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The map label for this gene is yxjB

Identifier: 16080952

GI number: 16080952

Start: 4004288

End: 4005136

Strand: Reverse

Name: yxjB

Synonym: BSU39010

Alternate gene names: 16080952

Gene position: 4005136-4004288 (Counterclockwise)

Preceding gene: 255767824

Following gene: 255767822

Centisome position: 95.01

GC content: 47.35

Gene sequence:

>849_bases
ATGAAGCGAACAGTTGATTTCAGCATGTTTCGATGCCCGCTCTGCGATTCTTCTATGGATGCGGCATCAGGGAAAAGCTT
GATTTGTACAGAACGAGGACATACATTTGATCTATCTCGGCACGGCTACGTGAACTTTCTGACGAAGCCGGTCAAAACCA
GTTACGGTGCCGAGCTTTTTGAAGCGAGGAGCAGGCTGATTGGAGAATGTGGTTTTTTTGACCCGCTCCATGATGCGATT
GCAGAGCTCATCAGTCATCCCAAATCTGGTCATGAGGCATTCACCATCTTGGACAGCGGTTGCGGTGAGGGTTCTCATTT
GAATGCGCTTTGCGGGTTTGATTATGCAGGGAAAGCAGCGATAGGAACCGGCATAGATCTTTCGAAGGACGGGATCTTAA
AAGCTTCTAAGGCATTCAAAGATCTGATGTGGGCGGTTGCCGATGTGGCGCGTGCTCCTTTTCATGATCGCCAATTTGAT
GTTGTTCTGTCTATTTTTTCGCCGTCCAATTATGCAGAGTTTCATAGACTGCTGAAGAATGATGGCATGCTCATAAAAGT
AGTGCCGCGAAGCGACTATTTAATAGAATTAAGGCAATTTTTATATACTGATTCACCAAGGCGCACGTATTCCAATACGG
CAGCAGTTGAGCGTTTTACAGCAAATGCAGCACACAGCCGTCCTGTCAGGCTGCGCTATGTTAAAACGCTTGACCAGCAG
GCGATCCACTGGCTTCTCAAGATGACGCCGCTTGCCTGGTCGGCGCCGAAAGATCGAGTCAGCCTGCTGAAAGAGATGAA
ATCAGCCGATATTACGGTTGATGTTGATATCCTAATAGGAATGAAATGA

Upstream 100 bases:

>100_bases
ACAAACTGCCAGACCTGGCCAATTTTTGAGATAGGCCTAGGTTTATTTGTCTATGCCTTTTTCATTTATCTAAAAATGAA
TGAATGGGGTGTTTTTTATC

Downstream 100 bases:

>100_bases
ATGGTAGAGGAAAGCTCCGAAAAAAGGAGCTTTCCTTTTTTAATCGGAATAGTTGGCCCACGCTTCTCGTACATATGGAA
AGGGGACGGAAACAGCCGAT

Product: ribosomal RNA methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKRTVDFSMFRCPLCDSSMDAASGKSLICTERGHTFDLSRHGYVNFLTKPVKTSYGAELFEARSRLIGECGFFDPLHDAI
AELISHPKSGHEAFTILDSGCGEGSHLNALCGFDYAGKAAIGTGIDLSKDGILKASKAFKDLMWAVADVARAPFHDRQFD
VVLSIFSPSNYAEFHRLLKNDGMLIKVVPRSDYLIELRQFLYTDSPRRTYSNTAAVERFTANAAHSRPVRLRYVKTLDQQ
AIHWLLKMTPLAWSAPKDRVSLLKEMKSADITVDVDILIGMK

Sequences:

>Translated_282_residues
MKRTVDFSMFRCPLCDSSMDAASGKSLICTERGHTFDLSRHGYVNFLTKPVKTSYGAELFEARSRLIGECGFFDPLHDAI
AELISHPKSGHEAFTILDSGCGEGSHLNALCGFDYAGKAAIGTGIDLSKDGILKASKAFKDLMWAVADVARAPFHDRQFD
VVLSIFSPSNYAEFHRLLKNDGMLIKVVPRSDYLIELRQFLYTDSPRRTYSNTAAVERFTANAAHSRPVRLRYVKTLDQQ
AIHWLLKMTPLAWSAPKDRVSLLKEMKSADITVDVDILIGMK
>Mature_282_residues
MKRTVDFSMFRCPLCDSSMDAASGKSLICTERGHTFDLSRHGYVNFLTKPVKTSYGAELFEARSRLIGECGFFDPLHDAI
AELISHPKSGHEAFTILDSGCGEGSHLNALCGFDYAGKAAIGTGIDLSKDGILKASKAFKDLMWAVADVARAPFHDRQFD
VVLSIFSPSNYAEFHRLLKNDGMLIKVVPRSDYLIELRQFLYTDSPRRTYSNTAAVERFTANAAHSRPVRLRYVKTLDQQ
AIHWLLKMTPLAWSAPKDRVSLLKEMKSADITVDVDILIGMK

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family

Homologues:

Organism=Escherichia coli, GI1788125, Length=274, Percent_Identity=31.7518248175182, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YXJB_BACSU (P42313)

Other databases:

- EMBL:   D83026
- EMBL:   AL009126
- PIR:   H70078
- RefSeq:   NP_391780.1
- ProteinModelPortal:   P42313
- SMR:   P42313
- EnsemblBacteria:   EBBACT00000001807
- GeneID:   937456
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39010
- NMPDR:   fig|224308.1.peg.3906
- GenoList:   BSU39010
- GeneTree:   EBGT00050000001283
- HOGENOM:   HBG351136
- OMA:   GFDIAKP
- PhylomeDB:   P42313
- ProtClustDB:   CLSK888133
- BioCyc:   BSUB:BSU39010-MONOMER
- InterPro:   IPR013216
- InterPro:   IPR016718
- PIRSF:   PIRSF018249

Pfam domain/function: PF08241 Methyltransf_11

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 31473; Mature: 31473

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTVDFSMFRCPLCDSSMDAASGKSLICTERGHTFDLSRHGYVNFLTKPVKTSYGAELF
CCCCCCCHHEECCCCCCCCCCCCCCEEEEECCCCEEECCCCCCHHHHHCCHHHHHHHHHH
EARSRLIGECGFFDPLHDAIAELISHPKSGHEAFTILDSGCGEGSHLNALCGFDYAGKAA
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCEE
IGTGIDLSKDGILKASKAFKDLMWAVADVARAPFHDRQFDVVLSIFSPSNYAEFHRLLKN
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHC
DGMLIKVVPRSDYLIELRQFLYTDSPRRTYSNTAAVERFTANAAHSRPVRLRYVKTLDQQ
CCEEEEEECCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCCEEEHHHHHHHHH
AIHWLLKMTPLAWSAPKDRVSLLKEMKSADITVDVDILIGMK
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECC
>Mature Secondary Structure
MKRTVDFSMFRCPLCDSSMDAASGKSLICTERGHTFDLSRHGYVNFLTKPVKTSYGAELF
CCCCCCCHHEECCCCCCCCCCCCCCEEEEECCCCEEECCCCCCHHHHHCCHHHHHHHHHH
EARSRLIGECGFFDPLHDAIAELISHPKSGHEAFTILDSGCGEGSHLNALCGFDYAGKAA
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCEE
IGTGIDLSKDGILKASKAFKDLMWAVADVARAPFHDRQFDVVLSIFSPSNYAEFHRLLKN
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHC
DGMLIKVVPRSDYLIELRQFLYTDSPRRTYSNTAAVERFTANAAHSRPVRLRYVKTLDQQ
CCEEEEEECCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCCEEEHHHHHHHHH
AIHWLLKMTPLAWSAPKDRVSLLKEMKSADITVDVDILIGMK
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377