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The map label for this gene is ptsP [H]

Identifier: 160874381

GI number: 160874381

Start: 1501436

End: 1503670

Strand: Direct

Name: ptsP [H]

Synonym: Sbal195_1262

Alternate gene names: 160874381

Gene position: 1501436-1503670 (Clockwise)

Preceding gene: 160874380

Following gene: 160874382

Centisome position: 28.08

GC content: 49.49

Gene sequence:

>2235_bases
GTGTTAAATACGCTTAGGGATATTACTCAGGCTGTGGCATCTGCCCACAGTTTGGAAACCGCATTAGAAGTCTTAGTCTC
ATCGACTAAAGCGGCGATGGAAACCCAATGTTGCTCCATCTATATCCTAGAAGAGCAAGAACTGGTGTTATCTGCAACTG
ACGGTCTTGAAAAAAGCGCCGTTGGTCGGGTACGTATGCCACTGACCGAAGGTTTAGTCGGACTCGCAGCCCAGCGGGAA
GAGGCCGTTAACTTAGCGGATGCAAGGCTTCATCCCAGATTCAAACTTTTCCCTGAAGTCGCCGAAGAAGAGTACCGCGC
CTTTCTAGCCGTACCTATCATCTACCAGAAAGCGGTGGTTGGGGTGATAGTGGTACAGCAGGCGAGTGCGCGCCAATTCA
GTGAAGGTGAAGAAGCTTTCCTGATGACACTCGCCGCCCAGTTAGCGATGGCGGTGCGCGGCTTAAAACAAAAAGCCCAA
GTCAGTTCCCATCATCAACAAATTCTATTTCAAGGTACGTCGGCGTCAAACGGTATCGCCATTGCGTATGCCTTTGTGCT
CGGTGGAGAAATCCCCCTTGAGCAGCCCGACGTGCGCTCTGACGACATTACCTATGAGTCCAGCCGCTTAACGACGGCTA
TGGTGCGTTGTAAAGAAGCGATTGGTGCTTTGTCTCAGCGGTTTGATCGCGAGCAAGATGACGAAGTCGTCTCTATTTTC
AATGCATTACAGCTATTACTTGATGATGCCAGTTTAGGCGGAGAGTATGCCCGCGAGGTGCAATTAGGTTGGGCTGCTGA
ATCGGCGGTGAGCCGAGTGTCTTTGCGTTATATTCAGCAATTCTTGGCGATGGAAGATCCTTATCTTAAGGAGCGGGCAA
GCGATATCCGCGACTTAGGCCAAAAAGTATTGCGCCAGCTGATTGAGCCAGAGCGTTTAGAGCTTGAACCCGATAAACCT
GTCATTTTAATTACCCGCGAAGCCGATGCGACTATGCTGGCTGAGTTCCCAAGGCAGAAACTTGCGGGGATTGTCACTGA
GCTCGGCGGCGTAAACTCCCATGCCGCTATTTTAGCCCGCGCCTTAGGTGTGCCGGCCATTACCGGTGTAGAACAACTGT
TATCCGCCAATATTGATCAAAAACTCTTAGTGGTGAACGCCAGCCGTGGCCAGCTATTGGTGTCGCCATCACCGGCGATT
GTTGGTGAGTATCGCAGCTTAATTTCGGCGCAAAAAGCGCTGCAACGCCAGTATGCTCAAGAATTAGCACTGCCTTCTGT
TACCTTAGATGGCTCACGTATTCGTTTGTATTTAAATGCGGGCTTACTCAGCGGCGTGGCCTCTGAAATTGCCGAAGGCG
CCGACGGGATTGGACTGTATCGCACCGAAATTCCTTTTATGCTGCAACAACGTTTCCCGAGTGAATCTGAGCAAGTGAAA
GTGTATCAACAGGTCTTGTCGGCGGCGTCTGGTCGACCTGTGGTGATGCGCACTTTGGATGTTGGTGGTGATAAACCTTT
GCCGTATTTCCCAATTAAAGAAGACAATCCTTTCCTCGGTTGGCGCGGGATCCGTTTGTCGCTGGATCATCCTGAGCTAT
TTTTGGTGCAGTTACGGGCCATGTTGCAGGCAGGTGGCGATGGTAAGCAATTAAGTATTTTACTGCCCATGGTCAGTAAC
TTAGATGAAATCGATCAATCCTTAGAATATCTGCAGCAAGCCTATGTTGAACTTAAGAACGATGTAAACAGCAACATAGT
GATGCCACGCATCGGCATTATGCTCGAAGTGCCGGCTATGTTGTATCAATTGGATGATGTGGCAAAACGGGTGGATTTTG
TCTCCGTTGGCAGTAATGACTTAACCCAGTATTTGCTTGCGGTTGACCGTAACAATCCGCGGGTGAGCTCATTATTCGAC
AGTTATCATCCGGGAATACTGCGGGCTTTGCATCAGGCTCACTTAGATTGTCGATATCATAAGCTCGATATCAGTATTTG
TGGCGAGTTAGCGGGCGAGCCTATGGGCGCCATTTTGTTGGTGGCTATGGGATACGATCAATTGAGTATGAATCAGGGCA
GTTTAGCGCGGATCAATTATCTATTGCGCCGTGTCGCGCGGGCCGATTTGGATCAATTGCTCGCTAAAGCCTTGAGCCTA
TCAAACGGCTTCCAAGTGCGCGAGCTGGTTAAAGAATATTTGAACCAGCAAGGTCTCTCAACAATTCTTAATTAA

Upstream 100 bases:

>100_bases
AGTTTGCGGTAACGGCATTGTCATTCCAGACCCTAGAAATACCTAGGAAACGGGGAAGGCAAAAAACAACGGGCTGATTT
TCAAAAGAGGATTGAAAACA

Downstream 100 bases:

>100_bases
CTAATGTCGTCTTTTGCTTTAAGCTAAGGCCCTCAATTTATCACGCTGTAGGTACTCGTGTGGATAGTTTGTTGTCGGTG
TTTTTTATTTGCTTAGCTTT

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSIYILEEQELVLSATDGLEKSAVGRVRMPLTEGLVGLAAQRE
EAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVVGVIVVQQASARQFSEGEEAFLMTLAAQLAMAVRGLKQKAQ
VSSHHQQILFQGTSASNGIAIAYAFVLGGEIPLEQPDVRSDDITYESSRLTTAMVRCKEAIGALSQRFDREQDDEVVSIF
NALQLLLDDASLGGEYAREVQLGWAAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLGQKVLRQLIEPERLELEPDKP
VILITREADATMLAEFPRQKLAGIVTELGGVNSHAAILARALGVPAITGVEQLLSANIDQKLLVVNASRGQLLVSPSPAI
VGEYRSLISAQKALQRQYAQELALPSVTLDGSRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAGGDGKQLSILLPMVSN
LDEIDQSLEYLQQAYVELKNDVNSNIVMPRIGIMLEVPAMLYQLDDVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFD
SYHPGILRALHQAHLDCRYHKLDISICGELAGEPMGAILLVAMGYDQLSMNQGSLARINYLLRRVARADLDQLLAKALSL
SNGFQVRELVKEYLNQQGLSTILN

Sequences:

>Translated_744_residues
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSIYILEEQELVLSATDGLEKSAVGRVRMPLTEGLVGLAAQRE
EAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVVGVIVVQQASARQFSEGEEAFLMTLAAQLAMAVRGLKQKAQ
VSSHHQQILFQGTSASNGIAIAYAFVLGGEIPLEQPDVRSDDITYESSRLTTAMVRCKEAIGALSQRFDREQDDEVVSIF
NALQLLLDDASLGGEYAREVQLGWAAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLGQKVLRQLIEPERLELEPDKP
VILITREADATMLAEFPRQKLAGIVTELGGVNSHAAILARALGVPAITGVEQLLSANIDQKLLVVNASRGQLLVSPSPAI
VGEYRSLISAQKALQRQYAQELALPSVTLDGSRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAGGDGKQLSILLPMVSN
LDEIDQSLEYLQQAYVELKNDVNSNIVMPRIGIMLEVPAMLYQLDDVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFD
SYHPGILRALHQAHLDCRYHKLDISICGELAGEPMGAILLVAMGYDQLSMNQGSLARINYLLRRVARADLDQLLAKALSL
SNGFQVRELVKEYLNQQGLSTILN
>Mature_744_residues
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSIYILEEQELVLSATDGLEKSAVGRVRMPLTEGLVGLAAQRE
EAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVVGVIVVQQASARQFSEGEEAFLMTLAAQLAMAVRGLKQKAQ
VSSHHQQILFQGTSASNGIAIAYAFVLGGEIPLEQPDVRSDDITYESSRLTTAMVRCKEAIGALSQRFDREQDDEVVSIF
NALQLLLDDASLGGEYAREVQLGWAAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLGQKVLRQLIEPERLELEPDKP
VILITREADATMLAEFPRQKLAGIVTELGGVNSHAAILARALGVPAITGVEQLLSANIDQKLLVVNASRGQLLVSPSPAI
VGEYRSLISAQKALQRQYAQELALPSVTLDGSRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAGGDGKQLSILLPMVSN
LDEIDQSLEYLQQAYVELKNDVNSNIVMPRIGIMLEVPAMLYQLDDVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFD
SYHPGILRALHQAHLDCRYHKLDISICGELAGEPMGAILLVAMGYDQLSMNQGSLARINYLLRRVARADLDQLLAKALSL
SNGFQVRELVKEYLNQQGLSTILN

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=747, Percent_Identity=43.6412315930388, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1788756, Length=600, Percent_Identity=32.1666666666667, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1788726, Length=541, Percent_Identity=34.5656192236599, Blast_Score=275, Evalue=1e-74,
Organism=Escherichia coli, GI48994992, Length=512, Percent_Identity=33.0078125, Blast_Score=274, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 81848; Mature: 81848

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSIYILEEQELVLSATDGLEKSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCHHC
VGRVRMPLTEGLVGLAAQREEAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVV
CCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHH
GVIVVQQASARQFSEGEEAFLMTLAAQLAMAVRGLKQKAQVSSHHQQILFQGTSASNGIA
HHHEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCE
IAYAFVLGGEIPLEQPDVRSDDITYESSRLTTAMVRCKEAIGALSQRFDREQDDEVVSIF
EEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NALQLLLDDASLGGEYAREVQLGWAAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLG
HHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QKVLRQLIEPERLELEPDKPVILITREADATMLAEFPRQKLAGIVTELGGVNSHAAILAR
HHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALGVPAITGVEQLLSANIDQKLLVVNASRGQLLVSPSPAIVGEYRSLISAQKALQRQYAQ
HHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
ELALPSVTLDGSRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
HHCCCCEEECCCEEEEEEECHHHHHHHHHHHCCCCCCEEEHHHCHHHHHHHCCCCHHHHH
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRA
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MLQAGGDGKQLSILLPMVSNLDEIDQSLEYLQQAYVELKNDVNSNIVMPRIGIMLEVPAM
HHHCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHH
LYQLDDVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFDSYHPGILRALHQAHLDCRYH
HHHHHHHHHHHCEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEE
KLDISICGELAGEPMGAILLVAMGYDQLSMNQGSLARINYLLRRVARADLDQLLAKALSL
EEHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
SNGFQVRELVKEYLNQQGLSTILN
CCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSIYILEEQELVLSATDGLEKSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCHHC
VGRVRMPLTEGLVGLAAQREEAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVV
CCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHH
GVIVVQQASARQFSEGEEAFLMTLAAQLAMAVRGLKQKAQVSSHHQQILFQGTSASNGIA
HHHEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCE
IAYAFVLGGEIPLEQPDVRSDDITYESSRLTTAMVRCKEAIGALSQRFDREQDDEVVSIF
EEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NALQLLLDDASLGGEYAREVQLGWAAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLG
HHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QKVLRQLIEPERLELEPDKPVILITREADATMLAEFPRQKLAGIVTELGGVNSHAAILAR
HHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALGVPAITGVEQLLSANIDQKLLVVNASRGQLLVSPSPAIVGEYRSLISAQKALQRQYAQ
HHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
ELALPSVTLDGSRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
HHCCCCEEECCCEEEEEEECHHHHHHHHHHHCCCCCCEEEHHHCHHHHHHHCCCCHHHHH
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRA
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MLQAGGDGKQLSILLPMVSNLDEIDQSLEYLQQAYVELKNDVNSNIVMPRIGIMLEVPAM
HHHCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHH
LYQLDDVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFDSYHPGILRALHQAHLDCRYH
HHHHHHHHHHHCEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEE
KLDISICGELAGEPMGAILLVAMGYDQLSMNQGSLARINYLLRRVARADLDQLLAKALSL
EEHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
SNGFQVRELVKEYLNQQGLSTILN
CCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]