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The map label for this gene is recF [H]

Identifier: 160873129

GI number: 160873129

Start: 3092

End: 4174

Strand: Direct

Name: recF [H]

Synonym: Sbal195_0003

Alternate gene names: 160873129

Gene position: 3092-4174 (Clockwise)

Preceding gene: 160873128

Following gene: 160873130

Centisome position: 0.06

GC content: 45.24

Gene sequence:

>1083_bases
ATGAGCCTGACCCGTCTCAATATCGAAGCGTTTCGCAACATTCAGTCCGCACAGTTAATCCCTGCACCTGGGATTAACTT
GATTTATGGCCAAAATGGCAGCGGCAAAACCAGTATTCTTGAGGCGATTTATTTCCTCGGTATGGGACGTTCGTTTCGCA
GCCATTTATCCCAACGAGTGATTAATAACGACGACGATAAGTTAACGCTCTTTGCGACCTTAAATTTAGCACGCGGTGAC
AGCAAAATCGGTTTGCGCCGATTTCGTAGTGGTGAGACCGAAGTGAGAATCGACGGCGAAAAAGTCAAACGTCTGTCGAC
ATTAGCCGAAACCTTACCTATTCAAGTGATCACGCCTGAAAGCTTTTCTTTACTGTTTGAAGGCCCAAAATCCCGTCGAC
AATTTATCGATTGGGGCGCATTTCATGCTGACCCACAGTTTTATGGCGCTTGGACGAATGTTCGTCGAGTTCTTAAGCAA
AGAAATCAATTGCTTAGAAATGGCTCGGCCTACAGTAATATTCAATTTTGGGACCAAGAGTTTGTGCGTTATGCCGAGCA
GGTTACTGAAATACGAAACCATTATGTAGACTCGTTAAATGAGCTACTTAAGGGTATAATCGGGGAGTTTTTACCGAGTG
TAGATGTGAAGGTTTCATTTACGCGCGGCTGGGATAGTAAAACGGATTTTGCAGAACTACTCGAAAACCAATATTCCAGA
GATTTGGCCACAGGTCATACCGTCAGCGGTCCCCATAAAGCTGACCTTAGACTTCGTGTAGGCACTTTGCCGGCGCAGGA
TGCCCTGTCCCGTGGTCAATTAAAATTATTAGTCTGTGCACTGCGTATTGCACAGGGAAAGTTGCTCAAACAACAAATAG
ATAAACACAGTATTTATCTTGTGGATGATCTTCCGTCCGAGTTGGATGCACAGCATAGGCAGCTATTGCTTAAGCAGTTA
ACGGATACCGGAGCACAAGTTTTTGTGACCGCCATCGATCCTGCTGCAATAGTCGATTCGTTACACACGCCTCCCAGTAG
GATGTTTCATGTGGAACAGGGACGTGTAACGGTAATTGAATAA

Upstream 100 bases:

>100_bases
ACTCATTTACCGATAGCCTTAGCCTGTGGCAGCGATTGAGTTGCCTGTTAAGTGTTATGAATCCTTGCCTTCGCCGTTTA
CGCGGCTTAATGAGTAATGC

Downstream 100 bases:

>100_bases
CCATTGAGAGAATAATATGTCAGAGAATAGTTACGATTCTTCGAGTATTAAGGTCCTTAAGGGCCTTGATGCAGTACGTA
AGAGACCTGGGATGTATATT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSLTRLNIEAFRNIQSAQLIPAPGINLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVINNDDDKLTLFATLNLARGD
SKIGLRRFRSGETEVRIDGEKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQ
RNQLLRNGSAYSNIQFWDQEFVRYAEQVTEIRNHYVDSLNELLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
DLATGHTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAQHRQLLLKQL
TDTGAQVFVTAIDPAAIVDSLHTPPSRMFHVEQGRVTVIE

Sequences:

>Translated_360_residues
MSLTRLNIEAFRNIQSAQLIPAPGINLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVINNDDDKLTLFATLNLARGD
SKIGLRRFRSGETEVRIDGEKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQ
RNQLLRNGSAYSNIQFWDQEFVRYAEQVTEIRNHYVDSLNELLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
DLATGHTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAQHRQLLLKQL
TDTGAQVFVTAIDPAAIVDSLHTPPSRMFHVEQGRVTVIE
>Mature_359_residues
SLTRLNIEAFRNIQSAQLIPAPGINLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRVINNDDDKLTLFATLNLARGDS
KIGLRRFRSGETEVRIDGEKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQR
NQLLRNGSAYSNIQFWDQEFVRYAEQVTEIRNHYVDSLNELLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSRD
LATGHTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYLVDDLPSELDAQHRQLLLKQLT
DTGAQVFVTAIDPAAIVDSLHTPPSRMFHVEQGRVTVIE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=46.218487394958, Blast_Score=338, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40626; Mature: 40495

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRLNIEAFRNIQSAQLIPAPGINLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCCEEECHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
INNDDDKLTLFATLNLARGDSKIGLRRFRSGETEVRIDGEKVKRLSTLAETLPIQVITPE
CCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQRNQLLRNGSAYSNIQFWDQE
CCEEEECCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
FVRYAEQVTEIRNHYVDSLNELLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
DLATGHTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYL
HHCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDDLPSELDAQHRQLLLKQLTDTGAQVFVTAIDPAAIVDSLHTPPSRMFHVEQGRVTVIE
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCHHHEEEECCCCEEEEC
>Mature Secondary Structure 
SLTRLNIEAFRNIQSAQLIPAPGINLIYGQNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCEEECHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
INNDDDKLTLFATLNLARGDSKIGLRRFRSGETEVRIDGEKVKRLSTLAETLPIQVITPE
CCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHADPQFYGAWTNVRRVLKQRNQLLRNGSAYSNIQFWDQE
CCEEEECCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
FVRYAEQVTEIRNHYVDSLNELLKGIIGEFLPSVDVKVSFTRGWDSKTDFAELLENQYSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
DLATGHTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKHSIYL
HHCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDDLPSELDAQHRQLLLKQLTDTGAQVFVTAIDPAAIVDSLHTPPSRMFHVEQGRVTVIE
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCHHHEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA