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The map label for this gene is walK

Identifier: 16081092

GI number: 16081092

Start: 4151853

End: 4153688

Strand: Reverse

Name: walK

Synonym: BSU40400

Alternate gene names: 16081092

Gene position: 4153688-4151853 (Counterclockwise)

Preceding gene: 16081093

Following gene: 16081091

Centisome position: 98.53

GC content: 43.57

Gene sequence:

>1836_bases
ATGAATAAGGTTGGTTTTTTTCGGTCGATCCAATTTAAGATTACCTTGATTTATGTGCTTTTGATTATCATTGCCATGCA
AATCATTGGTGTGTATTTTGTCAATCAGGTAGAGAAGTCGCTGATCAGCTCTTATGAACAATCGCTGAATCAGCGAATCG
ACAACCTTTCTTATTATATCGAACAAGAATATAAGAGCGATAACGACAGTACCGTCATAAAGGATGACGTCAGCCGTATT
TTGAATGACTTCACGAAAAGTGATGAGGTTCGGGAAATTAGCTTTGTAGATAAAAGCTATGAAGTGGTCGGTTCTTCAAA
GCCGTACGGTGAGGAAGTGGCTGGGAAACAAACGACCGATCTGATTTTCAAAAGAATCTTTTCTACCAAACAGTCGTATT
TAAGAAAATATTATGACCCGAAAAGCAAAATCAGAGTTCTTATTTCCGCGAAGCCCGTCATGACTGAAAACCAAGAGGTT
GTGGGCGCGATCTACGTTGTCGCGAGTATGGAAGATGTCTTTAATCAAATGAAGACGATCAACACGATACTGGCGTCCGG
CACAGGTTTGGCACTTGTTTTGACGGCTCTTCTCGGTATTTTTCTGGCAAGAACCATTACCCACCCGCTTTCAGATATGC
GAAAGCAGGCGATGGAGCTTGCGAAAGGGAATTTCTCGAGGAAGGTTAAAAAGTATGGACATGATGAAATTGGGCAGCTG
GCCACAACGTTTAACCACTTAACGAGAGAGCTTGAAGATGCCCAGGCGATGACTGAAGGAGAACGCAGAAAGCTAGCCTC
TGTCATTGCTTATATGACAGACGGCGTTATCGCCACAAACCGAAACGGAGCGATTATTCTCTTAAACAGCCCGGCGCTGG
AACTGTTGAACGTTTCACGTGAAACAGCCTTAGAAATGCCGATTACGAGTTTGCTGGGGCTTCAAGAGAATTATACTTTT
GAAGATTTAGTGGAACAGCAGGATTCTATGCTGCTTGAAATTGAGCGCGATGATGAATTAACGGTGCTTCGCGTGAATTT
CTCTGTGATTCAAAGGGAACACGGCAAAATTGATGGTTTAATCGCTGTCATTTACGATGTAACCGAACAAGAGAAAATGG
ATCAGGAACGCAGAGAATTCGTTGCGAATGTATCACATGAGCTGCGGACGCCGCTTACGACAATGCGCAGCTATTTAGAA
GCGTTAGCTGAAGGCGCGTGGGAAAATAAAGACATTGCTCCGCGCTTCTTAATGGTGACGCAAAATGAAACAGAGCGTAT
GATCCGGCTTGTCAATGATCTGCTTCAGCTGTCGAAATTTGACAGTAAAGATTATCAATTTAACCGGGAATGGATTCAGA
TTGTCCGGTTTATGTCACTTATTATTGACCGTTTTGAAATGACAAAAGAGCAGCATGTGGAGTTTATCCGCAATCTGCCG
GACAGAGATCTGTATGTCGAGATTGACCAGGATAAAATAACGCAGGTGCTCGATAATATTATTTCTAACGCTTTAAAATA
TTCACCGGAAGGCGGACATGTCACGTTCTCGATTGATGTCAATGAAGAGGAAGAACTTCTCTATATCAGCGTGAAGGATG
AAGGGATCGGCATTCCGAAAAAAGATGTCGAAAAAGTCTTTGACCGCTTCTACAGGGTGGATAAAGCGAGAACGAGAAAG
CTTGGCGGCACCGGTCTGGGATTGGCGATTGCCAAGGAAATGGTACAGGCGCACGGTGGAGATATTTGGGCGGACAGCAT
AGAAGGAAAAGGCACGACGATTACATTTACTCTTCCATATAAAGAGGAACAAGAGGATGATTGGGATGAAGCGTGA

Upstream 100 bases:

>100_bases
CGTGAAAAAATCGAGGACAACCCGAGCCATCCAAATTGGATCGTCACACGCCGTGGTGTAGGTTATTACTTGAGAAACCC
AGAACAGGACTAATGCCCAT

Downstream 100 bases:

>100_bases
AAATATAAAAACGATATTACTTACGGTACTCGTCGTCATCAGTCTTGTTTTTACATGGGGGATATGGACGTTCCAGCCAA
ACTTTTCTGAAGGCTCATCC

Product: two-component sensor histidine kinase YycF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MNKVGFFRSIQFKITLIYVLLIIIAMQIIGVYFVNQVEKSLISSYEQSLNQRIDNLSYYIEQEYKSDNDSTVIKDDVSRI
LNDFTKSDEVREISFVDKSYEVVGSSKPYGEEVAGKQTTDLIFKRIFSTKQSYLRKYYDPKSKIRVLISAKPVMTENQEV
VGAIYVVASMEDVFNQMKTINTILASGTGLALVLTALLGIFLARTITHPLSDMRKQAMELAKGNFSRKVKKYGHDEIGQL
ATTFNHLTRELEDAQAMTEGERRKLASVIAYMTDGVIATNRNGAIILLNSPALELLNVSRETALEMPITSLLGLQENYTF
EDLVEQQDSMLLEIERDDELTVLRVNFSVIQREHGKIDGLIAVIYDVTEQEKMDQERREFVANVSHELRTPLTTMRSYLE
ALAEGAWENKDIAPRFLMVTQNETERMIRLVNDLLQLSKFDSKDYQFNREWIQIVRFMSLIIDRFEMTKEQHVEFIRNLP
DRDLYVEIDQDKITQVLDNIISNALKYSPEGGHVTFSIDVNEEEELLYISVKDEGIGIPKKDVEKVFDRFYRVDKARTRK
LGGTGLGLAIAKEMVQAHGGDIWADSIEGKGTTITFTLPYKEEQEDDWDEA

Sequences:

>Translated_611_residues
MNKVGFFRSIQFKITLIYVLLIIIAMQIIGVYFVNQVEKSLISSYEQSLNQRIDNLSYYIEQEYKSDNDSTVIKDDVSRI
LNDFTKSDEVREISFVDKSYEVVGSSKPYGEEVAGKQTTDLIFKRIFSTKQSYLRKYYDPKSKIRVLISAKPVMTENQEV
VGAIYVVASMEDVFNQMKTINTILASGTGLALVLTALLGIFLARTITHPLSDMRKQAMELAKGNFSRKVKKYGHDEIGQL
ATTFNHLTRELEDAQAMTEGERRKLASVIAYMTDGVIATNRNGAIILLNSPALELLNVSRETALEMPITSLLGLQENYTF
EDLVEQQDSMLLEIERDDELTVLRVNFSVIQREHGKIDGLIAVIYDVTEQEKMDQERREFVANVSHELRTPLTTMRSYLE
ALAEGAWENKDIAPRFLMVTQNETERMIRLVNDLLQLSKFDSKDYQFNREWIQIVRFMSLIIDRFEMTKEQHVEFIRNLP
DRDLYVEIDQDKITQVLDNIISNALKYSPEGGHVTFSIDVNEEEELLYISVKDEGIGIPKKDVEKVFDRFYRVDKARTRK
LGGTGLGLAIAKEMVQAHGGDIWADSIEGKGTTITFTLPYKEEQEDDWDEA
>Mature_611_residues
MNKVGFFRSIQFKITLIYVLLIIIAMQIIGVYFVNQVEKSLISSYEQSLNQRIDNLSYYIEQEYKSDNDSTVIKDDVSRI
LNDFTKSDEVREISFVDKSYEVVGSSKPYGEEVAGKQTTDLIFKRIFSTKQSYLRKYYDPKSKIRVLISAKPVMTENQEV
VGAIYVVASMEDVFNQMKTINTILASGTGLALVLTALLGIFLARTITHPLSDMRKQAMELAKGNFSRKVKKYGHDEIGQL
ATTFNHLTRELEDAQAMTEGERRKLASVIAYMTDGVIATNRNGAIILLNSPALELLNVSRETALEMPITSLLGLQENYTF
EDLVEQQDSMLLEIERDDELTVLRVNFSVIQREHGKIDGLIAVIYDVTEQEKMDQERREFVANVSHELRTPLTTMRSYLE
ALAEGAWENKDIAPRFLMVTQNETERMIRLVNDLLQLSKFDSKDYQFNREWIQIVRFMSLIIDRFEMTKEQHVEFIRNLP
DRDLYVEIDQDKITQVLDNIISNALKYSPEGGHVTFSIDVNEEEELLYISVKDEGIGIPKKDVEKVFDRFYRVDKARTRK
LGGTGLGLAIAKEMVQAHGGDIWADSIEGKGTTITFTLPYKEEQEDDWDEA

Specific function: Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH and ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF

COG id: COG5002

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain

Homologues:

Organism=Escherichia coli, GI1786600, Length=362, Percent_Identity=31.4917127071823, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788393, Length=234, Percent_Identity=32.4786324786325, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788549, Length=631, Percent_Identity=22.9793977812995, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI145693157, Length=263, Percent_Identity=29.277566539924, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=28.2051282051282, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=32.4894514767932, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=31.3636363636364, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI48994928, Length=272, Percent_Identity=28.3088235294118, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786912, Length=241, Percent_Identity=29.4605809128631, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=31.3559322033898, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789149, Length=240, Percent_Identity=27.9166666666667, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790551, Length=264, Percent_Identity=28.7878787878788, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787894, Length=228, Percent_Identity=28.0701754385965, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081816, Length=240, Percent_Identity=28.3333333333333, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1790861, Length=224, Percent_Identity=27.2321428571429, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=243, Percent_Identity=23.0452674897119, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790300, Length=375, Percent_Identity=25.0666666666667, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788279, Length=240, Percent_Identity=27.5, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789403, Length=211, Percent_Identity=26.0663507109005, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787374, Length=221, Percent_Identity=27.1493212669683, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YYCG_BACSU (Q45614)

Other databases:

- EMBL:   D78193
- EMBL:   AL009126
- PIR:   F70089
- RefSeq:   NP_391920.1
- ProteinModelPortal:   Q45614
- EnsemblBacteria:   EBBACT00000002363
- GeneID:   937793
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU40400
- NMPDR:   fig|224308.1.peg.4046
- GenoList:   BSU40400
- GeneTree:   EBGT00070000031815
- HOGENOM:   HBG334875
- OMA:   AMQIIGV
- PhylomeDB:   Q45614
- ProtClustDB:   CLSK888192
- BioCyc:   BSUB:BSU40400-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00304
- SMART:   SM00387
- SMART:   SM00388
- SMART:   SM00086
- SMART:   SM00091
- TIGRFAMs:   TIGR00229

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 70035; Mature: 70035

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN; PS50113 PAC; PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x138149a8)-; HASH(0x13987320)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVGFFRSIQFKITLIYVLLIIIAMQIIGVYFVNQVEKSLISSYEQSLNQRIDNLSYYI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQEYKSDNDSTVIKDDVSRILNDFTKSDEVREISFVDKSYEVVGSSKPYGEEVAGKQTTD
HHHHCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHCCCCCCCCHHHCCCHHHH
LIFKRIFSTKQSYLRKYYDPKSKIRVLISAKPVMTENQEVVGAIYVVASMEDVFNQMKTI
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NTILASGTGLALVLTALLGIFLARTITHPLSDMRKQAMELAKGNFSRKVKKYGHDEIGQL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHH
ATTFNHLTRELEDAQAMTEGERRKLASVIAYMTDGVIATNRNGAIILLNSPALELLNVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCHHHHHHCCH
ETALEMPITSLLGLQENYTFEDLVEQQDSMLLEIERDDELTVLRVNFSVIQREHGKIDGL
HHHHHCCHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEEEHHHHHHHHCCCCCHH
IAVIYDVTEQEKMDQERREFVANVSHELRTPLTTMRSYLEALAEGAWENKDIAPRFLMVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
QNETERMIRLVNDLLQLSKFDSKDYQFNREWIQIVRFMSLIIDRFEMTKEQHVEFIRNLP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRDLYVEIDQDKITQVLDNIISNALKYSPEGGHVTFSIDVNEEEELLYISVKDEGIGIPK
CCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCH
KDVEKVFDRFYRVDKARTRKLGGTGLGLAIAKEMVQAHGGDIWADSIEGKGTTITFTLPY
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECC
KEEQEDDWDEA
CCCCCCCCCCH
>Mature Secondary Structure
MNKVGFFRSIQFKITLIYVLLIIIAMQIIGVYFVNQVEKSLISSYEQSLNQRIDNLSYYI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQEYKSDNDSTVIKDDVSRILNDFTKSDEVREISFVDKSYEVVGSSKPYGEEVAGKQTTD
HHHHCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHCCCCCCCCHHHCCCHHHH
LIFKRIFSTKQSYLRKYYDPKSKIRVLISAKPVMTENQEVVGAIYVVASMEDVFNQMKTI
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NTILASGTGLALVLTALLGIFLARTITHPLSDMRKQAMELAKGNFSRKVKKYGHDEIGQL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHH
ATTFNHLTRELEDAQAMTEGERRKLASVIAYMTDGVIATNRNGAIILLNSPALELLNVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCHHHHHHCCH
ETALEMPITSLLGLQENYTFEDLVEQQDSMLLEIERDDELTVLRVNFSVIQREHGKIDGL
HHHHHCCHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEEEHHHHHHHHCCCCCHH
IAVIYDVTEQEKMDQERREFVANVSHELRTPLTTMRSYLEALAEGAWENKDIAPRFLMVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
QNETERMIRLVNDLLQLSKFDSKDYQFNREWIQIVRFMSLIIDRFEMTKEQHVEFIRNLP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRDLYVEIDQDKITQVLDNIISNALKYSPEGGHVTFSIDVNEEEELLYISVKDEGIGIPK
CCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCH
KDVEKVFDRFYRVDKARTRKLGGTGLGLAIAKEMVQAHGGDIWADSIEGKGTTITFTLPY
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECC
KEEQEDDWDEA
CCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9829949; 10878122; 11758928