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The map label for this gene is grpE

Identifier: 160874497

GI number: 160874497

Start: 1653048

End: 1653668

Strand: Direct

Name: grpE

Synonym: Sbal195_1379

Alternate gene names: 160874497

Gene position: 1653048-1653668 (Clockwise)

Preceding gene: 160874489

Following gene: 160874503

Centisome position: 30.91

GC content: 46.86

Gene sequence:

>621_bases
ATGAGCAACGAATCGATTAAAGCAGAACAGGATCTGATCCAAGAGGGTGTAGAGTCTGAAGTATCTACCGCAGAAGCAAG
TCTGATCGATGAGCTTACCCAGGCCAATTTCCGTATTGAAGAGCTGGAACAGTTACTCGCTGAAGCTTTGGCGAAAGTTG
AAGAGCAGAAGGATTCAGTCATCCGCGCCGCAGCCGAAGTCGATAATATTCGTCGTCGTGCCGCAATGGATGTGGAAAAG
GCGAACAAGTTTGCCTTGGAAAAGTTCGCCAATGAACTGTTGCCAGTACTTGATAACATGGAGCGCGCATTGATGGGGAC
TAACCCAGAAGATGAAGCGACTAAATCGATTTATCAAGGTGTTGAGTTAACTCAAAAAAGCTTACTCACTGCGGTGGCTA
AATTTGGTGTTAAGCAAATTGATCCACAAGGTCAGTCATTTAACCCAGATCAACATCAAGCGATTGGCATGCAGCCAAGC
GCTGAGTTCCCTGCGAACACTGTGATGTTGGTGATGCAAAAGGGTTATGAGCTGAACAGTCGCTTGCTGCGTCCTGCTAT
GGTAATGGTGTCACAGGGTGGCCCTAACCAAGAAAGCGCAACGATAGATATCGAAGCTTAA

Upstream 100 bases:

>100_bases
ATTTATGCTAGATCTGGCCTTAGGCCTTGAAACGTCAAAATTGATCCCCATAATATGCCCAGAGTATTTCAGAAACAAAG
CATTTTTAGCTGGAGTGAAA

Downstream 100 bases:

>100_bases
GCTTTATTGGATCAGCCGCTGTTATAACAGACTGTCCCGTCCTAAGATAGGTTGACAGTTTCTAGGCCAGCTCCAGTAGC
TGGCCTTTTCTATTATGCAC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSNESIKAEQDLIQEGVESEVSTAEASLIDELTQANFRIEELEQLLAEALAKVEEQKDSVIRAAAEVDNIRRRAAMDVEK
ANKFALEKFANELLPVLDNMERALMGTNPEDEATKSIYQGVELTQKSLLTAVAKFGVKQIDPQGQSFNPDQHQAIGMQPS
AEFPANTVMLVMQKGYELNSRLLRPAMVMVSQGGPNQESATIDIEA

Sequences:

>Translated_206_residues
MSNESIKAEQDLIQEGVESEVSTAEASLIDELTQANFRIEELEQLLAEALAKVEEQKDSVIRAAAEVDNIRRRAAMDVEK
ANKFALEKFANELLPVLDNMERALMGTNPEDEATKSIYQGVELTQKSLLTAVAKFGVKQIDPQGQSFNPDQHQAIGMQPS
AEFPANTVMLVMQKGYELNSRLLRPAMVMVSQGGPNQESATIDIEA
>Mature_205_residues
SNESIKAEQDLIQEGVESEVSTAEASLIDELTQANFRIEELEQLLAEALAKVEEQKDSVIRAAAEVDNIRRRAAMDVEKA
NKFALEKFANELLPVLDNMERALMGTNPEDEATKSIYQGVELTQKSLLTAVAKFGVKQIDPQGQSFNPDQHQAIGMQPSA
EFPANTVMLVMQKGYELNSRLLRPAMVMVSQGGPNQESATIDIEA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=158, Percent_Identity=32.9113924050633, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI40255109, Length=143, Percent_Identity=32.1678321678322, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI1788967, Length=179, Percent_Identity=42.4581005586592, Blast_Score=135, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17552458, Length=159, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=171, Percent_Identity=34.5029239766082, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653432, Length=165, Percent_Identity=32.1212121212121, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_SHEB2 (B8EAU9)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002358914.1
- ProteinModelPortal:   B8EAU9
- SMR:   B8EAU9
- GeneID:   7087942
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_3005
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14150
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22680; Mature: 22549

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNESIKAEQDLIQEGVESEVSTAEASLIDELTQANFRIEELEQLLAEALAKVEEQKDSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IRAAAEVDNIRRRAAMDVEKANKFALEKFANELLPVLDNMERALMGTNPEDEATKSIYQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VELTQKSLLTAVAKFGVKQIDPQGQSFNPDQHQAIGMQPSAEFPANTVMLVMQKGYELNS
HHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCHHH
RLLRPAMVMVSQGGPNQESATIDIEA
HHHHHHHHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
SNESIKAEQDLIQEGVESEVSTAEASLIDELTQANFRIEELEQLLAEALAKVEEQKDSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IRAAAEVDNIRRRAAMDVEKANKFALEKFANELLPVLDNMERALMGTNPEDEATKSIYQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VELTQKSLLTAVAKFGVKQIDPQGQSFNPDQHQAIGMQPSAEFPANTVMLVMQKGYELNS
HHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCHHH
RLLRPAMVMVSQGGPNQESATIDIEA
HHHHHHHHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA