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The map label for this gene is purA

Identifier: 16081094

GI number: 16081094

Start: 4155433

End: 4156725

Strand: Reverse

Name: purA

Synonym: BSU40420

Alternate gene names: 16081094

Gene position: 4156725-4155433 (Counterclockwise)

Preceding gene: 16081095

Following gene: 16081093

Centisome position: 98.6

GC content: 47.18

Gene sequence:

>1293_bases
ATGTCTTCAGTAGTTGTAGTAGGTACGCAATGGGGCGATGAAGGAAAAGGTAAAATTACAGATTTCCTATCAGAAAATGC
AGAAGTGATCGCCCGTTATCAAGGCGGAAATAACGCAGGGCATACAATCAAGTTTGACGGAATCACATATAAGCTTCACT
TAATCCCGTCTGGAATTTTCTATAAGGATAAAACGTGTGTAATCGGAAACGGAATGGTTGTAGATCCGAAAGCATTAGTC
ACAGAGCTTGCGTATCTTCATGAGCGCAACGTGAGTACAGATAACCTGAGAATCAGCAACAGAGCTCACGTCATTCTGCC
GTATCATTTGAAATTGGATGAAGTGGAAGAAGAGCGTAAAGGGGCTAACAAGATCGGCACAACGAAAAAAGGAATCGGCC
CTGCTTACATGGATAAAGCAGCCCGCATCGGAATTCGCATCGCGGATCTGTTAGACCGTGACGCGTTTGCGGAAAAGCTT
GAGCGCAATCTTGAAGAAAAAAACCGTCTTCTCGAGAAAATGTACGAGACAGAAGGGTTTAAACTTGAGGATATCTTAGA
CGAATATTATGAGTACGGACAGCAGATTAAAAAGTATGTTTGCGATACATCTGTTGTCTTAAACGATGCTCTTGATGAAG
GGCGCCGTGTATTATTTGAAGGCGCACAAGGGGTTATGCTCGATATCGACCAAGGAACATACCCGTTTGTTACGTCATCT
AACCCGGTTGCCGGCGGTGTCACGATCGGTTCTGGTGTCGGCCCGACCAAAATCAAGCACGTTGTCGGTGTATCAAAAGC
ATATACGACTCGTGTCGGCGACGGTCCTTTTCCGACTGAGCTGAAAGATGAAATCGGCGATCAAATCCGTGAAGTCGGAC
GCGAATATGGAACAACAACAGGCCGCCCGCGCCGTGTCGGCTGGTTTGACAGCGTTGTTGTCCGCCACGCCCGCCGTGTG
AGCGGAATTACAGATCTTTCTCTGAACTCAATTGACGTCCTAGCAGGAATTGAAACGTTGAAAATCTGTGTGGCGTACCG
CTACAAAGGCGAAATCATTGAAGAATTCCCAGCAAGTCTTAAGGCACTTGCTGAATGTGAGCCGGTATATGAAGAAATGC
CGGGCTGGACTGAGGATATTACAGGTGCGAAGAGCTTGAGCGAGCTTCCGGAAAATGCGCGCCATTATCTTGAGCGTGTG
TCTCAGCTGACAGGCATTCCGCTTTCTATTTTCTCTGTCGGTCCAGACCGCTCACAAACAAATGTCCTTCGCAGTGTGTA
CCGTGCGAACTAA

Upstream 100 bases:

>100_bases
AATTAATGTTCGGATTTACAATTGACTTTCTGTTTCTTCACTGATAAACTTGATTTGTTTGAATAGAATCGTTTTGAAAG
GTTAACGGAGGTGCACGGAC

Downstream 100 bases:

>100_bases
ATAGAATATGTCTGCAAGCCCCTATTTAAGGGGCTTGTTTTTGTTTGAAAGCCGCATATAAGTTGGTCTGAGAAAAAAAT
ATGAAAAAAAACCAAAAAAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFDGITYKLHLIPSGIFYKDKTCVIGNGMVVDPKALV
TELAYLHERNVSTDNLRISNRAHVILPYHLKLDEVEEERKGANKIGTTKKGIGPAYMDKAARIGIRIADLLDRDAFAEKL
ERNLEEKNRLLEKMYETEGFKLEDILDEYYEYGQQIKKYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPTKIKHVVGVSKAYTTRVGDGPFPTELKDEIGDQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGITDLSLNSIDVLAGIETLKICVAYRYKGEIIEEFPASLKALAECEPVYEEMPGWTEDITGAKSLSELPENARHYLERV
SQLTGIPLSIFSVGPDRSQTNVLRSVYRAN

Sequences:

>Translated_430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFDGITYKLHLIPSGIFYKDKTCVIGNGMVVDPKALV
TELAYLHERNVSTDNLRISNRAHVILPYHLKLDEVEEERKGANKIGTTKKGIGPAYMDKAARIGIRIADLLDRDAFAEKL
ERNLEEKNRLLEKMYETEGFKLEDILDEYYEYGQQIKKYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPTKIKHVVGVSKAYTTRVGDGPFPTELKDEIGDQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGITDLSLNSIDVLAGIETLKICVAYRYKGEIIEEFPASLKALAECEPVYEEMPGWTEDITGAKSLSELPENARHYLERV
SQLTGIPLSIFSVGPDRSQTNVLRSVYRAN
>Mature_429_residues
SSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFDGITYKLHLIPSGIFYKDKTCVIGNGMVVDPKALVT
ELAYLHERNVSTDNLRISNRAHVILPYHLKLDEVEEERKGANKIGTTKKGIGPAYMDKAARIGIRIADLLDRDAFAEKLE
RNLEEKNRLLEKMYETEGFKLEDILDEYYEYGQQIKKYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSSN
PVAGGVTIGSGVGPTKIKHVVGVSKAYTTRVGDGPFPTELKDEIGDQIREVGREYGTTTGRPRRVGWFDSVVVRHARRVS
GITDLSLNSIDVLAGIETLKICVAYRYKGEIIEEFPASLKALAECEPVYEEMPGWTEDITGAKSLSELPENARHYLERVS
QLTGIPLSIFSVGPDRSQTNVLRSVYRAN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=42.4882629107981, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=43.4679334916865, Blast_Score=329, Evalue=2e-90,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=42.0918367346939, Blast_Score=288, Evalue=8e-78,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=47.906976744186, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146555, Length=421, Percent_Identity=41.3301662707838, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI25146553, Length=421, Percent_Identity=41.3301662707838, Blast_Score=326, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=40.1891252955083, Blast_Score=332, Evalue=9e-92,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=41.726618705036, Blast_Score=331, Evalue=6e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47673; Mature: 47541

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFDGITYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCEEEEEEEEECCCE
YKDKTCVIGNGMVVDPKALVTELAYLHERNVSTDNLRISNRAHVILPYHLKLDEVEEERK
ECCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEECCHHHHHHHHC
GANKIGTTKKGIGPAYMDKAARIGIRIADLLDRDAFAEKLERNLEEKNRLLEKMYETEGF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KLEDILDEYYEYGQQIKKYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPTKIKHVVGVSKAYTTRVGDGPFPTELKDEIGDQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGITDLSLNSIDVLAGIETLKICVAYRYKGEIIEEFPASL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KALAECEPVYEEMPGWTEDITGAKSLSELPENARHYLERVSQLTGIPLSIFSVGPDRSQT
HHHHHCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
NVLRSVYRAN
HHHHHHHHCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFDGITYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCEEEEEEEEECCCE
YKDKTCVIGNGMVVDPKALVTELAYLHERNVSTDNLRISNRAHVILPYHLKLDEVEEERK
ECCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEECCHHHHHHHHC
GANKIGTTKKGIGPAYMDKAARIGIRIADLLDRDAFAEKLERNLEEKNRLLEKMYETEGF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KLEDILDEYYEYGQQIKKYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPTKIKHVVGVSKAYTTRVGDGPFPTELKDEIGDQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGITDLSLNSIDVLAGIETLKICVAYRYKGEIIEEFPASL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KALAECEPVYEEMPGWTEDITGAKSLSELPENARHYLERVSQLTGIPLSIFSVGPDRSQT
HHHHHCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
NVLRSVYRAN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA