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The map label for this gene is dnaN [H]

Identifier: 160873128

GI number: 160873128

Start: 1790

End: 2890

Strand: Direct

Name: dnaN [H]

Synonym: Sbal195_0002

Alternate gene names: 160873128

Gene position: 1790-2890 (Clockwise)

Preceding gene: 160873127

Following gene: 160873129

Centisome position: 0.03

GC content: 44.6

Gene sequence:

>1101_bases
ATGAAATTCTCAATTGATAGGGATGCCCTATTAAAGCCGTTGCAATTGGTGTGTGGTGCGGTAGAAAGACGCCATAATTT
GCCCATTTTGTCTAACCTCCTTGTAGAAGTAAGTGGTCACTCACTTAAGCTGACCGGAACAGATTTAGAAGTCGAACTCG
TGGGCCAAGTTGTGGTTCACGGTGATATCGAAGAAGGTCGTACTACGGTTCCGGCTAAAAAGCTGCTCGATATTGTTAAG
TCTTTGCCAGAACAAAGCGAACTGAAAGTCGAACAACAAGATAATAAATGGTTGCTGCGTTCAGGCCGTAGCCGTTTTTC
CTTAGCGACGTTGCCCGCAGAAGAATATCCCAACGTTGAAGCCTTCCAAGCTGAGATTGAATTTAGTTTGAAGCAGGGCG
TGTTGAAGTCGATTATCGACTCAACGCAATTTTCTATGGCTAACCAAGATGTGCGTTATTACCTCAACGGTTTATTGTTT
GAAACTGAAGGTAATGTGTTGCGCGCGATTGCGACCGACGGTCACCGTTTAGCCTTAAGTCACCGCACGATTGAAGCACA
ATTACCTGAAAAACAAGTCATTGTTCCACGCAAAGGCGTAATGGAAATGGCACGTTTATTGGAATCGGACGATCTCGATA
TCGCGATTGCTATCGGTGATAATGCCATTCGTGCCACTACGGCTAACACTGTATTTACCAGTAAATTAGTCGATGGCCGC
TTCCCTGATTATCGCCGTGTATTGCCAAAGGGCGGTGATAAAATAGTGATTGCGAGTCGTAATCACCTGAAGCAAGCGCT
GACCCGCGCATCGATTTTATCGAACGAAAAATTCCGTGGTGTGCGTATTCAACTCGAAAATGGTCTGCTTAAGATCACCG
CTAACAATCCTGAACAGGAAGAAGCGGAAGAGATCATCGATGTGGAATACAACAACCAAGCCTTAGAAATTGGTTTTAAC
GTCAGTTATTTACTCGACGTGCTCAACAACCTTAAGTCAGATGATGTGCGTATCACGCTCATTGATGGTAACTCCAGCGC
CTTAATCGAAAACCATTTGGAAGAAGATTCCATGTATGTGGTTATGCCGATGCGCTTATAA

Upstream 100 bases:

>100_bases
CGTAAGATTGCTCAGCTACGGGAAGAGAGCCACGACATTAAAGAAGATTATGCTAATTTGATTAGAACCTTATCTTCTTA
AAACAAGGAATTTGGACACT

Downstream 100 bases:

>100_bases
ACCATCGATATTTACGTTACCCGTGTTTGTACCTCTGTCTTCGATGTCTATGCCGCTTAAAGGCGGCATAGTGTTTAATA
CGCAGCATTGGCCATTCCTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQVVVHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLF
ETEGNVLRAIATDGHRLALSHRTIEAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQEEAEEIIDVEYNNQALEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYVVMPMRL

Sequences:

>Translated_366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQVVVHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLF
ETEGNVLRAIATDGHRLALSHRTIEAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQEEAEEIIDVEYNNQALEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYVVMPMRL
>Mature_366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQVVVHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLF
ETEGNVLRAIATDGHRLALSHRTIEAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQEEAEEIIDVEYNNQALEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=57.9234972677596, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40948; Mature: 40948

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQVVVH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCEEEEECCCEEEEEEEEEEEE
GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEHHCCCCCEEEEECCHHHCCCCE
AFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLFETEGNVLRAIATDGHRLALS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCEEEEEECCCEEEEEE
HRTIEAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
ECEEEECCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCEEEHHHHCCC
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCCHH
EAEEIIDVEYNNQALEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYV
HHHHEEEEEECCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE
VMPMRL
EEECCC
>Mature Secondary Structure
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQVVVH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCEEEEECCCEEEEEEEEEEEE
GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEHHCCCCCEEEEECCHHHCCCCE
AFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLFETEGNVLRAIATDGHRLALS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCEEEEEECCCEEEEEE
HRTIEAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
ECEEEECCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCEEEHHHHCCC
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCCHH
EAEEIIDVEYNNQALEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYV
HHHHEEEEEECCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE
VMPMRL
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]