKTVNDNFGHIIGDQLLNKFSQHIQKKIRKDDL
HHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCE
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHTQVTCSIGVADLRQADTLD
EEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHH
TWLKTCDNLLYSAKNAGRNCVVARA
HHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIR
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSNLFQKQLTLLIICGAIIFSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SLVSALQVESVELVLRFSVATFDCIIFAAIFAHIINKQRQALVHLANTDELTGALNRKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KTKLEQSVYEYQQQQIPASLLLIDIDFFKTVNDNFGHIIGDQLLNKFSQHIQKKIRKDDL
HHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCE
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHTQVTCSIGVADLRQADTLD
EEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHH
TWLKTCDNLLYSAKNAGRNCVVARA
HHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA