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The map label for this gene is ywbF

Identifier: 16080885

GI number: 16080885

Start: 3934358

End: 3935557

Strand: Direct

Name: ywbF

Synonym: BSU38340

Alternate gene names: 16080885

Gene position: 3934358-3935557 (Clockwise)

Preceding gene: 255767814

Following gene: 16080886

Centisome position: 93.33

GC content: 45.83

Gene sequence:

>1200_bases
ATGCACAACTTGCAGGTGAGGCGGCATTATGCCGCGTTAAAAGGATTTTATTTGTTTGCTTTTCTCGGTACGGGCAGCAT
AATTCCCTTACTCAGTATGTATTTAACGAAGGAACAGCATTTAAGCGGATCACAGGTCGGCTTGATCATGTCACTGGGCC
CGATTGTGATGATTTTCTTTCAGCCGTTTTGGGGCATGCTGAGCGACTATACGCAAAAAACGAAAGGCCTGCTGGCGGTT
TGCACATCAATCACAGGCATCATTGGATTAGCCTATATTGCATTTGACAGTTTCCCTCTGTTTATTTTGATTGCGGCCTG
TTTTGCAGCCTTTCAAAGCACGATTATTCCACTTTCGGACAGCATTTCACTAAGGTACACACAAGAAACAAACGGCAACT
ACGGCGGAATCCGCCTGTTTGGTTCTCTCGGCTTCGGTGTCGCGGTGTTTGCCATGGGACAGGTGACGAACCAGCTGTAC
CCCATTCACGTGATCTTTATCTTTGGCTGTGCCTTTCTGTGCATTGCTGCCATATTGGCTTCACAAGTGCCCGGCCAGCA
GAAAACAACAAAGGTCAATATCCGCAAAGGATTCCGGGAGCTTATCAGCAACAAAACTTTCCTCATCTTTATGATCATCA
CCTTTACGACATTTGCTCCAAACTTAGCGAACAATACGTATTTCAGTCTTTTTCTGGACAAAAGCGGCGCGTCACTTTCC
GCTATCGGCATTTTATTTTTCATCGGTGTCATTTCAGAAATTCCATTTATGAGATTCGCCCAAACATTTATAGATAAAAT
GGGGCTGCTCAACGTCATTATGCTGTCAGGCGGAGTCTCATTATTCAGGTGGGCGCTCTATTTCACGGCGCCTTCACTAT
GGATCATTTATGCGACGGTCTTTCTTCAGGGAGTGGCCATCGGTTTATTTATCCCGGCGGCGCTTCAATATGTGAAAAAG
ATTACCCCAAGACACGTTGAAGCGACGGCGCTGACCATGTACGCCGCCATTGGCAACGGGTTCGGCAACTGGTTCTGCAC
ATTTGCAGGCGGGTACATTTTTGACTACGTGTCGATCTTCGCAGTCTACTTGCTTTTTGGCATTCTGAGCATAGCAGGCT
TTGGCCTGACATTATATTTAATGAAGGCTGAAAAAAACAAACATACACTTCATCAGCCGGCTGTTACTTTTAAACCATAA

Upstream 100 bases:

>100_bases
ACTGTCCTCTCGAGGGCAGTTTTTTTTATGTGCTTCCCCTTATTGAAAAGCTGGCGAATTCCAATATAATGTATTTGTGG
ATACCAAAGGAGGCACCCTG

Downstream 100 bases:

>100_bases
TGACTATAATGAACAAAAGAAAGGATGTGCGTTATGAACGGGCAAGAGCGAAACAGCATTTCGAAAGGGTTGCAGGTTGA
TATTGTATTGAAAGCAGATC

Product: sugar permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MHNLQVRRHYAALKGFYLFAFLGTGSIIPLLSMYLTKEQHLSGSQVGLIMSLGPIVMIFFQPFWGMLSDYTQKTKGLLAV
CTSITGIIGLAYIAFDSFPLFILIAACFAAFQSTIIPLSDSISLRYTQETNGNYGGIRLFGSLGFGVAVFAMGQVTNQLY
PIHVIFIFGCAFLCIAAILASQVPGQQKTTKVNIRKGFRELISNKTFLIFMIITFTTFAPNLANNTYFSLFLDKSGASLS
AIGILFFIGVISEIPFMRFAQTFIDKMGLLNVIMLSGGVSLFRWALYFTAPSLWIIYATVFLQGVAIGLFIPAALQYVKK
ITPRHVEATALTMYAAIGNGFGNWFCTFAGGYIFDYVSIFAVYLLFGILSIAGFGLTLYLMKAEKNKHTLHQPAVTFKP

Sequences:

>Translated_399_residues
MHNLQVRRHYAALKGFYLFAFLGTGSIIPLLSMYLTKEQHLSGSQVGLIMSLGPIVMIFFQPFWGMLSDYTQKTKGLLAV
CTSITGIIGLAYIAFDSFPLFILIAACFAAFQSTIIPLSDSISLRYTQETNGNYGGIRLFGSLGFGVAVFAMGQVTNQLY
PIHVIFIFGCAFLCIAAILASQVPGQQKTTKVNIRKGFRELISNKTFLIFMIITFTTFAPNLANNTYFSLFLDKSGASLS
AIGILFFIGVISEIPFMRFAQTFIDKMGLLNVIMLSGGVSLFRWALYFTAPSLWIIYATVFLQGVAIGLFIPAALQYVKK
ITPRHVEATALTMYAAIGNGFGNWFCTFAGGYIFDYVSIFAVYLLFGILSIAGFGLTLYLMKAEKNKHTLHQPAVTFKP
>Mature_399_residues
MHNLQVRRHYAALKGFYLFAFLGTGSIIPLLSMYLTKEQHLSGSQVGLIMSLGPIVMIFFQPFWGMLSDYTQKTKGLLAV
CTSITGIIGLAYIAFDSFPLFILIAACFAAFQSTIIPLSDSISLRYTQETNGNYGGIRLFGSLGFGVAVFAMGQVTNQLY
PIHVIFIFGCAFLCIAAILASQVPGQQKTTKVNIRKGFRELISNKTFLIFMIITFTTFAPNLANNTYFSLFLDKSGASLS
AIGILFFIGVISEIPFMRFAQTFIDKMGLLNVIMLSGGVSLFRWALYFTAPSLWIIYATVFLQGVAIGLFIPAALQYVKK
ITPRHVEATALTMYAAIGNGFGNWFCTFAGGYIFDYVSIFAVYLLFGILSIAGFGLTLYLMKAEKNKHTLHQPAVTFKP

Specific function: Responsible For Transport Of Beta-Galactosides Into The Cell, With The Concomitant Import Of A Proton (Symport System). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWBF_BACSU (P39589)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39676
- RefSeq:   NP_391713.1
- ProteinModelPortal:   P39589
- EnsemblBacteria:   EBBACT00000003182
- GeneID:   937325
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38340
- NMPDR:   fig|224308.1.peg.3839
- GenoList:   BSU38340
- GeneTree:   EBGT00050000000132
- HOGENOM:   HBG475865
- OMA:   ITITHRM
- ProtClustDB:   CLSK626743
- BioCyc:   BSUB:BSU38340-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR016196

Pfam domain/function: SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 44010; Mature: 44010

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12b55110)-; HASH(0x133099ac)-; HASH(0x130de310)-; HASH(0xdd16c24)-; HASH(0x12727f74)-; HASH(0x12fc6f3c)-; HASH(0xd13305c)-; HASH(0x120c30fc)-; HASH(0x1338ccb8)-; HASH(0x130c5c18)-; HASH(0x1335444c)-; HASH(0x13238e64)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLQVRRHYAALKGFYLFAFLGTGSIIPLLSMYLTKEQHLSGSQVGLIMSLGPIVMIFF
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
QPFWGMLSDYTQKTKGLLAVCTSITGIIGLAYIAFDSFPLFILIAACFAAFQSTIIPLSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
SISLRYTQETNGNYGGIRLFGSLGFGVAVFAMGQVTNQLYPIHVIFIFGCAFLCIAAILA
CEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SQVPGQQKTTKVNIRKGFRELISNKTFLIFMIITFTTFAPNLANNTYFSLFLDKSGASLS
HCCCCCCCHHEEHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEECCCCCHH
AIGILFFIGVISEIPFMRFAQTFIDKMGLLNVIMLSGGVSLFRWALYFTAPSLWIIYATV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHH
FLQGVAIGLFIPAALQYVKKITPRHVEATALTMYAAIGNGFGNWFCTFAGGYIFDYVSIF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHH
AVYLLFGILSIAGFGLTLYLMKAEKNKHTLHQPAVTFKP
HHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MHNLQVRRHYAALKGFYLFAFLGTGSIIPLLSMYLTKEQHLSGSQVGLIMSLGPIVMIFF
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
QPFWGMLSDYTQKTKGLLAVCTSITGIIGLAYIAFDSFPLFILIAACFAAFQSTIIPLSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
SISLRYTQETNGNYGGIRLFGSLGFGVAVFAMGQVTNQLYPIHVIFIFGCAFLCIAAILA
CEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SQVPGQQKTTKVNIRKGFRELISNKTFLIFMIITFTTFAPNLANNTYFSLFLDKSGASLS
HCCCCCCCHHEEHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEECCCCCHH
AIGILFFIGVISEIPFMRFAQTFIDKMGLLNVIMLSGGVSLFRWALYFTAPSLWIIYATV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHH
FLQGVAIGLFIPAALQYVKKITPRHVEATALTMYAAIGNGFGNWFCTFAGGYIFDYVSIF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHH
AVYLLFGILSIAGFGLTLYLMKAEKNKHTLHQPAVTFKP
HHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377