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The map label for this gene is leuB [H]

Identifier: 160873523

GI number: 160873523

Start: 452653

End: 453747

Strand: Direct

Name: leuB [H]

Synonym: Sbal195_0398

Alternate gene names: 160873523

Gene position: 452653-453747 (Clockwise)

Preceding gene: 160873522

Following gene: 160873524

Centisome position: 8.47

GC content: 51.32

Gene sequence:

>1095_bases
ATGAGTTATCAAGTAGCAGTATTAGCAGGGGACGGGATCGGTCCAGAAGTGATGGCAGAGGCGCGTAAAGTGCTGCGTGA
AGTGGAAGCGCGATTCGATTTAATTATCGAATATACACAATACGATGTCGGCGGTATTGCCATCGATAACCACGGTTGCC
CGCTACCCGATGCCACGCTAAAAGGTTGTGAAGCGGCCGATGCGATTCTATTTGGTTCAGTCGGTGGCCCTAAGTGGGAA
AAGTTACCGCCAAATGAACAACCTGAGCGCGGCGCGCTATTGCCTCTGCGTGGTCACTTCGAACTGTTTTGCAATCTGCG
CCCAGCCAAACTGCACGATGGCCTAGAGCATATGTCACCGCTGCGTAGCGATATCTCTGCCCGTGGCTTTGATGTTTTAT
GTGTGCGTGAGTTAACCGGTGGCATTTATTTTGGTAAGCCAAAGGGCCGCCAAGGTGAAGGCGAGAATGAAGAAGCCTTC
GATACCATGCGTTATAGCCGCCGCGAAATTAGCCGTATCGCCCGCATCGCCTTTGAAGCGGCCCGTGGTCGTCGTAAAAA
AGTCACCTCAGTGGATAAAGCCAACGTACTGGCTTGTTCAGTGTTGTGGCGCCAAGTGGTTGAAGAAGTGGCGGTTGATT
TTCCCGATGTCGAGCTGGAACATATCTATATCGATAACGCGACCATGCAACTGCTGCGTCGCCCCGATGAGTTCGATGTT
ATGTTATGTTCTAACCTGTTCGGTGACATTTTATCCGACGAAATCGCCATGTTAACGGGCTCTATGGGCTTGTTGTCCTC
GGCGAGCATGAACAGCAGTGGTTTTGGTTTATTCGAACCAGCAGGTGGCAGTGCGCCGGATATCGCAGGTAAAGGGATAG
CCAACCCAGTCGCGCAAATTCTGTCGGCAGCATTAATGTTGCGTCATAGCTTGAAACAAGAAGAAGCGGCGAGTGCGATT
GAGCGCGCCGTGAGCAAAGCCTTGAACTCTGGCTATTTAACTGGCGAGCTGTTGAGCAGCGACAAGCGTAGCGAGGCTAA
ATCGACGGCAGAAATGGGTGACTTTATCGCCAATGCTATAAAGGAAGGTGTGTAA

Upstream 100 bases:

>100_bases
CAAAAGAATCGAGAGCTAGGCGGCGTCTAGTCGCTAAAAGCTTCCAAAGAATAGCAAGGTCGAGCATAAAGCATTCACAT
TTAAAGGAGTTGGCTGGCGT

Downstream 100 bases:

>100_bases
TGACGAACGCAAAGACCTTGTACCAAAAAGTATGGGATGCACATATCGTTGCTGCTCCCGAGGGCGAAGCGCCGGTCATT
TATGTCGACAGACATTTAGT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSYQVAVLAGDGIGPEVMAEARKVLREVEARFDLIIEYTQYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
KLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSPLRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAF
DTMRYSRREISRIARIAFEAARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSSGFGLFEPAGGSAPDIAGKGIANPVAQILSAALMLRHSLKQEEAASAI
ERAVSKALNSGYLTGELLSSDKRSEAKSTAEMGDFIANAIKEGV

Sequences:

>Translated_364_residues
MSYQVAVLAGDGIGPEVMAEARKVLREVEARFDLIIEYTQYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
KLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSPLRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAF
DTMRYSRREISRIARIAFEAARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSSGFGLFEPAGGSAPDIAGKGIANPVAQILSAALMLRHSLKQEEAASAI
ERAVSKALNSGYLTGELLSSDKRSEAKSTAEMGDFIANAIKEGV
>Mature_363_residues
SYQVAVLAGDGIGPEVMAEARKVLREVEARFDLIIEYTQYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWEK
LPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSPLRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAFD
TMRYSRREISRIARIAFEAARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDVM
LCSNLFGDILSDEIAMLTGSMGLLSSASMNSSGFGLFEPAGGSAPDIAGKGIANPVAQILSAALMLRHSLKQEEAASAIE
RAVSKALNSGYLTGELLSSDKRSEAKSTAEMGDFIANAIKEGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=32.0916905444126, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=30.1492537313433, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4758582, Length=335, Percent_Identity=30.1492537313433, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI28178821, Length=330, Percent_Identity=30.6060606060606, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178816, Length=330, Percent_Identity=30.6060606060606, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=162, Percent_Identity=40.1234567901235, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=68.5950413223141, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1788101, Length=312, Percent_Identity=38.1410256410256, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1787381, Length=306, Percent_Identity=26.797385620915, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=32.258064516129, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=330, Percent_Identity=30.9090909090909, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=30.2052785923754, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=317, Percent_Identity=32.807570977918, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=43.5135135135135, Blast_Score=269, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=344, Percent_Identity=34.8837209302326, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6324709, Length=340, Percent_Identity=32.0588235294118, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=346, Percent_Identity=29.4797687861272, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=35.4285714285714, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=35.4285714285714, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI161078633, Length=333, Percent_Identity=31.5315315315315, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24650122, Length=333, Percent_Identity=31.5315315315315, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078635, Length=333, Percent_Identity=31.5315315315315, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078637, Length=333, Percent_Identity=31.5315315315315, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161078639, Length=329, Percent_Identity=31.6109422492401, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24661184, Length=332, Percent_Identity=29.8192771084337, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281362242, Length=337, Percent_Identity=27.0029673590504, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648872, Length=337, Percent_Identity=27.0029673590504, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20130355, Length=336, Percent_Identity=25.8928571428571, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39448; Mature: 39317

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQVAVLAGDGIGPEVMAEARKVLREVEARFDLIIEYTQYDVGGIAIDNHGCPLPDATL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEKLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSP
CCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHH
LRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAFDTMRYSRREISRIARIAFEA
HHHHHHCCCCCEEEHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSSGFGLFEPAGGSAPDIAGKGIANPVAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHH
LSAALMLRHSLKQEEAASAIERAVSKALNSGYLTGELLSSDKRSEAKSTAEMGDFIANAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
KEGV
HHCC
>Mature Secondary Structure 
SYQVAVLAGDGIGPEVMAEARKVLREVEARFDLIIEYTQYDVGGIAIDNHGCPLPDATL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEKLPPNEQPERGALLPLRGHFELFCNLRPAKLHDGLEHMSP
CCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHH
LRSDISARGFDVLCVRELTGGIYFGKPKGRQGEGENEEAFDTMRYSRREISRIARIAFEA
HHHHHHCCCCCEEEHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARGRRKKVTSVDKANVLACSVLWRQVVEEVAVDFPDVELEHIYIDNATMQLLRRPDEFDV
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNSSGFGLFEPAGGSAPDIAGKGIANPVAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHH
LSAALMLRHSLKQEEAASAIERAVSKALNSGYLTGELLSSDKRSEAKSTAEMGDFIANAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
KEGV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]