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The map label for this gene is phsA [H]

Identifier: 160873677

GI number: 160873677

Start: 642334

End: 644628

Strand: Direct

Name: phsA [H]

Synonym: Sbal195_0555

Alternate gene names: 160873677

Gene position: 642334-644628 (Clockwise)

Preceding gene: 160873676

Following gene: 160873678

Centisome position: 12.01

GC content: 51.59

Gene sequence:

>2295_bases
ATGGAGGTAACCATGATTGAGCTTAATAGGCGAACCTTTTTAAAGGGAGCAGGAGCGAGCAGCGCCACGTGTGCGCTGGC
CAGTCTGCTGCCGGGCAGCTTAGCGGCATTAGAGAATAAGCAGCTAAAGGGCATGGGCAAAGACATTGCCAGTATTTGTG
AAATGTGTTCGACCCGTTGCCCGATTTCGGCGCGGGTGATTGAGGGCAAAAACGTCTTCATTAGTGGCAACAAGGCCGCT
AAATCCTTTGGCGGTAAAGTGTGTGCCCGTGGCGGCGCAGGCCATAGTTTATTGTACGATCCACAGCGTATCGTTAAACC
GTTAAAGCGGGTCGGTGAGCGCGGCGAAGGCAAATGGGCTGAGATCAGTTGGGATGAGGCTTATAAATTAATCGCTGAAA
ACCTCAATAAAATCAAAAAAGAACATGGCCCAGAAGCCGTGGCATTCTCGTCAAAATCTGGCTCACAAGAAAAGCATTTG
TTTCACCTTGCGACCGCCTTTGGTAGTCCAAATACTTTTACCCACGCGTCGACGTGCCCCGGCGGTTATGAAATTGCCGC
TAAGGCCATCTTTGGCACTAAGGTCAAACGAGATTTAAGTAACTCTAAATACATCATTAACTTCGGCCATAACCTGTACG
AAGGCATCAATATGTCCGAAACCCGCGGCATGATGGCGGCGCAAATGGACAAAGGTGCCAAGCTGGTGGTCTTCGAGCCA
AGATTCTCCGTCGTGGCCGATAAAGCCGATGAATGGTATGCCATTCGCCCCGGAACCGACGTTGCCGTCGCCTTGGCGTT
ATGCCATGTATTGATTGAAGATAATCTTTACGACAAGGCATTCGTCGCGCGCTATGTGGAAGGCTTCGAAGCCTTTGCCG
CCGAAGTGAAAGCTTATACCCCTGAATGGGCCGAAACCATCTCCGATGTGCCTGCCAAAGACATTCGTCGTATCGCCCAC
GAATATGCCGCTAAAGCGCCCCATGCCGTGGTCGATTTTGGTCACAGAGCTACCTTTACCACCGAAGAGTTTGAGATGCG
CCGCGCCCTGTACGCCGCCAACGTCTTGATTGGTAATATTGAGCGCAAGGGGGGGATTTATATCGGCCAGAAACCCGGTG
ATTACAACAAGCTTGCCGGTGAAGCGGTTGCGCCCGTATTGGCAAAACCTGGCGTGAAAGACATGCCAAAACCAGCAGCG
AAACGTATCGATCAAGTGGAAGAACAGTACGCCATGATGTGGACATCGGGCGGTGTTTACCAAACCATTCTCGATGCCAC
CTTAAGCGCTGTGCCCTACCAGTTACACGGCTGGGTGATGTCACGCACTAACCCAATGCAAACCATGACAGACAGGGCGC
GCGTTGTTGAAGCGATGAAAAAGCTCGACTTTATCGCCGTGTGTGATGTGTATATCAGCGAAACCGCCGCCTATGCTGAC
GTGATTTTACCTGAGTCAACTTATCTTGAACGTGATGAAGAAATTGCTGATAAGTCAGGTAAAAATCCCGCCTATTATGT
GCGCCAACGTGTGGTTGAAACCTTAGGTGATACTAAGCCTTCTTGGCAGATCTTTAAGGATGTGGGTGTGGCTATGGGCC
TTGGCGAGTTCTATCCATGGGAAAACATGGAAACCCTGCAAATGCTGCAGGTGAACCGCGACACTGACCTGCTGCGCCGT
ATCAAAAACGAAGGTTTTGTGAGTTTTGGTAAACCGCTCATGCTGCGCGAAGCCAAGATGGTGAATGATTTCACTCAAGC
CTATCCAAGCGCACGGCCAATGGATGACGACGGCACTTACGCCAGTGCACTCACCTTTAAAACCCCGAGTGGCAAGATTG
AACTCACCTCCGCGAAAGTCGAAGCCATGGCACCGGGCCGTGGGGTGATCAAGTTCCGCGAAGTACATCTTAAAAAAGCA
GATGAGCTGTACTTTATCCAAGGCAAAGTGGCGGTGCACACCAATGGCGCCACCCACAATGTGCCTATGCTCGCTAACCT
GATGTCGGATAATGCTGTGTGGGTTCACCCCGTCACTGCAGGCAAGTTAGGGATCAGCAATGGCGACCCAATCCGCCTCA
CCAGCAGTGTTGGCACAGAAGAAGGCCATGCCTTAGTCACGCCGGGCATTCGCCAAGATACTGTGTTTGCTTACATGGGC
TTTGGTTCTAAAAACAAAGAGTTGGTCAGAGCAACAGGCAAAGGTATCCACTGCGGTAACTTACTGCCGCACGTGACGGC
TCCTGTGTGTGGCATGACAGTGCACACCACGGGCGTCACGCTGGCAAAGCGCTAA

Upstream 100 bases:

>100_bases
CGCTGTTTTAACGCTTTATAACTAAAAAAGTAATCAAATCAGTGAGCAAACCTCTGCTCGATGGCGCCAAAAATGGCGAC
TGACGGGTGGGATGAGCGTG

Downstream 100 bases:

>100_bases
TCGGAGGCTTAAATGAATAAACGATATGTAATGGTGCACGACGAGAATAAGTGCATCGGCTGCCAAGCTTGTAACGTGGC
GTGTCGCAGTGAAAATGGCG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MEVTMIELNRRTFLKGAGASSATCALASLLPGSLAALENKQLKGMGKDIASICEMCSTRCPISARVIEGKNVFISGNKAA
KSFGGKVCARGGAGHSLLYDPQRIVKPLKRVGERGEGKWAEISWDEAYKLIAENLNKIKKEHGPEAVAFSSKSGSQEKHL
FHLATAFGSPNTFTHASTCPGGYEIAAKAIFGTKVKRDLSNSKYIINFGHNLYEGINMSETRGMMAAQMDKGAKLVVFEP
RFSVVADKADEWYAIRPGTDVAVALALCHVLIEDNLYDKAFVARYVEGFEAFAAEVKAYTPEWAETISDVPAKDIRRIAH
EYAAKAPHAVVDFGHRATFTTEEFEMRRALYAANVLIGNIERKGGIYIGQKPGDYNKLAGEAVAPVLAKPGVKDMPKPAA
KRIDQVEEQYAMMWTSGGVYQTILDATLSAVPYQLHGWVMSRTNPMQTMTDRARVVEAMKKLDFIAVCDVYISETAAYAD
VILPESTYLERDEEIADKSGKNPAYYVRQRVVETLGDTKPSWQIFKDVGVAMGLGEFYPWENMETLQMLQVNRDTDLLRR
IKNEGFVSFGKPLMLREAKMVNDFTQAYPSARPMDDDGTYASALTFKTPSGKIELTSAKVEAMAPGRGVIKFREVHLKKA
DELYFIQGKVAVHTNGATHNVPMLANLMSDNAVWVHPVTAGKLGISNGDPIRLTSSVGTEEGHALVTPGIRQDTVFAYMG
FGSKNKELVRATGKGIHCGNLLPHVTAPVCGMTVHTTGVTLAKR

Sequences:

>Translated_764_residues
MEVTMIELNRRTFLKGAGASSATCALASLLPGSLAALENKQLKGMGKDIASICEMCSTRCPISARVIEGKNVFISGNKAA
KSFGGKVCARGGAGHSLLYDPQRIVKPLKRVGERGEGKWAEISWDEAYKLIAENLNKIKKEHGPEAVAFSSKSGSQEKHL
FHLATAFGSPNTFTHASTCPGGYEIAAKAIFGTKVKRDLSNSKYIINFGHNLYEGINMSETRGMMAAQMDKGAKLVVFEP
RFSVVADKADEWYAIRPGTDVAVALALCHVLIEDNLYDKAFVARYVEGFEAFAAEVKAYTPEWAETISDVPAKDIRRIAH
EYAAKAPHAVVDFGHRATFTTEEFEMRRALYAANVLIGNIERKGGIYIGQKPGDYNKLAGEAVAPVLAKPGVKDMPKPAA
KRIDQVEEQYAMMWTSGGVYQTILDATLSAVPYQLHGWVMSRTNPMQTMTDRARVVEAMKKLDFIAVCDVYISETAAYAD
VILPESTYLERDEEIADKSGKNPAYYVRQRVVETLGDTKPSWQIFKDVGVAMGLGEFYPWENMETLQMLQVNRDTDLLRR
IKNEGFVSFGKPLMLREAKMVNDFTQAYPSARPMDDDGTYASALTFKTPSGKIELTSAKVEAMAPGRGVIKFREVHLKKA
DELYFIQGKVAVHTNGATHNVPMLANLMSDNAVWVHPVTAGKLGISNGDPIRLTSSVGTEEGHALVTPGIRQDTVFAYMG
FGSKNKELVRATGKGIHCGNLLPHVTAPVCGMTVHTTGVTLAKR
>Mature_764_residues
MEVTMIELNRRTFLKGAGASSATCALASLLPGSLAALENKQLKGMGKDIASICEMCSTRCPISARVIEGKNVFISGNKAA
KSFGGKVCARGGAGHSLLYDPQRIVKPLKRVGERGEGKWAEISWDEAYKLIAENLNKIKKEHGPEAVAFSSKSGSQEKHL
FHLATAFGSPNTFTHASTCPGGYEIAAKAIFGTKVKRDLSNSKYIINFGHNLYEGINMSETRGMMAAQMDKGAKLVVFEP
RFSVVADKADEWYAIRPGTDVAVALALCHVLIEDNLYDKAFVARYVEGFEAFAAEVKAYTPEWAETISDVPAKDIRRIAH
EYAAKAPHAVVDFGHRATFTTEEFEMRRALYAANVLIGNIERKGGIYIGQKPGDYNKLAGEAVAPVLAKPGVKDMPKPAA
KRIDQVEEQYAMMWTSGGVYQTILDATLSAVPYQLHGWVMSRTNPMQTMTDRARVVEAMKKLDFIAVCDVYISETAAYAD
VILPESTYLERDEEIADKSGKNPAYYVRQRVVETLGDTKPSWQIFKDVGVAMGLGEFYPWENMETLQMLQVNRDTDLLRR
IKNEGFVSFGKPLMLREAKMVNDFTQAYPSARPMDDDGTYASALTFKTPSGKIELTSAKVEAMAPGRGVIKFREVHLKKA
DELYFIQGKVAVHTNGATHNVPMLANLMSDNAVWVHPVTAGKLGISNGDPIRLTSSVGTEEGHALVTPGIRQDTVFAYMG
FGSKNKELVRATGKGIHCGNLLPHVTAPVCGMTVHTTGVTLAKR

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=781, Percent_Identity=25.6081946222791, Blast_Score=183, Evalue=5e-47,
Organism=Escherichia coli, GI171474008, Length=775, Percent_Identity=25.4193548387097, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1787870, Length=771, Percent_Identity=26.0700389105058, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI3868721, Length=517, Percent_Identity=28.8201160541586, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI145693196, Length=672, Percent_Identity=23.6607142857143, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787231, Length=698, Percent_Identity=22.0630372492837, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI87081994, Length=696, Percent_Identity=23.5632183908046, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788534, Length=583, Percent_Identity=21.6123499142367, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI3868720, Length=309, Percent_Identity=24.9190938511327, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 83624; Mature: 83624

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTMIELNRRTFLKGAGASSATCALASLLPGSLAALENKQLKGMGKDIASICEMCSTRC
CEEEEEEECCCEEEECCCCCHHHHHHHHHCCCHHHHHCCHHHHCCCHHHHHHHHHHHCCC
PISARVIEGKNVFISGNKAAKSFGGKVCARGGAGHSLLYDPQRIVKPLKRVGERGEGKWA
CCEEEEEECCEEEEECCHHHHHCCCCEEECCCCCCCCEECHHHHHHHHHHHCCCCCCCEE
EISWDEAYKLIAENLNKIKKEHGPEAVAFSSKSGSQEKHLFHLATAFGSPNTFTHASTCP
ECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEEHHHCCCCCCCCCCCCCC
GGYEIAAKAIFGTKVKRDLSNSKYIINFGHNLYEGINMSETRGMMAAQMDKGAKLVVFEP
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHCCHHHHHHCCCCEEEEECC
RFSVVADKADEWYAIRPGTDVAVALALCHVLIEDNLYDKAFVARYVEGFEAFAAEVKAYT
CCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PEWAETISDVPAKDIRRIAHEYAAKAPHAVVDFGHRATFTTEEFEMRRALYAANVLIGNI
CHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHEECE
ERKGGIYIGQKPGDYNKLAGEAVAPVLAKPGVKDMPKPAAKRIDQVEEQYAMMWTSGGVY
ECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
QTILDATLSAVPYQLHGWVMSRTNPMQTMTDRARVVEAMKKLDFIAVCDVYISETAAYAD
HHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
VILPESTYLERDEEIADKSGKNPAYYVRQRVVETLGDTKPSWQIFKDVGVAMGLGEFYPW
EECCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
ENMETLQMLQVNRDTDLLRRIKNEGFVSFGKPLMLREAKMVNDFTQAYPSARPMDDDGTY
CCCCHHHHHHCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
ASALTFKTPSGKIELTSAKVEAMAPGRGVIKFREVHLKKADELYFIQGKVAVHTNGATHN
EEEEEEECCCCCEEEEECEEEEECCCCCCEEEHHHHHCCCCCEEEEEEEEEEEECCCCCC
VPMLANLMSDNAVWVHPVTAGKLGISNGDPIRLTSSVGTEEGHALVTPGIRQDTVFAYMG
CHHHHHHHCCCCEEEEEECCCCEECCCCCCEEEEECCCCCCCCEEECCCCCCCEEEEEEC
FGSKNKELVRATGKGIHCGNLLPHVTAPVCGMTVHTTGVTLAKR
CCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCEEEEECCEEEECC
>Mature Secondary Structure
MEVTMIELNRRTFLKGAGASSATCALASLLPGSLAALENKQLKGMGKDIASICEMCSTRC
CEEEEEEECCCEEEECCCCCHHHHHHHHHCCCHHHHHCCHHHHCCCHHHHHHHHHHHCCC
PISARVIEGKNVFISGNKAAKSFGGKVCARGGAGHSLLYDPQRIVKPLKRVGERGEGKWA
CCEEEEEECCEEEEECCHHHHHCCCCEEECCCCCCCCEECHHHHHHHHHHHCCCCCCCEE
EISWDEAYKLIAENLNKIKKEHGPEAVAFSSKSGSQEKHLFHLATAFGSPNTFTHASTCP
ECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEEHHHCCCCCCCCCCCCCC
GGYEIAAKAIFGTKVKRDLSNSKYIINFGHNLYEGINMSETRGMMAAQMDKGAKLVVFEP
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHCCHHHHHHCCCCEEEEECC
RFSVVADKADEWYAIRPGTDVAVALALCHVLIEDNLYDKAFVARYVEGFEAFAAEVKAYT
CCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PEWAETISDVPAKDIRRIAHEYAAKAPHAVVDFGHRATFTTEEFEMRRALYAANVLIGNI
CHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHEECE
ERKGGIYIGQKPGDYNKLAGEAVAPVLAKPGVKDMPKPAAKRIDQVEEQYAMMWTSGGVY
ECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
QTILDATLSAVPYQLHGWVMSRTNPMQTMTDRARVVEAMKKLDFIAVCDVYISETAAYAD
HHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
VILPESTYLERDEEIADKSGKNPAYYVRQRVVETLGDTKPSWQIFKDVGVAMGLGEFYPW
EECCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
ENMETLQMLQVNRDTDLLRRIKNEGFVSFGKPLMLREAKMVNDFTQAYPSARPMDDDGTY
CCCCHHHHHHCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
ASALTFKTPSGKIELTSAKVEAMAPGRGVIKFREVHLKKADELYFIQGKVAVHTNGATHN
EEEEEEECCCCCEEEEECEEEEECCCCCCEEEHHHHHCCCCCEEEEEEEEEEEECCCCCC
VPMLANLMSDNAVWVHPVTAGKLGISNGDPIRLTSSVGTEEGHALVTPGIRQDTVFAYMG
CHHHHHHHCCCCEEEEEECCCCEECCCCCCEEEEECCCCCCCCEEECCCCCCCEEEEEEC
FGSKNKELVRATGKGIHCGNLLPHVTAPVCGMTVHTTGVTLAKR
CCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]