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The map label for this gene is macA [H]

Identifier: 160873954

GI number: 160873954

Start: 974389

End: 975639

Strand: Direct

Name: macA [H]

Synonym: Sbal195_0832

Alternate gene names: 160873954

Gene position: 974389-975639 (Clockwise)

Preceding gene: 160873953

Following gene: 160873955

Centisome position: 18.22

GC content: 50.68

Gene sequence:

>1251_bases
ATGGACACGACAATACCGATGAAAAAATCCTCAAAGCGTAAATTGCTGCTCGCGTTAAGCGGCTTAATCCTCCTGGGTGG
TGGTGCTTATTTTATGTGGCATAAGCCTGAAACGGCACCTGCCTATGTGACAGAAGCGGTGCGCCGTGGTGATATCGAAA
ACTCAGTGTTGGCCAATGGCATGTTGCAGGCCTCTAAACTGGTGAGTGTGGGCGCGCAGGTATCGGGGCAAATCTTAAGT
TTGCCGCTGGCACTGGGGGATGAAGTAAAGAAAGGCGATTTGATTGCTCAAATTGATAGCCTAGCGCAGCAGAATAATTT
GAAGGATGCCCTCGCATCACTCAAGAGTATCAATGCCCAGTTCCGCGCCAAACAGGCGCAGATCCGCCAAGCCAAGTTGG
AGTTTAGCCGCCAGCAGCAAATGCTTGCCGACAAGGCCAGTTCCCGCGCAGATTATGAAGTGGCCGATGCTAATTTAACG
GTTTATCAAGCCGATCTTGAGCAATTAGAAGCGCAAAAACAACAGGCTGAGATTAACGTCGACAGTGCCCGAATCGATTT
AGGTTATACCAAAATCACCGCGCCTATGGATGGCACTGTGGTGTATTCGGCGGTGGAAGTGGGGCAAACCGTTAACGCCA
ATCAAACGACGCCGACAATCGTGGAAATGGCGCAGCTGGATACCATGACAGTTAAAGCACAGATTTCCGAAGCCGATGTC
GTCAATGTCCATCCGGGTCAAGCTGTGTATTTCACGGTACTTGGCAAACCAAATCATCCCTACCGTGGCATCTTAAGGGC
GATTGAACCTGGACCGATTTCGATGGATGGCGACGATAGTAATATGAGCTCTAGCGACTCAGACGCCATTTATTACCATG
GGTTATTCGATGTTGAAAACCCAGACCGCACCTTGCGTATCGGCATGACGGCGCAGGTATCCATAGTGTTAGCTAAGGCC
GATGATGCGCTATTAGTACCTGCGCAGATATTGCGTATAAAGCCGAGGGAGAAATCCGCGGCGCCAGAGGCTAAGTCTGG
CGAGCGTAAAGCCCGCAGTGGCCCGCAATATCAAGTGCCTGTGTTAGTTGATAACCAAGTGCAATATGTGGATGTCACGG
TTGGGATTAACAACAAAATCAATGCCGAAATCATCGCGGGCTTGAAGGAGGGCGATCAGGTCGTGCTTGGCATGCCTTCC
GCCGATGATGGCAGCAGCAAATTCCGTCGTCCACCTAGCGTGAGGTTTTAA

Upstream 100 bases:

>100_bases
TAACGACACTTCCTTCGTCTACTGTCTTTGGCTAACTCTATATACTGTTGGAACAATTTCTGTTCCTTAGCAAGATGAAT
GCTTGCTGTCTTTAGCATTG

Downstream 100 bases:

>100_bases
CAGTGACAACGGCATTACTTGAAATTTCAGGTTGTTATCGCACTTTTCAGGCGGGTGAGCAGCAACTTACTGTGCTGAAA
GATGTGAACCTGTCGATTGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPAYVTEAVRRGDIENSVLANGMLQASKLVSVGAQVSGQILS
LPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQFRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLT
VYQADLEQLEAQKQQAEINVDSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVENPDRTLRIGMTAQVSIVLAKA
DDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVPVLVDNQVQYVDVTVGINNKINAEIIAGLKEGDQVVLGMPS
ADDGSSKFRRPPSVRF

Sequences:

>Translated_416_residues
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPAYVTEAVRRGDIENSVLANGMLQASKLVSVGAQVSGQILS
LPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQFRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLT
VYQADLEQLEAQKQQAEINVDSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVENPDRTLRIGMTAQVSIVLAKA
DDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVPVLVDNQVQYVDVTVGINNKINAEIIAGLKEGDQVVLGMPS
ADDGSSKFRRPPSVRF
>Mature_416_residues
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPAYVTEAVRRGDIENSVLANGMLQASKLVSVGAQVSGQILS
LPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQFRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLT
VYQADLEQLEAQKQQAEINVDSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVENPDRTLRIGMTAQVSIVLAKA
DDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVPVLVDNQVQYVDVTVGINNKINAEIIAGLKEGDQVVLGMPS
ADDGSSKFRRPPSVRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=391, Percent_Identity=36.3171355498721, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI87082036, Length=198, Percent_Identity=29.7979797979798, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789665, Length=308, Percent_Identity=26.2987012987013, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786668, Length=333, Percent_Identity=27.3273273273273, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789929, Length=267, Percent_Identity=25.8426966292135, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44990; Mature: 44990

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPAYVTEAVRRGDIENSVLANG
CCCCCCCCCCCCCEEEEEECCEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
MLQASKLVSVGAQVSGQILSLPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQ
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHH
FRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLTVYQADLEQLEAQKQQAEINV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHEEEC
DSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
CCEEEEECEEEEECCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHCEEEEEEEECCCCE
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVEN
EEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEEEEEECCC
PDRTLRIGMTAQVSIVLAKADDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVP
CCCEEEEECEEEEEEEEEECCCCEECCHHHEEECCCCCCCCCCCCCCCHHHCCCCCEEEE
VLVDNQVQYVDVTVGINNKINAEIIAGLKEGDQVVLGMPSADDGSSKFRRPPSVRF
EEECCCEEEEEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPAYVTEAVRRGDIENSVLANG
CCCCCCCCCCCCCEEEEEECCEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
MLQASKLVSVGAQVSGQILSLPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQ
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHH
FRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLTVYQADLEQLEAQKQQAEINV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHEEEC
DSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
CCEEEEECEEEEECCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHCEEEEEEEECCCCE
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVEN
EEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEEEEEECCC
PDRTLRIGMTAQVSIVLAKADDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVP
CCCEEEEECEEEEEEEEEECCCCEECCHHHEEECCCCCCCCCCCCCCCHHHCCCCCEEEE
VLVDNQVQYVDVTVGINNKINAEIIAGLKEGDQVVLGMPSADDGSSKFRRPPSVRF
EEECCCEEEEEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]