Click here to switch to the map view.

The map label for this gene is secDF

Identifier: 16081162

GI number: 16081162

Start: 2826900

End: 2829113

Strand: Reverse

Name: secDF

Synonym: BSU27650

Alternate gene names: 16081162

Gene position: 2829113-2826900 (Counterclockwise)

Preceding gene: 16079819

Following gene: 16079818

Centisome position: 67.11

GC content: 43.36

Gene sequence:

>2214_bases
ATGAAAAAAGGACGCTTGATTGCGTTTTTCCTTTTCGTTCTATTGATCGGCACGGGCTTGGGCTACTTTACGAAGCCTGC
CGCTAACAATATTACGTTAGGATTGGATTTGCAAGGCGGATTTGAGGTGCTGTATGATGTACAGCCTGTAAAAAAAGGTG
ACAAAATCACAAAAGACGTTCTGGTCAGCACAGTAGAGGCACTGAACCGCCGGGCCAATGTTCTCGGTGTCAGCGAACCG
AACATCCAAATTGAAGGGAATAACCGGATTCGCGTTCAGCTCGCTGGCGTGACAAACCAAAACAGAGCGCGTGAAATTTT
GGCGACTGAAGCGCAGCTTTCTTTCAGAGATGCAAATGATAAGGAACTGTTAAACGGTGCTGATCTAGTCGAAAACGGCG
CTAAACAAACTTATGATAGCACAACAAATGAGCCAATTGTCACGATTAAGCTGAAAGACGCTGATAAATTTGGTGAAGTG
ACCAAGAAGGTCATGAAAATGGCGCCAAACAACCAGCTTGTCATTTGGTTGGATTATGATAAAGGTGATTCCTTTAAGAA
AGAAGTTCAAAAAGAGCATCCTAAATTTGTATCCGCTCCAAATGTAAGTCAGGAACTAAATACAACTGATGTAAAAATTG
AAGGTCATTTCACAGCTCAAGAAGCGAAAGATTTAGCCAGCATTTTAAACGCAGGCGCACTTCCTGTGAAACTGACTGAA
AAGTATTCGACATCAGTAGGCGCGCAATTCGGCCAGCAGGCTCTCCATGATACGGTGTTTGCCGGTATTGTCGGTATCGC
AATTATTTTCTTATTTATGCTTTTCTATTACCGTCTGCCGGGATTAATCGCGGTGATTACGCTGTCTGTTTATATCTACA
TTACACTCCAGATCTTTGACTGGATGAATGCCGTACTCACGCTTCCGGGAATTGCCGCTCTCATTTTAGGTGTCGGGATG
GCTGTTGACGCCAACATTATTACCTATGAGCGGATTAAAGAAGAGCTCAAGCTAGGAAAGTCAGTCCGCTCTGCCTTCCG
TTCAGGAAACAGACGGTCATTTGCGACGATTTTTGACGCGAATATTACAACCATTATTGCAGCGGTTGTGCTCTTTATCT
TTGGGACAAGCTCTGTTAAAGGGTTTGCGACAATGCTGATCCTATCGATTTTGACAAGCTTTATCACTGCCGTTTTCTTA
TCGAGATTTCTCCTCGCTCTCCTTGTGGAAAGCAGATGGCTTGATCGGAAAAAAGGCTGGTTTGGTGTCAATAAGAAACA
TATCATGGATATTCAGGATACGGATGAAAATACAGAGCCGCATACGCCATTCCAAAAATGGGATTTCACGAGCAAACGCA
AATACTTCTTTATTTTCTCCAGTGCGGTCACGGTTGCCGGGATTATTATCCTGCTTGTGTTCAGGCTGAATCTTGGCATT
GACTTTGCAAGCGGTGCACGGATTGAAGTGCAAAGCGACCATAAGCTGACGACAGAGCAAGTTGAGAAGGATTTTGAATC
TCTGGGTATGGACCCTGATACTGTAGTTCTGTCAGGCGAAAAGAGCAATATCGGTGTTGCCCGTTTTGTCGGGGTGCCAG
ATAAAGAAACCATTGCAAAAGTAAAAACGTATTTTAAAGACAAATACGGATCTGATCCAAATGTCAGCACAGTTTCACCG
ACAGTCGGTAAGGAGCTGGCGAGAAATGCGCTGTACGCAGTTGCTATAGCTTCTATTGGCATCATTATTTACGTTTCAAT
CCGATTCGAATACAAAATGGCGATTGCTGCCATCGCCTCATTGCTATATGACGCATTCTTTATCGTCACGTTCTTCAGTA
TTACAAGGCTTGAGGTAGATGTTACATTCATCGCGGCCATCTTGACGATAATCGGGTATTCCATTAACGATACAATCGTT
ACATTTGACAGGGTCCGCGAGCATATGAAAAAGCGTAAGCCGAAAACCTTTGCCGATCTGAACCATATTGTAAACCTGAG
CCTGCAGCAAACCTTTACACGTTCAATTAACACTGTATTAACCGTTGTGATTGTTGTTGTGACATTGCTGATCTTTGGAG
CATCTTCTATTACTAACTTCTCAATTGCTTTATTGGTCGGGCTGTTAACAGGCGTTTATTCTTCTCTATACATTGCCGCA
CAAATTTGGCTTGCATGGAAAGGAAGAGAACTGAAAAAAGATTCGGCGCAATAA

Upstream 100 bases:

>100_bases
AAACGTCTAATTGGCTGGGCAGTGAAGAGGGCCAGGAAGCATTGGAAGAACTGCTGAGATAGCAGGTTGATTCGCACAGG
TTTAAGGGAGGATATACATA

Downstream 100 bases:

>100_bases
AAAATATCAGGCTGTCCTCTGCAGGGCAGCCTGTTTTTTGAAGTTTCATTGAACTTAAAATAGACATTTGCTATAATGAC
ATACGCTTACATTTAAACAT

Product: bifunctional preprotein translocase subunit SecD/SecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MKKGRLIAFFLFVLLIGTGLGYFTKPAANNITLGLDLQGGFEVLYDVQPVKKGDKITKDVLVSTVEALNRRANVLGVSEP
NIQIEGNNRIRVQLAGVTNQNRAREILATEAQLSFRDANDKELLNGADLVENGAKQTYDSTTNEPIVTIKLKDADKFGEV
TKKVMKMAPNNQLVIWLDYDKGDSFKKEVQKEHPKFVSAPNVSQELNTTDVKIEGHFTAQEAKDLASILNAGALPVKLTE
KYSTSVGAQFGQQALHDTVFAGIVGIAIIFLFMLFYYRLPGLIAVITLSVYIYITLQIFDWMNAVLTLPGIAALILGVGM
AVDANIITYERIKEELKLGKSVRSAFRSGNRRSFATIFDANITTIIAAVVLFIFGTSSVKGFATMLILSILTSFITAVFL
SRFLLALLVESRWLDRKKGWFGVNKKHIMDIQDTDENTEPHTPFQKWDFTSKRKYFFIFSSAVTVAGIIILLVFRLNLGI
DFASGARIEVQSDHKLTTEQVEKDFESLGMDPDTVVLSGEKSNIGVARFVGVPDKETIAKVKTYFKDKYGSDPNVSTVSP
TVGKELARNALYAVAIASIGIIIYVSIRFEYKMAIAAIASLLYDAFFIVTFFSITRLEVDVTFIAAILTIIGYSINDTIV
TFDRVREHMKKRKPKTFADLNHIVNLSLQQTFTRSINTVLTVVIVVVTLLIFGASSITNFSIALLVGLLTGVYSSLYIAA
QIWLAWKGRELKKDSAQ

Sequences:

>Translated_737_residues
MKKGRLIAFFLFVLLIGTGLGYFTKPAANNITLGLDLQGGFEVLYDVQPVKKGDKITKDVLVSTVEALNRRANVLGVSEP
NIQIEGNNRIRVQLAGVTNQNRAREILATEAQLSFRDANDKELLNGADLVENGAKQTYDSTTNEPIVTIKLKDADKFGEV
TKKVMKMAPNNQLVIWLDYDKGDSFKKEVQKEHPKFVSAPNVSQELNTTDVKIEGHFTAQEAKDLASILNAGALPVKLTE
KYSTSVGAQFGQQALHDTVFAGIVGIAIIFLFMLFYYRLPGLIAVITLSVYIYITLQIFDWMNAVLTLPGIAALILGVGM
AVDANIITYERIKEELKLGKSVRSAFRSGNRRSFATIFDANITTIIAAVVLFIFGTSSVKGFATMLILSILTSFITAVFL
SRFLLALLVESRWLDRKKGWFGVNKKHIMDIQDTDENTEPHTPFQKWDFTSKRKYFFIFSSAVTVAGIIILLVFRLNLGI
DFASGARIEVQSDHKLTTEQVEKDFESLGMDPDTVVLSGEKSNIGVARFVGVPDKETIAKVKTYFKDKYGSDPNVSTVSP
TVGKELARNALYAVAIASIGIIIYVSIRFEYKMAIAAIASLLYDAFFIVTFFSITRLEVDVTFIAAILTIIGYSINDTIV
TFDRVREHMKKRKPKTFADLNHIVNLSLQQTFTRSINTVLTVVIVVVTLLIFGASSITNFSIALLVGLLTGVYSSLYIAA
QIWLAWKGRELKKDSAQ
>Mature_737_residues
MKKGRLIAFFLFVLLIGTGLGYFTKPAANNITLGLDLQGGFEVLYDVQPVKKGDKITKDVLVSTVEALNRRANVLGVSEP
NIQIEGNNRIRVQLAGVTNQNRAREILATEAQLSFRDANDKELLNGADLVENGAKQTYDSTTNEPIVTIKLKDADKFGEV
TKKVMKMAPNNQLVIWLDYDKGDSFKKEVQKEHPKFVSAPNVSQELNTTDVKIEGHFTAQEAKDLASILNAGALPVKLTE
KYSTSVGAQFGQQALHDTVFAGIVGIAIIFLFMLFYYRLPGLIAVITLSVYIYITLQIFDWMNAVLTLPGIAALILGVGM
AVDANIITYERIKEELKLGKSVRSAFRSGNRRSFATIFDANITTIIAAVVLFIFGTSSVKGFATMLILSILTSFITAVFL
SRFLLALLVESRWLDRKKGWFGVNKKHIMDIQDTDENTEPHTPFQKWDFTSKRKYFFIFSSAVTVAGIIILLVFRLNLGI
DFASGARIEVQSDHKLTTEQVEKDFESLGMDPDTVVLSGEKSNIGVARFVGVPDKETIAKVKTYFKDKYGSDPNVSTVSP
TVGKELARNALYAVAIASIGIIIYVSIRFEYKMAIAAIASLLYDAFFIVTFFSITRLEVDVTFIAAILTIIGYSINDTIV
TFDRVREHMKKRKPKTFADLNHIVNLSLQQTFTRSINTVLTVVIVVVTLLIFGASSITNFSIALLVGLLTGVYSSLYIAA
QIWLAWKGRELKKDSAQ

Specific function: Required for efficient translocation of secretory pre- proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane

COG id: COG0342

COG function: function code U; Preprotein translocase subunit SecD

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the secD/secF family. SecF subfamily

Homologues:

Organism=Escherichia coli, GI1786609, Length=374, Percent_Identity=32.3529411764706, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786610, Length=291, Percent_Identity=28.5223367697594, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SECDF_BACSU (O32047)

Other databases:

- EMBL:   AF024506
- EMBL:   AL009126
- PIR:   H69704
- RefSeq:   NP_390643.1
- ProteinModelPortal:   O32047
- EnsemblBacteria:   EBBACT00000000835
- GeneID:   937938
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27650
- NMPDR:   fig|224308.1.peg.2768
- GenoList:   BSU27650
- GeneTree:   EBGT00050000000354
- HOGENOM:   HBG302175
- OMA:   TSSVKGF
- ProtClustDB:   PRK13024
- BioCyc:   BSUB:BSU27650-MONOMER
- InterPro:   IPR005791
- InterPro:   IPR022813
- InterPro:   IPR022646
- InterPro:   IPR022645
- InterPro:   IPR005665
- PRINTS:   PR01755
- TIGRFAMs:   TIGR00916
- TIGRFAMs:   TIGR00966
- TIGRFAMs:   TIGR01129

Pfam domain/function: PF07549 Sec_GG; PF02355 SecD_SecF

EC number: NA

Molecular weight: Translated: 81655; Mature: 81655

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10f9b3f8)-; HASH(0x113e582c)-; HASH(0xf0cd034)-; HASH(0x1018b674)-; HASH(0x1162a2d4)-; HASH(0x1079fb0c)-; HASH(0xd3b5424)-; HASH(0x11241f48)-; HASH(0x1161d4f8)-; HASH(0x10e75388)-; HASH(0x115fd22c)-; HASH(0x10069040)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGRLIAFFLFVLLIGTGLGYFTKPAANNITLGLDLQGGFEVLYDVQPVKKGDKITKDV
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHH
LVSTVEALNRRANVLGVSEPNIQIEGNNRIRVQLAGVTNQNRAREILATEAQLSFRDAND
HHHHHHHHHHCCCEEECCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCH
KELLNGADLVENGAKQTYDSTTNEPIVTIKLKDADKFGEVTKKVMKMAPNNQLVIWLDYD
HHHHCCHHHHHCCCHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECC
KGDSFKKEVQKEHPKFVSAPNVSQELNTTDVKIEGHFTAQEAKDLASILNAGALPVKLTE
CCCHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEHH
KYSTSVGAQFGQQALHDTVFAGIVGIAIIFLFMLFYYRLPGLIAVITLSVYIYITLQIFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMNAVLTLPGIAALILGVGMAVDANIITYERIKEELKLGKSVRSAFRSGNRRSFATIFDA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
NITTIIAAVVLFIFGTSSVKGFATMLILSILTSFITAVFLSRFLLALLVESRWLDRKKGW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FGVNKKHIMDIQDTDENTEPHTPFQKWDFTSKRKYFFIFSSAVTVAGIIILLVFRLNLGI
CCCCHHHEEECCCCCCCCCCCCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCC
DFASGARIEVQSDHKLTTEQVEKDFESLGMDPDTVVLSGEKSNIGVARFVGVPDKETIAK
EECCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHH
VKTYFKDKYGSDPNVSTVSPTVGKELARNALYAVAIASIGIIIYVSIRFEYKMAIAAIAS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEHHHHHHHHHHH
LLYDAFFIVTFFSITRLEVDVTFIAAILTIIGYSINDTIVTFDRVREHMKKRKPKTFADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHH
NHIVNLSLQQTFTRSINTVLTVVIVVVTLLIFGASSITNFSIALLVGLLTGVYSSLYIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QIWLAWKGRELKKDSAQ
HHHHEECCCCCCCCCCH
>Mature Secondary Structure
MKKGRLIAFFLFVLLIGTGLGYFTKPAANNITLGLDLQGGFEVLYDVQPVKKGDKITKDV
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHH
LVSTVEALNRRANVLGVSEPNIQIEGNNRIRVQLAGVTNQNRAREILATEAQLSFRDAND
HHHHHHHHHHCCCEEECCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCH
KELLNGADLVENGAKQTYDSTTNEPIVTIKLKDADKFGEVTKKVMKMAPNNQLVIWLDYD
HHHHCCHHHHHCCCHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECC
KGDSFKKEVQKEHPKFVSAPNVSQELNTTDVKIEGHFTAQEAKDLASILNAGALPVKLTE
CCCHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEHH
KYSTSVGAQFGQQALHDTVFAGIVGIAIIFLFMLFYYRLPGLIAVITLSVYIYITLQIFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMNAVLTLPGIAALILGVGMAVDANIITYERIKEELKLGKSVRSAFRSGNRRSFATIFDA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
NITTIIAAVVLFIFGTSSVKGFATMLILSILTSFITAVFLSRFLLALLVESRWLDRKKGW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FGVNKKHIMDIQDTDENTEPHTPFQKWDFTSKRKYFFIFSSAVTVAGIIILLVFRLNLGI
CCCCHHHEEECCCCCCCCCCCCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCC
DFASGARIEVQSDHKLTTEQVEKDFESLGMDPDTVVLSGEKSNIGVARFVGVPDKETIAK
EECCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHH
VKTYFKDKYGSDPNVSTVSPTVGKELARNALYAVAIASIGIIIYVSIRFEYKMAIAAIAS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEHHHHHHHHHHH
LLYDAFFIVTFFSITRLEVDVTFIAAILTIIGYSINDTIVTFDRVREHMKKRKPKTFADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHH
NHIVNLSLQQTFTRSINTVLTVVIVVVTLLIFGASSITNFSIALLVGLLTGVYSSLYIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QIWLAWKGRELKKDSAQ
HHHHEECCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9694879; 9384377