Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 160873595

GI number: 160873595

Start: 541889

End: 543025

Strand: Direct

Name: nemA [H]

Synonym: Sbal195_0473

Alternate gene names: 160873595

Gene position: 541889-543025 (Clockwise)

Preceding gene: 160873592

Following gene: 160873596

Centisome position: 10.13

GC content: 49.6

Gene sequence:

>1137_bases
ATGAGTCTTTTCATGTCTTATAAATTAGCGTCACTGGCATTAAACAATCGCATTGTGATGGCCCCTATGACTCGGGCGCG
TACTACGCAGCCAGGCAATATTCCGAATGATCTGATGGCGCAATATTATGCCCAGCGCGCCTCGGCGGGGTTAATCATCA
CAGAAGCGACACAAATCTCCGATGACAGCCAAGGTTATTCATTTACGCCGGGCGTGTATACCGCTGAGCAAATTGAGGGC
TGGAAAAAAGTCACAGCGGCAGTGCATCAGGCTGGTGGTAAAATCTTCAATCAAATTTGGCATGTTGGCCGTGTATCGCA
CCCGATTTTTCAGCAGGGTAATGCCCCGATTGCGCCATCAGCTATTGCGCCCGTTGGCACTAAAGTATGGATTGTGGATG
AAGCGCATCCTGAAGGGCAAATGGTGGATTGCCCAGAACCTAGGGCTATGGTGCAAGCCGATATCGACCGCGTTGTGGCC
GATTTTGCACAGGCGGGAGTGAATGCGGTTGCGGCGGGTTTTGATGGCATCGAAATTCACGGCGGCAATGGTTATCTGAT
TGACCAGTTTTTACGCACCAATTCAAACCACAGAACTGATGCCTACGGCGGTTCTCAGGAAAATCGCATTCGTTTTTTAC
TCGAAATTGTCCAAGCCGTGAGTGCGAAAATTGGTGCCGATAAAGTCGGGGTTCGCCTCGCCCCTTACGTGACGTTTAAA
GATATGGCGTGTCCTGAAATTGTAGACACTATTCTGTTAGCGGCTAAGCATTTATCGGCATTGGGCGTGGCATATTTACA
TCTTTCAGAGGCGGATTGGGATGATGCGCCGCAGGTGCCAGAATCCTTTAGAGTCGAGCTGCGTAACGTCTTTAAGGGCA
GCATTATTGTTGCGGGTCGCTATGATGTGGCGCGTGCCAATGAAGTGATTGATAACGGTTATGCGGACTTAGTTGCCTTC
GGTCGACCTTTTATTGCGAATCCTGATTTGCCTTACCGTTTAATGCATCAATTGCCTTTATCTGCATTCGACAAAGGGCC
CTTGTTTGGCGGCAGTGCGGCGGGTTATACCGATTATCCTACACACAACGAAGCATTGAGAGCCGTCATCCGTTCGGATG
ACTATGAGGTGGCATAA

Upstream 100 bases:

>100_bases
TTTGAAAATGATTTTAATAATAGCCTGATTATCAATATAGATTAACTAATGCACAATTGCACCACTGATGTTTCATCTTA
ATTTCTGGTTCGGAGTGTGT

Downstream 100 bases:

>100_bases
TGGATAGGGTTAACCGTTTATCTGTGCAGCGATATCCCGCATGGCTCAGTCTAAGCTTATGGTTTTCAGGCATTATAGGT
ATCGCTATTGTGGGTTTTAG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSLFMSYKLASLALNNRIVMAPMTRARTTQPGNIPNDLMAQYYAQRASAGLIITEATQISDDSQGYSFTPGVYTAEQIEG
WKKVTAAVHQAGGKIFNQIWHVGRVSHPIFQQGNAPIAPSAIAPVGTKVWIVDEAHPEGQMVDCPEPRAMVQADIDRVVA
DFAQAGVNAVAAGFDGIEIHGGNGYLIDQFLRTNSNHRTDAYGGSQENRIRFLLEIVQAVSAKIGADKVGVRLAPYVTFK
DMACPEIVDTILLAAKHLSALGVAYLHLSEADWDDAPQVPESFRVELRNVFKGSIIVAGRYDVARANEVIDNGYADLVAF
GRPFIANPDLPYRLMHQLPLSAFDKGPLFGGSAAGYTDYPTHNEALRAVIRSDDYEVA

Sequences:

>Translated_378_residues
MSLFMSYKLASLALNNRIVMAPMTRARTTQPGNIPNDLMAQYYAQRASAGLIITEATQISDDSQGYSFTPGVYTAEQIEG
WKKVTAAVHQAGGKIFNQIWHVGRVSHPIFQQGNAPIAPSAIAPVGTKVWIVDEAHPEGQMVDCPEPRAMVQADIDRVVA
DFAQAGVNAVAAGFDGIEIHGGNGYLIDQFLRTNSNHRTDAYGGSQENRIRFLLEIVQAVSAKIGADKVGVRLAPYVTFK
DMACPEIVDTILLAAKHLSALGVAYLHLSEADWDDAPQVPESFRVELRNVFKGSIIVAGRYDVARANEVIDNGYADLVAF
GRPFIANPDLPYRLMHQLPLSAFDKGPLFGGSAAGYTDYPTHNEALRAVIRSDDYEVA
>Mature_377_residues
SLFMSYKLASLALNNRIVMAPMTRARTTQPGNIPNDLMAQYYAQRASAGLIITEATQISDDSQGYSFTPGVYTAEQIEGW
KKVTAAVHQAGGKIFNQIWHVGRVSHPIFQQGNAPIAPSAIAPVGTKVWIVDEAHPEGQMVDCPEPRAMVQADIDRVVAD
FAQAGVNAVAAGFDGIEIHGGNGYLIDQFLRTNSNHRTDAYGGSQENRIRFLLEIVQAVSAKIGADKVGVRLAPYVTFKD
MACPEIVDTILLAAKHLSALGVAYLHLSEADWDDAPQVPESFRVELRNVFKGSIIVAGRYDVARANEVIDNGYADLVAFG
RPFIANPDLPYRLMHQLPLSAFDKGPLFGGSAAGYTDYPTHNEALRAVIRSDDYEVA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=47.5138121546961, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=30.1449275362319, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17559802, Length=203, Percent_Identity=32.512315270936, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=373, Percent_Identity=27.0777479892761, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=215, Percent_Identity=31.6279069767442, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=352, Percent_Identity=26.7045454545455, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=360, Percent_Identity=26.9444444444444, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=267, Percent_Identity=27.3408239700375, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=385, Percent_Identity=38.7012987012987, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321973, Length=392, Percent_Identity=37.7551020408163, Blast_Score=229, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41106; Mature: 40975

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFMSYKLASLALNNRIVMAPMTRARTTQPGNIPNDLMAQYYAQRASAGLIITEATQIS
CCCCHHHHHHHHHHCCEEEEECCHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
DDSQGYSFTPGVYTAEQIEGWKKVTAAVHQAGGKIFNQIWHVGRVSHPIFQQGNAPIAPS
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
AIAPVGTKVWIVDEAHPEGQMVDCPEPRAMVQADIDRVVADFAQAGVNAVAAGFDGIEIH
CCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEEE
GGNGYLIDQFLRTNSNHRTDAYGGSQENRIRFLLEIVQAVSAKIGADKVGVRLAPYVTFK
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEEEC
DMACPEIVDTILLAAKHLSALGVAYLHLSEADWDDAPQVPESFRVELRNVFKGSIIVAGR
CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEECC
YDVARANEVIDNGYADLVAFGRPFIANPDLPYRLMHQLPLSAFDKGPLFGGSAAGYTDYP
CCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCCCCCC
THNEALRAVIRSDDYEVA
CHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLFMSYKLASLALNNRIVMAPMTRARTTQPGNIPNDLMAQYYAQRASAGLIITEATQIS
CCCHHHHHHHHHHCCEEEEECCHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
DDSQGYSFTPGVYTAEQIEGWKKVTAAVHQAGGKIFNQIWHVGRVSHPIFQQGNAPIAPS
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
AIAPVGTKVWIVDEAHPEGQMVDCPEPRAMVQADIDRVVADFAQAGVNAVAAGFDGIEIH
CCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEEE
GGNGYLIDQFLRTNSNHRTDAYGGSQENRIRFLLEIVQAVSAKIGADKVGVRLAPYVTFK
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEEEC
DMACPEIVDTILLAAKHLSALGVAYLHLSEADWDDAPQVPESFRVELRNVFKGSIIVAGR
CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEECC
YDVARANEVIDNGYADLVAFGRPFIANPDLPYRLMHQLPLSAFDKGPLFGGSAAGYTDYP
CCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCCCCCC
THNEALRAVIRSDDYEVA
CHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]