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The map label for this gene is exoAA

Identifier: 16081140

GI number: 16081140

Start: 4197780

End: 4198538

Strand: Reverse

Name: exoAA

Synonym: BSU40880

Alternate gene names: 16081140

Gene position: 4198538-4197780 (Counterclockwise)

Preceding gene: 50812313

Following gene: 16081139

Centisome position: 99.6

GC content: 42.82

Gene sequence:

>759_bases
ATGAAGTTGATTTCATGGAATGTAAACGGCCTGCGGGCTGTGATGAGAAAAATGGATTTCCTCTCTTATTTGAAAGAAGA
GGATGCAGATATTATATGTCTTCAGGAAACGAAGATACAGGACGGCCAGGTTGATCTCCAGCCTGAAGACTATCATGTGT
ACTGGAATTATGCAGTGAAAAAAGGATATTCCGGCACAGCTGTTTTTTCTAAGCAAGAACCGCTGCAGGTCATCTACGGT
ATCGGAGTAGAAGAGCATGACCAAGAAGGCAGAGTGATTACGCTGGAATTTGAAAACGTGTTTGTGATGACTGTTTACAC
GCCCAATTCCAGAAGGGGATTAGAGCGGATTGATTACCGCATGCAATGGGAAGAGGCTTTACTGTCTTATATTCTTGAAT
TGGATCAGAAAAAACCAGTGATTTTATGCGGTGATTTGAATGTAGCCCATCAGGAGATTGACTTGAAAAATCCAAAGGCG
AACCGGAATAACGCTGGATTTTCAGATCAAGAAAGAGAGGCATTCACTCGTTTCTTAGAAGCTGGCTTTGTTGATTCTTT
TCGTCATGTTTATCCTGATCTTGAAGGCGCTTATTCCTGGTGGTCGTATCGTGCCGGCGCCCGGGACAGAAATATTGGAT
GGCGGATTGATTATTTTGTTGTTTCTGAAAGCCTTAAGGAACAGATTGAAGATGCCAGCATATCAGCGGACGTCATGGGT
TCCGACCATTGTCCTGTTGAACTTATCATCAATATATGA

Upstream 100 bases:

>100_bases
CAAATGGCTTTACTTCCATACGTAAGCGGTGAGTAAGCTGATATGTAAAGAGCAAGGACCTTCGGGTTCTTGCTCTTTTT
TATAGGGGGGAGGATGAAAC

Downstream 100 bases:

>100_bases
TGAGATAGCAGTAAGGATTTTACACTTACTGCTTTTTTTATATGAAAAGGAGAAAGAGATGGCAAATATAAAAGAGATCG
CAAGACTGGCAAATGTATCT

Product: apurinic/apyrimidinic endonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQVDLQPEDYHVYWNYAVKKGYSGTAVFSKQEPLQVIYG
IGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKA
NRNNAGFSDQEREAFTRFLEAGFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMG
SDHCPVELIINI

Sequences:

>Translated_252_residues
MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQVDLQPEDYHVYWNYAVKKGYSGTAVFSKQEPLQVIYG
IGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKA
NRNNAGFSDQEREAFTRFLEAGFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMG
SDHCPVELIINI
>Mature_252_residues
MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQVDLQPEDYHVYWNYAVKKGYSGTAVFSKQEPLQVIYG
IGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKA
NRNNAGFSDQEREAFTRFLEAGFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMG
SDHCPVELIINI

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=49.2248062015504, Blast_Score=274, Evalue=7e-74,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=49.2248062015504, Blast_Score=274, Evalue=7e-74,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=49.2248062015504, Blast_Score=274, Evalue=7e-74,
Organism=Homo sapiens, GI18375507, Length=321, Percent_Identity=27.4143302180685, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=31.5789473684211, Blast_Score=124, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71989536, Length=260, Percent_Identity=44.6153846153846, Blast_Score=219, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=37.9310344827586, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=48.062015503876, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=48.062015503876, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): EXOA_BACSU (P37454)

Other databases:

- EMBL:   D26185
- EMBL:   AL009126
- PIR:   S66012
- RefSeq:   NP_391968.1
- ProteinModelPortal:   P37454
- SMR:   P37454
- EnsemblBacteria:   EBBACT00000001705
- GeneID:   937918
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU40880
- NMPDR:   fig|224308.1.peg.4094
- GenoList:   BSU40880
- GeneTree:   EBGT00050000001124
- HOGENOM:   HBG704164
- OMA:   DYRMKWE
- PhylomeDB:   P37454
- ProtClustDB:   CLSK888213
- BioCyc:   BSUB:BSU40880-MONOMER
- BRENDA:   3.1.11.2
- GO:   GO:0005737
- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808
- PANTHER:   PTHR22748
- TIGRFAMs:   TIGR00195
- TIGRFAMs:   TIGR00633

Pfam domain/function: PF03372 Exo_endo_phos; SSF56219 Exo_endo_phos

EC number: =3.1.11.2

Molecular weight: Translated: 29252; Mature: 29252

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00726 AP_NUCLEASE_F1_1; PS00727 AP_NUCLEASE_F1_2; PS00728 AP_NUCLEASE_F1_3; PS51435 AP_NUCLEASE_F1_4

Important sites: ACT_SITE 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQVDLQPEDYHVYWNYAVK
CEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCEEEEEEEEEE
KGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYR
CCCCCCEEECCCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHH
MQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRFLE
HHHHHHHHHHHHHHCCCCCEEEECCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
AGFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMG
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECHHHHHHHHHCCCCEEECC
SDHCPVELIINI
CCCCCEEEEEEC
>Mature Secondary Structure
MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQVDLQPEDYHVYWNYAVK
CEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCEEEEEEEEEE
KGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYR
CCCCCCEEECCCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHH
MQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRFLE
HHHHHHHHHHHHHHCCCCCEEEECCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHH
AGFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMG
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECHHHHHHHHHCCCCEEECC
SDHCPVELIINI
CCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377