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The map label for this gene is mraW

Identifier: 160873530

GI number: 160873530

Start: 461051

End: 461992

Strand: Direct

Name: mraW

Synonym: Sbal195_0405

Alternate gene names: 160873530

Gene position: 461051-461992 (Clockwise)

Preceding gene: 160873529

Following gene: 160873531

Centisome position: 8.62

GC content: 48.41

Gene sequence:

>942_bases
ATGAGTCAAGAATTTGCCCATTTATCCGTGCTGCTAGCAGAAACGGTTGGCGGTTTAAACATCAAAGACGATGGCATCTA
TATCGATGGCACGTTTGGCCGCGGTGGTCATTCAAGACAAGTGTTGCAGCAACTCGGTGAAAATGGCCGATTGATCGCAA
TCGACCGTGACCCTCAAGCAATTGAAGCGGCAAAACAATTTGCTGACGATCCACGCTTTCAAATCGTGCATGGTGGTTTT
GGTCAATTAGCCGATTACGTCGAAGAGCTTGGGCTTGTGGGCAAAATAGATGGCGTATTACTCGATTTAGGTGTGTCTTC
ACCTCAGCTCGACGATGCCGAACGTGGCTTTAGTTTTATGCGTGATGGCCCGCTCGATATGCGCATGGATAACAGCCAAG
GCCAAACAGCTGCGCAATGGTTAGCTCGCGCAGAAATTGAGGATATGGCTTGGGTATTTAAAACCTATGGCGAAGAGAAA
AACGCTCGCCATATCGCCCGTTGCATCGCTGCCGATCGTGATAAAACGCCGTTTTTACGAACCAAAGATTTAGCCGATTT
GATCGCGCGTATCACTAAGAGTAAAGAACGCAATAAGCACCCAGCGACTCGGGTGTTTCAGGCCATTCGGATTTATATCA
ACAGTGAATTAGATCAAATTGATCAAGCGCTTGAAGGTGCGGTCAATGTACTGGCTCCCCAAGGACGCTTGTCGGTCATC
AGTTTCCATTCTTTAGAAGATCGTATCGTGAAGCGCTTTATTCGCCGCCATAGCCAAGGCGAAAGTGTGCCGCACGGTTT
TCCGGTCACTGAAGATCAAATTAACAAGTCGCGTAAGTTACGCGCCGTGGGTAAGGCGATTATGCCATCCGACGAAGAAA
TCGAACGCAATGCCAGAGCTCGCAGCTCAGTGTTACGCATTGCCGAGCGTTTAGATTACTAA

Upstream 100 bases:

>100_bases
GAGTGTCAGGAAACGATTCGAAGTGAGGAGCTTGCCAGTAACGAGCGCCTCGCGGATTTTTCACTTTAACGAGTCGCGAT
AACTAAATAAGAAGAGAGTA

Downstream 100 bases:

>100_bases
GGGGCGCACTGTGAGTAAGCCCTCGATCAATCTGCCACGGATCGTCTTACACGATTTATGGCTGCACAAATGGATCTTGT
TATTAGCTCTGCTCGTGCTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQVLQQLGENGRLIAIDRDPQAIEAAKQFADDPRFQIVHGGF
GQLADYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFMRDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEK
NARHIARCIAADRDKTPFLRTKDLADLIARITKSKERNKHPATRVFQAIRIYINSELDQIDQALEGAVNVLAPQGRLSVI
SFHSLEDRIVKRFIRRHSQGESVPHGFPVTEDQINKSRKLRAVGKAIMPSDEEIERNARARSSVLRIAERLDY

Sequences:

>Translated_313_residues
MSQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQVLQQLGENGRLIAIDRDPQAIEAAKQFADDPRFQIVHGGF
GQLADYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFMRDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEK
NARHIARCIAADRDKTPFLRTKDLADLIARITKSKERNKHPATRVFQAIRIYINSELDQIDQALEGAVNVLAPQGRLSVI
SFHSLEDRIVKRFIRRHSQGESVPHGFPVTEDQINKSRKLRAVGKAIMPSDEEIERNARARSSVLRIAERLDY
>Mature_312_residues
SQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQVLQQLGENGRLIAIDRDPQAIEAAKQFADDPRFQIVHGGFG
QLADYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFMRDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEKN
ARHIARCIAADRDKTPFLRTKDLADLIARITKSKERNKHPATRVFQAIRIYINSELDQIDQALEGAVNVLAPQGRLSVIS
FHSLEDRIVKRFIRRHSQGESVPHGFPVTEDQINKSRKLRAVGKAIMPSDEEIERNARARSSVLRIAERLDY

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=38.1231671554252, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=39.6907216494845, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=64.968152866242, Blast_Score=418, Evalue=1e-118,
Organism=Drosophila melanogaster, GI62472493, Length=327, Percent_Identity=38.2262996941896, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI28571637, Length=327, Percent_Identity=38.2262996941896, Blast_Score=187, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_SHEB2 (B8E4L2)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002356376.1
- ProteinModelPortal:   B8E4L2
- SMR:   B8E4L2
- GeneID:   7089557
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_0419
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35031; Mature: 34900

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 80-80 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQVLQQLGENGRLIAIDRDPQA
CCCHHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IEAAKQFADDPRFQIVHGGFGQLADYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFM
HHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHCHHHH
RDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEKNARHIARCIAADRDKTPFLR
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
TKDLADLIARITKSKERNKHPATRVFQAIRIYINSELDQIDQALEGAVNVLAPQGRLSVI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEE
SFHSLEDRIVKRFIRRHSQGESVPHGFPVTEDQINKSRKLRAVGKAIMPSDEEIERNARA
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RSSVLRIAERLDY
HHHHHHHHHHHCC
>Mature Secondary Structure 
SQEFAHLSVLLAETVGGLNIKDDGIYIDGTFGRGGHSRQVLQQLGENGRLIAIDRDPQA
CCHHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IEAAKQFADDPRFQIVHGGFGQLADYVEELGLVGKIDGVLLDLGVSSPQLDDAERGFSFM
HHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHCHHHH
RDGPLDMRMDNSQGQTAAQWLARAEIEDMAWVFKTYGEEKNARHIARCIAADRDKTPFLR
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
TKDLADLIARITKSKERNKHPATRVFQAIRIYINSELDQIDQALEGAVNVLAPQGRLSVI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEE
SFHSLEDRIVKRFIRRHSQGESVPHGFPVTEDQINKSRKLRAVGKAIMPSDEEIERNARA
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RSSVLRIAERLDY
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA