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The map label for this gene is ywbC

Identifier: 16080888

GI number: 16080888

Start: 3937135

End: 3937515

Strand: Direct

Name: ywbC

Synonym: BSU38370

Alternate gene names: 16080888

Gene position: 3937135-3937515 (Clockwise)

Preceding gene: 16080886

Following gene: 16080891

Centisome position: 93.39

GC content: 45.14

Gene sequence:

>381_bases
ATGGCAAAACGAATTGACCATACAGGCATCATGGTAAGAGATATTAACGCGTCCATCACATTTTATGAAGAGGTACTAGG
CATGAAATTGAAGGATCGCATCACTCACACGAACGGTGTAATCGAGCTTGCTTTTCTTGGCTTTGAAGACGGCCCGGAAA
CAGAAATTGAACTGATTCAAGGCTACAGCAGCGAGCTTCCCGCTGAAGGAAAAGTGCACCACATTGCCCTGCTGACGGAT
GACATCGCGGCTGAATATACAAAAGCGGAAAAAATGAACGCAAAATTTATCGACGAGGAGATCACAACACTTCCAAACGG
ATACCGTTATTTCTATATCGAAGGCCCTGACGGTGAGTGGATTGAGTTCTTTCAGCGGTAA

Upstream 100 bases:

>100_bases
TTGCTTTCTCTTATGGTACCACAAAACGCCAAGATGTCTGCATTGACAGAAAGGAAAGGTTTTTCTACGATAGACAAAAA
GAGTTACGAGGAGGAGCAGC

Downstream 100 bases:

>100_bases
AAATAAAGAACGTACATTTTGTGATGTACGTTCTTTTTTATCTATACCAACTGATATACGTTGACTCATCGCAAATCATA
GCTTCCCAGCCCATACGCTC

Product: lyase

Products: (R)-S-Lactoylglutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MAKRIDHTGIMVRDINASITFYEEVLGMKLKDRITHTNGVIELAFLGFEDGPETEIELIQGYSSELPAEGKVHHIALLTD
DIAAEYTKAEKMNAKFIDEEITTLPNGYRYFYIEGPDGEWIEFFQR

Sequences:

>Translated_126_residues
MAKRIDHTGIMVRDINASITFYEEVLGMKLKDRITHTNGVIELAFLGFEDGPETEIELIQGYSSELPAEGKVHHIALLTD
DIAAEYTKAEKMNAKFIDEEITTLPNGYRYFYIEGPDGEWIEFFQR
>Mature_125_residues
AKRIDHTGIMVRDINASITFYEEVLGMKLKDRITHTNGVIELAFLGFEDGPETEIELIQGYSSELPAEGKVHHIALLTDD
IAAEYTKAEKMNAKFIDEEITTLPNGYRYFYIEGPDGEWIEFFQR

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWBC_BACSU (P39586)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39673
- RefSeq:   NP_391716.1
- ProteinModelPortal:   P39586
- SMR:   P39586
- EnsemblBacteria:   EBBACT00000001501
- GeneID:   937329
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38370
- NMPDR:   fig|224308.1.peg.3842
- GenoList:   BSU38370
- GeneTree:   EBGT00050000001949
- HOGENOM:   HBG704761
- OMA:   GEWIEFF
- ProtClustDB:   CLSK888106
- BioCyc:   BSUB:BSU38370-MONOMER
- InterPro:   IPR004360
- InterPro:   IPR018146

Pfam domain/function: PF00903 Glyoxalase

EC number: 4.4.1.5 [C]

Molecular weight: Translated: 14433; Mature: 14302

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00934 GLYOXALASE_I_1; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRIDHTGIMVRDINASITFYEEVLGMKLKDRITHTNGVIELAFLGFEDGPETEIELIQ
CCCCCCCCCEEEEECCCCEEEHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCHHHEEC
GYSSELPAEGKVHHIALLTDDIAAEYTKAEKMNAKFIDEEITTLPNGYRYFYIEGPDGEW
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCHHCCHHHHCCCCCEEEEEEECCCCHH
IEFFQR
HHHHCC
>Mature Secondary Structure 
AKRIDHTGIMVRDINASITFYEEVLGMKLKDRITHTNGVIELAFLGFEDGPETEIELIQ
CCCCCCCCEEEEECCCCEEEHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCHHHEEC
GYSSELPAEGKVHHIALLTDDIAAEYTKAEKMNAKFIDEEITTLPNGYRYFYIEGPDGEW
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCHHCCHHHHCCCCCEEEEEEECCCCHH
IEFFQR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: Reduced glutathione; Methylglyoxal [C]

Specific reaction: Reduced glutathione + Methylglyoxal --> (R)-S-Lactoylglutathione [C]

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377