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The map label for this gene is spoT [H]

Identifier: 160873483

GI number: 160873483

Start: 405712

End: 407817

Strand: Reverse

Name: spoT [H]

Synonym: Sbal195_0358

Alternate gene names: 160873483

Gene position: 407817-405712 (Counterclockwise)

Preceding gene: 160873484

Following gene: 160873482

Centisome position: 7.63

GC content: 51.28

Gene sequence:

>2106_bases
TTGTATCTGTTTGAAGGTCTAAAGGAGTCTGCTTCCGGTTACTTAGAACCCGAACAGGTAGAATTGCTCAAGCAGGCGTA
CCAGATTGCGCGCGATGCCCACGAAGGGCAAATGCGCACGAGTGGCGAACCTTATATTACTCATCCGGTTGCGGTTGCCC
GCATCCTTGCTGACATGCGTCTTGATCATGAGACGCTGATGGCAGCACTGCTCCACGACACTATCGAAGATACTCATGTC
ACCAAAGACGATTTAGCCGAACTATTCGGTGTCGCGGTGGCCGAGCTCGTCGAAGGCGTGTCAAAACTCGATAAGCTTAA
ATTTCGCGACAAGAAAGAAGCTCAGGCTGAAAACTTCCGTAAAATGATGATGGCGATGACGCAGGATATCCGCGTTATCC
TCATCAAGCTTGCCGATAGAACCCACAACATGCGTACCTTAGGCGCCCTGCGTCCCGACAAACGTCGCCGTATCGCCCGC
GAAACCTTAGAAATCTACGCGCCCATCGCTAACCGTCTCGGTATCCATAACATCAAGACAGAGTTAGAAGACTTAGGTTT
CCAAGCGTATTACCCCATGCGATATCGGGTGCTTAAAGAAGTCGTTAAAGCCGCCCGTGGCAATCGCAAAGAATTGATCC
AAGGCATTGAAGCCGCTGTTTATACGCGCTTAAACGATGCGGGTATTCCAGGGAAAGTTAAAGGCCGCGAGAAAAATCTC
TACTCCATCTATAACAAGATGCAGAGTAAAGAGCTGCAATTCCAAGAAGTGATGGATATCTACGCCTTTAGGGTGATAGT
CGATTCCATCGACACTTGTTATCGCGTACTCGGCGCCATGCACGGACTGTATAAACCTCGCCCCGGTCGTTTCAAAGATT
ATATCGCTATCCCTAAAGCCAACGGCTATCAGTCACTCCATACCTCTTTGTTTGGTCCACACGGCGTGCCCGTTGAGATC
CAAATTCGTACCGAAGATATGGACCAGATGGCCGACAAAGGGGTTGCCGCACACTGGGCCTACAAAGGCGGTGCGCAAAC
AGGCCAAGGCACGACGACTCAAGTGCGTGCCCGTAAGTGGATGCAAAGCTTGTTGGAATTACAACAAAGCGCCAGCACCT
CATTCGAATTCGTTGAAAACGTGAAGACTGAACTCTTCCCAGAAGAGATTTATGTGTTCACCCCTGAAGGTCGCATTCTG
GAATTACCCGTGAATGCCACCGCGGTAGACTTTGCCTACGAAGTGCATACAGATGTGGGTAATACCTGCGTCGGTGCCCG
CGTTAACCGCCAAGCGTATCCGCTCAGCCAGCCGTTAATTTCAGGCCAAACCGTTGAAATCATTACCGCTAAAGGCGCGC
GTCCGAATGCCGCTTGGCTGAACTTTGTGGTAACAGGTAAGGCCCGCGCCAAAATCCGCCAAGTGCTGAAAAGCCTTAAG
GGTGATGACGCAATTGCGCTTGGTCGCCGCCTGCTCAACCATGCGCTCGGCAAGACTAAGCTCGACAGTATTCCGCCTGA
GCAGATTGAAAAAGTCATTCGCGATACTAAACATACCACGCTCGATTCACTGCTCGCCGACATCGGCCTAGGCAATGCCA
TGAGCATTGTTATCGCACAGCGTTTGATTGGCGATAATCTTGAGAATCAAGAAAGCCGCGACGGCCACATGATGCCAATT
CGTGGCGCCGAAGGTATGCTCGTTACCTTCGCCAACTGTTGCCGCCCAATCCCTGGTGATGCCGTAATTGCCCACGTCAG
CCAAGGTAAGGGCTTAGTGGTGCACATGGAAAACTGCGCCAACATTCGTGGTTATCAAGGCGAGCCTGACAAATATATTC
CTGTGCAATGGGATAATGTCGAAGGCGTTGATTACCAAGCCAACCTGCGGGTGGAAATCGTTAACCACCAAGGCGCACTG
GCGAAGATCACCTCGATCATTGCCGCCGAAGGCTCGAACATTCACAACCTCAGTACAGAGGAACGAGATGGCCGCGTGTA
TTTGATCAACCTGCGGATTTCGGTGAAAGACAGGATCCATTTAGCCAACGTGATGCGCCGAATTAGGGTACTGCCCGAAG
TGCTGCGTACGTCCCGTAATCGTTAA

Upstream 100 bases:

>100_bases
TGAAGCCGCTGAAATTGCAGCGGTTTCAGCTATTGCTGAAGGCCGTTCATTATAAGAGTTGTTGAGTAAACACCTGTTTA
GGTAAAAGGAGAGCTGCCAC

Downstream 100 bases:

>100_bases
TTAACTCAACATTAGAGAGAGCGTCATGGCAGAAAAAATCATCATAGCGACCGAAAACGCCCCTGCGGCCATCGGCACTT
ATTCACAGGCCGTCAAAGTC

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQIARDAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTHV
TKDDLAELFGVAVAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKTELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQGIEAAVYTRLNDAGIPGKVKGREKNL
YSIYNKMQSKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWAYKGGAQTGQGTTTQVRARKWMQSLLELQQSASTSFEFVENVKTELFPEEIYVFTPEGRIL
ELPVNATAVDFAYEVHTDVGNTCVGARVNRQAYPLSQPLISGQTVEIITAKGARPNAAWLNFVVTGKARAKIRQVLKSLK
GDDAIALGRRLLNHALGKTKLDSIPPEQIEKVIRDTKHTTLDSLLADIGLGNAMSIVIAQRLIGDNLENQESRDGHMMPI
RGAEGMLVTFANCCRPIPGDAVIAHVSQGKGLVVHMENCANIRGYQGEPDKYIPVQWDNVEGVDYQANLRVEIVNHQGAL
AKITSIIAAEGSNIHNLSTEERDGRVYLINLRISVKDRIHLANVMRRIRVLPEVLRTSRNR

Sequences:

>Translated_701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQIARDAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTHV
TKDDLAELFGVAVAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKTELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQGIEAAVYTRLNDAGIPGKVKGREKNL
YSIYNKMQSKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWAYKGGAQTGQGTTTQVRARKWMQSLLELQQSASTSFEFVENVKTELFPEEIYVFTPEGRIL
ELPVNATAVDFAYEVHTDVGNTCVGARVNRQAYPLSQPLISGQTVEIITAKGARPNAAWLNFVVTGKARAKIRQVLKSLK
GDDAIALGRRLLNHALGKTKLDSIPPEQIEKVIRDTKHTTLDSLLADIGLGNAMSIVIAQRLIGDNLENQESRDGHMMPI
RGAEGMLVTFANCCRPIPGDAVIAHVSQGKGLVVHMENCANIRGYQGEPDKYIPVQWDNVEGVDYQANLRVEIVNHQGAL
AKITSIIAAEGSNIHNLSTEERDGRVYLINLRISVKDRIHLANVMRRIRVLPEVLRTSRNR
>Mature_701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQIARDAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTHV
TKDDLAELFGVAVAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKTELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQGIEAAVYTRLNDAGIPGKVKGREKNL
YSIYNKMQSKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWAYKGGAQTGQGTTTQVRARKWMQSLLELQQSASTSFEFVENVKTELFPEEIYVFTPEGRIL
ELPVNATAVDFAYEVHTDVGNTCVGARVNRQAYPLSQPLISGQTVEIITAKGARPNAAWLNFVVTGKARAKIRQVLKSLK
GDDAIALGRRLLNHALGKTKLDSIPPEQIEKVIRDTKHTTLDSLLADIGLGNAMSIVIAQRLIGDNLENQESRDGHMMPI
RGAEGMLVTFANCCRPIPGDAVIAHVSQGKGLVVHMENCANIRGYQGEPDKYIPVQWDNVEGVDYQANLRVEIVNHQGAL
AKITSIIAAEGSNIHNLSTEERDGRVYLINLRISVKDRIHLANVMRRIRVLPEVLRTSRNR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=145, Percent_Identity=38.6206896551724, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1790082, Length=704, Percent_Identity=69.8863636363636, Blast_Score=1029, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=701, Percent_Identity=32.2396576319544, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24650996, Length=132, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 78706; Mature: 78706

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFEGLKESASGYLEPEQVELLKQAYQIARDAHEGQMRTSGEPYITHPVAVARILADMR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTHVTKDDLAELFGVAVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQGIEAAVYTRLNDAGIPGKVKGREKNL
HHHHCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
YSIYNKMQSKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTEDMDQMADKGVAAHWAYKGGAQTGQGTTTQVRARKW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
MQSLLELQQSASTSFEFVENVKTELFPEEIYVFTPEGRILELPVNATAVDFAYEVHTDVG
HHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEEEEECCCCC
NTCVGARVNRQAYPLSQPLISGQTVEIITAKGARPNAAWLNFVVTGKARAKIRQVLKSLK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHCC
GDDAIALGRRLLNHALGKTKLDSIPPEQIEKVIRDTKHTTLDSLLADIGLGNAMSIVIAQ
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
RLIGDNLENQESRDGHMMPIRGAEGMLVTFANCCRPIPGDAVIAHVSQGKGLVVHMENCA
HHHCCCCCCCCCCCCCEEEECCCCCCEEEHHHHCCCCCCCCEEEEECCCCEEEEEECCCC
NIRGYQGEPDKYIPVQWDNVEGVDYQANLRVEIVNHQGALAKITSIIAAEGSNIHNLSTE
CCCCCCCCCCCCCCEEECCCCCCCEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ERDGRVYLINLRISVKDRIHLANVMRRIRVLPEVLRTSRNR
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLFEGLKESASGYLEPEQVELLKQAYQIARDAHEGQMRTSGEPYITHPVAVARILADMR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTHVTKDDLAELFGVAVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQGIEAAVYTRLNDAGIPGKVKGREKNL
HHHHCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
YSIYNKMQSKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTEDMDQMADKGVAAHWAYKGGAQTGQGTTTQVRARKW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
MQSLLELQQSASTSFEFVENVKTELFPEEIYVFTPEGRILELPVNATAVDFAYEVHTDVG
HHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEEEEECCCCC
NTCVGARVNRQAYPLSQPLISGQTVEIITAKGARPNAAWLNFVVTGKARAKIRQVLKSLK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHCC
GDDAIALGRRLLNHALGKTKLDSIPPEQIEKVIRDTKHTTLDSLLADIGLGNAMSIVIAQ
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
RLIGDNLENQESRDGHMMPIRGAEGMLVTFANCCRPIPGDAVIAHVSQGKGLVVHMENCA
HHHCCCCCCCCCCCCCEEEECCCCCCEEEHHHHCCCCCCCCEEEEECCCCEEEEEECCCC
NIRGYQGEPDKYIPVQWDNVEGVDYQANLRVEIVNHQGALAKITSIIAAEGSNIHNLSTE
CCCCCCCCCCCCCCEEECCCCCCCEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ERDGRVYLINLRISVKDRIHLANVMRRIRVLPEVLRTSRNR
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]